1, June, 2009: 17 - 32
Original Article
ABSTRACT
Introduction: Many products had been used as chemical peeling agents to renew thin skin. Retinoic, Glycolic and
Salicylic acids were used in many dermatological diseases with hyperkeratinization and/or hyperpigmentation.
Aim of the Work: To evaluate and compare the effects of these acids on the thin skin of adult female guinea pig.
Materials and Methods: Twenty animals were used and were divided into four equal groups. Group I served as the
control group, whereas the other treated groups were topically applicated daily on shaved area of back skin with
Retinoic (0.05%), Glycolic (12%) and Salicylic (10%) acids in Groups II, III and IV, respectively. Thin skin specimens
were processed for light and transmission electron microscopic studies. Morphometric and statistical studies were
done.
Results: Groups II, III and IV showed high significant increase in mean thickness of epidermal nucleated keratinocytes as
compared to Control group. Group II showed proliferation hyperkeratosis and acanthosis and shrinkage of the sebaceous
glands with decreased sebum production. Group III showed skin peeling by removing superficial layers of epidermis,
cytoplasmic and nuclear degeneration with disruption of intercellular junctions and degeneration in melanocytes with
marked decrease of melanin. Both Retinoic and Glycolic acids apparently increased the production of collagen and
elastic fibers as compared to control. Group IV showed skin peeling mainly by direct action on intercellular cement
substance and partially by inducing proliferation hyperkertosis.
Conclusion: Both Retinoic and Glycolic acids showed complementary actions in treatment of hyperpigmentation and
as chemical peeling agents. Salicylic acid also is a peeling agent, but its effects could be covered by either Retinoic or
Glycolic acids.
Recommendation: It is recommended to use combination of both Retinoic and Glycolic acids to give better effects on
various skin disorders with hyperkeratinization and hyperpigmentation.
E-mail: abirmohsen@yahoo.com
INTRODUCTION
Some
authors4 stated that photoaging
pathophysiology is characterized clinically by wrinkles,
mottled pigmentation, rough skin and loss of skin tone
with histologic changes in the dermal connective tissue.
Other authors5 stated that the use of topical retinoids
were capable of repairing photoaged skin and treating
intrinsically aged skin. Moreover, some investigators6
noticed that topical retinoids were considered the firstline therapy in the treatment of acne vulgaris. Isotretinoin
(retinoids) appeared to be the most potent agent that
affects all the pathogenic features of acne, which resulted
from the interplay of 4 factors: Increased production of
sebum by the sebaceous gland, altered keratinization of
follicular keratinocytes, activity of Propionibacterium
3 (1128-2009)
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Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
Alpha hydroxy acids (AHA), a group of naturallyoccurring compounds, have the potential to improve
a variety of skin disorders11. Some authors12 stated that
AHAs are derived from food sources; although many of
these compounds are available, glycolic acid has been the
most widely used. Hydroxy acids had been extensively
used in cosmetic and dermatologic formulations because
of their satisfactory results in terms of maintaining a
young and healthy skin and in recovering aged skin13.
1.
2.
Mean
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RESULTS
Light and Electron Microscopic Results:
Group I (Control group):
19
Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
Groups
Group I (Control)
30.575.69
160.595.43
86.4613.53
117.4011.11
SD = standard deviation.
180
160
140
120
100
80
60
40
20
0
Group I
C ontrol
Group II
Retinoic
acid
Group III
Glycolic
acid
Group IV
Salicylic
acid
20
Group I
(Control)
Group II
(Retinoic acid)
Group III
(Glycolic acid)
Group IV
(Salicylic acid)
(J) code
Mean Difference
(I-J)
Significance
Group II
-130.02200
p<0.001 (H.S.)
Group III
-55.88800
p<0.001 (H.S.)
Group IV
-86.83300
p<0.001 (H.S.)
Group I
130.02200
p<0.001 (H.S.)
Group III
74.13400
p<0.001 (H.S.)
Group IV
43.18900
p<0.001 (H.S.)
Group I
55.88800
p<0.001 (H.S.)
Group II
-74.13400
p<0.001 (H.S.)
Group IV
-30.94500
p<0.001 (H.S.)
Group I
86.83300
p<0.001 (H.S.)
Group II
-43.18900
p<0.001 (H.S.)
Group III
30.94500
p<0.001 (H.S.)
21
Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
22
23
Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
24
25
Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
26
DISCUSSION
The epidermis of thin skin sections of animals of
Group II showed that topical application of Retinoic acid
increased the thickness of viable keratinocytes forming
prominent rete pegs. This was proved by morphometric
and statistical study where the mean thickness of
nucleated epidermal keratinocytes was high significantly
increased (p<0.001) as compared to either Control,
Glycolic acid or Salicylic acid Groups. It measured the
highest mean thickness. This coincided with the results
of some investigators20,21. They found that Retinoic
acid induced keratinocyte proliferation in aged and
photodamaged skin. Coinciding in the present study,
the epidermis of Group II showed enhanced epithelial
proliferation and the nuclei of cells of stratum spinosum
appeared vesicular and exhibited markedly obvious
mitotic figures. This was observed by some scientists22,23.
27
Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
28
29
Comparative Study on the Effect of Retinoic Acid, Glycolic Acid and Salicylic Acid on the Thin Skin
14.
15.
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