Biochemistry (CVS ) (Lecture 1-4)

1. Regarding apoproteins;
A. Apo C11 activates lipoprotein lipase
B. Apo D is part of VLDL
C. Apo B48 is part of chylomicrons
D. Apo A1 inhibits lecithine cholesterol acyl
transferase
E. Apo B100 is part of LDL
2. Regarding about LL;
A. LDL contains hiher amo!nt of triacyllycerol compare" to
cholesterol
B. #ts f!nction is to carry cholesterol to the peripheral tiss!e only
C. $hey bin" to the cell s!rface membrane receptors %no&n as
ApoB100'ApoB48 receptors
D. #t is transforme" from VLDL after the process of cholesteryl
ester transfer protein &ith (DL
E. #t involves reverse cholesterol transport
!. Re"ating to chy"omicrons;
A. #t is synthesi)e" from the intestinal m!cosal cells
B. #t cannot bin" to apoB48 synthesi)e" from rE*
C. +ascent chylomicron receives apoC11 an" apoE from (DL
D. $he particle "ecrease in si)e an" increase in "ensity shortly
after lipoprotein lipase activity in the capillary &all
E. ApoE is not retaine" in the remnant chylomicron
4. Regarding R#V#RS# C$%L#S&#R%L &R'()%R&;
A. #t involves the effl!, of cholesterol from peripheral tiss!es to
(DL by ABCA 1 cassette protein &hich is A$- in"epen"ent
B. Esterification of cholesterol involves lecithin cholesterol
acyltransferase
C. $he phosphati"ylcholine of the (DL is transforme" into
lysophosphati"ylcholine &hich imme"iatly bin"s to the alb!min
D. (DL ma%es !p .0/ of apoA1
E. (DL carries cholesterol only from the bo"y0s tiss!es to the liver
*. Regarding SRB-1 (sca+enger receptor c"ass B type 1 )
A. #t s!pports the !pta%e of cholesteryl ester from (DL by the liver
B. #t facilitates the effl!, of !nesterifie" cholesterol
C. #t has hih e,pression in macrophaes pres!mably in
atherosclerosis
D. 1*B1 can interact only &ith (DL
E. #t is i"entifie" as o,iLDL receptor
,. -n the process o. atherosc"erosis
A. the o,i"i)e" LDL ca!ses "amae to the t!nica intima of the
bloo" vessel
B. 2onocytes are attracte" by chemoattractants to the en"othelial
cells to move into s!ben"otheli!m an" are transforme" into
macrophaes
C. (ih affinity receptors for LDL remains !nchane"
D. 2acrophaes cons!me e,cess o,i"i)e" LDL to become foam
cells
E. 3oam cells secrete roeth factors an" cyto%ines to stim!late
miration of smooth m!scle from t!nica intima to t!nica me"ia for
proliferation of smooth m!scle to pro"!ce collaen in ta%in !p
o,i"i)e" LDL as &ell to become foam cells
/. Regarding cho"estero" characteristics
A. #t is a str!ct!ral component of all cell membrane
B. #t is a prec!rsor to formation of l!cose
C. #t is a prec!rsor to formation of bile aci"s
D. #t is synthesi)e" from the liver itself
E. #t can serve as a carrier for lipoproteins
0. Regarding cho"estero" synthesis
A. All carbons for cholesterol are s!pplie" by acetate
B. +AD-( is involve" in the synthesis
C. (24 CoA synthase is the rate limitin en)yme for cholesterol
synthesis
D. #ns!lin ca!ses the activation of (24 CoA re"!ctase
E. 4l!caon ca!ses activation of (24 CoA re"!ctase
1. Re"ating to cho"estero" degradation5
A. $he sterol rin of cholesterol can be metaboli)e" into C67 an"
(70
B. $he conversion of cholesterol into cholic aci" is the re!late"
step to synthesis of bile aci" in the liver
C. $he ratio of lycine to ta!rine is lo&
D. $he mi,t!re of bile aci" an" bile salts is absorbe" primarily in
the ile!m via a +A B#LE 1AL$ C6$*A+1-6*$E*
E. Alb!min carries bile aci" in a non covalent comple, in the
bloo" bac% to the liver in enterohepatic circ!lation
12. Regarding de no+o synthesis o. .atty acid
A. 3irst step is the o,i"ation of pyr!vate to form mitochon"rial
acetyl coA
B. CoA of the acetyl coA can pass thro!h the inner mitochon"rial
membrane
C. Acetyl CoA carbo,ylase is activate" by citrate only
D. 3atty aci" synthase has an acyl carrier protein "omain &ith
covalently bin"e" 408phosphopenthamine
E. $he en" pro"!ct forme" is palmitate
11. -n the regu"ation o. acety" Co' carbo3y"ase
A. #ns!lin activates protein phosphatase to activate acetyl coA
carbo,ylase
B. #ns!lin ca!ses phosphorylation of the acetyl coA carbo,ylase
C. ADE+61#+E 26+6-(61-A($E DE-E+DE+$ 9#+A1E
:A2-8%inase; is re!late" both allosterically an" covalently
D. 4l!caon activate acetyl coA carbo,ylase
E. 2alonyl coA is the en" pro"!ct
12. -n the desaturation o. .atty acid chains4
A. #ts en)ymes are present in the rE*
B. #t involves a""ition of cis "o!ble bon"s
C. (!man have carbon 45 < an" = "esat!rases only
D. 3irst "o!ble bon" is inserte" bet&een carbon= an" 10
E. (!mans lac% the ability to intro"!ce "o!ble bon" from carbon
10 to the &8en" of chain.
Ans&ers>
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4. 3 $ $ $ 3
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@. $ $ 3 $ 3
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8. $ $ 3 $ 3
=. 3 $ 3 $ $
10. 3 3 3 $ $
11. $ 3 $ 3 $
17. 3 $ 3 $ $
Biochemistry Lec *-0
1) &he .ormation o. .atty acids .rom hormone sensiti+e "ipase
en5yme are .o""o6ed as7
A. A"ipose tiss!e is the store for fatty aci"s
B. (ih l!cose level stim!lates the lipase en)yme
C. c A2- me"iate" casca"e t!rns on fatty aci" synthesis
D. 4l!caon activates protein phosphatase to convert en)yme bac%
into inactive form
E. #ns!lin ca!se inhibition of fatty aci" synthesis
2) Regarding transport o. .atty acid .rom cytoso" to
mitochondria;
A. 3atty aci" is activate" by malonyl coA in the cytosol
B. $he transport carrier for activate" fatty aci" is %no&n as
carnithine sh!ttle
C. $his process involves t&o carnithine palmitoyl transferase
en)ymes in each mitochon"rial membrane respectively
D. 2alonyl coA activates carnithine acyltransferase81 to allo&
entry of fatty acyl ro!ps into the matri,
E. 4enetic "efect in carnithine sh!ttle res!lts in inability of
"era"ation of fatty aci"
!) Regrading .atty acid B- o3idation8 it in+o"+es the .o""o6ing
en5ymes7
A. 3AD "epen"ent acyl coA "ehy"roenase
B. Enoyl Co A hy"ratase
C. acetyl carbo,ylase
D. B8 hy"ro,yacyl Co A "ehy"roenase
E. malonyl Co A transferase
4)Regarding 9etone bodies4
A. $hey are in nee" of transportation from the bloo" to the
-eripherals tisAes by albAmina or lipoproteins
B. -eripheral tiss!es have thiophorase en)yme &hich activates
acetoacetate to acetyl coA
C. $hey are pro"!ce" in the a"ipose tiss!e
D. $hey are !se" as alternative enery so!rce by the s%eletal
m!scle5 car"iac m!scle an" renal corte,
E. $hey are "erive" from acetyl coA pro"!ce" from fatty aci"
o,i"ation
*) Regarding biosynthesis o. triacy"g"ycero"5
A. Activation of fatty aci" is prec!rsor of $A4
B. 1o!rce of lycerol ? phosphate from liver only
C. $here is formation of lysophosphati"ic aci" after phosphati"ic
aci"
D. 4lycerol ? phosphate is "erive" from lycolysis an" free
4lycerol
E. 78 monacyllycerol is synthesi)e" from intestinal m!cosal only
,) &he .o""o6ing are part o. g"ycerophospho"ipids8
A. -hosphati"yllycerol
B. Car"iolipin
C. Lecithin
D. -latelet activatin factor
E. 1phinomyelin
/) Regarding synthesis o. phospho"ipids5
A. $he prec!rsor is the phosphati"ic aci"
B. -hosphati"ylcholine is forme" from combination of CD-
choline an" "iacyllycerol
C. -hosphati"yllycerol is forme" from combination of CD-
"ialycerol an" lycerol
D. -hosphocholine is converte" into CD-8 Choline via cyti"yl
phosphocholine transferase
E. Car"iolipin synthesis reB!ires the formation of CD- C
Diacyllycerol from phosphati"ic aci" via C$- !tili)ation
0) Re"ating to .atty "i+er8 there is
A. #ncrease" plasma free fatty aci"
B. #mpaire" synthesis of VLDL
C. *e"!ce" +AD(>+AD ratio
D. #ncrease" choline synthesis
E. -resence of chloroform an" CCL4
1) Re"ating to respiratory distress syndrome4 it is due to
A. car"iolipin "eficiency
B. -!lmonary s!rfactants "eficiency
C. #mmat!rity of the l!ns
D. #nflation of l!ns
E. (eart attac%
12) Regarding p"asma en5ymes8
A. 3!nctional en)ymes are present at all times an" present
physioloical f!nction in the bloo"
B. 3!nctional en)ymes are commonly synthesi)e" in the tiss!es
C. +on f!nctional en)ymes are those present in the e,ocrine
secretions as &ell as intracell!lar en)ymes release" into plasma by
normal ro!tine "estr!ction of *BCs5 le!%ocytes an" other cells
D. +on C f!nctional en)yme are release" "!rin accelerate" cell
"eath
E. Contains a variety of en)ymes that are normally present at
"ifferent concentrations
11) Regarding isoen5ymes8
A. $hey have the same catalytic activity
B. $hey have same 9m val!e
C. $hey have "ifferent isoelectric points
D. Lactate Dehy"roenase is a tetramer compose" of t&o
s!b!nits; 2 an" (
E. $he pre"ominant form of Lactate "ehy"roenase in s%eletal
m!scle an" car"iac m!scle are 24 an" (4 respectively.
12) &he .o""o6ing are the c"assi.ication o. hyper"ipidemia>
A. $ype 1 is hih in cholesterol
B. $ype 7a is "!e to m!tate" LDL receptor res!ltin in increase"
ser!m cholesterol
C. $ype 7b is ca!se" by overpro"!ction of VLDL by the liver
D. $ype ? ca!ses ,anthomas an" accelerate" vasc!lar "isease by
mi""le ae.
E. $ype < is "!e to increase" in ser!m triacyllcerol only.
'ns6ers7
1; $ 3 3 3 $
7; 3 $ $ 3 $
?; $ $ 3 $ 3
4; 3 $ 3 $ $
<; $ 3 3 $ $
@; $ $ $ 3 3
.; $ $ $ $ $
8; $ $ 3 3 $
=; $ $ $ 3 3
10; $ 3 $ $ 3
11; $ 3 $ $ $
17; 3 $ $ $ 3