Formula Syntax

TRANSFORMATIONS
Symbol or Function Description Example
+ Addition CTL1+0.100
- Subtraction or negation BLK-0.010
/ Division CTL1/CTL2
* Multiplication STD1*0.10
() Represents inclusion (Note: Gen5 limits (CTL1/CTL2)*100
transformations to a maximum of 9 nested pairs
of parentheses.)

DS1 Represents Data Set # 1 DS1.X

DS2 Represents Data Set # 2 DS2.X
DS3 Represents Data Set # 3 DS3.X
DS4 Represents Data Set # 4 DS4.X
X or x The value of the current well X*100
Well Coordinates The value of a specific well A1/B1
Well Identifier The mean of the specified Well Identifier BLK, SPL1
Well Identifiers use the following syntax: SPL1:1
<prefix><index>:<Dilution Index>
It is important to note that the last number is the SPL_ALL
Dilution index not the dilution value.
In addition, the well Index can be replaced by the STD
generic index “_ALL” which returns the mean of SPL_ALL:2
all indexes of the well type. The “_ALL” suffix is CTRL1:3
limited to well types sample and sample control.
For standards, the mean of all standards can be
obtained by use of the standard prefix (e.g.
STD).
MEAN(<ID>) or The mean of the specified well identifier or MEAN(POS1)
MEAN(<x>;<y>;z>;…) variables (Up to 10 variables) MEAN(A1;A2)
SD(<ID>) The standard deviation of the specified well SD(BLK)
SD(<x>;<y>;z>;…) identifier or variables (Up to 10 variables) SD(A1;B1;C1)
CV(<ID>) The coefficient of variation of the specified well CV(STD1)
CV(<x>;<y>;z>;…) identifier or variables (Up to 10 variables) CV(A1;A2;A3;A4)
ROUND(<x>, <y>) Rounds <x> to the <y> number of significant ROUND(X;4)
digits

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TRUNCATE(<x>, <y>) Truncates <x> to the <y> number of significant TRUNCATE(X;5)
digits
CONC(<ID>) or CONC(X) Returns the actual concentration value of the CONC(STD1)
current well (X) or the well identifier CONC(X)
DIL(<ID>) or DIL(X) Returns the actual dilution value of the current DIL(X)
well (X) or the well identifier DIL(SPL1:2)
LOG(<x>) Represents the LOG10 function LOG(SPL10)
POW(<x>;<y>) Raises <x> to the power of <y> POW(X;3)
SQRT(<x>) Calculates the square root of <x> SQRT(A1*X)
MIN(<x>;<y>;<z>;…) Determines the minimum of the defined variables MIN(CV(CTL1);CV(CTL2))
(Up to 10 variables)
MAX(<x>;<y>;<z>;…) Determines the maximum of the defined MAX(CV(CTL1);CV(CTL2))
variables (Up to 10 variables)

KINETIC WELL ANALYSIS

R# or r# The value of a specific kinetic read R1
+ Addition R1+0.100
- Subtraction or negation R2-0.010
/ Division R2/R1
* Multiplication R1*0.10
() Represents inclusion (R2/R1)*100
Numeric value Represents a value 0.500, 54000
MEAN(<x>;<y>;<z>;…) The mean of a series of numbers MEAN(R1;R2;R3)
SD(<x>;<y>;<z>;…) The standard deviation of a series of numbers SD(R1;R2;R3)
CV(<x>;<y>;<z>;…) The coefficient of variation of a series of numbers CV(R1;R2;R3)

ROUND(<x>; <y>) Rounds <x> to the <y> number of significant ROUND(R4;4)

digits
TRUNCATE(<x>; <y>) Truncates <x> to the <y> number of significant TRUNCATE(R4;5)
digits
LOG(<x>) Represents the LOG10 function LOG(R1)
POW(<x>;<y>) The value of <x> raised to the power of <y> POW(R2;3)
SQRT(<x>) The square root of <x> SQRT(R1*R2)
MIN(<x>;<y>;<z>;…) The minimum of the defined variables MIN(R1;R2;R3)
MAX(<x>;<y>;<z>;…) The maximum of the defined variables MAX(R1;R2;R3)

SPECTRUM WELL ANALYSIS

W# or w# The value of a specific spectrum read W1
+ Addition W1+0.100
- Subtraction or negation W2-0.010
/ Division W2/W1
* Multiplication W1*0.10
() Represents inclusion (W2/W1)*100
Numeric value Represents a value 0.500, 54000
MEAN(<x>;<y>;<z>;…) The mean of a series of numbers MEAN(W1;W2;W3)
SD(<x>;<y>;<z>;…) The standard deviation of a series of numbers SD(W1;W2;W3)
CV(<x>;<y>;<z>;…) The coefficient of variation of a series of numbers CV(W1;W2;W3)

2
ROUND(<x>; <y>) Rounds <x> to the <y> number of significant ROUND(W4;4)
digits
TRUNCATE(<x>; <y>) Truncates <x> to the <y> number of significant TRUNCATE(W4;5)
digits
LOG(<x>) Represents the LOG10 function LOG(W1)
POW(<x>;<y>) The value of <x> raised to the power of <y> POW(W2;3)
SQRT(<x>) The square root of <x> SQRT(W1*W2)
MIN(<x>;<y>;<z>;…) The minimum of the defined variables MIN(W1;W2;W3)
MAX(<x>;<y>;<z>;…) The maximum of the defined variables MAX(W1;W2;W3)

CUTOFF, VALIDATION AND INTERPOLATION FORMULAS

+ Addition CTL1+0.100
- Subtraction or negation BLK-0.010
/ Division CTL1/CTL2
* Multiplication STD1*0.10
() Represents inclusion (CTL1/CTL2)*100
Numeric value Represents a value 0.500, 54000
Well coordinates The value of a specific well A1; F4; H12
Well Identifier The mean of the specified Well Identifier BLK, SPL1
Well Identifiers use the following syntax: SPL1:1
<prefix><index>:<Dilution Index>
It is important to note that the last number is SPL#
the Dilution index not the dilution value.

In addition, the well index can be replaced by STD

the generic index “_ALL” which returns the
mean of all indexes of the well identifier. The
“_ALL” suffix is limited to well types sample and
sample control. For standards, the mean of all
standards can be obtained by use of the
standard prefix (e.g. STD).

The generic index “#” can be used which SPL_ALL:2

returns the mean value of each separate well CTRL1:3
index. The dilution index can also be replaced SPL#:#
by the generic index “#” which returns the
mean value of the well index at each
dilution/concentration index.
MEAN(<ID>) or The mean of the specified well identifier or MEAN(POS1)
Mean(<x>;<y>;z>;…) variables (Up to 10 variables) MEAN(A1;A2)
SD(<ID>) or The standard deviation of the specified well SD(BLK)
identifier or variables (Up to 10 variables)
SD(<x>;<y>;z>;…) SD(A1;A2)
CV(<ID>) or The coefficient of variation of the specified well CV(STD1)
identifier or variables (Up to 10 variables)
CV(<x>;<y>;z>;…) SD(A1;A2)
ROUND(<x>, <y>) Rounds <x> to the <y> number of significant ROUND(SPL1;4)
digits
TRUNCATE(<x>, <y>) Truncates <x> to the <y> number of significant TRUNCATE(STD1;5)
digits
CONC(<ID>) Returns the actual concentration value of the CONC(STD1)
well identifier

3
DIL(<ID>) Returns the actual dilution value of the well
identifier DIL(SPL1:2)
LOG(<x>) Represents the LOG10 function LOG(SPL10)
POW(<x>;<y>) The value of <x> raised to the power of <y> POW(STD1;3)
SQRT(<x>) The square root of <x> SQRT(A1*B1)

MIN(<x>;<y>;<z>;…) The minimum of the defined variables MIN(CV(CTL1);CV(CTL2))

MAX(<x>;<y>;<z>;…) The maximum of the defined variables MAX(A1;B1;C1)

CURVE(<x>;<y>) X must be the name of a curve that is defined in CURVE(Curve;R2)

CURVE(<x>;<y>;<z>) a Curve Fit step prior to the current Validation CURVE(StdCurve;B;95_MIN)
Step.
Returns the specified curve metric

Rev. 3/21/07