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2014

MSc Biochemistry
1
st
Paper
SLQS
1. 3 types of chemical bonds stabilizing protein structure. (5)
2. Study of structure and function of protein in (7)
a. Muscle contraction
b. Enzyme regulated by change in conformation
3. Electrophoresis and chromatography in diagnosis/separation of Hb varients and
hemoglobinopathies. (8)
MEQS
BRCA gene expression in breast cancer
4. Specimen and genetic material to be taken for diagnosis. (3)
5. Molecular technique for study of gene expression (8)
6. Immunoassay technique in gene expression (5)
7. Primer design for gene amplification (tools and techniques)
Short answers (6*5=30)
8. Principle and clinical use of UV-VIS
9. Application of MS in clinical medicine
10. Detection of end group of protein
11. Derivation of Henderson-hasselbasch equation and use in clinical chemistry.
12. Flame photometry and ISE principle and use
13. Predictive value in diagnostic efficiency by 2*2 table

2014
MSc Biochemistry
2
nd
Paper
MEQ
1. Steps of glycolysis (5)
2. Regulation of glycolysis (3)
3. Role of krebs cycle in intermediary metabolism (7)
4. Glucose regulation in nonfed stage (5)
5. Alcohol consumption knee pain in morning
a. Preliminary diagnosis
b. Mechanism of disease (3)
c. Two drugs used and their mechanism (4)
d. Denovo synthesis of purine (11)
SAQ (6*5=30)
6. Write about the role of transamination in liver.
7. Role of cytochrome 450 in xenobiotics
8. B-oxidation of palmitic acid and bioenergetics.
9. Role of Vit in antioxidant
10. Role of HDL in Human body
11. Derive Mechalis-menten equation and its uses.

MCQs
1. Effector molecule gluconeogenesis- AcetylcoA
2. A: 17 ketosteroid is used for gonadal function
R: Synthesized by gonad only
3. A: Catalase and SOD are chain breaking antioxidant
R: Both are affected by EDTA
4. Glucogenic AA T/F
5. Fatty acidsynthesis vit. Niacin
6. Pyruvic acid accumulation: - Thiamine
7. Marker of protein synthesis used in Nutrient supplementation
8. Which of the following is the product of pancreatic fluid
9. Which of the following is product of pyrimidine degradation?
a. B-alanine, b. hydro c. Uric acid
10. Part of insulin most conserved
11. Component between Injector and column
12. Purification by adsorption method: enzyme, hormone, nucleotide coenzyme
13. Neural tube defect
14. Which of following is secondary data?

2014
MSc Biochemistry
3
rd
Paper
SLQS
1. Watson and Crick DNA structure
2. Semiconservative mode of replication : experiment
3. Study of structure and function of DNA in disease
4. Replication fidelity explain
MEQS
15 days starvation case
5. Ketogenesis (2)
6. What are ketone bodies? Ketone body metabolism (5)
7. Biochemical basis of gluconeogenesis and lipolysis (7)
8. Acid/base status of patient (2)
9. Urine NPN role (3)
Short answer
10. Biochemical changes in menstrual cycle
11. Antigen processing and presentation
12. Techniques for monoclonal Ab production and its use
13. Role of incretins
14. Gene therapy