Description

DepositedName:BacillusstearothermophilusDonk
ProductDescription:

ProductSheet

Geobacillus
stearothermophilus
(ATCC7953)

ThisstrainisrecommendedbyATCC foruseinthetestsdescribedinmilitaryspecificationMILS36586A
andU.S.Pharmacopeia,23rdrev.,<1211>,pp.19761981,1995whereonlythetaxonisspecified.Steam
andgassterilizationcontrol.

Propagation
Medium
Medium3:Nutrientagarornutrientbroth

PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

GrowthConditions
Temperature:55C
Atmosphere:Aerobic
PropagationProcedure
1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#3broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
1.0mLpipette.Rehydratetheentirepellet.
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
4. Useseveraldropsofthesuspensiontoinoculatea#3agarslantand/orplate.
5. Incubatethetubesandplateat55Cfor24to48hours.

Notes

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Geobacillusstearothermophilus
(ATCC7953)

Growthislightmoderateinbrothandheavyonagar.

Colonialvariationmaybeobservedandistypicalforthisstrain.Twocolonytypesobserved:a)entire,
circular,smooth,opaque,dull,andraisedandb)slightlyirregular,smooth,translucent,umbonate,glistening,
andentire.

Depositorstatedthatthisstraingrowsonagarat35C,butthiswasnotverifiedbyATCClaboratories.

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

References
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page1of2

TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

Disclaimers
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas

ProductSheet

beenconfirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
reasonableeffortismadetoinsureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

Geobacillus
stearothermophilus
(ATCC7953)
PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Geobacillusstearothermophilus
(ATCC7953)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page2of2

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2013.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[12/09]