EFEKANTIMIKROBAEKSTRAKBIJIJINTANHITAM(NigellasativaL)

TERHADAPSalmonellatyphi
Oleh:DWIINDAHFATMAWATI(04020015)
Medical
Dibuat:20091126,dengan3file(s).
Keywords: Ekstrakbijijintanhitam(NigellasativaL)
Salmonellatyphimerupakanagenpenyebabpenyakitdemamtifoidyangmasihseringdijumpai
secaraluasdiberbagainegaraberkembangdanmemilikibentukinfeksipalingseriusdibanding
genusyanglainnyakarenaseringmenyebabkankomplikasiberupaperdarahandanperforasi
usus.Berdasarkanpenelitianterdahulu,bijijintanhitammengandungzataktifantimikrobayang
terdiriatasvolatileoil,triterpenesaponindanflavonoid.Penelitianinimerupakantrue
experimentsdenganmenggunakanPosttestOnlyControl.Konsentrasiekstrakbijijintanhitam
yangdigunakanialah100%,50%,25%,12,5%,6,25%,3,125%,1,56%,0,78%dan2kontrol
(kontrolbahandankontrolkuman).AnalisadatamenggunakanonewayANOVA,korelasi,dan
regresi.HasilpenelitianKHM(KadarHambatMinimum)padakonsentrasi3,125%,danKBM
(KadarBunuhMinimum)padakonsentrasi6,25%.HasilujionewayANOVAmenunjukkan
adanyaperbedaanyangbermaknaantarperlakuan(p=0,000)dan(p=0,001)padazonainhibisi.
Semakintinggikonsentrasiekstrakbijijintanhitam,semakinbesarkemampuanmenghambat
danmembunuhbakteriSalmonellatyphi(r=0,874).Semakintinggikonsentrasiekstrak
semakinlebarzonainhibisiyangterbentuk(r=0,898).Pemberiankonsentrasiekstrakbijijintan
hitamberpengaruhterhadappenurunanjumlahkolonibakteriSalmonellatyphiperml(106)(R2
=76.4%).Ekstrakbijijintanhitammempunyaiefekantimikrobaterhadappertumbuhan
Salmonellatyphi.
Salmonellatyphiiscausativeagentoftyphoidfever,oftenmetindevelopingcountriesandhave
seriousinfectionformtotheothergenusbecausemakecomplicationforexamplebleedingand
perforasiofintestine.Accordingtothepreviousresearch,blackseedscontainsactive
antimicrobialwhicharevolatileoil,triterpenesaponinandflavonoid.Theresearchistrue
experimentsusingPosttestOnlyControl.Theconcentrationofblackseedsextractthatusedare
100%,50%,25%,12.5%,6.25%,3.125%,1.56%,0.78%and2controls(materialcontroland
germcontrol).AnalysisofdatausingonewayANOVA,correlation,andregression.MIC
(MinimalInhibitoryConcentration)fromtheexperimentison3.125%concentration,andMBC
(MinimalBactericidalConcentration)ison6.25%concentration.OnewayANOVAtestshows
significantdifferencebetweenblackseedsextractconcentration(p=0.000)andinhibisizone(p
=0.001).Thehigherblackseedsextractconcentration,thelesserthenumberofSalmonellatyphi
collonies(r=0.874).Thehigherblackseedsextractconcentration,thewiderabilitytomake
inhibisizone(r=0.898).BlackseedsextractinfluencesreducelargeamountofSalmonellatyphi
colonies(R2=76.4%).BlackseedsextracthaveantimicrobialeffecttogrowthofSalmonella
typhi.