Experiment Report of BioChemistry 1

I.

TITLE OF EXPERIMENT

The Effect of pH and Enzyme Concentration Cocerning Enzyme Amylase Activity

II.

DATE OF EXPERIMENT

th

December 5 2014
III.

END OF EXPERIMENT

December 5th 2014

IV.

PURPOSE OF EXPERIMENT

Proven that pH and enzyme concentration is affected in enzyme amylase activity

V.

BASIC THEORY

Experiment Report of BioChemistry 1

VI.

TOOLS AND MATERIALS

Tools :
1.

Alluminium test tube rack

(1 item)

2.

Test tube pyrex

(12 items)

3.

Funnel glass 5mm

(1 item)

4.

Measured flask 50ml pyrex

(2 item)

5.

Volume pippete pyrex

(1 item)

6.

Graduated cylinder 10ml herma (1 item)

7.

Spatula

(1 item)

8.

Beaker glass 100ml duran

(5 items)

9.

Drop pippete

(20 items)

10. Label paper

(1 sheet)

11. Spectrofotometry UV-VIS

(1 item)

12. Tissue

(Conditionally)

Materials :
1.

Salive as a source of amylase

2.

Starch solution 0.4mg/ml

3.

Starch solution 0.4mg/ml dilluted in kinds of pH (1,3,5,7 and 9)

4.

Iodium solution

5.

Aquadest

Experiment Report of BioChemistry 1

VII.

PROCEDURE

Experiment Report of BioChemistry 1

IX.

DATA ANALYSIS

Experiment Report of BioChemistry 1

REFFERENCES

Agustini, Rudiana dkk. 1993. Petunjuk Praktikum Biokimia. Surabaya: Unipress;

IKIP
Carey, A Francis. Organic Chemistry 4th Ed. Ebook: University of Virginia
Lehninger, Albert L. 1990. Dasar-dasar Biokimia Jilid 1. Terjemahan.
Jakarta;:Erlangga
Nelson, D.L. Lehninger. 1990. Principle of Biochemistry 4th. New York:
W.H.Freeman
Tim Penyusun. 2012. Petunjuk Praktikum Biokimia. Surabaya: Unesa Press

Experiment Report of BioChemistry 1

ANSWER OF QUESTION

Experiment Report of BioChemistry 1

CALCULATED ATTACHMENT
The Effect of Concentration
WL blanco: 0,052

Absorbance (WL

Concentration

WL

100x

0,046

0,006

200x

0,048

0,004

300x

0,049

0,003

400x

0,050

0,002

500x

0,055

-0,003

blanco-WL sample)

Graph:

Concentration Graph)
0.008

0.006

Absorbance

0.004

0.002
y = -0.002x + 0.0084
R = 0.885

0
100x

200x

300x

-0.002

-0.004

Concentration

400x

500x

Experiment Report of BioChemistry 1

The pH Optimum
WL blanco: 0,054

Absorbance (WL

pH

WL

0,052

0,002

0,049

0,005

0,048

0,006

0,052

0,02

0,055

-0,001

blanko-WL sample)

Graph:

pH Graph
0.025
0.02

Absorbance

0.015
0.01
0.005
0
0
-0.005

6
pH

10

Experiment Report of BioChemistry 1

PICTURE ATTACHEMENT

No

Picture

Explanation

1
Saliva dilluted with aquadest

Blanco solution : Amylum + I2 is light

brown

Blanco solution : Amylum + I2 +

aquadest is light yellow

For pH experiment : Amylum +

sample is colorless

Amylum + sample + I2 is light

brown color

Experiment Report of BioChemistry 1

Amylum + sample + I2 + aquadest

is light yellow color

2.
For concentration experiment :
as a sample solution
Saliva + aqadest : colorless

Sample + amylum is colorless

solution

Sample + amylum + I2 is orange

color

Experiment Report of BioChemistry 1

Incubation process

After incubation the solution still

orange color

Sample + amylum + I2 + aquadest

is light yellow color

Read the absorbance used

spectrofotometry UV-VIS

Experiment Report of BioChemistry 1