1

Plant Tissue Culture Basal Salts and Media

Product
Number
Product Description Product Notes
Package
Size
A267 ANDERSON BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Anderson (1978, 1980).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
B144 BANANA AGS BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
to culture bananas.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M462 Musa (Banana) Medium: See MS-Based Plant Specifc Medium Section
C206 CAPE SUNDEW/ VENUS FLY TRAP
MULTIPLICATION BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
for the multiplication of carnivorous plants.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C216 CAPE SUNDEW/ VENUS FLY TRAP
PRETRANSPLANT BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
for the growth of carnivorous plants.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C212 CARROT CALLUS INITIATION BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
to initiate carrot callus from pith tissue.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C222 CARROT SHOOT DEVELOPMENT BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
to initiate shoots from carrot callus.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C287 CHEE & POOL C2d VITIS BASAL MEDIUM
Contains the macro- and micronutrients as described by Chee & Pool (1987).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C167 CHU N6 BASAL MEDIUM w/ VITAMINS
Contains the macro- and micronutrients, and vitamins as described by Chu (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C416 CHU N6 BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Chu (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
C149 CHU N6 VITAMIN SOLUTION (1000x)
See Vitamin Section (following the MS-based media section)
D146 DCR BASAL SALT MIXTURE
Contains macro- and micronutrients as described by Gupta & Durzan (1985).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
O
ur Plant Tissue Culture Tested basal salts and media
have been formulated according to the references
cited in their respective product listings. These basal salts
and media are then manufactured and packaged in en-
vironmentally controlled facilities. Our biological testing
ensures that each lot produces culture growth consistent
with prior lots.
On the following pages are many popular basal salt and
media formulas followed by Murashige and Skoog-based
formulations. Vitamin listings and formulations complete
this section. Please contact us with inquiries about larger
package sizes or custom formulations.
Instructions for basal salt, media, and vitamin preparation
are provided in the Technical Information Section of this
catalog and on our web site: www.phytotechlab.com.
B
asal Salt vs Medium... our defnition
A Basal Salt Mixture contains macro- and micronutri-
BB
ents, but no vitamins, carbohydrates, or growth regulators.
A Basal Medium contains the macro- and micronutrients,
but is incomplete as it typically lacks an organic compo-
nent: vitamins, carbohydrates, or growth regulators. A
Medium typically is complete and requires no additional
components (except gelling agent, if desired). A Modifed
Basal Salt, Basal Medium, or Medium indicates that one
or more of the components varies in concentration or form
from that originally published.
2
All components expressed in
mg/L
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COMPONENT A267 B144 C206 C216 C212 C222 C287 C167 C416 D146
Aluminum Chloride6H
2
O
Ammonium Nitrate 400 1650 400 825 1650 400
Ammonium Phosphate, Monobasic
Ammonium Sulfate 134 134 463 463
Boric Acid 6.2 6.2 6.2 3.1 3 3 6.2 1.6 1.6 6.2
Calcium Chloride, Anhydrous 332.2 333 332.2 166.5 113.24 113.24 125.33 125.33 64.14
Calcium Nitrate 492.3 386.31
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.0125 0.025 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.0125 0.025 0.025 0.025 0.25
Na
2
EDTA2H
2
O 74.5 74.5 37.26 37.26 37.3 37.25 37.25 37.3
Ferric Chloride
Ferric Sodium EDTA 36.7 18.35
Ferrous Sulfate7H
2
O 55.7 55.7 27.8 27.8 27.8 27.85 27.85 27.8
Magnesium Sulfate, Anhydrous 180.7 181 180.7 90.5 122.09 122.09 180.6 90.37 90.37 180.7
Manganese Chloride4H
2
O
Manganese SulfateH
2
O 16.9 16.9 16.9 8.45 10 10 0.845 3.3 3.3 22.3
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.125 0.25 0.25 0.25 0.25
Molybdenum Trioxide
Nickel Chloride6H
2
O 0.025
Nickel Sulfate6H
2
O
Potassium Chloride
Potassium Iodide 0.3 0.83 0.415 0.75 0.75 0.8 0.8 0.83
Potassium Nitrate 480 1900 480 950 2500 2500 1900 2830 2830 340
Potassium Phosphate, Dibasic
Potassium Phosphate, Monobasic 170 85 170 400 400 170
Potassium Sulfate
Sodium Nitrate
Sodium Phosphate Monobasic 330.6 295 380 150 150
Sodium Sulfate
Zinc Nitrate6H
2
O
Zinc Sulfate7H
2
O 8.6 8.6 8.6 4.3 2 2 8.6 1.5 1.5 8.6
Adenine Hemisulfate 80
Glycine (Free Base) 2
2,4-Dichlorophenoxyacetic Acid 1
6-,-Dimethylallylaminopurine (2iP) 10 1
Indole-3-acetic Acid 1
Kinetin 0.2
L(-)Malic Acid
myo-Inositol 100 100 50 100 100 10
Nicotinic Acid (Free Acid) 1 1 1 0.5
PyridoxineHCI 1 1 1 0.5
ThiamineHCI 0.4 0.4 0.2 10 10 1 1
Grams of powder to prepare 1 liter 1.89 4.71 2.12 2.20 3.21 3.21 4.49 3.99 3.98 1.64
pH0.5 at RT 3.7 5.7 3.5 4.3 3.0 4.0 4.0 4.0 4.1 4.0
NS = No Specifcation Established rev 11/2005
3
Plant Tissue Culture Basal Salts and Media (continued)
Product
Number
Product Description Product Notes
Package
Size
D190 DKW BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Driver & Kuniyuki (1984) and
McGranahan, et al (1987).
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
D191 DKW BASAL MEDIUM
Contains 10 g/L Sucrose; without Vitamins
Contains macro- and micronutrients as described by Driver & Kuniyuki (1984) and
McGranahan, et al. (1987).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
D189 DKW BASAL MEDIUM
Contains 30 g/L Sucrose; without vitamins
Contains macro- and micronutrients as described by Driver & Kuniyuki (1984) and
McGranahan, et al. (1987).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
E330 ERIKSSON VITAMIN SOLUTION (1000x)
See Vitamin Section (following the MS-based media section)
G768 GAMBORG BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Gamborg, et al. (1968).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
G398 GAMBORG B-5 BASAL MEDIUM
Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al.
(1968).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
G359 GAMBORG (PRL-4-DM) LONG BASAL MEDIUM
Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al.
(1966).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
G249 GAMBORG VITAMIN POWDER (1000x)
See Vitamin Section (following the MS-based media section)
G219 GAMBORG VITAMIN SOLUTION (1000x)
See Vitamin Section (following the MS-based media section)
G371 GRESSHOFF & DOY BASAL MEDIUM
Contains the macro- and micronutrients as described by Gresshoff & Doy (1974).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
H393 HELLER BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Heller (1953).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
H396 HELLER/ WHITE MODIFIED BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Heller (1953) and White
(1963). Without Molybdenum Trioxide
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
H353 HOAGLAND MODIFIED BASAL SALT MIXTURE
Contains Ferrous Sulfate
Contains the macro- and micronutrients as described by Hoagland and Arnon (1938).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
HOSTA Media
See MS-Based Plant-Specifc Media (following this section)
K413 KAO & MICHAYLUK BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Kao & Michayluk (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
K427 KAO & MICHAYLUK MODIFIED BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and organic acids as described by
Kao & Michayluk (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
K421 KAO & MICHAYLUK VITAMIN SOLUTION (100x)
See Vitamin Section (following the MS-based media section)
4
All components
expressed in mg/L
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COMPONENT D190 D191 D189 G768 G398 G359 G371 H393 H396 H353 K413 K427
Aluminum Chloride6H
2
O 0.0543 0.03
Ammonium Nitrate 1416 1416 1416 1000 600 600
Ammonium Phosphate,
Monobasic
115.03
Ammonium Sulfate 134 134 200
Boric Acid 4.8 4.8 4.8 3 3 3 0.3 1 1.24 2.86 3 3
Calcium Chloride, Anhydrous 112.5 112.5 112.5 113.24 113.24 113.24 56.7 453 453
Calcium Nitrate 1367 1367 1367 241.2 300 656.4
Cobalt Chloride6H
2
O 0.025 0.025 0.25 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.25 0.25 0.25 0.025 0.025 0.25 0.025 0.03 0.03 0.08 0.025 0.025
Na
2
EDTA2H
2
O 45.4 45.4 45.4 37.26 37.26 186.0 37.25 3.35 37.26 37.26
Ferric Chloride 1
Ferrous Sulfate7H
2
O 33.8 33.8 33.8 27.8 27.8 139.0 27.85 25 2.5 27.85 27.85
Magnesium Sulfate, Anhydrous Magnesium Sulfate, Anhydrous 361.49 361.49 361.49 122.09 122.09 122.09 17.099 122.1 351.63 240.76 146.55 146.55
Manganese Chloride4H
2
O 1.81
Manganese SulfateH
2
O 33.5 33.5 33.5 10 10 132.0 1 0.0758 0.01 10 10
Molybdic Acid (Sodium Salt)2H
2
O 0.39 0.39 0.39 0.25 0.25 0.25 0.025 0.25 0.25
Molybdenum Trioxide 0.016
Nickel Chloride 0.03 0.03
Nickel Sulfate6H
2
O 0.005 0.005 0.005
Potassium Chloride 300 65 750 65 300 300
Potassium Iodide 0.75 0.75 0.75 0.8 0.01 0.01 0.75 0.75
Potassium Nitrate 2500 2500 1000 1000 80 606.6 1900 1900
Potassium Phosphate, Monobasic 265 265 265 300 170 170
Potassium Sulfate 1559 1559 1559
Sodium Nitrate 600
Sodium Phosphate Monobasic 150 150 90.0 108.75 16.5
Sodium Sulfate 200
Zinc Nitrate6H
2
O 17 17 17
Zinc Sulfate7H
2
O 2 2 3 0.3 1 1 0.22 2 2
p-Aminobenzoic Acid 0.2 0.02
L-Arginine (Free Base) 40
L-Ascorbic Acid 0.4 2
Asparagine 40
D-Biotin 0.00025 0.2 0.01
D-Calcium Pantothenate 0.4 1
Choline Chloride 0.2 1
Citric Acid (Free Acid) Anhydrous 40
Cyancobalamin, Vit B
12
0.02
Folic Acid 0.015 0.4
Fumaric Acid 40
L-Glutamine 60
Glycine (Free Base) 20 4
L(-)-Maleic Acid 40
L-Methionine 30.0
myo-Inositol 100 100 10 100
Nicotinamide 1
Nicotinic Acid (Free Acid) 1 0.5 0.1
L-Phenylalanine 20
PyridoxineHCI 1 0.5 0.1 1
Pyruvic Acid 20
Ribofavin 0.015 0.2
Sucrose 10,000 30,000
ThiamineHCI 10 0.5 1 1
L-Tryptophan 40
Vitamin A 0.01
Vitamin D
3
0.01
Grams of powder to prepare 1 liter 5.22 15.22 35.22 3.10 3.21 3.64 2.71 1.64 1.04 1.63 3.65 3.9
pH0.5 at RT 4.3 4.0 4.1 4.0 4.0 NS 3.9 4.9 4.7 4.5 4.0 4.4
NS = No Specifcation Established
rev 11/2005 rev 11/2005
5
Product
Number
Product Description Product Notes
Package
Size
L689 LINSMAIER & SKOOG BASAL MEDIUM
This medium may be sold as Murashige & Skoog Medium with Minimal Organics
(MSMO) by some media suppliers.
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
Skoog (1965).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
L477 LINSMAIER & SKOOG MODIFIED BASAL MEDIUM
pH Adjusted and Buffered
This medium may be sold as Murashige & Skoog Medium with Minimal Organics
(MSMO) by some media suppliers.
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
Skoog (1965).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
L467 LINSMAIER & SKOOG BASAL MEDIUM
w/ 30 g/L SUCROSE & 7 g/L AGAR
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
Skoog (1965).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
L452 LINSMAIER & SKOOG MODIFIED BASAL MEDIUM
w/ 30 g/L SUCROSE & 7 g/L AGAR
pH Adjusted and Buffered
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
Skoog (1965).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
L154 LLOYD & McCOWN WOODY PLANT BASAL SALT MIXTURE
Contains the WPM macro- and micronutrients as described by Lloyd & McCown
(1981).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
L449 LLOYD & McCOWN WOODY PLANT BASAL MEDIUM w/ VITAMINS
Contains the WPM macro- and micronutrients, and vitamins as described by Lloyd &
McCown (1981).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
L444 LLOYD & McCOWN WOODY PLANT MICRONUTRIENT MIXTURE
Contains the WPM micronutrients described by Lloyd & McCown (1981).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M419 MG BASAL SALT MIXTURE
Modifed Murashige & Skoog/ Gamborg Basal Salt Mixture
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
MURASHIGE & SKOOG BASAL SALTS & MEDIA
See Murashige & Skoog-Based Basal Salts & Media Section (following this section)
N492 NB BASAL MEDIUM
Modifed Chu/ Gamborg Basal Medium
Contains the macronutrients as described by Chu (1975) and the micronutrients as
described by Gamborg, et al. (1968).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
N613 NITSCH & NITSCH BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Nitsch & Nitsch (1969).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
N616 NITSCH & NITSCH BASAL MEDIUM w/ VITAMINS
Contains the macro- and micronutrients, and vitamins as described by Nitsch & Nitsch
(1969).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
N608 NITSCH & NITSCH VITAMIN POWDER (1000x)
See Vitamin Section (following the MS-based media section)
N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)
See Vitamin Section (following the MS-based media section)
6
All components
expressed in mg/L
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o
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W
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d
y

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t

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a
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d
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m
L
l
o
y
d

&

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c
C
o
w
n

W
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d
y

P
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a
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t

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o
n
u
t
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t

M
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t
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M
G

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l

S
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M
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e

(
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/
G
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b
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g

B
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a
l

S
a
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t

M
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)
N
B

B
a
s
a
l

M
e
d
i
u
m
N
i
t
s
c
h

&

N
i
t
s
c
h

B
a
s
a
l

S
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l
t

M
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e
N
i
t
s
c
h

&

N
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c
h

B
a
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a
l

M
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d
i
u
m
COMPONENT L689 L477 L467 L452 L154 L449 L444 M419 N492 N613 N616
Ammonium Nitrate 1650 1650 1650 1650 400 400 720 720
Ammonium Sulfate 33.5 463
Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 6.2 2.3 3 10 10
Calcium Chloride, Anhydrous 332.2 332.2 332.2 332.2 72.5 72.5 72.5 111.36 125.33 166 166
Calcium Nitrate 386 386
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.025 0.0125 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.025 0.25 0.25 0.25 0.0125 0.025 0.025 0.025
Na
2
EDTA2H
2
O 37.26 37.26 37.26 37.26 37.3 37.3 37.3 18.64 37.26 37.26 37.26
Ferrous Sulfate7H
2
O 27.8 27.8 27.8 27.8 27.85 27.85 27.85 13.9 27.8 27.8 27.8
Magnesium Sulfate, Anhydrous 180.7 180.7 180.7 180.7 180.7 180.7 180.7 75.7 90.37 90.372 90.372
Manganese SulfateH
2
O 16.9 16.9 16.9 16.9 22.3 22.3 22.3 6.7 10 18.9 18.9
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.125 0.25 0.25 0.25
Potassium Hydroxide 100 100
Potassium Iodide 0.83 0.83 0.83 0.83 0.4 0.75
Potassium Nitrate 1900 1900 1900 1900 1100 2830 950 950
Potassium Phosphate, Monobasic 170 170 170 170 170 170 170 42.5 400 68 68
Potassium Sulfate 990 990
Sodium Nitrate 437.8
Sodium Phosphate Monobasic 32.6
Zinc Sulfate7H
2
O 8.6 8.6 8.6 8.6 8.6 8.6 8.6 2.7 2 10 10
Agar 7000 7000
D-Biotin 0.05
Folic Acid 0.5
Glycine (Free Base) 2 2
MES (Free Acid) 1000 1000
myo-Inositol 100 100 100 100 100 100 100
Nicotinic Acid (Free Acid) 0.5 1 5
PyridoxineHCI 0.5 1 0.5
Sucrose 30,000 30,000
ThiamineHCI 0.4 0.4 0.4 0.4 1 10 0.5
Grams of powder to prepare 1 liter 4.43 5.53 41.43 42.53 2.30 2.41 0.53 1.88 4.10 2.10 2.21
pH0.5 at RT NS 6.2 NS NS NS NS 4.3 4.0 3.8 NS
NS = No Specifcation Established rev 11/2005
7
Plant Tissue Culture Basal Salts and Media (continued)
Product
Number
Product Description Product Notes
Package
Size
N479 NLN BASAL MEDIUM
Contains the macro- and micronutrients, and organic constituents as described by
Lichter (1982); without sucrose.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
P713 PARKER THOMPSON BASIC C FERN BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Hickok, et al. (1995).
Formulated for the growth of Ceratopteris richardii.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
Q673 QUOIRIN & LEPOIVRE BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Quoirin & Lepoivre (1977) and
Quoirin, et al. (1977).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
R756 ROSE MODIFIED INITIATION BASAL MEDIUM
Stage I
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
R757 ROSE MODIFIED MULTIPLICATION BASAL MEDIUM
Stage II
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
R758 ROSE MODIFIED ROOTING BASAL MEDIUM
Stage III
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
S816 SCHENK & HILDEBRANDT BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Schenk and Hildebrandt
(1972).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
S811 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM
Contains 10 g/L Sucrose; Without Vitamins
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
S808 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM
Contains 10 g/L sucrose, 1x vitamins, 1x micro- and 1x macronutrients.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
S806 SCHENK & HILDEBRANDT MODIFIED BASAL SALT MIXTURE
Without Calcium
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
S813 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM
Contains vitamins and 10g/L sucrose.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
S826 SCHENK & HILDEBRANDT VITAMIN POWDER (100x)
See Vitamin Section (following the MS-based media section)
8
All components
expressed in mg/L
N
L
N

B
a
s
a
l

M
e
d
i
u
m
P
a
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k
e
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-
T
h
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m
p
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n

B
a
s
i
c

C

F
e
r
n

B
a
s
a
l

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Q
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&

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M
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e
S
c
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n
k

&

H
i
l
d
e
b
r
a
n
d
t

M
o
d
i
f

e
d

B
a
s
a
l

M
e
d
i
u
m
S
c
h
e
n
k

&

H
i
l
d
e
b
r
a
n
d
t

M
o
d
i
f

e
d

B
a
s
a
l

M
e
d
i
u
m
S
c
h
e
n
k

&

H
i
l
d
e
b
r
a
n
d
t

M
o
d
i
f

e
d

B
a
s
a
l

S
a
l
t

M
i
x
t
u
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e
S
c
h
e
n
k

&

H
i
l
d
e
b
r
a
n
d
t

M
o
d
i
f

e
d

B
a
s
a
l

M
e
d
i
u
m
COMPONENT N479 P713 Q673 R756 R757 R758 S816 S811 S808 S806 S813
Ammonium Molybdate 0.037
Ammonium Nitrate 125 400 1650 1650 412.5
Ammonium Phosphate,
Monobasic
300 300 150 300 300
Boric Acid 10 1.86 6.2 6.2 6.2 1.55 5.0 5.0 2.5 5.0 5.0
Calcium Chloride, Anhydrous 19.628 333 333 83.25 151 151 75.5 151
Calcium Nitrate 347 833.77
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.025 0.006 0.1 0.1 0.05 0.1 0.1
Cupric Sulfate5H
2
O 0.025 0.37 0.025 0.025 0.025 0.006 0.2 0.2 0.1 0.2 0.2
Na
2
EDTA2H
2
O 37.3 37.3 20 20 10 20 20
Ferric Sodium EDTA 36.7 36.7 36.7 9.175
Ferrous Sulfate7H
2
O 27.8 27.8 15 15 7.5 15 15
Magnesium Sulfate, Anhydrous 61 58.565 175.79 181 181 45.25 195.4 195.4 97.7 195.4 195.4
Manganese SulfateH
2
O 18.95 0.25 0.76 16.9 16.9 4.225 10 10 5 10 10
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.25 0.063 0.1 0.1 0.05 0.1 0.1
Potassium Iodide 0.08 0.83 0.83 0.208 1.0 1.0 0.5 1.0 1.0
Potassium Nitrate 125 1800 1900 1900 475 2500 2500 1250 2500 2500
Potassium Phosphate, Monobasic 125 500 270 170 170 42.5
Zinc Sulfate7H
2
O 10 0.52 8.6 8.6 8.6 2.15 1.0 1.0 0.5 1.0 1.0
L-Ascorbic Acid 50 50
6-Benzylaminopurine (BA) 2.0 3.0
D-Biotin 0.05
Citric Acid (Free Acid) Anhydrous 50 50
Folic Acid 0.5
L-Glutamine 800
Glutathion 30
Glycine (Free Base) 2.0 2.0 2.0 2.0
Indole-3-acetic Acid 0.30 0.30
myo-Inositol 100 100 100 100 500 1000
-Naphthaleneacetic Acid 0.03
Nicotinic Acid (Free Acid) 5.0 0.5 0.5 0.5 2.5 5.0
PyridoxineHCI 0.5 0.5 0.5 0.5 0.25 0.5
L-Serine 100
Sucrose 10000 10000 10000
ThiamineHCI 0.5 0.4 0.4 0.4 2.5 5.0
Grams of powder to prepare 1 liter 1.93 0.77 3.56 4.51 4.51 1.18 3.20 13.20 12.10 3.05 14.21
pH0.5 at RT 4.5 3.8 3.9 3.5 3.5 5.0 4.2 4.3 4.5 4.3 4.3
NS = No Specifcation Established
rev 11/2005
9
Plant Tissue Culture Basal Salts and Media (continued)
Product
Number
Product Description Product Notes
Package
Size
T853 TM4G BASAL SALT MIXTURE
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
T868 TM4G BASAL MEDIUM
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
T856 TOBACCO MODIFIED CALLUS INITIATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
T864 TOBACCO MODIFIED SHOOT MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
T867 TOBACCO MODIFIED SHOOT & ROOT BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
T861 TOBACCO MODIFIED ROOT INITIATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
W863 WESTVACO WV3 BASAL MEDIUM
Contains the macro- and micronutrients, and vitamins as described by Coke (1996b).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
W865 WESTVACO WV5 BASAL MEDIUM
Contains the macro- and micronutrients, and vitamins as described by Coke (1996a).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
W898 WHITE BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by White (1963).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
Murashige & Skoog Basal Salts and Media following this section.
10
All components expressed
in mg/L
T
M
4
G

B
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t

M
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T
M
4
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o

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u
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T
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&

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T
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i
a
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n

B
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M
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d
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W
e
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v
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W
V
3

B
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a
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M
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d
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W
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W
V
5

B
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d
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W
h
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B
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S
a
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t

M
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x
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e
COMPONENT T853 T868 T856 T864 T867 T861 W863 W865 W898
Ammonium Nitrate 320 320 1650 1650 1650 1650 700
Ammonium Phosphate, Monobasic 230 230
Ammonium Sulfate 130 130
Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 31.0 31.0 1.5
Calcium Chloride, Anhydrous 113.25 113.25 333 333 333 333 452.88 452.88
Calcium Nitrate 208.5
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.025 0.025 0.025 0.25 0.25 0.001
Na
2
EDTA2H
2
O 18.65 18.65
Ferric Sodium EDTA 36.7 36.7 36.7 36.7 36.7 36.71
Ferric Sulfate 2.5
Ferrous Sulfate 13.9 13.9
Magnesium Sulfate, Anhydrous 122.12 122.12 181 181 181 181 903.79 903.79 351.62
Manganese SulfateH
2
O 16.9 16.9 16.9 16.9 16.9 16.9 15.16 15.16 5.31
Molybdenum Trioxide 0.001
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
Potassium Chloride 656.79 718.67 65.0
Potassium Iodide 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.75
Potassium Nitrate 1900 1900 1900 1900 1900 1900 910.06 1084.06 80.0
Potassium Phosphate, Monobasic 170 170 170 170 270 270
Sodium Phosphate Monobasic 16.5
Sodium Sulfate 200
Zinc Sulfate7H
2
O 9.2 9.2 8.6 8.6 8.6 8.6 8.6 8.6 3.0
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 1000 1000 1000 1000
Folic Acid 0.5
Glycine (Free Base) 2.5 2.0 2.0 2.0 2.0
Indole-3-acetic Acid 2.0 0.03 3.0
Kinetin 0.2 1.0 1.0
myo-Inositol 100 100 100 100 100 1000 1000
Nicotinic Acid (Free Acid) 5.0 0.5 0.5 0.5 0.5
PyridoxineHCI 0.5 0.5 0.5 0.5 0.5
ThiamineHCI 0.5 0.4 0.4 0.4 0.4 0.4 0.4
Grams of powder to prepare 1 liter 2.88 2.99 5.41 5.41 5.41 5.41 5.22 0.934
pH0.5 at RT NS NS 5.5 4.3 NS 5.0 NS NS 4.7
NS = No Specifcation Established rev 11/2005
11
Murashige & Skoog-Based Basal Salts
Product
Number
Product Description Product Notes
Package
Size
M524 MURASHIGE & SKOOG BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
See Product No. M519 for Murashige & Skoog Basal Medium w/ Vitamins
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M499 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Contains FeNaEDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M502 MURASHIGE & SKOOG MACRONUTRIENT SALT BASE
Contains the macronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M654 MURASHIGE & SKOOG MACRONUTRIENT STOCK SOLUTION (10x)
Contains the macronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M554 MURASHIGE & SKOOG MICRONUTRIENT SALT BASE
Contains the micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M529 MURASHIGE & SKOOG MICRONUTRIENT STOCK SOLUTION (10x)
Contains the micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M153 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Contains 1x macro- and 1x micronutrients as described by Murashige & Skoog
(1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M561 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Contains 1x Ammonium Nitrate and 1x Potassium Nitrate, with the remaining macro-
and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M290 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Contains 1x Ammonium Nitrate, 1x Potassium Nitrate, and 1x Calcium Chloride, with
the remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M571 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Without Ammonium Nitrate. Contains the remaining macro- and micronutrients as
described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M531 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Without Nitrogen. Contains the macro- and micronutrients as described by Murashige
& Skoog (1962), modifed by eliminating NH
4
NO
3
and KNO
3
.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M407 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Without Nitrogen, Phosphorous, and Potassium
Contains the remaining macro- and micronutrients as described by Murashige & Skoog
(1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
12
All components
expressed in mg/L
M
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COMPONENT M524 M499 M502 M654 M554 M529 M153 M561 M290 M571 M531 M407
Ammonium Nitrate 1650 1650 1650 16500 825 825 825
Boric Acid 6.2 6.2 6.2 62 3.1 6.2 6.2 6.2 6.2 6.2
Calcium Chloride, Anhydrous 332.2 333 332.2 3322 166.1 332.2 166.1 332.2 332.2 332.2
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.25 0.0125 0.025 0.025 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.25 0.0125 0.025 0.025 0.025 0.025 0.025
Na
2
EDTA2H
2
O 37.26 37.26 373 18.63 37.26 37.26 37.26 37.26 37.26
Ferric Sodium EDTA 36.7
Ferrous Sulfate7H
2
O 27.8 27.8 278 13.9 27.8 27.8 27.8 27.8 27.8
Magnesium Sulfate, Anhydrous 180.7 181 180.7 1810 90.35 180.7 180.7 180.7 180.7 180.7
Manganese SulfateH
2
O 16.9 16.9 16.9 169 8.45 16.9 16.9 16.9 16.9 16.9
Molybdic Acid (Sodium
Salt)2H
2
O
0.25 0.25 0.25 2.5 0.125 0.25 0.25 0.25 0.25 0.25
Potassium Iodide 0.83 0.83 0.83 8.30 0.415 0.83 0.83 0.83 0.83 0.83
Potassium Nitrate 1900 1900 1900 19000 950 950 950 1900
Potassium Phosphate,
Monobasic
170 170 170 1700 85 170 170 170 170
Zinc Sulfate7H
2
O 8.6 8.6 8.6 86 4.3 8.6 8.6 8.6 8.6 8.6
Grams of powder to prepare
1 liter
4.33 4.30 4.23 N/A 0.10 NA 2.17 2.56 2.39 2.68 0.78 0.61
pH0.5 at RT 3.9 NS NS 4.3 4.3 3.2 NS 4.3 NS NS 4.3 4.3
NS = No Specifcation Established Rev. 11/2005
13
Murashige & Skoog-Based Media
Product
Number
Product Description Product Notes
Package
Size
M519 MURASHIGE & SKOOG BASAL MEDIUM w/ VITAMINS
With the macro- and micronutrients, and vitamins as described by Murashige & Skoog
(1962).
Plant Tissue Culture Tested
Contains the basal salts of Product No. M524
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M541 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM
Without Potassium Phosphate; contains FeNaEDTA in place of Ferrous Sulfate and
Disodium EDTA. Contains the remaining macro- and micronutrients, and modifed vitamins
as described by Murashige & Skoog (1962). Also contains (mg/L): 300 Sodium Phosphate
Monobasic, 150 Adenine Hemisulfate, and 1000 Casein Hydrolysate.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M404 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM w/ GAMBORG VITAMINS
With the macro- and micronutrients as described by Murashige & Skoog (1962), and
vitamins as described by Gamborg, et al. (1966).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M401 MURASHIGE & SKOOG MODIFIED MEDIUM
Contains the macro- and micronutrients as described by Murashige & Skoog (1962);
modifed with the addition of (mg/L): 1.0 BA and 0.1 NAA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M701 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM
This medium was formerly listed as M506.
This medium may be sold as Murashige Begonia Multiplication Medium by some media
suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog
(1962); modifed with the addition of (mg/L): 10 2iP and 30 Adenine Hemisulfate.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M702 MURASHIGE & SKOOG MODIFIED MEDIUM
Contains the macro- and micronutrients as described by Murashige & Skoog (1962);
modifed with the addition of (mg/L): 30 2iP and 80 Adenine Hemisulfate, and 0.3 IAA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M535 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM
Without Nicotinic Acid, PyridoxineHCl, and Glycine
Contains the macro- and micronutrients as described by Murashige & Skoog (1962);
modifed with the addition of (mg/L) 0.4 ThiamineHCl, 100 myo-Inositol, and 80 Adenine
Hemisulfate.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
MURASHIGE MEDIUM WITH MINIMAL ORGANICS (MSMO)
See L689 & L477 Linsmaier & Skoog Basal Medium
M536 MURASHIGE MODIFIED MULTIPLICATION BASAL MEDIUM
Contains the macro- and micronutrients, and vitamins as described by Murashige & Skoog
(1962); modifed with the addition of (mg/L): 2.0 2iP, 80 Adenine Hemisulfate, and 170
Sodium Phosphate.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M555 MURASHIGE & SKOOG MODIFIED MULTIPLICATION MEDIUM
This medium may be sold as Murashige & Skoog Shoot Multiplication Medium C by some
media suppliers. Contains the macro- and micronutrients as described by Murashige &
Skoog (1962); modifed with the addition of (mg/L): 1.0 Kinetin, 80 Adenine Hemisulfate,
and 148 Sodium Phosphate Monobasic.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M527 MURASHIGE MODIFIED MULTIPLICATION BASAL MEDIUM
This medium is sold as Murashige Shoot Tip Rooting Medium by some media suppliers.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and
vitamins as described by Linsmaier & Skoog (1965); modifed with the addition of (mg/L):
0.3 IAA, 1.0 Kinetin, and FeNaEDTA.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M491 MURASHIGE MODIFIED SHOOT MULTIPLICATION BASAL MEDIUM
This medium is sold as MS Shoot Multiplication Medium A by some media suppliers.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and
vitamins as described by Linsmaier & Skoog (1965); modifed with the addition of (mg/L):
170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30 2iP, and 0.3 IAA.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
MURASHIGE & SKOOG VITAMIN POWDERS & SOLUTIONS
See Vitamin Section (following this section)
14
All components
expressed in mg/L
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COMPONENT M519 M541 M404 M401 M701 M702 M535 M536 M555 M527 M491
Ammonium Nitrate 1650 1650 1650 1650 1650 1650 1650 1650 1650 1650 1650
Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2
Calcium Chloride, Anhydrous 332.2 332.2 332.2 332.2 333 332.2 332.2 332.2 332.2 333 333
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025
Na
2
EDTA2H
2
O 37.26 37.26 37.26 37.26 37.26 37.26 37.26
Ferric Sodium EDTA 36.7 36.7 36.7 36.7
Ferrous Sulfate7H
2
O 27.8 27.8 27.8 27.8 27.8 27.8 27.8
Magnesium Sulfate, Anhydrous 180.7 180.7 180.7 180.7 181 180.7 180.7 180.7 180.7 181 181
Manganese SulfateH
2
O 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
Potassium Iodide 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83
Potassium Nitrate 1900 1900 1900 1900 1900 1900 1900 1900 1900 1900 1900
Potassium Phosphate, Monobasic 170 170 170 170 170 170 170 170 170 170
Sodium Phosphate Monobasic 300 148 170 148 170
Zinc Sulfate7H
2
O 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6
Adenine Hemisulfate 150 30 80 80 80 80 80
6-Benzylaminopurine (BA) 1
Casein, Enzymatic Hydrolysate 1000
Glycine (Free Base) 2 2 2 2.0
6-,-Dimethylallylaminopurine (2iP) 10 30 30
Indole-3-acetic Acid 1 0.3 0.3 0.3
Indole-3-butyric Acid
Kinetin 1 1
myo-Inositol 100 100 100 100 100 100 100 100 100 100 100
-Naphthaleneacetic Acid 0.1 0.1
Nicotinic Acid (Free Acid) 0.5 5 1 0.5 0.5
PyridoxineHCI 0.5 1 1 0.5 0.5
Sucrose 30000 30000 30000
ThiamineHCI 0.1 0.5 10 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4
Grams of powder to prepare 1 liter 4.43 5.69 4.44 34.44 4.44 34.69 4.51 4.68 34.66 4.41 4.68
pH0.5 at RT 3.9 4.0 4.0 4.0 3.9 3.8 4.0 3.5 4.4 NS NS
NS = No Specifcation Established Rev 11/2005
15
MS-Based Plant-Specifc Media
Product
Number
Product Description Product Notes
Package
Size
M517 MURASHIGE MODIFIED AFRICAN VIOLET/ GLOXINIA
MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M518 MURASHIGE MODIFIED AFRICAN VIOLET/ GLOXINIA
PRETRANSPLANT BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M550 MURASHIGE & SKOOG MODIFIED MEDIUM
Arabidopsis Culture Medium
Modifed with the addition of (mg/L): 2.0 2,4-D, and 0.05 Kinetin
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M506 MURASHIGE BEGONIA MULTIPLICATION MEDIUM
See Product No. M701 Murashige & Skoog Modifed Medium (same formulation)
M508 MURASHIGE MODIFIED FERN MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M509 MURASHIGE MODIFIED GERBERA MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M510 MURASHIGE MODIFIED GERBERA PRETRANSPLANT BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
H435 HOSTA INITIATION/ MULTIPLICATION MEDIUM
Stage I/II Medium
Modifed Murashige & Skoog (1962) with (mg/L): 2.0 BA, 160 Adenine Hemisulfate, 500
Casein Hydrolysate, and 0.5 NAA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
H436 HOSTA MULTIPLICATION MEDIUM
Stage II Medium
Modifed Murashige & Skoog (1962) with (mg/L): 0.1 BA, 160 Adenine Hemisulfate, 500
Casein Hydrolysate, and 0.5 NAA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
H437 HOSTA ROOTING MEDIUM
Stage III Medium
Modifed Murashige & Skoog (1962) with (mg/L): 0.1 BA, 500 Casein Hydrolysate, and 0.5
NAA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
16
All components
expressed in mg/L
M
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COMPONENT M517 M518 M550 M508 M509 M510 H435 H436 H437 M519
Ammonium Nitrate 1650 1650 1650 1650 1650 1650 1650 1650 1650 1650
Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2
Calcium Chloride, Anhydrous 333 333 332.2 333 333 333 332.2 332.2 332.2 332.2
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025
Na
2
EDTA2H
2
O 37.26 37.26 37.26 37.26 37.26
Ferric Sodium EDTA 36.7 36.7 36.7 36.7 36.7
Ferrous Sulfate7H
2
O 27.8 27.8 27.8 27.8 27.8
Magnesium Sulfate, Anhydrous 181 181 180.7 181 181 181 180.7 180.7 180.7 180.7
Manganese SulfateH
2
O 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
Potassium Iodide 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83
Potassium Nitrate 1900 1900 1900 1900 1900 1900 1900 1900 1900 1900
Potassium Phosphate, Monobasic 170 170 170 170 170 170 300 300 300 170
Sodium Phosphate Monobasic 170 255 85 85 170 170 170
Zinc Sulfate7H
2
O 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6
Adenine Hemisulfate 80 80 160 160
Agar 8000 8000 8000
6-Benzylaminopurine (BA) 2.0 0.1 0.1
Casein, Enzymatic Hydrolysate 500 500 500
Glycine (Free Base) 2.0 2.0 2.0
2,4-Dichlorophenoxyacetic Acid 2.0
Indole-3-acetic Acid 2.0 1.0 0.5 10
Indole-3-butyric Acid
Kinetin 2.0 0.05 2.0 10
MES (Free Acid)
myo-Inositol 100 100 100 100 100 100 100 100 100 100
a-Naphthaleneacetic Acid 0.1 0.5 0.5 0.5
Nicotinic Acid (Free Acid) 1.0 10 10 0.5
Peptone from Meat
PyridoxineHCI 1.0 1.0 1.0 0.5
Sucrose 20000 30000 30000 30000
ThiamineHCI 0.4 0.4 10 0.4 30 30 0.4 0.4 0.4 0.1
L-Tyrosine 100 100
Grams of powder to prepare 1 liter 4.66 4.4 24.44 4.66 4.72 4.64 43.40 43.39 43.23 4.43
pH0.5 at RT 4.0 4.8 4.0 4.5 NS 5.9 4.3 4.3 4.3 3.9
NS = No Specifcation Established Rev 11/2005
17
MS-Based Plant-Specifc Media (continued)
Product
Number
Product Description Product Notes
Package
Size
M511 MURASHIGE MODIFIED KALANCHOE MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M512 MURASHIGE MODIFIED KALANCHOE PRETRANSPLANT BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M513 MURASHIGE MODIFIED LILY MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M462 MUSA (BANANA) MULTIPLICATION MEDIUM
IITA Formulation as described by Vuylsteke (1998).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
M516 MURASHIGE & SKOOG MODIFIED BC POTATO BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
1 L
10 L
50 L
All components
expressed in mg/L
M
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COMPONENT M511 M512 M513 M462 M516
Ammonium Nitrate 1650 1650 1650 1650 1650
Boric Acid 6.2 6.2 6.2 6.2 6.2
Calcium Chloride, Anhydrous 333 333 333 332.2 333
Cobalt Chloride6H
2
O 0.025 0.025 0.025 0.025 0.025
Cupric Sulfate5H
2
O 0.025 0.025 0.025 0.025 0.025
Na
2
EDTA2H
2
O 37.25
Ferric Sodium EDTA 36.7 36.7 36.7 36.7
Ferrous Sulfate7H
2
O 27.85
Magnesium Sulfate, Anhydrous 181 181 181 180.74 181
Manganese SulfateH
2
O 16.9 16.9 16.9 16.9 16.9
Molybdic Acid (Sodium Salt)2H
2
O 0.25 0.25 0.25 0.25 0.25
Potassium Iodide 0.83 0.83 0.83 0.83 0.83
Potassium Nitrate 1900 1900 1900 1900 1900
Potassium Phosphate, Monobasic 170 170 170 170 170
Zinc Sulfate7H
2
O 8.6 8.6 8.6 8.6 8.6
L-Ascorbic Acid 20
6-Benzylaminopurine (BA) 4.5
Gelrite 2000
D-Glucose 2.0
Glycine (Free Base) 2.0 2.0
6-,-Dimethylallylaminopurine (2iP) 3.0
Indole-3-acetic Acid 3.0 0.175
Kinetin 0.04
myo-Inositol 100 100 100 100
-Naphthaleneacetic Acid 0.03
Nicotinic Acid (Free Acid) 0.5 0.5
PyridoxineHCI 0.5 0.5
Sucrose 30,000
ThiamineHCI 0.4 0.4 0.4 0.4 0.4
Grams of powder to prepare 1 liter 4.41 4.41 4.4 36.36 4.41
pH0.5 at RT 4.8 4.8 4.8 4.3 NS
NS = No Specifcation Established
18
Vitamins
Product
Number
Product Description Product Notes
Package
Size
C149 CHU N6 VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Chu (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
E330 ERIKSSON VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Eriksson (1965).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
G249 GAMBORG VITAMIN POWDER (1000x)
Contains the vitamins as described by Gamborg, et al. (1968).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
G219 GAMBORG VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Gamborg, et al. (1968).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
K421 KAO & MICHAYLUK VITAMIN SOLUTION (100x)
Contains the vitamins described by Kao & Michayluk (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
500 mL
1 L
M533 MURASHIGE & SKOOG VITAMIN POWDER (1000x)
Contains the vitamins as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
250 mL
M553 MURASHIGE & SKOOG VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
M547 MURASHIGE & SKOOG MODIFIED VITAMIN POWDER (1000x)
Murashige & Skoog (1962) vitamins modifed with additional ThiamineHCl.
Plant Tissue Culture Tested
100 mL
250 mL
M557 MURASHIGE & SKOOG MODIFIED VITAMIN SOLUTION (1000x)
Murashige & Skoog (1962) vitamins modifed with additional ThiamineHCl.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
N608 NITSCH & NITSCH VITAMIN POWDER (1000x)
Contains the vitamins as described by Nitsch & Nitsch (1969).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
250 mL
N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Nitsch & Nitsch (1969).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
S826 SCHENK & HILDEBRANDT VITAMIN POWDER (100x)
Contains the vitamins as described by Schenk & Hildebrandt (1972).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6 C
Water
100 mL
1 L
All components
expressed in mg/L
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COMPONENT C149 E330 G249 G219 K421 M533 M553 M547 M557 N608 N603 S826
p-Aminobenzoic Acid 2.0
L-Ascorbic Acid 200
D-Biotin 1.0 50 50
D-Calcium Pantothenate 100
Choline Chloride 100
Cyancobalamin, Vit B
12
2.0
Folic Acid 40.0.0 500 500
Glycine (Free Base) 2000 2000 2000 2000 2000 2000 2000 2000
myo-Inositol 100000 100000 10000 100000 100000 100000 100000 100000 100000 100000
Nicotinamide 100
Nicotinic Acid (Free Acid) 500 500 1000 1000 500 500 500 500 5000 5000 500
PyridoxineHCl 500 500 1000 1000 100 500 500 500 500 500 500 50.0
Ribofavin 20.0
ThiamineHCI 1000 500 10000 10000 100 100 100 1000 1000 500 500 500
Vitamin A 1.0
Grams of powder to prepare 1 L N/A N/A 112 N/A N/A 103.1 N/A 104 N/A 108.55 N/A 101.05
Rev 11/2005