{Genetic engineering is possible, because

~We can cut DNA at specific sites by endonucleases like DNAase I

=Restriction endonucleases purified from bacteria can be used in vitro
~The phenomenon of transduction in bacteria is well understood
~We can see DNA by electron microscope
}
{
Restriction endonucleases (Restriction enzymes) are used for
Genetic Engineering
~Used to invitro DNA synthesis
=Used in genetic engineering
~Synthesized by bacteria
~Present in mammalian cells for degradation of DNA
}
{Transgenic animals are those which are
~Foreign RNA in all its cell
~Foreign DNA in some of its cells
=Foreign DNA in all its cells
~Both a and b
}
{The first transgenic plant was
=Tobacco
~Pea
~Flax
~Cotton
}
{Which of the following is related to genetic Engineering?
~Plastid
=Plasmid
~Heterosis
~Mutation
}
{First cloned animal
=Dolly sheep
~Dog
~Mule
~Cat
}
{Which of the following bases in plasmids discovered so far
~50 kilo base
=500 kilo base
~5 kilo base
~500 kilo base
}
{Plasmid has been used as vector because
plasmid
~It has antibiotic resistance gene
=It is circular DNA which have capacity to join to eukaryotic DNA
~Both ends show replication
~It can move between prokaryotic and eukaryotic cells
}
{The bacteria generally used for genetic engineering is
~Bacillus
~Pseudomonas
~Clostridium
=Agrobacterium
}
{Which of the following cut the DNA from specific places
=E.coli restriction endonuclease II
~Ligase
~Exonuclease
~Alkaline phosphatase
}
{
Cosmids can carry genomic fragement upto..
~ 30kb
~12kb
= 45kb
~100kb
}
{
The length of human genome is around..
~ 200MB
~ 1000MB
=3000MB
~500MB
}
{
What is the role of DNA ligase ?
~cleavage of the DNA fragement
~ synthesis of DNA fragment
=Joining of two ends of DNA fragement
~ All of the above
}
{
Vent is an example of .
~ RNAse
~DNAse
~ Restriction Enzyme
=Taq polymerase
}
{
Taq polymerase is isolated from which bacterial species
=Thermus aquanticus
~Bacillus substlis
~Aspergillus niger
~Penicillum noctatum
}
{
Significance of Real time PCR
=Quantification of DNA can be determined in each step
~Used for amplification of c-DNA
~ used for amplification of m-RNA
~all of the above
}
{
pUC contains which marker gene
~leuZ
=LacZ
~Ter
~All of the above
}
{
Size of pBR322 is around
~2kb
~4kb
=4.8kb
~6kb
}
{
GFP stands for
= Green fluorescent protein
~ Green florochrome protein
~Green fusion protein
~ all of the above
}
{
The DNA shows maximum absorbance at ..
~230nm
~280nm
=260nm
~300nm
}
{
Screening strategy used for pUC cloning vector is
~red white selection
~blue black selection
=blue white screening
~ none of the above
}
{
EcoR1 produces
=Sticky
~blunt
~both
~none of the above
}
{
What is nature of lamda phages
=lysogenic
~lytic
~both 1 &2
~none of the above
}
{
Single standed DNA can be cloned using which phage vector
~Lamda
= M13
~phagemid
~cosmid
}
{
Maximum size of fragement to be cloned using plasmid.
~10kb
~20kb
=5kb
~100kb
}
{
Which enzyme can convert m-RNA to c-DNA
=Reverse Transcriptase

~RNA Polymerase
~Restriction endonuclease
~ all of the above
}