(1)
4
2 2 2
k
red OX
E O E H O + +
(2)
5
k
red OX OX red
E Med E Med + +
(3)
We assume that the concentrations of all reactants and
enzyme intermediates remain constant for all time.
Also the concentration of total active enzyme [ ]
t
E and
the reactants in the bulk electrode remain constant. We
can consider that the diffusion of the reactants can be
described by Ficks second law and the enzymes are
assumed to be uniformly dispersed throughout the
matrix. The enzyme activity is not a function of
position. The reaction/diffusion equations correspond-
ing to the concentrations of substrate, oxygen and
oxidized mediator within a matrixcan be expressed
as[14]
| |
| |
| |
| |
1
1
2
2
3
2
1
OX s
s t
o m
Med d S O
D k E
S dy
(
| |
(
= + + +
|
|
(
\ .
(4)
| | | | | |
| |
| |
| | | |
| |
2
2
2
1
1
2
3
/ /
/
1
OX o OX m
m
OX m
OX s
t
o m
d Med O Med
D
Med dy
Med O
k E
S
| | +
|
|
\ .
(
| |
(
= + + +
|
|
(
\ .
(5)
| | | | | |
| |
| |
| | | |
| |
2
2 2
2
2
1
1
2
3
/ /
/
1
o OX m
o
o
OX s
t
o m
O Med d O
D
O dy
Med O
k E
S
| | +
|
|
\ .
(
| |
(
= + + +
|
|
(
\ .
(6)
where [ ] S , | |
OX
Med and | |
2
O are the concentrations
of substrate, oxidized mediator and oxygen and y is
distance from the electrode.
s
D ,
m
D and
o
D are the
diffusion coefficients of substrate, oxidized mediator
and oxygen. [ ]
t
E Denotes the concentration of the
total active enzyme in the matrix and ( )
2 3 1
/
s
k k k = + ,
3 4
/
o
k k = &
3 5
/
m
k k = represent reaction constants
of substrate, oxygen and oxidized mediator
respectively. The boundary conditions are given by
When y d = ,
| | | | | |
2 2 2 o
b
O O K O
= = , | | | | | |
s
b b
S S K S = = ,
| | | | | |
OX OX m OX
b
Med Med K Med
= =
(7)
when 0, y =
| | | |
OX OX
b
Med Med = | | | |
2
/ / 0 d O dy d S dy = =
(8)
Here | |
b
S , | |
2
b
O | |
OX
b
Med and| | S
, | |
2
O
, | |
OX
Med
are the bulk concentrations of substrate, oxygen and
oxidized mediator species.
o
K ,
s
K and
m
K are the
www.seipub.org/fs Frontiers in Sensors (FS) Volume 2, 2014
34
partition coefficients for oxygen, substrate and
oxidized mediator respectively. Equation (4) can be
represented in the normalized form using the
following normalized parameters.
| |
| |
s
b
S
F
S
= ;
| |
| |
2
2
o
b
O
F
O
= ;
| |
| |
OX
m
OX
b
Med
F
Med
= ;
y
x
d
= ; (9)
| |
b
s
s
S
B
=
| |
2
b
o
o
O
B
= ;
| |
OX
b
m
m
Med
B
= ; (10)
| |
| |
2
3 2 t
s
s
b
d k E
D S
= ;
| |
| |
2
3 2
2
t
o
o
b
d k E
D O
= ;
| |
| |
2
3 2 t
m
m OX
b
d k E
D Med
= (11)
where
s
F ,
o
F and
m
F represent the normalized
concentrations of substrate, oxygen and oxidized
mediator and
s
B ,
o
B and
m
B are the corresponding
normalized surface concentrations. The surface
concentration is the ratio of the bulk concentration and
the reaction constants.
2
s
,
2
o
and
2
m
denote the
thiele moduli of substrate, oxygen and oxidized
mediator, respectively. Thiele modulus
2
represents
the ratio of the characteristic time of the enzymatic
reaction to that of substrate diffusion and dis the
thickness of the enzyme layer. The systems of three
non-linear reaction/diffusion equations in normalized
form are
2
2
2
1
1 1
1
s
s
o o m m s s
d F
dx
F B F B F B
| |
|
|
=
|
+ +
|
+
\ .
(12)
2
2
2
1
1 1
1
o o o
o
o o m m
o o m m s s
d F F B
F B F B dx
F B F B F B
| |
|
| |
|
=
|
+ |
\ .
+ +
|
+
\ .
(13)
2
2
2
1
1 1
1
m m m
m
o o m m
o o m m s s
d F F B
F B F B dx
F B F B F B
| |
|
| |
|
=
|
+ |
\ .
+ +
|
+
\ .
(14)
The boundary conditions become
1 1
s o m
F F F at x = = = =
(15)
1
m
F = , 0 0
o s
dF dF
at x
dx dx
= = =
(16)
The normalized current
OX
J is given by,
0
m
OX
x
dF
J
dx
=
| |
=
|
\ .
(17)
Anal yt i c al Ex pr essi on of t he Nor mal i zed
Sur f ac e Conc ent r at i ons Usi ng t he New
Homot opy Per t ur bat i on Met hod
Linear and non-linear phenomena are of fundamental
importance in various fields of science and
engineering. Most models of real life problems are
still very difficult to solve. Therefore, approximate
analytical solutions such as Homotopy perturbation
method (HPM) [Gohri et. al (2007), Ozis et. al (2007),
Li et. al (2006), Mousa et. al (2008), He (1999 and 2003),
Ariel (2010), Ananthaswamy et. al (2012 and 2013)]
were introduced. This method is the most effective
and convenient ones for both linear and non-linear
equations. Perturbation method is based on assuming
a small parameter. The majority of non-linear
problems, especially those having strong non-linearity,
have no small parameters at all and the approximate
solutions obtained by the perturbation methods, in
most cases, are valid only for small values of the small
parameter. Generally, the perturbation solutions are
uniformly valid as long as a scientific system
parameter is small. However, we cannot rely fully on
the approximations, because there is no criterion on
which the small parameter should exists. Thus, it is
essential to check the validity of the approximations
numerically and/or experimentally. To overcome these
difficulties, HPM have been proposed recently.
Recently, many authors have applied the Homotopy
perturbation method (HPM) to solve the non-linear
boundary value problem in physics and engineering
sciences [Ghori et. al (2007), Ozis et. al (2007), Mousa
et. al (2008)]. Recently this method is also used to solve
some of the non-linear problem in physical sciences
[He 1999 and 2003 ]. This method is a combination of
Homotopy in topology and classic perturbation
techniques. Ji-Huan He used to solve the Lighthill
equation [Mousa et. al (2008)], the Diffusion equation
[He 1999] and the Blasius equation [He 2003]. The
HPM is unique in its applicability, accuracy and
efficiency. The HPM uses the imbedding parameter p
as a small parameter, and only a few iterations are
needed to search for an asymptotic solution. The
simple analytical expressions of the concentrations of
substrate, oxygen and oxidized mediator are as
follows:
( ) ( )
2
2 1
1 1
2
s
k
F x x = +
(18)
( ) ( )
2
2 2
1 1
2
o
k
F x x = +
(19)
Frontiers in Sensors (FS) Volume 2, 2014 www.seipub.org/fs
35
( ) ( )
2
2 3
1
2
m
k
F x x x = +
(20)
where,
2 2
1
( )
( ) ( )
o m s
s
o m s s o m
B B B
k
B B B B B B
| | +
=
|
+ + + +
\ .
(21)
2 2
2
( ) ( )
o s
o
o m s s o m
B B
k
B B B B B B
| |
=
|
+ + + +
\ .
(22)
2 2
3
( ) ( )
m s
m
o m s s o m
B B
k
B B B B B B
| |
=
|
+ + + +
\ .
(23)
The corresponding normalized current using eqn. (20):
2
3
2
OX
k
J = (24)
where
2
3
k is defined by the eqn. (23).
Numer i c al Si mul at i on
The non-linear reaction-diffusion equations eqns. (12)-
(14) for the boundary conditions eqns. (15) and (16) are
also solved numerically. We have used the function
pdex4 in Scilab/Matlab numerical software to solve
numerically, the initial-boundary value problems for
parabolic-elliptic partial differential equations. This
numerical solution is compared with our simple
analytical equations in Figs. (2) - (6). A satisfactory
agreement is noted for various values of the thiele
moduli
2
s
,
2
o
and
2
m
and possible small values of
the dimensionless parameters
s
B ,
o
B and
m
B .
Resul t s and Di sc ussi ons
Figure.1 is the schematic model of an enzyme-
membrane electrode. Figure2 is the normalized
concentration of (a)substrate
s
F , normalized
concentration of (b)oxygen
o
F and normalized
concentration of (c) mediator
m
F versus the
normalized distance x. From Fig.2 (a), (b) and (c) it is
clear that when the thiele modulus
2 2 2
, and
s o m
increases, the corresponding normalized
concentrations of substrate ,
s
F oxygen
o
F , mediator
m
F decreases for some fixed values of the normalized
surface concentrations of the substrate
s
B , normalized
surface concentration of oxygen
o
B and normalized
surface concentration of the mediator
m
B respectively.
Figure3 is the normalized concentrations of
(a)substrate
s
F , (b)oxygen
o
F and (c) mediator
m
F
versus the normalized distance x. From Fig. 3 ((a)-(c))
we infer that when the normalized parameter
s
B
increases, the corresponding normalized concentration
of substrate ,
s
F oxygen
o
F and mediator
m
F decreases
for some fixed values of
o
B ,
m
B when
2
1,
s
=
2
1
o
=
and
2
1
m
= .
Figure4 is the normalized concentrations of
(a)substrate
s
F , (b)oxygen
o
F and (c) mediator
m
F
versus the normalized distance x. From Fig. 4 (a)-(b) it
is clear that when the normalized parameter
o
B
increases, the corresponding normalized concentrations
of substrate ,
s
F oxygen
o
F decreases for some fixed
values of
s
B ,
m
B when
2
1,
s
=
2
1
o
= . Figure. 4 (c) it is
clear that when the normalized parameter
s
B
increases, the corresponding normalized concentrations
of mediator
m
F increases for some fixed values of
s
B ,
m
B and
2
1
m
= .
Figure.5 is the normalized concentrations of
(a)substrate
s
F , (b)oxygen
o
F and (c) mediator
m
F
versus the normalized distance x. From Figs. 5 (a) and
(c) it is clear that when the normalized parameter
m
B
increases, the corresponding normalized concentrations
of substrate
s
F and mediator
m
F decreases for some
fixed values of
s
B ,
o
B when
2
1
s
= and
2
1.
m
= Figure.
5 (b) it is clear that when the normalized parameter
m
B
increases, the corresponding normalized
concentrations of oxygen
o
F increases for some fixed
values of
s
B ,
o
B and
2
1
o
= .
Figure.6 is the normalized concentrations of the
substrate
s
, F oxygen
o
F and the mediator
m
F versus the
dimensionless distance x . From this figure, we note
that the substrate, oxygen and mediator increasesfor
some fixed values of the parameters , ,
s o m
B B B and
2 2 2
1
s o m
= = =
Figure.7 is the variation of normalized
current
OX
J with (a) normalized thiele modulus
2
m
for
the mediator (b) normalized surface concentration of
the substrate
s
B (c) normalized surface concentration
of oxygen
o
B and (d) normalized surface concentration
of mediator
m
B . From these Figs. 7 (a), (c) and (d) the
normalized current decreases and (b) increases for
some fixed values.
FIGURE 1: GEOMETRY OF THE PROBLEM
www.seipub.org/fs Frontiers in Sensors (FS) Volume 2, 2014
36
(a)
(b)
(c)
FIGURE 2: NORMALIZED CONCENTRATIONS OF (A)
SUBSTRATE
s
F (EQN. (18)) (B) OXYGEN
o
F (EQN.(19)) AND
(C)MEDIATOR
m
F (EQN. (20)) COMPUTED FOR SOME FIXED
VALUES OF THE PARAMETERS 0.5 B
s
= , 0.05
o
B = , 0.1
r
B =
AND VARIOUS VALUES OF THIELE MODULUS
2
s
,
2
o
AND
2
m
.
(a)
(b)
(c)
FIGURE 3: NORMALIZED CONCENTRATIONS OF (A)
SUBSTRATE
s
F (EQN. (18)) (B) OXYGEN
o
F (EQN.(19)) AND
(C)MEDIATOR
m
F (EQN. (20)) COMPUTED FOR SOME FIXED
VALUES OF THE PARAMETERS 0.05
o
B = , 0.1
r
B = AND
2
1
s
=
AND VARIOUS VALUES OF NORMALIZED SURFACE
CONCENTRATION OF SUBSTRATE
S
B , WHEN (I)
S
0.001 B = (II)
S
0.005 B = (III)
S
0.01 B = (IV)
S
0.05 B = (V)
S
0.1 B = AND
(V) 0.5 B
s
=
Frontiers in Sensors (FS) Volume 2, 2014 www.seipub.org/fs
37
(a)
(b)
(c)
FIGURE 4: NORMALIZED CONCENTRATIONS OF (A)
SUBSTRATE
s
F (EQN. (18)) (B) OXYGEN
o
F (EQN.(19)) AND
(C)MEDIATOR
m
F (EQN. (20)) COMPUTED FOR SOME FIXED
VALUES OF THE PARAMETERS 0.5 B
s
= , AND
2
1
o
= AND
VARIOUS VALUES OF NORMALIZED SURFACE
CONCENTRATION OF OXYGEN WHEN, (I) 0.001
o
B = (II)
0.05
o
B = (III) 0.1
o
B = (IV) 0.5
o
B = (V) 1
o
B = AND (VI) 2
o
B = .
(a)
(b)
(c)
FIGURE 5: NORMALIZED CONCENTRATIONS OF (A)
SUBSTRATE
s
F (EQN. (18)) (B) OXYGEN
o
F (EQN.(19)) AND
(C)MEDIATOR
m
F (EQN. (20)) COMPUTED FOR SOME FIXED
VALUES OF THE PARAMETERS 0.5 B
s
= , 0.05
o
B = AND
2
1
m
=
AND VARIOUS VALUES OF NORMALIZED SURFACE
CONCENTRATION OF MEDIATOR(II) 0.005
m
B = (III) 0.01
m
B =
(IV) 0.05
m
B = (V) 0.1
m
B = (VI) 0.2
m
B = AND (VI) 0.5
m
B = .
www.seipub.org/fs Frontiers in Sensors (FS) Volume 2, 2014
38
FIGURE6: NORMALIZED CONCENTRATIONS OF SUBSTRATE
s
F (EQN. (18)) VERSUS THE NORMALIZED DISTANCE x ,
OXYGEN
o
F (EQN. (19))VERSUS THE NORMALIZED DISTANCE
x WHEN 0.5 B
s
= , 0.05
o
B = , 0.1
m
B = AND
2
1
o
= AND
MEDIATOR
m
F (EQN.(20)) VERSUS THE NORMALIZED
DISTANCE x WHEN 0.5 B
s
= , 0.05
o
B = , 0.1
m
B = AND
2
1
m
= .
(a)
(b)
(c)
(d)
FIGURE7: VARIATION OF NORMALIZED CURRENT
OX
J WITH
(A) NORMALIZED THIELE MODULUS FOR THE MEDIATOR (B)
NORMALIZED SURFACE CONCENTRATION OF THE
SUBSTRATE
s
B (C) NORMALIZED SURFACE CONCENTRATION
OF OXYGEN
o
B AND (D) NORMALIZED SURFACE
CONCENTRATION OF MEDIATOR
m
B .
Conc l usi ons
The system of non-linear reaction diffusion equations
of the model has been solved analytically. The
approximate analytical expressions for the
concentrations of substrate, oxygen, mediator and the
current at the enzyme-membrane electrode geometry
are obtained using the new Homotopy perturbation
method. This analytical result is useful for
improvingthe sensor design. The extension of the
procedure to the model with reduced mediator in the
bulk solution seems possible. These analytical
expressions can be used for the optimization of the
Frontiers in Sensors (FS) Volume 2, 2014 www.seipub.org/fs
39
thickness of the enzyme layer or Thiele modulus
which produces significant change in both the
magnitude of the current and the general behavior of
the system.
ACKNOWLEDGEMENT
The authors are thankful to the Secretary, the Principal
and the Head of the Department of Mathematics, The
Madura College, Madurai, India for their constant
encouragement.
Appendi x A: Basi c Conc ept of t he Homot opy
Per t ur bat i on Met hod
To explain this method, let us consider the following
function:
( ) ( ) 0, r
o
D u f r = (A.1)
with the boundary conditions of
( , ) 0, r
o
u
B u
n
(A.2)
where
o
D is a general differential operator,
o
B is a
boundary operator, ( ) f r is a known analytical
function and is the boundary of the domain . In
general, the operator
o
D can be divided into a linear
part L and a non-linear part N . The eqn.(A.1) can
therefore be written as
( ) ( ) ( ) 0 L u N u f r + = (A.3)
By the Homotopy technique, we construct a
Homotopy ( , ): [0,1] v r p that satisfies
0
( , ) (1 )[ ( ) ( )] [ ( ) ( )] 0
o
H v p p L v L u p D v f r = + = (A.4)
0 0
( , ) ( ) ( ) ( ) [ ( ) ( )] 0 H v p L v L u pL u p N v f r = + + = (A.5)
where p[0, 1] is an embedding parameter, and
0
u is
an initial approximation of the eqn.(A.1) that satisfies
the boundary conditions. From the eqns. (A.4) and
(A.5), we have
0
( ,0) ( ) ( ) 0 H v L v L u = = (A.6)
( ,1) ( ) ( ) 0
o
H v D v f r = = (A.7)
When p=0, the eqns.(A.4) and (A.5) become linear
equations. When p =1, they become non-linear
equations. The process of changing p from zero to
unity is that of
0
( ) ( ) 0 L v L u = to ( ) ( ) 0
o
D v f r = . We
first use the embedding parameter p as a small
parameter and assume that the solutions of the eqns.
(A.4) and (A.5) can be written as a power series in p :
2
0 1 2
... v v pv p v = + + + (A.8)
Setting 1 p = results in the approximate solution of
the eqn. (A.1):
0 1 2
1
lim ...
p
u v v v v
= = + + + (A.9)
This is the basic idea of the HPM.
Appendi x B: Anal yt i c al Sol ut i on of t he
Syst em of Non-l i near Equat i ons (12)-(16)
Usi ng New Homot opy Per t ur bat i on
Met hod [ 16-27]
In this Appendix, we indicate how the eqns. (18)-(20)
in this paper is derived. To find the solution of the
eqns.(12) - (14), we construct the new Homotopy as
follows:
( )
( )
( )
( )
( )
( )
2 2
2
2
2
2
(1) (1) (1)
(1 )
(1) (1) (1) (1)
(1) (1)
0
s o o m m s s s
o o m m s s s s
o o m m
o o m m s s s
s
o o m m s s s s
o o m m
F B F B F B d F
p
F B F B F B F B dx
F B F B
F B F B F B d F
p
F B F B F B F B dx
F B F B
( | |
( |
+ ( |
( |
+ +
( |
( |
+ +
\ .
( | |
( |
+ ( |
+ =
( |
+ +
( |
( |
+ +
\ .
(B.1)
( )
( )
( )
2 2
2
2
2
2
( )
(1 )
( ) ( )
0
s s o m s
o m s s o m
s
o o m m s s
s
o o m m s s s s
o o m m
d F B B B
p
B B B B B B dx
d F
dx
p
F B F B F B
F B F B F B F B
F B F B
(
+
(
+ + + +
(
(
(
(
| |
(
|
+ =
( +
|
(
|
+ +
(
|
(
|
+ +
\ .
(B.2)
( )
( )
( )
( )
2
2
2
2
2
2
(1 )
(1) (1)
(1) (1) (1) (1)
(1) (1)
0
o
o o o s s
o o m m s s s s
o o m m
o o o s s
o
o o m m s s s s
o o m m
d F
dx
p
F B F B
F B F B F B F B
F B F B
d F F B F B
p
F B F B F B F B dx
F B F B
(
(
(
(
| |
(
|
(
|
(
|
+ +
(
|
(
|
+ +
\ .
( | |
( |
( |
+ =
( |
+ +
( |
( |
+ +
\ .
(B.3)
www.seipub.org/fs Frontiers in Sensors (FS) Volume 2, 2014
40
( )
( )
2 2
2
2
2
2
(1 )
( ) ( )
0
o o o s
o m s s o m
o
o o s s
o
o o m m s s s s
o o m m
d F B B
p
B B B B B B dx
d F
dx
p
F B F B
F B F B F B F B
F B F B
(
(
+ + + +
(
(
(
(
| |
(
|
+ =
(
|
(
|
+ +
(
|
(
|
+ +
\ .
(B.4)
( )
( )
( )
( )
2
2
2
2
2
2
(1 )
(1) (1)
(1) (1) (1) (1)
(1) (1)
0
m
m m m s s
o o m m s s s s
o o m m
m
m m s s
m
o o m m s s s s
o o m m
d F
dx
p
F B F B
F B F B F B F B
F B F B
d F
dx
p
F B F B
F B F B F B F B
F B F B
(
(
(
(
| |
(
|
(
|
(
|
+ +
(
|
(
|
+ +
\ .
(
(
(
(
| |
(
|
+ =
(
|
(
|
+ +
(
|
(
|
+ +
\ .
(B.5)
( )
( )
2 2
2
2
2
2
(1 )
( ) ( )
0
m m m s
o m s s o m
m
m m s s
m
o o m m s s s s
o o m m
d F B B
p
B B B B B B dx
d F
dx
p
F B F B
F B F B F B F B
F B F B
(
(
+ + + +
(
(
(
(
| |
(
|
+ =
(
|
(
|
+ +
(
|
(
|
+ +
\ .
(B.6)
The analytical solution of the eqn.(B.2) is
0 1 2
2
..........
s s s s
F F pF p F = + + + (B.7)
Similarly the analytical solutions of the eqns. (B.4) and
(B.6) are
1
0
2
2
..........
o
o o o
F F pF p F = + + + (B.8)
0
2
1 2
..........
m m m m
F F pF p F = + + +
(B.9)
Substituting the eqn.(B.7)-(B.9)into an eqn.(B.2) we get
0 1 2
2 2
2
2
( ...)
(1 )
( )
( ) ( )
s s s
s o m s
o m s s o m
d F pF p F
dx
p
B B B
B B B B B B
(
+ + +
(
(
(
+
(
+ + + + (
0 1 2
1 2 3
0 1 2
0 1 2
1 2 3
0 1 2
0 1 2
0 1 2
2 2
2
2
2
2
2
2
2
2
2
( ...)
( ...)
( ...)
( ...)
( ...)
( ...)
( ...)
( ..
s s s
o o o o
m m m m
s s s s
s
o o o o
m m m m
s s s s
s s s
d F pF p F
dx
F pF p F B
F pF p F B
p
F pF p F B
F pF p F B
F pF p F B
F pF p F B
F pF p F
+ + +
| |
+ + +
|
|
+ + + +
\ .
+
+ + +
| |
+ + +
|
|
+ + + +
\ .
+ + +
+ + + +
1 2 3
0 1 2
2
2
0
.)
( ...)
( ...)
s
o o o o
m m m m
B
F pF p F B
F pF p F B
(
(
(
(
| |
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
= (
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
| |
+ + +
(
|
|
+
(
|
|
+ + + +
(
\ . \ .
(B.10)
Substituting the eqns. (B.7)-(B.9) into an eqn.(B.4) we get
0 1 2
0 1 2
0 1 2
0 1 2
1 2 3
0 1 2
2 2
2
2
2 2
2
2
2
2
2
2
( ...)
(1 )
( ) ( )
( ...)
( ...)
( ...)
( ...)
( ...)
o o o
o o s
o m s s o m
o o o
o o o o
s s s s
o
o o o o
m m m m
d F pF p F
dx
p
B B
B B B B B B
d F pF p F
dx
F pF p F B
p
F pF p F B
F pF p F B
F pF p F B
(
+ + +
(
(
(
(
+ + + + (
+ + +
+ + +
+
+ + +
| |
+ + +
|
|
+ + + +
\ .
0 1 2
0 1 2
1 2 3
0 1 2
2
2
2
2
( ...)
( ...)
( ...)
( ...)
s s s s
s s s s
o o o o
m m m m
F pF p F B
F pF p F B
F pF p F B
F pF p F B
(
(
(
(
| |
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
+ + +
(
|
(
|
+ + + +
(
|
(
|
| |
+ + +
|
|
+
| |
+ + + +
\ . \ .
0 =
(
(
(
(B.11)
Substituting the eqns.(B.7)-(B.9) into an eqn.(B.6) we get
0 1 2
2 2
2
2
( ...)
(1 )
( ) ( )
m m m
m m s
o m s s o m
d F pF p F
dx
p
B B
B B B B B B
(
+ + +
(
(
(
(
+ + + + (
Frontiers in Sensors (FS) Volume 2, 2014 www.seipub.org/fs
41
0 1 2
0 1 2
0 1 2
1 2 3
0 1 2
0 1 2
0 1 2
1 2 3
2 2
2
2
2
2
2
2
2
2
2
( ...)
( ...)
( ...)
( ...)
( ...)
( ...)
( ...)
( ...)
(
m m m
m m m m
s s s s
m
o o o o
m m m m
s s s s
s s s s
o o o o
m
d F pF p F
dx
F pF p F B
p
F pF p F B
F pF p F B
F pF p F B
F pF p F B
F pF p F B
F pF p F B
F
+ + +
+ + +
+
+ + +
| |
+ + +
|
|
+ + + +
\ .
+ + +
+ + + +
+ + +
+
+
0 1 2
2
0
...)
m m m
pF p F B
(
(
(
(
| |
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
= (
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
(
|
| |
(
|
|
(
|
|
+ + +
(
\ . \ .
(B.12)
Comparing the coefficients of like powers of p in (B.10)
we get
0
2
0 2
2
( )
: 0
( ) ( )
s
o m s
s
o m s s o m
d F
B B B
p
B B B B B B dx
( +
=
(
+ + + +
(B.13)
The initial approximations are as follows
0 1
(1) 1; ' (0) 0
s s
F F = = (B.14)
(1) 0; ' (0) 0 , 1,2,3,...
i i
s s
F F i = = = (B.15)
Solving the eqn. (B.13) and using the boundary
conditions eqns.(B.14)-(B.15), we obtain the following
result:
0
2
2 1
( ) ( 1) 1
2
s s
k
F x F x = = + (B.16)
Where
2
1
k is defined in the text eqn. (21).
After putting the eqn. (B.16) into an eqn. (B.7), we
obtain the solution in the text eqn.(18).
Comparing the coefficients of like powers of p intoan
eqn.(B.11) we get
0
2
0
2
2
:
0
( ) ( )
o
o s
o
o m s s o m
d F
p
dx
B B
B B B B B B
(
=
(
+ + + +
(B.17)
The initial approximations are as follows
0 0
(1) 1; ' (0) 0
o o
F F = = (B.18)
(1) 0; ' (0) 0, 1,2,3,...
i i
o o
F F i = = = (B.19)
Solving the eqn. (B.17) and using the boundary
conditions eqns. (B.18)-(B.19), we obtain the following
result:
0
2
2 2
( ) ( 1) 1
2
o o
k
F x F x = = + (B.20)
Where
2
2
k is defined in the text eqn.(22).
After putting the eqn.(B.20) into an eqn.(B.8), we
obtain the solution in the text eqn.(19).
Comparing the coefficients of like powers of p into an
eqn.(B.12) we get
0
2
0
2
2
:
0
( ) ( )
m
m s
m
o m s s o m
d F
p
dx
B B
B B B B B B
(
=
(
+ + + +
(B.21)
The initial approximations are as follows:
0 0
(1) 1; ' (0) 1
m m
F F = =
(B.22)
(1) 0; ' (0) 0
i i
m m
F F = = (B.23)
Solving the eqn.(B.21) and using the boundary
conditions eqns.(B.22)-(B.23), we obtain the following
result:
0
2
2 3
( ) ( ) 1
2
m m
k
F x F x x = = +
(B.24)
Where
2
3
k is defined in the text eqn. (23).
After putting the eqn.(B.24) into an eqn.(B.9), we
obtain the solution in the text eqn.(20).
Appendi x C: Sc i l ab/Mat l abpr ogr am f or t he
Numer i c al Sol ut i on of t he Syst ems of
Non-l i near Di f f er ent i al Eqns. (12)-(16)
function pdex4
m = 0;
x = linspace(0,1);
t = linspace(0,100000);
sol = pdepe(m,@pdex4pde,@pdex4ic,@pdex4bc,x,t);
u1 = sol(:,:,1);
u2 = sol(:,:,2);
u3 = sol(:,:,3);
%plot(x,u1(end,:))
%xlabel('Distance x')
%ylabel('u1(x,2)')
%figure
www.seipub.org/fs Frontiers in Sensors (FS) Volume 2, 2014
42
%plot(x,u2(end,:))
%xlabel('Distance z')
%ylabel('u2(x,2)')
%figure
%plot(x,u3(end,:))
%xlabel('Distance x')
%ylabel('u3(x,2)')
% --------------------------------------------------------------
function [c,f,s]=pdex4pde(x,t,u,DuDx)
c = [1;1; 1];
f = [1; 1; 1] .* DuDx;
Bs=0.5; B0=0.05; Br=0.1;
a1=sqrt(0.001); a2=sqrt(0.001); a3=sqrt(0.001);
F1 =-a1^2/(1+(1/(u(2)*B0+u(3)*Br))+(1/(u(1)*Bs)));
F2 =-
(a2^2*(u(2)*B0)/(u(2)*B0+u(3)*Br))*(1/(1+(1/(u(2)*B0+u(
3)*Br))+(1/(u(1)*Bs))));
F3 =-
(a3^2*(u(3)*Br)/(u(2)*B0+u(3)*Br))*(1/(1+(1/(u(2)*B0+u(
3)*Br))+(1/(u(1)*Bs))));
s = [F1; F2; F3];
% --------------------------------------------------------------
function u0 = pdex4ic(x)
u0 = [1; 1;1];
% --------------------------------------------------------------
function [pl,ql,pr,qr] = pdex4bc(xl,ul,xr,ur,t)
pl = [0; 0; ul(3)-1];
ql = [1; 1; 0];
pr = [ur(1)-1; ur(2)-1; ur(3)-1];
qr = [0; 0; 0];
Gr eek Symbol s
2
s
Thiele modulus for the substrate (Normalized)
2
o
Thiele modulus for the oxygen (Normalized)
2
m
Thiele modulus for the mediator (Normalized)
Subsc r i pt s
o Oxygen
s Substrate
m Mediator
OX
Oxidized species
red Reduced species
t Total
Bulk solution
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Ananthaswamy V., and Rajendran L.,Analytical solutions
of some two-point non linear elliptic-boundary value
problems. Applied Mathematics. 3 (2012): 1044-1058.
Ananthaswamy V., and Rajendran L., Analytical solution of
Non-isothermal diffusion-reaction processes and
effectiveness factors. ISRN Physical chemistry, Hindawi
publishing corporation. 2012 (2012): 1-14.
Ananthaswamy V., Ganesan SP., and Rajendran.,
Approximate analytical solution of non-linear boundary
value problem of steady state flow of a liquid film:
Homotopy perturbation method. International Journal
of Applied Science and Engineering Research. 2 (5)
Appendi x D: Nomenc l at ur e
Symbol Meaning
| |
t
E
Total active enzyme concentration in the matrix
(mmol/L)
| |
OX
E
Enzyme concentration of the oxidized mediator
(mmol/L)
| | ES
Enzyme concentration of the substrate (mmol/L)
| |
red
E
Reduced enzyme concentration (mmol/L)
| |
2
O
Concentration of oxygen at any position in the enzyme
layer (mmol/L)
| |
2 b
O
Oxygen concentration in the bulk electrolyte (mmol/L)
| |
2
O
Oxygen concentration in the bulk solution (mmol/L)
| | S
Concentration of substrate at any position in the
enzyme layer (mmol/L)
| |
b
S
Substrate concentration in the bulk electrolyte
(mmol/L)
| |
OX
Med
Concentration of oxidised mediator at any position in
the enzyme layer(mmol/L)
| |
OX b
Med
Oxidised mediator concentration in the bulk
electrolyte (mmol/L)
| |
OX
Med
Oxidized mediator concentration in the bulk solution
(mmol/L)
o
D Diffusion coefficient of oxygen (cm2 s-1 )
s
D
Diffusion coefficient of substrate (cm2 s-1 )
m
D
Diffusion coefficient of mediator (cm2 s-1)
d Enzyme layer thickness (cm)
y
Distance from the electrode (cm)
1 4 5
, , k k k
Rate constants ( L mol-1 s-1 )
2 5
, k k
Rate constants ( s-1 )
o
K
Partitioning coefficient for oxygen (none)
s
K
Partitioning coefficient for substrate (none)
m
K
Partitioning coefficient for mediator (none)
o
B
Normalized surface concentration of oxygen
s
B
Normalized surface concentration of the substrate
m
B
Normalized surface concentration of mediator
s
F
Normalized surface concentration
o
F
Normalized oxygen concentration
m
F
Normalized mediator concentration
Frontiers in Sensors (FS) Volume 2, 2014 www.seipub.org/fs
43
(2013): 569-577.
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of oxygen, Biosens Bioelectron,10 (1995): 393-403.
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Oxidase Enzyme Electrode in the Presence of Two
Oxidants Anal Chem, 66 (1994):2763-2770.
Mousa M.M., Ragab S.F., and Nturforsch Z., Application of
the Homotopyperturbation method to linear and
nonlinear Schrdinger equations .Zeitschrift fr
Naturforschung. 63 (2008): 140-144.
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chloroosmium (+/2+) complexed poly(1-vinylimidazole)
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Ozis T., and Yildirim A., Relation of a Non-Linear
Oscillator with Discontinuities.Int. J. Nonlinear. Sci.
Numer. Simulat.8(2) (2007): 243-248.
Pallachi G., and, Turner A.P.F., Amperometric
tetrathiafulvalene-mediated lactate electrode using
lactate oxidase absorbed on carbon foil,Anal Chim Acta,
234 (1990): 459-463.
Scheller F., Schulbert F., Biosensors, Techniques and
Instrumentation in Analytical Chemistry, Elesvier,
Amsterdam, 1992.
Schulmeister T., Mathematical modeling of the dynamic
behaviour of amperometric enzyme electrodes,
Selective Electrode Rev., 12 (1990): 203-260.
Schulmeister T., and Pfeiffer D., Mathematical modelling of
amperometric enzyme electrodes with perforated
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E., Halliwell C.M, Seng Toh C., Cass A.E.G, and P.N.
Bartlett, Immobilisation of enzymes on poly (aniline)
poly (anion) composite films. Preparation of bioanodes
for biofuel cell applications, Bioelectrochemistry, 55
(2002): 13-15.
Trevan M.D., Immobilised enzymes, 2nd edn. Wiley, New
York, 1981.
Dr. V. Ananthaswamy received his
M.Sc. Mathematics degree from
The Madura College
(Autonomous), Madurai-625011,
Tamil Nadu, India during the year
2000. He has received his M.Phil
degree in Mathematics from
Madurai Kamaraj University,
Madurai, Tamil Nadu, India
during the year 2002. He has
received his Ph.D., degree (Under the guidance of Dr. L.
www.seipub.org/fs Frontiers in Sensors (FS) Volume 2, 2014
44
Rajendran, Assistant Professor, Department of Mathematics,
The Madura College, Tamil Nadu, India) from Madurai
Kamaraj University, Madurai, Tamil Nadu, India, during the
year October 2013. He has 14 years of teaching experiences
for Engineering Colleges, Arts & Science Colleges and
Deemed University. He has 3 years of research experiences.
At present he is working as Assistant Professor in
Mathematics, The Madura College (Autonomous), Madurai-
625 011, Tamil Nadu, India from 2008 onwards. He has
published more than 23 research articles in peer-reviewed
National and International Journals and communicated 7
research articles in National and International Journals.
Presently he has Reviewer/Editorial Board
Member/Advisory Board Member in 43 reputed National
and International Journals. Currently he has doing one
ongoing minor research project sanctioned at UGC. His
present research interest includes: Mathematical modeling
based on differential equations and asymptotic
approximations, Analysis of system of non-linear reaction
diffusion equations in physical, chemical and biological
sciences, Numerical Analysis, Mathematical Biology,
Mathematical and Computational Modeling, Mathematical
Modeling for Ecological systems. Also, he has participated
and presented research papers in National and International
Conferences.
Mrs. M. Subha received her M.Sc.,degree
(2012) and M.Phil., degree (2013) in
Mathematics from The Madura College
and E.M.G. Yadhava Womens College,
Madurai, Tamilnadu, India. At present,
She is working as Assistant Professor in
the Department of Mathematics, Madurai
Sivakasi Nadars Pioneer Meenakshi Womens College,
Sivagangai District, Tamil Nadu, India. Also, she is doing
her Ph.D entitled Asymptotic Methods for Solving Initial
and Boundary Value Problems at Madurai Kamaraj
University, Madurai under the guidance of Dr. L. Rajendran,
Assistant Professor, Department of Mathematics, The
Madura College, Madurai. Her present research interest
include: Mathematical modelling, Analytical solution of
system of nonlinear reaction diffusion processes in
biosensor, Homotopy analysis method, Homotopy
perturbation and numerical methods. She has published 4
papers in International Journals and communicated one
research paper in National Journal. Also, She has
participated and presented research papers in International
and National Conferences.
Ms. C. Chowmiya received her M.Sc.,
degree in Mathematics from Sri
Meenakshi Government Arts and Science
College for Women (Autonomous),
Madurai, Tamil Nadu, India during the
year 2013. She has completed her M.Phil.,
(Mathematics) from The Madura College
(Autonomous), Madurai, Tamil Nadu,
India during the year2014. At present, She is working as
Assistant Professor in the Department of Mathematics, Syed
Ammal Engineering College, Ramanathapuram District,
Tamilnadu, India. She has presented a paper at National
Conference. She has published one National and
International Journals. Her research area includes;
Mathematical Modeling for solving Biosensor problems,
Solving Non-linear differential equations by Numerical and
Asymptotic Methods.