Effectivity of Several Antiacne Facial Wash

Against Propionibacterium acnes

Nikken Rima Oktavia, Intan Keumala Dewi, Endah Wulandari
Medical Students in Medical Education and Health Sciences Faculty of UIN Syarif Hidayatullah
Research Supervisor in Medical Education and Health Sciences Facilty of UIN Syarif Hidayatullah

Background
Acne vulgaris is a skin problem that affects nearly all adolescents and
young adults with varying degrees of severity.
One of the bacteria that play a role in the pathogenesis of acne vulgaris is
Propionibacterium acnes.
Clean your face with sfacial washis one way of preventing acne vulgaris.
Antiacne facial wash works with a variety of mechanisms, namely lifting
debris, sweat, bacteria, and excess fats on the skin in the form of an
emulsion.
Currently circulating antiacne facial wash that contains antibacterial
substances.

The result from studies that was conducted in vitro by disc

diffusion method, after incubation for 24 h obtained antiacne
facial wash that has the greatest inhibitory effect in inhibiting
the growth of bacteria Propionibacterium acnes, antiacne facial
wash BR (triclosan and lauric acid), then SA (triclosan and lauric
acid) and PD (salicylic acid and lauric acid).
Kruskall walis test p = 0.014 (p <0.05) there is a significant
difference between antiacne facial wash on inhibit the growth
of Propionibacterium acnes in vitro.

35
SA : Triclosan,
lauric acid

Objective
The purpose of this study to determine the effectiveness of several
antiacne facial wash against Propionibacterium acnes growth.

Method
The study was conducted in vitro using an experimental design
with disc diffusion technique. Then measuring the inhibitory
zone.

Average diameter of
Inhibition zone (mm)

30

PD : Salicylic
acid, lauric
acid

25
20

BR : Triclosan,
lauric acid

15

CC : Salycilic
acid

10

(+):clyndamycin
10ug/mL
(-): ethanol 96%

Result
The result from studies that was conducted in vitro by disc
diffusion method, after incubation for 14 h obtained antiacne
facial wash that has the greatest inhibitory effect in inhibiting
the growth of bacteria Propionibacterium acnes, antiacne facial
wash BR (triclosan and lauric acid), followed by CC (salicylic
acid), SA (triclosan and lauric acid) and PD (salicylic acid and
lauric acid).
Kruskall walis test p = 0.005 (p <0.05) there is a significant
difference between antiacne facial wash on inhibit the growth
of Propionibacterium acnes in vitro.

Di

0
SA PD BR CC (+) (-)

In this study, pH is measured on each of the antiacne facial wash

to determine whether the degree of acidity of antiacne facial
wash that affect the bacterial inhibition zone.
Pearson correlative test p = 0.462 (p <0.05) no relationship
between the pH value of the inhibition zone formed

Conclusion
35
SA : Triclosan,
lauric acid

Average Diameter of
Inhibition zone (mm)

30

PD : Salicylic
acid, lauric
acid

25
20

BR : Triclosan,
lauric acid

15

CC : Salycilic
acid

10

(+):clyndamycin
10ug/mL
(-): ethanol 96%

5
0
SA PD BR CC (+) (-)

Several brands of antiacne facial wash which are tested, antiacne facial
wash that has the greatest inhibitory effect on the growth of
Propionibacterium acnes is antiacne facial wash containing antibacterial
agents triclosan and lauric acid

Reference
1. Krowchuk DP. Managing adolescent acne: a guide for pediatricians.
Pediatr Rev. 2005:250-261.
2. C. Beylot, N. Auffret, et al. Propionibacterium acnes: an update on its
role in the pathogenesis of acnes. European Academy of Dermatology
and Venerology Journal. 2013.