title creator

The effect of Binahong

(Anredera cordifolia(Ten)
Steenis) Leaves Extract to
the Level of Male Rat
Serum TNF- Which is
Induced by Formaldehyde
Chandra
Wahyu
Kurniawan
description publisher
Formaldehyde is one of the hazardous food preservative, its because of the residual
metabolite from this compound is carsinogenic if its also generate free radicals very reactive
to cause cellular damage of macromolecules and inflammation, then the cells mights
eventually die, so the organ dysfunction will be the worst result of it. Binahong leaves
(Anredera cordifolia(Ten) Steenis) contains flafonoids as their main secondary active
metabolite which is very potential to play a big role as the antioxidant. Therefore, the author
wants to prove the effect of binahong leaves extract as an antioxidant to the level of serum
TNF- in maximum formaldehyde induced male rat.
Laboratory experimental non-randomized post-test only control group is chosen as the
design of this research. This research is held in 2 step of action. The first step is the
optimization of formaldehide dose, and the second step is the optimization of Binahong
leaves extract dose. In the first step of research, rats are treated with 1ml formaldehyde for
each rat per day in 7 days, in the range of dosage 0 ppm(as the control), 25 ppm, 50 ppm, and
75 ppm. In the second step of the research, the rats are treated with the effective dose based
from the result of the effective dose in the first step, and the rats are also treated with the
Bimahomg leaves extract in the range of dose (25%, 50%, 100%) within the result is read by
ELISA reader in the wave lenght of 450nm. The data is statistically analized with oneway
ANOVA test then further its analyzed with Post Hoc LSD test. The result are catagorized as
significan p if p < 0,05.
Formaldehyde administration in the dosage 25 ppm, 50 ppm and 75 ppm. Significantly shew
the increase of serum TNF- (p 0,05) and the result of serum TNF- respectively are
(2197,57 IU/ml; 2458,65 IU/ml; 3065,88 IU/ml. After the administration of Binahong
extract, the result of serum TNF- respectively are 25% (2316,00 IU/ml), 50% (2464,00
IU/ml) & (1825,34 IU/ml). The result is that the Binahong leaves extract significantly reduce
the serum TNF- of the rat which is incuded by 75 ppm formaldehyde compared to the
positive control group (3171,34 IU/ml). The second result is that the administration of 25%
extract of Binahong leaves is more effective to reduce serum TNF- compared to the 50%
extract of Binahong leaves. The third result is that the maximum reduction of serum TNF-
were found at thr serum of rat which are treated with 100% extract of Binahong leaves.
Formaldehyde administration is the range of (25 ppm, 50 ppm and 75 ppm) might increase
the rat serum TNF- , with the 75 ppm as the optimum dose. Administration of Binahong
leaves extract in the dosage of 25%, 50% & 100% may decrease the rat serum level of TNF-
, with the 100% extract dose as the most effective dose.
contributor date type permalink right journal
Jurnal
Supervisor: Ariani
Ratri Dewi, Co-
Supervisor: Yoyon
Arif Martino