Department of Biological Sciences

Visayas State University

Visca, Baybay, Leyte

Name: Renomeron, Dale Marie P. Instructor: Prof. Marita I. Galinato
Lab. Schedule: MW (1:00 4:00) Date Submitted: Jan. 6, 2013

Exercise 4
Smear Techniques: Meiosis
























(Zoomed view)







Fig. 1
Smeared anther of a
Bangka-bangkaan flower bud
(Rhoeo discolor)

Prophase I

Total Magnification: 800x

















































(Zoomed view)





Fig. 2
Smeared anther of a
Bangka-bangkaan flower bud
(Rhoeo discolor)

Metaphase I

Total Magnification: 800x
















































(Zoomed View)






Fig. 3
Smeared anther of a
Bangka-bangkaan flower bud
(Rhoeo discolor)

Anaphase I

Total Magnification: 800x


























(Zoomed View)




Fig. 4
Smeared anther of a
Bangka-bangkaan flower bud
(Rhoeo discolor)

Prophase II, Telophase I

Total Magnification: 800x










































(Zoomed View)












Fig. 5
Smeared anther of a
Bangka-bangkaan flower bud
(Rhoeo discolor)

Metaphase II

Total Magnification: 800x





Guide Questions:

From which collections (size, stage of development and time of day) did you observed dividing
cells?
I observed dividing cells from the smallest buds which we collected during 12:00 noon.
Did you observe all the stages in a single slide?
Unfortunately, I did not observe all the stages in just a single slide. However, I had a one
slide which contains Prophase I, Metaphase I, Anaphase I, and Prophase II, Telophase I.
But it lacks Metaphase II, Anaphase II, and Telophase II. And so, I prepared another slide
which abled me to observe only the Metaphase II and still lacking of Anaphase and
Telophase II.
What difficulties have you encountered during preparation of the slide?
There were lots of difficulties that I encountered during the preparation of my slide:
1) It was very hard to squash the anthers of the flower bud with the use of the base
of the bent needle due to the size of it. When I started smashing the anthers, some
of its particles will stick to the bent needle and some will no longer be identified due to
the acetocarmine stain that was very red. However, when I already learned about the
technique in which (just like from the onion root tip in Exercise # 3) you must smash
the anthers gently with the base of the bent needle and in order to smashed it more,
just put the cover slide and tap it with the use of the pencil eraser to avoid the
breakage of the slide or cover slip as well.
2) Some of my cells are over stained with acetocarmine stain. But, I only put some of
acetic acid in order to destain my specimen.
3) Some of my cells are burned by the alcohol lamp because of the over-heating. I
have to prepare a new slide all over again.
4) It was very difficult to find some of the stages particularly the Metaphase II,
Anaphase II, and the Telophase II.
What specific modification of the standard method could you suggest for your specific
species?
Maybe, all I could suggest of a specific modification of the standard method for my specific
species is that you must know or be familiarized with your specimen. For example, just like
this activity which was the preparation of smears for meiotic studies. Of course, its
important that you knew of what was the right time of collecting the buds as well as the
right sizes of it to be collected. Through this, you can possibly observe all the stages that
you wanted to observe.