43

The 5
th
Annual Northeast Pharmacy Research Conference of 2013 16 17 2556
Pharmacy Profession: Moving Forward to ASEAN Harmonization
______________________________________________________________________________________________

2
P1-PS-3

, *, ,
, ,

:

loop diuretics
(40 500 ) (10
/ )

:
1
2


:
0.7 %



(7.80 0.06) 1
2
90
35 1

8.0 1
HPLC
35 (89.97 95.50
, P=0.27) 2
200 MPN/mL :




: ,

____________________________________________________________


44150
* : Tel: 080-4137996, E-mail: topaz_killer@hotmail.com
P1-PS-4
Quality Control For the Mixture of Phet Sang-Khaat
Capsule
Thitiphong Phanharach*, Kritsada Artitruangroj, Somchai Sopajan,
Wanida Caichompoo , Kreephon Manwivattanagun

Introduction: The mixture of Phet Sang-Khaat capsule is
recommended to treat hemorrhoid in Thai National List of
Essential Herbal Medicines (2011). This formula consists
of 3 medicinal plants including Cissus quadrangularis
(Linn.) (Vitaceae), Clerodendrum serratum (L.) Moon. var.
wallichii C.B.Clarke (Lamiaceae) and Rheum officinale
Baillon. (Polygonaceae). The previous review literatures on
the mixture of Phet Sang-Khaat capsule did not contain
information about quality control or formulation. However,
we found several phytochemical and pharmacological
studies on single ingredients of this formula. The aim of
the study to determine the contents of some active
constituent in each ingredient of formulation such as
quercetin in C. quadrangularis and rhein in R. officinale and
the mixture of Phet Sang-Khaat capsule. Methods: The
dried powder of the samples were extracted with 50%
ethanol. HPLC was used to determine each of medicinal
plants preparation content of major compounds from these
3 medicinal plants from 5 different regions. Results: The
results showed that the amount of quercetin in C.
quadrangularis from Khon Kaen, Nakornratchasima, Maha
Sarakham, Phetchabun and Roied provinces were 1.8,
1,69, 3.51, 3.15 and 3.30 (%w/w, dried powder),
respectively. The amount of rhein in rhubarb from
Bangkok, Khon Kaen, Maha Sarakham, Nakornratchasima,
and Roied provinces were 1.02, 1.09, 1.01, 0.54 and 1.56
(%w/w, dried powder), respectively. Thus, we were used
the aerial part of C. quadrangularis from Maha Sarakham
and the rhizomes of rhubarb from Roied province as the
ingredients to formulate the mixture of Phet Sang-Khaat
capsule in this study. The amount of querceitn and rhein in
the mixture of Phet Sang-Khaat capsule were obtained
10.96 and 0.70 mg/capsule, respectively. Conclusion: The
results from this study could be used as a guideline for the
quality of some Thai herbal formulation.

Keywords: quality control, the mixture of Phet Sang-
Khaat capsule, quercetin, rhein
____________________________________________________________

Faculty of Pharmacy, Mahasarakham University, Mahasarakham Province,
44150, Thailand
*Corresponding author: Tel: 089-9414725 E-mail: woonupk@hotmail.com

The 5
th
Annual Northeast Pharmacy Research Conference of 2013 Pharmacy Profession:
Moving Forward to ASEAN Harmonization Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand
February 16 17, 2013
February 16 17, 2013 The 5
th
Annual Northeast Pharmacy Research Conference of 2013
Pharmacy Profession: Moving Forward to ASEAN Harmonization
Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand
_______________________________________________________________________________________________

43
O1-PS-2
Development of Soy Isoflavone Body Scrub
Formulations
Lalita Leksantikul, Ekapol Limpongsa
1,2
, Napaphak Jaipakdee
1,2
*

Introduction: Isoflavones are more attention in
cosmeceuticals regarding the skin care benefit effects.
Scientific evidences demonstrated the potential use of
isoflavones in the prevention and treatment of numerous skin
disorders such as skin aging, cellulite, acne and sensitive
skin. Recently, scrub products have gained popularity and
gradually grown in the mass market. Moreover, scrub
products provide cleansing and skin rejuvenation benefits.
Therefore, the objective of this study was to develop the soy
isoflavone body scrub formulations contained soybean
extract and powder of soybean seed. Materials and Method:
Isoflavones were extracted from soybean powder by using
ethanol-water system. After isoflavone extraction, the soy
powders were dried and sieved. The oil in water emulsion
base with optimum viscosity and good skin hydration effects
was formulated and prepared by using beaker method. The
soy isoflavone body scrubs containing soy extract and soy
powder were prepared. The physical appearance, pH,
viscosity, and physical stability of soy isoflavone body scrubs
were determined. Results: The pH and viscosity of
emulsion base were 7.77 0.03 and 2.19 0.20 x 10
6
cps,
respectively. Incorporation of soy isoflavone and powder of
soybean seed significantly decreased the pH value, but had
insignificantly effect on the viscosity of the emulsion base.
The developed soy isoflavone body scrubs had a good
physical stability at accelerated, heating (45C, 24h) and
cooling (2C, 24h) for 6 cycles, and at ambient conditions (3
month). No phase separation or bleeding occurred. The pH
and viscosity did not change significantly. The preliminary
sensory assessment in 14 healthy volunteers revealed that
sizes and amount of soy powder in soy isoflavone scrubs
affected their preferences. Conclusion: The developed soy
isoflavone body scrub formulations had a good appearance
and physical stability. Further studies regarding the chemical
stability of soy isoflavones in the scrub formulations, as well
as the efficacy evaluations such as skin hydration shall be
performed.
Keywords : Body Scrubs, Isoflavones, Soy powders
____________________________________________________________
1
Division of Pharmaceutical Technology, Faculty of Pharmaceutical
Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
2
Center for Research and Development of Herbal Health Products,
Khon Kaen University, Khon Kaen 40002, Thailand
*Corresponding author: Tel (66 43) 362092, Fax (66 43) 362092,
Email : jsaowanee@kku.ac.th
O1-PS-3
Effect of Pueraria mirifica and miroestrol on estrogen deficiency
in ovariectomized mice
Atcharapon Luitan, Orawan Monthakantirat, Yaowared Chulikhit *

Introduction: Estrogens are important hormonal signals and
playing a critical role in reproductive system as well as non-
reproductive system including central nervous system (CNS).
Decreasing of estrogen in menopause women may associate
with depression and cognitive impairment. Ovariectomized
(OVX) mice, the estrogen deficiency model exhibited the
depressive and cognitive impairment behaviors. Estrogen plays
an important role in neurogenesis and synaptic plasticity of the
neuron. Brain-derived neurotrophic factor (BDNF) is also
promotes neurogenesis. cAMP response-element-binding
protein (CREB) is an important regulator of the transcription of
the BDNF gene and reduction of CREB resulting in decrease
level of BDNF. Objective: This study is interested in effects of
hormone replacement therapy (HRT), 17-estradiol (E
2
) and
phytoestrogen, Pueraria mirifica crude extract and miroestrol on
gene expression including BDNF, CREB in OVX mice
correlated with depression and cognitive impairment. Materials
and methods: Three week old female ICR mice were
divided into sham and OVX groups. For OVX groups, the
animals were divided into 6 groups (n = 3-6). Group 1 received
only corn oil. Group 2 was administered with E
2
(10 g/kg, i.p.).
Group 3-4 were administered with Pueraria mirifica crude
extract (10 and 25 mg/kg, p.o , respectively) and group 5-6
were administered with miroestrol (0.1 and 1 mg/kg, i.p. ,
respectively). Level of estrogen serum was determined by using
Electrochemiluminescence immunoassay. Gene expression of
BDNF and CREB were determined by using Reverse
transcription polymerase chain reaction (RT-PCR). Results:
OVX mice showed significantly decreasing in level of estrogen
serum and the expression of CREB and BDNF in hippocampus
and frontal cortex. After mice received Pueraria mirifica crude
extract (10 and 25 mg/kg, p.o) and miroestrol (0.1 and 1 mg/kg,
i.p.) for ten weeks, the level of estrogen serum was increased
as similar as E
2
. The expression of CREB and BDNF in the
brain of OVX mice was increased after received Pueraria
mirifica crude extract and miroestrol. Conclusion: The finding
support that Pueraria mirifica crude extract and miroestrol are
good phytoestrogen to increase level of estrogen and gene
related to neurogenesis which might be improve depression
and cognitive impairment.

Keywords: Ovariectomized mice, 17-estradiol, Pueraria
mirifica, miroestrol, CREB and BDNF
____________________________________________________________
Faculty of Pharmaceutical Sciences, Khon Kaen University,
Muang District, Khon kaen 40002
*Corresponding author: Tel: 043-202378, 043-362094 Fax: 043-20 2379,
E-mail: yaosum@kku.ac.th