How Tech Could Spell The End of Animals in Drugs Testing - Science - The Observer

9/10/14 How tech could spell the end of animals in drugs testing | Science | The Observer
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'Would y ou be happy standing up in a court of law to explain why y ou hadn't tested this drug on animals?'
say s Dr Kathry n Chapman. 'There is a powerful cultural mindset about how y ou do drug dev elopment.'
Photograph: Alamy
Think of a world where more new medicines are available more quickly, more safely and
with few, if any, animals used in research. Think of a world where you know for sure that
the drug you are prescribed is safe for you. This is what researchers at some of the
world's leading universities are trying to bring about.
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How tech could spell the end of animals
in drugs testing
We are in a pharmaceutical ice age where fewer drugs are being
developed. But new computer modelling and bioengineering
techniques could revitalise the industry and make animal testing
obsolete
Mark Piesing
The Observ er, Saturday 23 August 201 4 20.00 BST
Academics from disciplines as far apart as computer science and biology are growing
mini-human hearts in a lab or creating a model of the human biological system on a
common computer chip. Their aim is to bring to an end to what has been called the
"pharmaceutical ice age", an era of ever more research but fewer medicines reaching the
shelves of Boots.
According to the Association of the British Pharmaceutical Industry, it can take up to 12
years and 1.15bn before a new medicine reaches the high street. For that one medicine,
25,000 chemicals will have been tested, 25 potential drugs will have had human clinical
trials, perhaps five will have been passed by the regulators and only one will have been
marketed by a drugs company. Even after that, a good number of drugs have to be
withdrawn owing to previously undetected side-effects.
For some, the fault for this freeze is due to the pharmaceutical industry's obsession with
mergers and acquisitions or a culture that inhibits innovation. For others, it is a sign that
perhaps there just aren't many chemical compounds that can interact with the body and
have a positive effect; or even that the tools scientists use during discovery and early-
stage testing mean the wrong drugs go to human trials.
While animal testing is a regulatory requirement for any new drugs, it remains a fairly
unpredictable guide to how a drug will react in humans. Testing drugs on cells in a petri
dish allows much higher dosages than with an animal, but is an unreliable guide as to
how they might interact with the human body. Virtual computer models of the likely
impact of a drug have also, up to now, largely been based on the reactions of a single cell.
Now scientists are trying to use new technology to make drug testing faster, cheaper
and more accurate by modelling more closely how a chemical interacts with the human
body. They hope to do this to the point where a drug could be declared safe for a certain
section of the population or even personally safe for you.
"Over the past 1520 years, there has been a dramatic increase in results from
biomedical research," says Richard Seabrook, head of business development at the
Wellcome Trust. "However, this hasn't led to an equally dramatic increase in new
medicines approved.It has been pretty static in Europe and the US. This might be
because it is a relatively rare event for a small chemical to interact with the human body
and have a positive effect."
Dr Kathryn Chapman, head of innovation and translation at NC3Rs, the National Centre
for the Replacement, Refinement and Reduction of Animals in Research, says: "There
has been a pharmaceutical ice age over the last 2025 years. So people have been trying
to find new ways of doing things, as the old ways don't work, and the money is there for
this. The issue with all these technologies is how to add on the complexity."
"The problem has been that the discovery of new drugs hasn't been given as much like-
by-like value by analysts as the development of drugs that can shift the share price,"
says Professor S John Lyon of Warwick Business School. "The development of new drugs
also has to follow strict regulations laid down by bodies like the FDA."
The tag line for the Wyss Institute at Harvard is "biologically inspired engineering".
Professor Donald Ingber, the institute's founding director, is fulfilling this aim by being
one of the pioneers of the concept of an "organ on a chip", which involves placing the
human cells and fluids necessary to mimic the complexity of human physiology on a
single, tiny piece of see-through, flexible silicone rubber the size of a USB stick. In his
new startup, Emulate, these chips will be mass-produced for the first time.
The chips have fluid flowing through them, so you can connect them to each other,
creating a "human on a chip" where lungs, livers, intestines, skin, kidneys and eyes can
be integrated to simulate how a whole body would interact with a new drug.
"You could write a 2,000-word article just on this pharmaceutical ice age alone," laughs
TED speaker Dr Geraldine A Hamilton who works with Ingber to develop the technology
and commercialise it, and who move to become president and CSO of Emulate in the
coming months.
"The structures you find yourself working in can stifle innovation. Every time companies
merge, productivity goes down. Then there has been a problem with the tools they use
during testing. Often, cells are put in a simple petri dish, but they live in a complex
dynamic organic environment with each other. Animals can fail to predict in humans how
drugs will work. Different animal species may give you different answers as to whether a
drug is toxic for humans. Which one do you trust? Many good drugs are lost at that
point.
"So we recreate the conditions the cells find themselves in by using micro-engineering to
provide them with all the cellular integration, fluids and even mechanical forces that
they are used to, such as breathing in and out. We can also connect them in our human
bioemulation platform to model and better understand diseases, and to study how
humans respond to drugs."
Until now, the complexity of chips meant they had to be made by hand, limiting their
numbers. It also introduced variability, whereas they need to be reproducible and
robust. So, in partnership with Sony, the team has worked on ways of scaling up the
manufacture. This is the key to translating this technology to industry, Hamilton
believes.
Illustration by Dale
Edwin Murray.
Meanwhile, in the labs of Abertay University, Dundee, Professor Nikolai Zhelev has
mass produced thousands of miniature hearts 1mm across that can beat 30 times a
minute. "This is the first time it's been possible to induce diseases in them, so it's an
exciting area of research," says Zhelev. "After they start beating, we use chemicals to
make the cells become hypertrophic enlarged and then we test different compounds
and drugs on them to see if we can prevent them from becoming hypertrophic or even
everse the damage.'
Biosensors or markers enable his team to track individual molecules to see which cause
the hearts to become enlarged so they can target new drugs just at these molecules. The
implications of this are important. For example, they are developing a cancer drug that
is already in human trials and initial tests suggest it could work on full-size hearts as
well.
"Mini hearts are much better than using animals to test drugs they are closer to the
real thing and they should speed up the time it takes to move towards clinical trials,"
says Zhelev.
"Our mini hearts are a real biological system providing relevant answers to drug
screening efforts. Virtual drug testing faces enormous challenges in such complex
systems. However, this is an area which is advancing rapidly with the development of
mathematical and computational modelling approaches."
Far away from the petri dishes and lab coats, but just around the corner from Oxford
University's animal testing laboratory and the protesters outside it, researchers in the
Department of Computer Science believe that they have made a breakthrough in the use
of virtual computer models to simulate heart cells and eventually skin, brain cells, and
whole organs, for the faster and more accurate testing of the toxicity of drugs at an early
stage. Their research to develop the Virtual Assay software has been funded by the
Engineering and Physical Science Council.
Rather than moving their research into the private sector, they have decided to stay
within the university and will release their software free for academics in September and
in due course it may be licensed as an app-style product to the pharmaceutical industry.
"It is exciting to feel that you are exploring a genuine frontier," says PhD student Oliver
Britton, one of five finalists in the Transforming Society category of the 2014 UK ICT
Pioneers competition and on whose research this project is based.
Virtual computer models comprise a large collection of equations that describe how
components of a cell interact with one another and with physical quantities such as the
voltage of the cell's membrane or concentrations of chemicals such as calcium and
potassium.
"We solve these equations using computers and doing this allows us to simulate how a
heart cell might behave in different conditions," says Britton.
"A lot of scepticism about the accuracy and usefulness of virtual models comes from a
misunderstanding of how they should be used," says Blanca Rodriguez, Wellcome Trust
senior research fellow in basic biomedical sciences. "If you ask a model to do something
and it can't then you will be disappointed." In the past, she explains, a model might use
one cell so when you did the computational experiment you got an output that
corresponded to just one cell. "If you did an actual experiment, you would never trust
the outcome of just one cell because there is a high degree of variability."
"So we came up with this idea of using a population of varied models," says Britton. "This
was the key step, as we can achieve more accurate results by first generating lots of
different models of a human heart cell, then simulating how they would behave in control
conditions and then discarding those that don't fit the range of experimental data we
have available."
"The challenge is to understand the limitations of the model such as in terms of what it
can predict and then start to refine it," says Rodriguez. "The success of it will depend on
how easy it is to use."
Seabrook warns that it is "a long way off" before any of these technologies are accepted
instead of animals. "There needs to a lot of very good evidence that the result of using
these technologies is predictive of what is happening in the human body."
The problem, he says, is that predictions about the impact of a new drug are built on
existing evidence. "So you don't know what you don't know, and you don't know how a
chemical will behave in a body until you do it."
"If you think about it, a pill is swallowed to get into the intestine, absorbed through the
gut and then has to get to where the disease is, whether in the brain, eye or lungs. So
during this process chemicals are exposed to proteins, enzymes and cells so it is hard for
any technology to predict reliably how it will interact with human body."
He accepts these technologies mean we should get there quicker and so the more
technology is available the quicker the process will be, and the easier it will be, to put
your effort into promising candidates for new medicines. "If you are going to fail, fail
early," he says.
The drive to melt the ice age has also encouraged the development of new approaches
such as those that use DNA and human proteins. Kathryn Chapman agrees that the
technology is as yet unproven and years of data will be needed before you can use it to
really start to reduce the number of experiments on animals.
"There is a powerful cultural mindset about how you do drug development. It is the
courtroom argument. Would you be happy standing up in a court of law to explain why
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you hadn't tested this drug on animals?
"Still, if the process changes and allows the technology to be taken up then the outcome
will hopefully be better drugs and faster."
In June, the US Food and Drug Administration (FDA) proposed changes to how
molecular entities are assessed that could open the door to new tools such as
VirtualAssay. Ultimately, new techniques may allow the personalisation of medicine, so
"you may even be able to use your own stem cells to test side-effects on you. That will
be a massive shift," says Seabrook.
For Bina Rawal, research director at the Association of the British Pharmaceutical
Industry, this is all part of a "tremendous acceleration" in new approaches to testing.
This is indicative of "a cultural shift within pharma too," she says. "The model has been
adapted to be all about collaborations, working together and open innovation. This
industry is traditionally very competitive, but now in the pre-commercial stage there is
more sharing to stop everyone going up a blind alley."
Perhaps the ice age is ending.
* When this article was first published it included an incorrect figure for the cost of
developing a new medicine, the correct figure is 1.15bn, this was amended on 27
August 2014.
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