BIO3V20 General Microbiology (5 credits)

Spring 2006 Schedule (version 1.0)

TR 2:00-3:15 pm FO3.616 (lecture)
TR 3:30-6:00 pm MP2.202 (lab)

Dr. Ernie Hannig TAs:

Office: FO 3.714 Swati Ghatpandi
Phone: 972-883-2505 Sirisha Kodeboyina
email hannig@utdallas.edu Jennifer Morris
Office hours: by appointment

Required Textbooks:
Madigan M.T., Martinko, J.M., and Parker, J. (2003) Brock Biology of Microorganisms, 10th ed. Prentice Hall/Pearson
Education, Inc., Upper Saddle River, NJ. [ISBN 0-13-066271-2] (lecture)
Note: (2006) 11 th edition [ISBN 0-13-144329-1] is equally suitable.

Cappuccino. J.G., and Sherman, N. (2005) Microbiology: A Laboratory Manual, 7th ed. Benjamin Cummings [ISBN 0-8053-
2836-X] (lab)

Recommended (atlas)
Alexander, S.K., and Strete, D. (2001) Microbiology: A Photographic Atlas for the Laboratory. Benjamin Cummings [ISBN 0-
8053-2732-0]

Recommended (highly) Reading

De Kruif, P. Microbe Hunters.
This book was originally published in 1926, but reprint editions are available.

Date Lecture Chapter (Brock, 10 th Lab Chapter

Jan. 10 (Tues) Welcome
Jan. 12 (Thurs.) Microorganisms and
Microbiology
An Overview of
Microbial life
Jan. 17 (Tues.) Macromolecules
Cell Structure/Function
Jan. 19 (Thurs.) Cell Structure/Function
Jan. 24 (Tues.) Cell Structure/Function
(Cappuccino and
edition) Note: 11 th
edition is essentially Sherman, 7th
identical for these edition) [6th edition
chapters chapters in brackets
if different]
Ch. 1 (21 pgs) Seminar 4 pm. Dr. Gireesh
Rajashekara
Ch. 2 (17 pgs) "Identification of Brucella
melitensis virulence genes"
Ch. 3 (17 pgs) (this
should be a review)
Ch. 4 (47 pgs)
Ch. 4 (47 pgs) Seminar 4 pm.
Dr. Scott Landfear. "An
Essential Role for Glucose
Transporters in the Life
Cycle of Leishmania
Parasites"
Ch. 4 (47 pgs)

Jan. 26(Thurs.) Nutrition, Culture, and Ch. 5 (35 pgs) + Introduction pgs. xii-xvi;
Metabolism I handouts Lab Safety

Jan. 31(Tues.) Nutrition, Culture, and Ch. 5 (35 pgs) + Microscopy: Measurements Exp. 4; 6, 8, 9
Metabolism II handouts of microorganisms
Bacterial staining:
preparation, simple
staining

Feb. 2 (Thurs.) Nutrition, Culture, and Ch. 5 (35 pgs) + Continue and finish Exp. 4;
Metabolism III handouts 6, 8, 9
Feb.7 (Tues.) Microbial Growth I Ch. 6 (30 pgs) Negative stain; Gram stain Exp. 10, 11

Feb. 9 (Thurs.) Microbial Growth II Ch. 6 (30 pgs) Acid-fast stain Exp.12

Feb. 14 (Tues.) Exam I Ch. 1-6 Cultivation and cultural Exp. 1-3
characteristics of
microorganisms
Feb. 16 (Thurs.) Principles of Microbial Ch. 7 (40 pgs) Finish Exp. 1-3
Molecular Biology I
Feb. 21 (Tues.) Principles of Microbial Ch. 7 (40 pgs) Bacterial growth: physical Exp. 18-20
Molecular Biology II factors (oxygen) [17-19]
Serial dilution: viable cells
Feb. 23 (Thurs.) Regulation of Gene Ch. 8 (25 pgs) Finish Exp. 18-20
Expression

Feb. 28 (Tues.) Bacterial Genetics I Ch. 10 Differential and selective Exp. 15, 22,
media; 25B, 25C
Biochemical tests [14, 21, 24B,
(extracellular enzyme 24C]
activities for starch, lipid,
and protein hydrolysis);
IMViC tests (MR/VP)
Mar. 2 (Thurs.) Bacterial Genetics II Ch. 10 Finish Exp. 15, 22, 25B,
25C
Mar. 7 (Tues.) Spring Break
Mar. 9 (Thurs.) Spring Break
Mar. 14 (Tues.) Microbial Evolution and Ch. 11 (30 pgs) Biochemical tests Exp. 23, 24,
Systemics (carbohydrate utilization; 25A, 25D, 32
indole production; citrate [22, 23, 24A,
and amino acid utilization 24D, 31]
Mar. 16 (Thurs.) Essentials of Virology I Ch. 9 (33 pgs) Biochemical tests (litmus Exp.28, 30, 31
milk, catalase, oxidase) [27, 29, 30]

Mar. 21 (Tues.) Exam II Ch. 7, 8, 10, 11 Biochemical tests (H2S, Exp. 26, 27, 29;
(note Ch. 9 is not urease, nitrate reduction); start 33 (mixed
included) start mixed unknowns unknowns)
[25, 26, 28, 32]
Mar. 23 (Thurs.) Essentials of Virology II Ch. 9 (33 pgs) Finish Exp. 26, 27, 29;
continue exp. 33 (weekly until
completed)

Mar. 28 (Tues.) Bacterial, Plant, and Chapter 16 (27 pgs) Bacterial viruses: isolation, Exp. 39 [38]
Animal Viruses I cultivation, and enumeration
Mar. 30 (Thurs.) Bacterial, Plant, and Chapter 16 (27 pgs) Parasitic protozoa Exp. 35
Animal Viruses II [34]

Apr. 4 (Tues.) Microbial Growth Control Ch. 20 (32 pgs) Growth control: physical 41, 44, 46 [40,
I agents (moist heat); Growth 43, 45]
control: chemical agents
(antibiotics and other
chemical agents)
Apr. 6 (Thurs.) Microbial Growth Control Ch. 20 (32 pgs) Finish Exp. 41, 44, 46
II

Apr. 11 (Tues.) Human-Microbe Ch. 21 (28 pgs) Mycology Exp. 36

Interactions I [35]

Apr. 13 (Thurs.) Human-Microbe Ch. 21 (28 pgs) Finish Exp. 36

Interactions II
Apr. 18 (Tues.) Immunology Ch. 22 (32 pgs) Work on unknowns
Apr. 20 (Thurs.) Catch-up Work on unknowns
 Apr. 25 (Tues.) Final Exam Ch. 9, 16, 20, 21, 22
Spring Break: March 6-11
Last day of class: April 24
Exam week: April 25-May 1