CELL BIOLOGY LABORATORY

LAB 5: BLOOD CELL AND CENTRIFUGATION

Purpose: Learn erythrocytes, leukocytes, thrombocytes meaning and to observe and compare the
differences between coagulation and anti coagulation tube result.

Introduction:

A blood cell, also called a hematocyte, is a cell produced by hematopoiesis and

normally found in blood. In mammals, these fall into three general categories:
Red blood cells Erythrocytes
White blood cells Leukocytes
Platelets Thrombocytes.
Together, these three kinds of blood cells add up to a total 45% of the blood tissue by volume, with
the remaining 55% of the volume composed of plasma, the liquid component of blood.[1] This
volume percentage (e.g., 45%) of cells to total volume is called hematocrit, determined by centrifuge
or flow cytometry. Hemoglobin (the main component of red blood cells) is an iron-containing protein
that facilitates transportation of oxygen from the lungs to tissues and carbon dioxide from tissues to
the lungs.
Blood fractionation is the process of fractionating whole blood, or separating it into its component
parts. This is typically done by centrifuging the blood.
The resulting components are:
a clear solution of blood plasma in the upper phase (which can be separated into its own fractions,
see Blood plasma fractionation),
the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets in the
middle, and erythrocytes (red blood cells) at the bottom of the centrifuge tube.
Serum separation tubes (SSTs) are tubes used in phlebotomy containing a silicone gel; when
centrifuged the silicone gel forms a layer on top of the buffy coat, allowing the blood plasma to be
removed more effectively for testing and related purposes.

Metarial:

Blood(~60 ml)
Isolated solution (%9 NaCl)
Tube
Centrifugal device
Slide
Cover slip
Dropper
Microscope

Method:

Withdraw blood into a syringe and place in a tube.

Put 9 ml isotonic solution (%9 NaCl) in tube
Place in tube into a centrifuge and spin for about 10 minutes
Components of blood seperate during centrifugation to reveal plasma, buffy coat, and
erythrocytes.
Take 1 ml in blood sample via dropper
Put slide blood sample and observe under the microscope.
We apply this method for anti coagulation tube

Result and Discussion: In this lab we blood fractionation with centrifugal device. Blood contain
different types of cells, for example red blood cells (erythrocytes), which transport oxygen to all
tissues in the body and white blood cells (leukocytes), which are part of the immune system. In this
lab we used two type tube.First tube have coagulation but second tube dont have coagulation
meterial.We take sample and put slide then we observed under the microscope. I observed
eryhroctes, leukoctes and nucleus both them but there were differences between the two samples
because coaglation tube dilluted blood and this part more solid then anti coaglation tube.
For example coagulation tubes eryhrocytes closer to anti coaglation tubes eryhroctes. This also
applies to other materials (leukoctes, nucleus).This is microscopic observed result. My macroscobic
observed was two tube had two different view.

The left photo had coaglation and we observed sequential and orderly layer but we dont observe
right photo because coaglation solidify of blood therefore we observed different view.