Kits Magpix Millipore

Bring Your
Biomarkers to Life.
The #1 best seller for multiplexing using
Luminex technology
Analyte Quarterly, Vol. 2 2014

Immunoassays & MILLIPLEX
Bone Metabolism
Cancer Biomarkers
Cardiovascular Disease
Immunology/
Immune Response
Intracellular
Cell Signaling
Cellular Metabolism
Metabolism/
Endocrinology
Neuroscience
Toxicity
map
Kits:
NEW PA NEL S
The Latest in
Multiplexing & Single
Protein Detection
Discover the Latest in Innovative and Unique Multiplex

and Single Protein Assay Kits and Luminex Instruments
NEW: MILLIPLEX map
and Single Protein Detection
For Research Use Only.
MILLIPLEX
MILLIPLEX
map
Kits
Species
Catalogue No.
Species
Catalogue No.
Adipokine
Rat
Coming Soon!
Sepsis Panel 4
Human
HSP4MAG-63K
Adipocyte
Rat
Coming Soon!
Sepsis Panel 5
Human
HSP5MAG-63K
Adiponectin
Rat
Coming Soon!
Metabolic Hormone
Feline
FMHMAG-20K
Kidney Toxicity Panel 2
Canine
Coming Soon!
CVD Panel 2
Mouse
MCVD2MAG-77K
Liver Injury
Rat
Coming Soon!
Cytokine/Chemokine Panel 2
Primate
PRCYTMAG2-40K
Cytokine/Chemokine Panel 2,
24-Plex Premix
Primate
PRCYTMAG40K-PX24
ELISA Kits
Species
Cytokine/Chemokine Panel 2,
25-Plex Premix
Primate
PRCYTMAG40K-PX24
C-Peptide
Canine
Coming Soon!
PGF2
Human
Coming Soon!
Soluble Cytokine Receptor
Human
HSCRMAG-32K
PGE2
Human
Coming Soon!
Soluble Cytokine Receptor

14-Plex Premix
Human
HSCRMAG32KPX14
STAT3 Phospho/Total 2 Plex
Human
48-623MAG
GyroMark HT Kits
Species
Vascular Injury Panel 1
Rat
RV1MAG-26K
Insulin
Human
Coming Soon!
Rat
RV2MAG-26K
Glucagon
Human
Coming Soon!
Rat
RV3MAG-26K
-2-Microglobulin
Rat
Coming Soon!
Kidney Injury Panel 1
Human
HKI1MAG-99K
Cystatin C
Rat
Coming Soon!
Human
HKI2MAG-99K
Human
HKI3MAG-99K
Human
HKI4MAG-99K
Human
HKI5MAG-99K
Human
HKI6MAG-99K
Mouse
MKI1MAG-94K
Mouse
MKI2MAG-94K
Kidney Toxicity Expanded

Panel 1
Canine
CKT1MAG-97K
GyroMark HT Kits
Species
Catalogue No.
Clusterin
Rat
GLP-1, Active
Multispecies
GYGLP1A-35K
GLP-1, Total
Multispecies
GYGLP1T-36K
KIM-1
Rat
GYRKIM1-20K
Insulin
Rat/
Mouse
map
Kits
Coming Soon
merckmillipore.com/bmia
GYRCLU-37K
GYRMI-13K
Catalogue No.
Catalogue No.
Online Kit Ordering Is Faster

& Easier!
Order your MILLIPEX map panels and kits online.

Streamlined catalogue search
Dynamic kit design based on species, panel type and
analyte compatibility
Fully automated with minimal computer think time
for rapid, accurate ordering
Instant pricing
SPOTLIGHT
MILLIPLEX MAP New Product

Spotlight
MILLIPLEX map Mouse Kidney Injury Magnetic Bead Panel 1 Using
Luminex xMAP Technology
(Cat. No. MKI1MAG-94K)
Compared to the traditionally used rats, using a mouse

model of kidney injury or drug-induced kidney toxicity
can save time and money. Based on the predictive
urinary biomarkers approved by the FDA and EMA for
acute nephrotoxicity in preclinical rat studies, we have
developed the MILLIPLEX map Mouse Kidney Injury
Magnetic Bead Panels 1 and 2, which detect 11 specific
and relevant kidney injury biomarkers in urine samples.
IP-10
5
0
24 h
48 h
Renin
10
5
0
24 h
48 h
48 h
72 h
15
5
0
Vehicle
Cisplatin
10
*
24 h
TIMP-1
48 h
72 h
VEGF
Vehicle
Cisplatin
6
4
2
24 h
KIM-1
20
72 h
Vehicle
Cisplatin
10
ng/mg uCr
ng/mg uCr
5
0
Vehicle
Cisplatin
10
72 h
15
0.25
ng/mg uCr
10
15
ng/mg uCr
ng/mg uCr
Vehicle
Cisplatin
ng/mg uCr
-2-Microglobulin
15
Here, we show a subset of the multiplex assay

data presented at the 2014 Society of Toxicology
Annual Meeting, in our poster, "Novel Kidney Injury
Biomarker Panels To Detect Nephrotoxicity in
Mouse Models1. In this model of drug-induced
tubular toxicity, Renin, KIM-1, TIMP-1, VEGF and IP-10
levels reached their peaks in 72 hours after Cisplatin
administration. No significant change was observed in
-2-Microglobulin levels (Figure 1).
0.25
Vehicle
Cisplatin
0.15
0.10
0.05
24 h
48 h
72 h
0.00
24 h
48 h
72 h
Figure 1. Protein Biomarkers in Cisplatin-Induced Kidney Injury Mouse Model.

Urine samples were collected from BALB/C male mice (n=4) injected with one dose of Cisplatin (20 mg/kg i.p.) at 24, 48 and 72 hours
from both vehicle- and drug-treated groups (n=4 for both groups) and analyzed at a dilution of 1:25 using MILLIPLEX map Kidney Injury
Panel 1. Data were normalized to urine creatinine concentrations for data analysis.
* indicates significant difference between the groups with p <0.05.
1. Merck Millipore Literature Number PS6113ENEU.
SPOTLIGHT
Bring your biomarkers to life.

GyroMark HT Assays
Introducing new, fully qualified kits for the
Gyrolab platform!
Kit
Species
Clusterin
Rat
GLP-1, Active
Catalogue No.
GYRCLU-37K
NEW!
Multi-species
GYGLP1A-35K
NEW!
GLP-1, Total
Multi-species
GYGLP1T-36K
NEW!
KIM-1
Rat
GYRKIM1-20K
NEW!
Insulin
Rat/Mouse
GYRMI-13K
NEW!
Insulin
Human
Coming Soon!
Glucagon
Human
Coming Soon!
-2-Microglobulin
Rat
Coming Soon!
Cystatin C
Rat
Coming Soon!
We build custom kits to meet your

specific needs!
Fully qualified, robust and reproducible assays

High correlation to ELISAs and Luminex assays
Order specific lots of reagents to minimize
variation and lot-to-lot drift
Offthe-shelf kits including all the components
to run an assay
ELISA (ng/mL)
Advantages of using Merck Millipore

off-the-shelf or custom-made
assays, compared to developing and
validating your own Gyrolab assays:
Rat/Mouse Insulin
3.5
3
2.5
2
1.5
y = 1.3302x - 0.031
R = 0.9787
1
0.5
0
0.5
1.5
2.5
Gyros (ng/mL)
High correlation to ELISA data.

Excellent correlation (r=0.98) between insulin
levels measured by GyroMark HT Rat/Mouse
Insulin Assay (Cat. No. GYRMI-13K) for the
Gyrolab xP Workstation and Merck Millipores
Rat/Mouse Insulin ELISA assay (Cat. No.
EZRMI-13K). Sample volume was 1,000 nL and
samples were diluted 1:2.
Merck Millipore has over 25 years experience in

kit development
GyroMark HT Kitseverything you need in one, easy-to-use format:

Buffers (specifically made for the Gyrolab platform)
Standards (recombinant proteins validated to World Health Organization standards, when available)
Quality Controls (low, middle and high concentration values to confirm kit performance)
Capture and Detection Antibody Pairs (selected for optimal performance)
Preloaded CD (produced by Gyros)
2
MILLIPLEX
map
CIRCULATING
Bring your biomarkers to life with

simultaneous multianalyte detection.
The broadest selection of analytes across a wide range of disease states
All the components and reagents you need to detect multiple analytes simultaneously
Quality controls provided to qualify assay performance
Analytically validated panels yielding consistent analyte profiles within panels
Comparison of standard & QC lots to a reference lot to ensure lot-to-lot consistency
Panels meet stringent manufacturing criteria to ensure batch-to-batch reproducibility
Most of the Cytokine/Chemokine panels provide you the flexibility to:
- Select a premixed kit using the appropriate catalogue number.
- Choose analytes within a catalogue number, or panel to design a customized kit.
How to design a customized kit:
1. Select your panel of interest: for example, HCYTOMAG-60K.
2. Choose only the analytes you want from that panel: for example, you may need only 5 analytes
IL-2, IL-6, IL-10, GM-CSF, VEGF.
3. Add the number of analytes you chose to the catalogue number: HCYTOMAG-60K-05 and list the
specific analytes.
Custom Assay Development

Need to develop a specific, sensitive, analytically validated
assay for your laboratory? We develop reliable, custom
multiplexed assays (using Luminex xMAP technology), as
well as single detection (ELISAs, GyroMark HT and RIAs)
assays for protein research, providing you with:
Reagents (immunogen design and antibody development)
Assay development
Manufacturing (commercial kits for research use only)
Contact your Protein Specialist or e-mail us at:
customassay@merckmillipore.com today!
Biomarker Wish List

Is there an analyte, panel or species you would like to see in
our portfolio?
Make your wish at: biomarkerwish@merckmillipore.com
BO NE / C A NCER / CVD
Cardiovascular
Human Bone
Human Circulating Cancer

Biomarker Panel 3
(Cat. No. HBNMAG-51K)
(Cat. No. HCCBP3MAG-58K)
(Cat. No. HCVD1MAG-67K)
Osteocalcin (OC)
BNP
FABP4
DKK1
Osteopontin (OPN)
Cathepsin D
NT proBNP
LIGHT
FGF-23
Osteoprotegerin
(OPG)
Ferritin
CK-MB
Oncostatin (OSM)
Fibroblast activation protein (FAP)
LIX/CXCL6/GCP-2
Galectin 3
CXCL16
Placental Growth
Factor (PLGF)
IGFBP3
Endocan-1 (ESM-1)
Melanoma inhibitory activity (MIA)
FABP3
IL-1
IL-6
Insulin
Leptin
PTH
SOST
TNF
Human Single Plex Bone Kits

(Cat. No. HRNKLMAG-51K-01)
Sex hormone binding globulin (SHBG)
Human Cancer / Metastasis

Biomarker Panel 1
Cancer Biomarkers
Biomarker Panel 1
AFP
IL-8/CXCL8
CA125
Leptin
CA15-3
MIF
CA19-9
Osteopontin (OPN)
CEA
Prolactin
CYFRA21-1
PSA (free)
sFas
PSA (total)
sFasL
SCF
FGF-2/FGF-basic
TGF
HCG
TNF
HE4
TRAIL
HGF
VEGF-A
IL-6

Biomarker Panel 2
(Cat. No. HCMBMAG-22K)
ADAMTS13
GDF-15
D-dimer
sICAM-1
GDF15
TRAP
Lipocalin-2/NGAL
NSE
TWEAK
Osteonectin
(SPARC)
YKL40
Osteoprotegerin
(OPG)
Human Angiogenesis / Growth

Factor
(Cat. No. HAGP1MAG-12K)
Angiopoietin-2
HB-EGF
BMP-9
HGF
EGF
IL-8/CXCL8
Endoglin
Leptin
Endothelin-1
Placental Growth
Factor (PLGF)
FGF-1/FGF-acidic
FGF-2/FGF-basic
Follistatin
G-CSF
Myeloperoxidase
(MPO)
Myoglobin
Serum Amyloid A
sP-Selectin
sVCAM-1
Human CVD Panel 3 (Acute

Phase)
-2-Macroglobulin Haptoglobin
Adipsin
sL-Selectin
AGP
Platelet Factor 4
(PF4)
CRP
Fetuin A
Fibrinogen
Serum Amyloid P
von Willebrand
Factor (vWF)
Human CVD Panel 4

VEGF-A
sE-Selectin
Pentraxin-3 (PTX3)
VEGF-C
Follistatin (FST)
Tissue Factor (TF)
VEGF-D
dPAPP-A
Thrombomodulin
sPECAM-1
Troponin T (TnT)
Human Apolipoproteins
(Cat. No. APOMAG-62K)
Complement factor H (CFH)

Extracellular matrix protein 1 (ECM1)
Download our featured white papers
and wall charts!
MILLIPLEX map Human Angiogenesis /
Growth Factor
Lit. No. AN4466ENEU
MILLIPLEX map Human CVD Biomarkers
Lit. No. TN5533ENEU
MILLIPLEX map Cancer Signaling
Wall Chart
Lit. No. PS4992ENEU
Reference the "Lit. No." when searching
online at: www.merckmillipore.com
Periostin
Antithrombin III
Vitronectin
Human CVD Panel 2
DKK1
Vitamin D binding protein
Troponin I (TnI)
Myeloperoxidase (MPO)
NEW!
RANKL
Human CVD Panel 1
ACTH
HUMAN
Bone Metabolism
Apo AI
Apo CII
Apo AII
Apo CIII
Apo B
Apo E
Human Cytokine / Chemokine

Panel I

Panel III
(Cat. No. HCYTOMAG-60K)

(Cat. No. HCYTMAG-60K-PX29)
(Cat. No. HCYP3MAG-63K)
Human Th17
(Cat. No. HTH17MAG-14K)
(Cat. No. HT17MG-14K-PX25)
29
HCC-1/CCL14
M-CSF
GM-CSF
IL-17E/IL-25
30
IL-11
MIG/CXCL9
IFN
IL-17F
38
IL-29/IFN1
MIP-3/CCL20
IL-1
IL-21
41
I-TAC/CXCL11
MIP-3/CCL19
IL-2
IL-22
NAP-2/CXCL7
IL-4
IL-23
IL-5
IL-27
IL-6
IL-28A
IL-9
IL-31
IL-10
IL-33
13
IL-12 (p70)
MIP-3/CCL20
21
IL-13
TNF
TNF/LTA
sCD40L
IL-9
LIX/CXCL6/GCP-2
EGF
IL-10
Lymphotactin/XCL1
Eotaxin/CCL11
IL-12 (p40)
FGF-2/FGF-basic
IL-12 (p70)
Human High Sensitivity T Cell
Flt3 ligand
IL-13
(Cat. No. HSTCMAG-28SK)

(Cat. No. HSTCMAG28SPMX13)
(Cat. No. HSTCMAG28SPMX21)
Fractalkine /CX3CL1 IL-15

G-CSF
IL-17A
GM-CSF
IP-10/CXCL10
Fractalkine/CX3CL1 IL-12 (p70)
IL-15
GRO
MCP-1/CCL2
GM-CSF
IL-13
IL-17A
IFN2
MCP-3/CCL7
IFN
IL-17A
IFN
MDC/CCL22
IL-1
IL-21
Human CD8+ T Cell
IL-1
MIP-1/CCL3
IL-2
IL-23
IL-1
MIP-1/CCL4
IL-4
ITAC
(Cat. No. HCD8MAG-15K)

(Cat. No. HCD8MAG15K17PMX)
IL-1ra
PDGF-AA
IL-5
MIP-1/CCL3
sCD137
IL-5
IL-2
PDGF-AB/BB
IL-6
MIP-1/CCL4
sFas
IL-6
IL-3
RANTES/CCL5
IL-7
MIP-3/CCL20
sFasL
IL-10
IL-4
TGF
IL-8/CXCL8
TNF
GM-CSF
IL-13
IL-5
TNF
IL-10
Granzyme A
MIP-1/CCL3
IL-6
TNF/LTA
Granzyme B
MIP-1/CCL4
IL-7
VEGF-A
IFN
Perforin
IL-2
TNF
Human Soluble Cytokine

Receptor NEW!
IL-8/CXCL8

Panel II
(Cat. No. HCYP2MAG-62K)
(Cat. No. HCP2MAG-62K-PX23)
6Ckine/CCL21
I-309/CCL1
BCA-1/CXCL13
LIF
CTACK/CCL27
ENA-78/CXCL5
23
(Cat. No. HSCRMAG-32K)

(Cat. No. HSCRMAG32KPX14)
sIL-6R
Human MMP Panel 1
sRAGE
(Cat. No. HMMP1MAG-55K)
sgp130
sTNF RI
MCP-2/CCL8
sIL-1RII
sVEGF R1
MCP-4/CCL13
sIL-2R
sVEGF R2
Eotaxin-2/CCL24/
MPIF-2
MIP-1/MIP-5/
CCL15
sIL-4R
sVEGF R3
Eotaxin-3/CCL26
SCF
IL-16
SDF-1/CXCL12
IL-20
TARC/CCL17
IL-21
TPO
IL-23
TRAIL/TNFSF10
IL-28A
TSLP
MMP-3
MMP-13
MMP-12
Human MMP Panel 2

(Cat. No. HMMP2MAG-55K)
MMP-1
MMP-9
MMP-2
MMP-10
MMP-7
Human TIMP Panel 1

Serum/Plasma samples
(Cat. No. HTMP1MAG-54K)
Cannot be plexed together.
1. PSA (free) and PSA (total)
Available in cat. no. listed
These analytes cannot be plexed with
other analytes in this panel in serum/
plasma
TIMP-1
TIMP-2
IL-33/NF-HEV
(mature)
17
14
sEGFR
sTNF RII
25
IL-4
sCD30
sIL-1RI
Immunology
Immunology/Immune Response
% Requires a protease inhibitor during

sample collection
I M M U NO LO GY / M ETA BO LISM
Immunology/Immune
Response, continued
Human Liver Protein
Human TIMP Panel 2
Human Skin
Cell culture samples

(Cat. No. HTMP2MAG-54K)
(Cat. No. SKINMAG-50K)
AFP
FGF-19
TIMP-3
Fibronectin
Keratin-1,10
ANGPTL3
FGF-21
TIMP-2
TIMP-4
Human Serum
Albumin (HSA)
Keratin-6
ANGPTL4
FGF-23
LPS
ANGPTL6
HGF
Involucrin
Human Immunoglobulin
Isotyping
FABP1
Metabolism/
Endocrinology
(Cat. No. HGAMMAG-301K)

IgA
IgG3
IgG1
IgG4
IgG2
IgM
Human Single Plex Immunoglobulin Isotyping Kits

(Cat. No. HGAMMAG-303E)
IgE
(Cat. No. HIGFBMAG-53K)

IGFBP1
IGFBP5
IGFBP2
IGFBP6
(Cat. No. HADK1MAG-61K)
IGFBP3
IGFBP7
Adiponectin
PAI-1 (total)
Adipsin
Resistin
IGFBP4
Human IGF
(Cat. No. HIGFMAG-52K)
IGF-1
IGF-2
Human Adipokine Panel 2
(Cat. No. HSP1MAG-63K)
(Cat. No. HADK2MAG-61K)
Human Pituitary Panel 1
sFas
MIF
sFasL
PAI-1 (total)
HGF
Leptin
ACTH
GH
sICAM-1
sVCAM-1
IL-1
MCP-1/CCL2
AGRP
LH
IL-6
NGF
CNTF
TSH
IL-8/CXCL8
TNF
FSH
Human Sepsis Panel 2

MIP-1/CCL3
HSP70
MIP-1/CCL4
Human Adipocyte
IL-1
MMP-8

(Cat. No. HADCYMAG-61K)
Adiponectin
MCP-1/CCL2
HGF
NGF
IL-1
PAI-1 (total)
Lactotransferrin (LTF)
IL-6
Resistin
Lipocalin-2/NGAL
IL-8/CXCL8
TNF
Neutrophil Elastase-2 (ELA2)
Leptin

Human Metabolic Hormone
Thrombospondin-1 (TSP-1)
(Cat. No. HMHEMAG-34K)

FGF-13
MMP-8
NEW!
Olfactomedin-4
(OLFM-4)

Elastase-2
Proteinase-3
(PRTN-3)
Amylin (active)
IL-6
Amylin (total)
Insulin
C-Peptide
Leptin
Ghrelin (active) %
MCP-1/CCL2
GIP (total)
Pancreatic
Polypeptide
GLP-1 (active)
NEW!
GLP-1 (total)
Glucagon
Human Pituitary Panel 2

(Cat. No. HPTP2MAG-66K)
BDNF
Prolactin
** To be discontinued
Resistin
(Cat. No. HPTP1MAG-66K)
Insulin
Granzyme B
IL-8/CXCL8
Human IGF Binding Protein
Human Adipokine Panel 1
Lipocalin-2/NGAL
(Cat. No. HLPPMAG-57K)
TIMP-1
Cortisol
PYY (total)
TNF
Cannot be plexed together:

1. Amylin (active) and Amylin (total)
2. GLP-1 (active) and GLP-1 (total)
sample collection
n
Requires Sample Extraction
Download our featured wall chart!

MILLIPLEX map Neuroendocrine
Mechanisms in Appetite & Obesity
Lit. No. PS2550ENEU
Human Neurodegenerative
Disease Panel 1
Human Neurological Disorders

Panel 2
(Cat. No. HNDG1MAG-36K)
(Cat. No. HND2MAG-39K)
-2-Macroglobulin Complement C3
Apo AI
Apo CIII
Apo E
Complement Factor
H (CFH)
-1-Acid
Glycoprotein (AGP)
Ceruloplasmin (CP)

MIP-4
Contactin-1
Complement C4
PEDF
Fetuin A
CRP
SAP
Kallikrein-6
NEW!
KIM-1
Serum Amyloid P
FABP1
Osteopontin (OPN)
GST
PTH
IP-10
Renin
-1-Antitrypsin
EGF

Panel 3
Angiotensinogen
(AGT)
(Cat. No. HKI4MAG-99K)
Osteopontin (OPN)
Soluble Superoxide
Dismutase 1
(sSOD1)
Soluble Superoxide
Dismutase 2
(sSOD2)
Human Kidney Injury Panel 5

NEW!
-1-Microglobulin
Osteoactivin
Collagen IV
TIMP-1
GST
Uromodulin
Lipocalin-2/NGAL
Disease Panel 3
Human Neuropeptide
(Cat. No. HNPMAG-35K) n
BDNF
PAI-1 (total)
-MSH
Neurotensin
Cathepsin D
PDGF-AA
-Endorphin
Orexin A
-2-Microglobulin
Cystatin C
sICAM-1
PDGF-AB/BB
Cortisol
Oxytocin
Clusterin
RBP4
MPO
RANTES
Melatonin
Substance P
sNCAM
sVCAM-1
Serum samples
(Cat. No. HKTX1MAG-38K) **
Toxicity
CSF samples
sRAGE
A1-42
S100B
GDNF

Panel 1
CSF samples
Urine samples
NEW!
Serum samples
Osteoactivin
FABP1
Renin
-2-Microglobulin
GST
TFF-3
Clusterin
GST
TIMP-1
Cystatin C
Human Kidney Toxicity Panel 3
IP-10/CXCL10
NGF
Tau (Thr231)
Neuron-Specific
Enolase (NSE)
Tau (total)
Urine samples
Urine samples
KIM-1
NEW!
TFF-3
Renin
-1-Microglobulin
EGF
Albumin
Lipocalin-2/NGAL
Clusterin
Osteopontin (OPN)
Urine samples
Cystatin C

Urine samples
-2-Microglobulin
Albumin
Cystatin C
-2-Microglobulin
Osteopontin (OPN)
Clusterin

Collagen IV
MILLIPLEX map Inflammation in

Neurodegenerative Disease
Lit. No. PS5724ENEU
Renin
Osteopontin (OPN)
KIM-1
PARK7/DJ1
Download our featured wall chart!
KIM-1
Calbindin
-Synuclein
PARK5/UCHL1
NEW!
Disease Panel 4
A1-40
Human
Disease Panel 2
Haptoglobin (HP)
Prealbumin
NEU RO SCIENCE / TOX I CI T Y
Neuroscience
NEW!
Uromodulin
RBP4
IMMU NO LO GY / M ETA BO LI SM / BO NE / C A NCER
Non-Human Primate
Cytokine/Chemokine
(Cat. No. PRCYTOMAG-40K)
(Cat. No. PCYTMG-40K-PX)
sCD40L
IL-12/23 (p40)
G-CSF
IL-13
GM-CSF
IL-15
IFN
IL-17A
IL-1
IL-18
IL-1ra
MCP-1/CCL2
IL-2
MIP-1/CCL3
IL-4
MIP-1/CCL4
IL-5
TGF
IL-6
TNF
IL-8/CXCL8
VEGF-A
23
sCD137
IL-117E/IL-25
Eotaxin/CCL11
IL-21
sFasL
IL-22
FGF-2/FGF-basic
IL-23
Bone Metabolism
Non-Human Primate
Metabolic Hormone
(Cat. No. MBNMAG-41K)
(Cat. No. NHPMHMAG-45K)
ACTH
Osteocalcin (OC)
DKK-1
Osteoprotegerin
(OPG)
Amylin (active)
Insulin
C-Peptide
Leptin
IL-6
Ghrelin (active) %
MCP-1/CCL2
Insulin
GIP (total)
Pancreatic
Polypeptide
Leptin
IL-6
SOST
TNF
PYY (total)
Mouse Single Plex Bone Kits
TNF
(Cat. No. MRNKLMAG-31K-01)

RANKL
Non-Human Primate Pituitary

Panel 1
(Cat. No. NHPPT1MG-46K)
24
25
ACTH
GH
AGRP
LH
CNTF
TSH
FSH
Non-Human Primate Pituitary

Panel 2
(Cat. No. NHPPT2MG-46K)
BDNF
Prolactin
Cancer Biomarkers
Mouse Angiogenesis / Growth
Factor Panel 1
(Cat. No. MAGPMAG-24K)
sALK-1
IL-6
Amphiregulin
IL-17A
Angiopoietin-2
KC/CXCL1
Betacellulin
Leptin
sCD31/sPECAM-1 MCP-1/CCL2
EGF
MIP-1/CCL3
Endoglin
PLGF-2
Fractalkine/CX3CL1 IL-28A
Endothelin-1
Prolactin
Granzyme A
IL-31
sFASL
SDF-1
Granzyme B
IL-33
FGF-2
TNF
IL-1
IP-10/CXCL10
Follistatin
VEGF-A
IL-2
MIP-3/CCL20
G-CSF
VEGF-C
IL-4
Perforin
HGF
VEGF-D
IL-6
RANTES/CCL5
IL-1
IL-16
TNF/LTA
IL-17A
Available in cat. no. listed

These analytes cannot be plexed with
other analytes in this panel in serum/
plasma
sample collection
Mouse Bone
FGF-23
Glucagon
Non-Human PrimateCytokine/
NEW!
Chemokine Panel 2
(Cat. No. PRCYT2MAG40K)
(Cat. No. PRCYTMAG40K-PX24)
(Cat. No. PRCYTMAG40K-PX25)
Metabolism/
Endocrinology
GLP-1 (active)
IL-10
Primate
Immunology
Immunology/Immune Response
Mouse CVD1
Mouse Cytokine / Chemokine

Panel I
Mouse Soluble Cytokine

Receptor
(Cat. No. MCYTOMAG-70K)

(Cat. No. MCYTOMAG-70K-PMX) 25
32
(Cat. No. MCYTMAG-70K-PX32)
(Cat. No. MSCRMAG-42K)
(Cat. No. MCVD1MAG-77K)

sCD31/sPECAM-1
PAI-1 (total)
sE-Selectin
sP-Selectin
sICAM
Thombomodulin
MMP-9
Mouse CVD2
(Cat. No. MCVD2MAG-77K)
NEW!
sCD30
sRAGE
sgp130
sTNF RI
Eotaxin/CCL11
IL-13
sIL-1RI
sTNF RII
G-CSF
IL-15
sIL-1RII
sVEGF R1
GM-CSF
IL-17A
sIL-2R
sVEGF R2
IFN
IP-10/CXCL10
sIL-4R
sVEGF R3
sIL-6R
Oncostatin-M
IL-1
KC/CXCL1
CXCL16
Placental Growth
Factor (PLGF-2)
IL-1
LIF
IL-2
LIX/CXCL6/GCP-2
Troponin I
Mouse Th17
IL-3
MCP-1/CCL2
Troponin T
IL-4
M-CSF
(Cat. No. MTH17MAG-47K)

(Cat. No. MT17MAG47K-PX25)
IL-5
MIG/CXCL9
sCD40L
IL-17E/IL-25
IL-6
MIP-1/CCL3
GM-CSF
IL-17F
Mouse Acute Phase Panel 1
IL-7
MIP-1/CCL4
IFN
IL-21
(Cat. No. MAP1MAG-76K)
IL-9
MIP-2/CXCL2
IL-1
IL-22
IL-10
RANTES/CCL5
IL-2
IL-23
IL-12 (p40)
TNF
IL-4
IL-27
IL-12 (p70)
VEGF-A
IL-5
IL-28B
IL-6
IL-31
IL-10
IL-33
IL-12 (p70)
MIP-3/CCL20
IL-13
TNF
TNF/LTA
Endocan-1
FABP4
Follistatin
LIGHT
Lipocalin-2/NGAL
Pentraxin-3 (PTX3)
Serum Amyloid A-3

(SAA-3)
Mouse Acute Phase Panel 2

(Cat. No. MAP2MAG-76K)
Adipsin
CRP
-1-Acid
glycoprotein (AGP)
Haptoglobin
-2-Macroglobulin
Serum Amyloid P

Panel II
(Cat. No. MCYP2MAG-73K)
IL-21
MCP-5/CCL12
IL-15
IL-22
MIP-3/CCL20
IL-17A
IL-17E/IL-25
MIP-3/CCL19
IL-28B
TARC/CCL17
25
MOUSE
sCD40L
CVD / IM M U NO LO GY
Cardiovascular

Panel III
(Cat. No. MCYP3MAG-74K)
IL-20
IL-33
IL-23
MDC/CCL22
IL-27
TIMP-1
IMMUNOLOGY / META BO L I SM / NEU RO SCI ENCE / Toxicit y / bone
Metabolism/
Endocrinology
Mouse CD8+ T Cell
Mouse Adipokine
(Cat. No. MCD8MAG-48K)

(Cat. No. MCD8MAG48K-PX15)
15
(Cat. No. MADKMAG-71K)
sCD137
IL-5
IL-6
PAI-1 (total)
sFas
IL-6
Insulin
Resistin
sFasL
IL-10
Leptin
TNF
GM-CSF
IL-13
MCP-1/CCL2
Granzyme B
MIP-1/CCL4
IFN
RANTES/CCL5
Mouse Adipocyte
IL-2
TNF

(Cat. No. MADCYMAG-72K)
IL-4
Rat/Mouse Neuropeptide
(Cat. No. RMNPMAG-83K) n
-MSH
Orexin A
-Endorphin
Oxytocin
Neurotensin
Substance P
Toxicity
Mouse Kidney Injury Panel 1
Urine samples
-2-Microglobulin
Renin
PAI-1 (total)
Mouse MMP Panel 1
IP-10/CXCL10
TIMP-1
IL-6
Resistin
(Cat. No. MMMP1MAG-79K)
KIM-1
VEGF-A
Leptin
TNF
MMP-8
MMP-3
Mouse MMP Panel 2

proMMP-9
MMP-12
Mouse MMP Panel 3

Mouse Adiponectin Single

Plex
(Cat. No. MADPNMAG-70K-01)
Adiponectin
Mouse Metabolic Hormone

(Cat. No. MMHMAG-44K)
(Cat. No. RBN1MAG-31K)
Glucagon
IgA
IgG2b
IgG1
IgG3
IgG2a
IgM
Mouse Single Plex

Immunoglobulin Isotyping
Kits
(Cat. No. MGAMMAG-300E)
IgE

sample collection
n
Requires Sample Extraction
Fibrinogen cannot be plexed with

other analytes in this panel in serum
Bone Metabolism
Pancreatic
Polypeptide
GLP-1 (active)
(Cat. No. MGAMMAG-300K)
Osteopontin (OPN)
EGF
Rat Bone Panel 1
GIP (total)
IL-6
PYY (total)
Resistin
TNF
ACTH
Osteocalcin (OC)
DKK1
Osteoprotegerin
(OPG)
FGF-23
Insulin
Leptin
Insulin
NEW!
Lipocalin-2/NGAL
MCP-1/CCL2
MMP-12
Cystatin C
Leptin
MMP-3
NEW!
Clusterin
C-Peptide 2
Ghrelin (active) %
Mouse Immunoglobulin
Isotyping
Urine samples
Amylin (active)
proMMP-9
Mouse Kidney Injury Panel 2
MCP-1/CCL2
MMP-2
MMP-8
10
Neuroscience
Adiponectin
MMP-2
mouse
Immunology/Immune
Response, continued
PTH
SOST
Mouse IGF Binding Protein
Rat Bone Panel 2
(Cat. No. MIGFBPMAG-43K)

(Cat. No. RBN2MAG-31K)
IGFBP1
IGFBP5
IGFBP2
IGFBP6
ACTH
Osteopontin (OPN)
IGFBP3
IGFBP7
DKK-1
Osteoprotegerin
(OPG)
FGF-23
Mouse Pituitary
Insulin
(Cat. No. MPTMAG-49K)
Leptin
ACTH
LH
BDNF
Prolactin
FSH
TSH
GH3
PTH
SOST
Osteocalcin (OC)
Rat Single Plex Bone Kits

(Cat. No. RBNMAG-31K-1OC)
(Cat. No. RBNMAG-31K-1OPN)
(Cat. No. RRNKLMAG-31K-01)
Osteocalcin (OC)
Osteopontin (OPN)
RANKL
Metabolism/
Endocrinology
Neuroscience
Rat Cytokine / Chemokine
Rat Adipokine
(Cat. No. RMNPMAG-83K) n
(Cat. No. RECYTMAG-65K)

(Cat. No. RECYMAG65K27PMX)
(Cat. No. RADPKMAG-80K)
27
Rat/Mouse Neuropeptide
EGF
IL-12 (p70)
IL-1
MCP-1/CCL2
Eotaxin/CCL11
IL-13
IL-6
PAI-1 (active)
Fractalkine /CX3CL1 IL-17A
Insulin
PAI-1 (total)
G-CSF
IL-18
Leptin
TNF
GM-CSF
IP-10/CXCL10
GRO/KC/CINC-1
Leptin
Rat Adipocyte
IFN
LIX/CXCL6/GCP-2
IL-1
MCP-1/CCL2

(Cat. No. RADPCMAG-82K)
IL-1
MIP-1/CCL3
Adiponectin
PAI-1 (active)
IL-2
MIP-2/CCL8
IL-1
PAI-1 (total)
IL-4
RANTES/CCL5
IL-6
TNF
IL-5
TNF
Leptin
IL-6
VEGF-A
NEW!
Rat Immunoglobulin Isotyping

(Cat. No. RGAMMAG-302K)
(Cat. No. RADPNMAG-81K-01)
Adiponectin
IgG2c
Rat Metabolic Hormone
IgG2a
IgM
(Cat. No. RMHMAG-84K)

Amylin (active)
Insulin
C-Peptide 2
Leptin
Ghrelin (active) %
MCP-1/CCL2
GIP (total)
Pancreatic
Polypeptide
GLP-1 (active)
Glucagon
IL-6
PYY (total)
TNF
ACTH
LH
BDNF
Prolactin
FSH
TSH
GH
NEW!
(Cat. No. RSHMAG-69K)

Melatonin
Corticosterone
Rat Thyroid
Calbindin
KIM-1
Clusterin
Osteopontin (OPN)
GST
TIMP-1
IP-10/CXCL10
VEGF-A
NEW!
Urine samples
(Cat. No. RKTX2MAG-37K)
AGP
Cystatin C
Albumin
EGF
-2-Microglobulin
Lipocalin-2/NGAL
Rat Vascular Injury Panel 1

(Cat. No. RV1MAG-26K)
NEW!
Caveolin-1
PAI-1 (total)
CINC-1/GRO/KC
TIMP-1
CTGF (Connective
Tissue Growth
Factor)
TNF
VEGF-A

Adiponectin
TSH
NEW!
von Willebrand
Factor (vWF)
sICAM-1

-1-Acid
Glycoprotein (AGP)
-2-Macroglobulin
(A2M)
NEW!
Fibrinogen
Haptoglobin
(Cat. No. RTHYMAG-30K)

T3
Urine samples
(Cat. No. RKTX1MAG-37K)
sE-Selectin
Rat Stress Hormone
MILLIPLEX map Rat Kidney Tox

Lit. No. AN6622ENEU
Substance P
MCP-1
(Cat. No. RPTMAG-86K)
Download our featured white paper!
Neurotensin
IL-6
Rat Pituitary
ACTH
Oxytocin
rat
IgG1
IgE
-Endorphin
Rat Kidney Toxicity Panel 2
IgG2b
(Cat. No. RGAMMAG-302E)
Orexin A
Rat Kidney Toxicity Panel 1
IgA
Rat IgE Single Plex
-MSH
Toxicity
Rat Adiponectin
IL-10
IM M U NO LO GY / M ETA BO LI SM / NEU RO SC I ENCE / TOXICIT Y
Immunology/Immune
Response
T4

11
I MMUNO LO GY / M ETA BO LISM / TOXCIT Y
Immunology
Immunology
Immunology
Canine Cytokine / Chemokine
Feline Cytokine / Chemokine
Porcine Cytokine / Chemokine
(Cat. No. CCYTOMAG-90K)

(Cat. No. CCYTMG-90K-PX13)
(Cat. No. FCYTOMAG-20K)

(Cat. No. FCYTMAG-20K-PMX)
13
(Cat. No. PCYTMAG-23K)

(Cat. No. PCYTMG-23K-13PX)
GM-CSF
IL-15
sFas
IL-13
GM-CSF
IL-6
IFN
IL-18
FLt3 ligand
IL-18
IFN
IL-8/CXCL8
IL-2
IP-10/CXCL10
GM-CSF
KC
IL-1
IL-10
IL-6
KC-like
IFN
MCP-1/CCL2
IL-1ra
IL-12
IL-7
MCP-1/CCL2
IL-1
PDGF-BB
IL-1
IL-18
IL-8/CXCL8
TNF
IL-2
RANTES
IL-2
TNF
IL-4
SCF
IL-4
IL-6
SDF-1
IL-8/CXCL8
TNF
IL-10
Metabolism/
Endocrinology
Amylin (total)
Insulin
Ghrelin (active) %
Leptin
Feline Metabolic Hormone
GIP (total)
Pancreatic
Polypeptide
(Cat. No. FMHMAG-20K)
GLP-1 (active)
Glucagon
PYY (total)
Leptin
GIP (total)
Pancreatic
Polypeptide (PP)
Glucagon
Prolactin
FSH
TSH
NEW!
Ghrelin (active) %
(Cat. No. CPTMAG-96K)

BDNF
TGF1
Insulin
GLP-1 (active)
LH
(Cat. No. TGFBMAG-64K-01)
Amylin (active)
Canine Pituitary
ACTH
Multi-species TGF - Single

Plex
Metabolism/
Endocrinology
(Cat. No. CGTMAG-98K)
PYY (total)
Multi-species TGF - 3 Plex

(Cat. No. TGFBMAG-64K-03)
TGF1
TGF3
TGF2
Metabolism/
Endocrinology
Multi-species Steroid/Thyroid
Hormone
GH
(Cat. No. STTHMAG-21K)
Toxicity
Cortisol
T3
Estradiol
T4
Progesterone
Canine Kidney Toxicity

Expanded Panel 1
Urine samples
(Cat. No. CKT1MAG-97K)
NEW!
Clusterin
Lipocalin-2/NGAL
Cystatin C
MCP-1
KIM-1
Osteopontin (OPN)
IL-8/CXCL8

sample collection

12
13
Immunology
IL-12 (p40)
Canine Gut Hormone
other
19
Panel only premix
Intracellular Multiplex
Assays
Phosphoprotein and Total Detection
New Challenges, Faster Answers
Your research can take you from studying circulating to
intracellular biomarkers or even both at the same time.
This is where our MILLIPLEX map intracellular multiplex
panels and MAPmate single plex assays help.
Multiplexing, using the Luminex xMAP platform,
provides faster answers to your intracellular questions
compared to traditional Western blots, mass spectrometry
and radioactive phosphorylation assays that require large
amounts of sample.
We proudly offer the widest range of analytes and
techniques in our MILLIPLEX map intracellular portfolios.
Cellular Metabolism Multiplex

Assays
Compare protein levels with results from activity
assays
Acquire a deeper understanding of intracellular
pathways regulating energy production, mitochondrial
dysfunction and toxicity, metabolic and neurological
disorders, cancer and aging

Directly compare the total vs. phosphoprotein levels
in your assay by reading them in the same well
Conduct these measurements using minimal sample
volumes
Cell Signaling Phosphoprotein &

Total Multiplex Assays

Measure multiple total or phosphoproteins in a single
sample
Conduct these measurements using minimal sample
volumes
MAPmate Phosphoprotein &

Total Single Plex Kits
INTRACELLULAR
Q
uickly measure levels of multiple proteins within
panels using small samples
Cell Signaling Phosphoprotein +

Total 2 Plex Assays

Plex up to 8 individual MAPmate kits to design your
own custom assays using our Cell Signaling Buffer
and Detection Kit*
Plex additional MAPmate kits into existing
MILLIPLEX map Cell Signaling kits*
Use tubulin or GAPDH as housekeeping/loading
controls by plexing into an existing MILLIPLEX map
Cell Signaling kit*
*Refer to the guidelines provided in the protocols. Buffer
compatibility information is available in this publication.
13
C EL LU L A R M ETA BO LI SM / CELL SIG NA LING
Cellular Metabolism Assays

Human Glycolysis Pathway
Cell Signaling Phosphoprotein +

Total 2 Plex Assays
(Cat. No. HGPMAG-27K)
Akt Phospho/Total 2 Plex
ENO1
(Cat. No. 48-618MAG) AB2
G6PI
Analyte
HIF-1
Akt
LDHA
Akt
Total
Phosphorylated
3 (Ser473)
LDHB
PKM2
Erk/MAPK 1/2 Phospho/Total 2 Plex
TKT
Human Oxidative Phosphorylation

(Cat. No. H0XPSMAG-16K)
Analyte
Total
Erk/MAPK 1/2
Erk/MAPK 1/2
Phosphorylated
3 (Thr185/Tyr187)
Complex I (NADH-Ubiquinone Oxidoreductase)

Complex II (Succinate Ubiquinone Oxidoreductase)
JNK Phospho/Total 2 Plex
Complex III (Ubiquinone Cytochrome C Oxidoreductase)
Complex IV (Cytochrome C Oxidase)
Analyte
Complex V (ATP Synthase)
JNK
NNT (Nicotinamide Nucleotide Transhydrogenase)
JNK
3 (Thr183/Tyr185)
3
STAT3 Phospho/Total 2 Plex
(Cat. No. RM0XPSMAG-17K)
Complex I (NADH-Ubiquinone Oxidoreductase)
Analyte
Complex III (Ubiquinone Cytochrome C Oxidoreductase)
STAT3
Complex V (ATP Synthase)
STAT3
(Cat. No. H0XSTMAG-18K)
Catalase
INTRACELLULAR
Phosphorylated
Rat/Mouse Oxidative Phosphorylation
Human Oxidative Stress
PRX2 (PRDX2)
SOD1
SOD2
TRX1
Multi-species Pyruvate Dehydrogenase

(PDH) Complex
(Cat. No. PDHMAG-13K)
Analyte
Total
PDH
Phosphorylated
3
PDH
3 (Ser232)
PDH
3 (Ser293)
PDH
3 (Ser300)
14
Total
NEW!
Total
Phosphorylated
3 (Tyr705)
Akt/mTOR (Phosphoprotein) 11 Plex
Early Apoptosis 7 Plex
Analyte
Total
Phosphorylated
Analyte
Akt
3 (Ser473)
Akt
3 (Ser473)
GSK3a
3 (Ser21)
BAD
3 (Ser112)
GSK3b
3 (Ser9)
Bcl-2
IGF1R
3 (Tyr1135/1136)
Active Caspase 8
IR
3 (Tyr1162/1163)
Active Caspase 9
IRS1
3 (Ser636)
JNK
3 (Thr183/Tyr185)
mTOR
3 (Ser2448)
p53
3 (Ser46)
p70S6 Kinase
3 (Thr389/412)
PTEN
3 (Ser380)
RPS6
3 (Ser235/236)
TSC2
3 (Ser939)
Total
Human Late Apoptosis 3 Plex

Analyte
Total
3
Akt/mTOR (Total) 11 Plex
Cleaved PARP
GAPDH
Analyte
Total
Akt
GSK3a
GSK3b
IGF1R
IR
IRS1
mTOR
p70S6 Kinase
PTEN
RPS6
TSC2
Phosphorylated
Phosphorylated
Phosphorylated
3 (Ser70)
Active Caspase 3
Cell Signaling
Cell Signaling
Premix panel only
AB1: Uses Assay Buffer 1

15
CELL SIG NA LING
Cell Signaling, continued

Human DNA Damage/Genotoxicity 7 Plex
Analyte
ATR
Total
Phosphorylated
MAPK/SAPK (Phosphoprotein) 10 Plex

Analyte
Total
Phosphorylated
ATF2
3 (Thr71)
Chk1
3 (Ser345)
ErK/MAPK 1/2
3 (Thr185/Tyr187)
Chk2
3 (Thr68)
HSP27
3 (Ser78)
H2A.X
3 (Ser139)
JNK
3 (Thr183/Tyr185)
c-Jun
3 (Ser73)
MEK1
3 (Ser222)
MSK1
3 (Ser212)
p38 (SAPK2A/B)
3 (Thr180/Tyr182)
Human EGFR Profiler 8 Plex
p53
3 (Ser15)
STAT1
3 (Tyr701)
MDM2
p21
p53
Analyte
EGFR
3 (Ser15)
Total
Phosphorylated
EGFR
3 (Thr654)
EGFR
3 (Thr669)
EGFR
3 (Tyr845)
EGFR
3 (Tyr1045)
EGFR
3 (Ser1047)
EGFR
3 (Tyr1068)
EGFR
3 (Tyr1086)
Human Mitogenesis RTK

(Phosphoprotein) 7 Plex
Analyte
Total
Phosphorylated
cMet/HGFR
3 (pan Tyr)
EGFR
3 (pan Tyr)
ErbB2/HER2
3 (pan Tyr)
ErbB3
3 (pan Tyr)
ErbB4
3 (pan Tyr)
Human Heat Shock Protein 5 Plex
IGF1R
3 (pan Tyr)
IR
3 (pan Tyr)
Analyte
HSP27
3 (Ser78/Ser82)
3
HSP70
HSP90
Phosphorylated
HSP27
HSP60
Total
Human Mitogenesis RTK (Total) 7 Plex

Analyte
Total
cMet/HGFR
EGFR
ErbB2/HER2
ErbB3
ErbB4
IGF1R
IR
Phosphorylated
Download our featured "white paper!

MILLIPLEX map DNA Damage/Genotoxicity
Lit. No. TN3300ENEU
16
Multi-Pathway (Phosphoprotein) 9 Plex
STAT (Phosphoprotein) 5 Plex
Phosphorylated
Analyte
Akt
3 (Ser473)
STAT1
3 (Tyr701)
CREB
3 (Ser133)
STAT2
3 (Tyr690)
Erk/MAPK 1/2
3 (Thr185/Tyr187)
STAT3
3 (Tyr705)
NFkB
3 (Ser536)
STAT5A/B
3 (Tyr694/699)
JNK
3 (Thr183/Tyr185)
STAT6
3 (Tyr641)
p38
3 (Thr180/Tyr182)
p70S6 Kinase
3 (Thr389/412)
STAT3
3 (Ser727)
STAT5A/B
3 (Tyr694/699)
Analyte
Total
Human NFkB 6 Plex

Analyte
c-Myc
Total
Phosphorylated
Total
Phosphorylated
T-Cell Receptor (Phosphoprotein) 7 Plex

Analyte
Total
Phosphorylated
CD3e
3 (pan Tyr)
CREB
3 (Ser133)
Erk/MAPK 1/2
3 (Thr185/Tyr187)
LAT
3 (pan Tyr)
Lck
3 (pan Tyr)
FADD
3 (Ser194)
Syk
3 (pan Tyr)
IkB
3 (Ser32)
ZAP-70
3 (pan Tyr)
IKK/b
3 (Ser177/Ser181)
NFkB
3 (Ser536)
TNFRI
TGF 6 Plex
Analyte
Total
Phosphorylated
Src Family Kinase Active Site

(Phosphoprotein) 8 Plex
Akt
3 (Ser473)
Erk/MAPK 1/2
3 (Thr185/Tyr187)
SMAD2
3 (Ser465/467)
Analyte
Total
Cell Signaling
Phosphorylated
SMAD3
Blk
3 (Tyr389)
SMAD4
Fgr
3 (Tyr412)
TGFRII
Fyn
3 (Tyr420)
Hck
3 (Tyr411)
Lck
3 (Tyr394)
Lyn
3 (Tyr397)
Src
3 (Tyr419)
Yes
3 (Tyr421)
3 (Ser423/425)
Premix panel only

17
I NTRACELLU L A R M A Pma te KIT S
MAPmate Phosphoprotein & Total Single Plex Kits

Plex up to 8 individual MAPmate assays together using
our Cell Signaling Buffer and Detection Kit or include
them in existing MILLIPLEX map Cell Signaling Panels to
enhance the panel or serve as controls within the
guidelines provided in the protocols.
The following MAPmate assays should not be

plexed together:
Important MAPmate Rules

Phospho-specific and total MAPmate pairs, e.g.
total GSK3 and phospho-GSK3 (Ser9).
Consult the protocol prior to use.

All magnetic MAPmate assays require the Cell
Signaling Buffer & Detection Kit (48-602MAG).
This kit contains all necessary reagents except the
MAPmate assay. Both a filter and flat bottom plate
are included for convenience.

To select the appropriate buffer for your MAPmate
assays, please refer to the protocols, this publication
or the buffer selection tables on the website.
18

Magnetic and non-magnetic bead MAPmate assays.

MAPmate assays that require different assay
buffers.

Pan Tyr and site-specific MAPmate assays, e.g.
phospho-EGF Receptor (pan Tyr) and phospho-STAT1
(Tyr701).

More than 1 phospho-MAPmate assay for a single
target, e.g. pAkt, pSTAT3.

GAPDH and -Tubulin assays cannot be plexed with
kits or MAPmate assays containing pan Tyr assays.
Magnetic Bead MAPmate kits
Cat. No.
Species Homology
AB1
AB2
-Tubulin (Total)
46-713MAG
H,M,R
GAPDH (Total)
46-667MAG
Akt/PKB (assay buffer 1) (Ser473)
46-601MAG
H,M,R
Akt/PKB (assay buffer 1) (Thr308)
46-645MAG
H,M,R
Akt/PKB (assay buffer 1) (Total)
46-605MAG
H,M,R
Akt/PKB (assay buffer 2) (Ser473)
46-677MAG
H,M,R
Akt/PKB (assay buffer 2) (Total)
46-675MAG
H,M,R
BAD (Ser112)
46-694MAG
H,M
BAD (Total)
46-695MAG
H,M
Caspase 3 (Active)
46-604MAG
H,M
c-Jun (Ser73)
46-622MAG
H,M,R
c-Kit (pan Tyr)
46-619MAG
c-Kit (Total)
46-620MAG
c-Met/HGFR (pan Tyr)
46-651MAG
c-Met/HGFR (Total)
46-650MAG
CREB (Ser133)
46-631MAG
H,M,R
CREB (Total)
46-632MAG
H,M,R
EGF Receptor (pan Tyr)
46-603MAG
EGF Receptor (Total)
46-606MAG
ERK/MAPK 1/2 (Thr185/Tyr187)
46-602MAG
H,M,R
ERK/MAPK 1/2 (Total)
46-609MAG
H,M,R
GSK3 (Ser9)
46-690MAG
H,M,R
GSK3 (Total)
46-689MAG
H,M,R
H2A.X (Ser139)
46-692MAG
H,M
HIF-1 (Total)
46-665MAG
H,M,R
Cat. No.
Species Homology
AB1
AB2
HSP27 (Ser78)
46-607MAG
HSP27 (Total)
46-608MAG
IRS1 (pan Tyr)
46-627MAG
H,M,R
IRS1 (Total)
46-628MAG
H,M,R
IB (Ser32)
46-643MAG
IB (Total)
46-644MAG
JNK/SAPK1 (Thr183/Tyr185)
46-613MAG
H,M
JNK/SAPK1 (Total)
46-618MAG
H,M
Lck (Tyr394)
46-712MAG
H,M,R
MEK1 (Ser222)
46-670MAG
H,M,R
MEK1 (Total)
46-669MAG
H,M
mTOR (Ser2448)
46-686MAG
H,M,R
mTOR (Total)
46-685MAG
H,M,R
NFB (Ser536)
46-702MAG
NFB (Total)
46-701MAG
p21 (Total)
46-621MAG
p38/SAPK2 (Thr180/Tyr182)
46-610MAG
H,M,R
p38/SAPK2 (Total)
46-612MAG
H,M,R
p53 (Ser15)
46-663MAG
p53 (Total)
46-662MAG
p70S6K (Thr389/412)
46-629MAG
H,M,R
p70S6K (Total)
46-630MAG
H,M,R
Cleaved PARP (Total)
46-656MAG
PTEN (Ser380)
46-679MAG
H,M,R
PTEN (Total)
46-678MAG
H,M,R
RPS6 (Ser235/Ser236)
46-714MAG
H,M,R
RPS6 (Total)
46-715MAG
H,M,R
Src (Tyr419)
46-710MAG
H,M,R
Src (Total)
46-709MAG
H,M,R
STAT1 (Tyr701)
46-655MAG
H,M
STAT1 (Total)
46-654MAG
H,M
STAT3 (Tyr705)
46-623MAG
H,M,R
STAT3 (Ser727)
46-624MAG
H,M,R
STAT3 (Total)
46-625MAG
H,M,R
STAT5A/B (Tyr694/Tyr699)
46-641MAG
H,M,R
Tie2 (pan Tyr)
46-716MAG
Tie2 (Total)
46-717MAG
VEGFR2 (pan Tyr)
46-718MAG
VEGFR2 (Total)
46-719MAG
H Human
AB1: Assay Buffer 1
Acceptable
M Mouse
AB2: Assay Buffer 2
Not Recommended
R Rat
Recommended
INTRACELLU L A R M A Pm a te K I T S
Magnetic Bead MAPmate kits
19
CELL SIG NA LING

Lysates
Cat. No.
Lysate Description
Cat. No.
HeLa: Unstim
47-205
HepG2: Insulin
47-227
Jurkat: Unstim
47-206
HeLa: Lambda Phosphatase
47-229
Jurkat: Anisomycin
47-207
HeLa: TNF+CalA
47-230
Jurkat: H2O2
47-208
HepG2: Unstim (1)
47-231
A431: EGF
47-210
HepG2: DCA
47-232
HeLa:HS/Ars
47-211
HEK293: Serum
47-233
NIH3T3: PDGF-AB
47-213
HepG2:Unstim (2)
47-234
MCF7: IGF-1
47-216
HepG2: TGF
47-235
Daudi: IL-4
47-217
THP-1: Serum
47-236
A549: Camptothecin
47-218
HFF-1: Serum
47-237
NIH3T3: Anisomycin
47-219
HUVEC: Serum
47-238
Jurkat: Paclitaxel
47-220
HepG2: Unstim(3)
47-239
Ramos: PVD
47-224
NTERA-2: Unstim
47-241
HL-60: PVD
47-225
MCF-7: Unstim
47-242
HeLa: IFN
47-226
Rat Heart Lysate
50-100
Lysate Description
20
Luminex Instruments
for xMAP Technology
LU M INEX TECH NO LO GY
Complete instrument solutions, combined with the largest portfolio of multiplex analytes, MILLIPLEX Analyst 5.1
software and technical support, give you the maximum power of Luminex xMAP technology for biomarker screening
and protein analysis. As a Luminex partner, Merck Millipore is a preferred distributor of Luminex instruments,
accessories and software.
MAGPIX System
(Cat. No. 40-072 - MAGPIX)
Low-cost Entry-level platform to measure up to 50 analytes in

as little as 25 L
Powerful - More than 110 (and growing) MILLIPLEX map
magnetic bead kits the largest offering of customizable
magnetic bead assay panels for the MAGPIX instrument
Easy to use - Magnetic bead detection using CCD imaging
Small and portable Saves room on your bench, requiring only
3 ft (91 cm) of space and only minimal setup
Luminex 200 System
FLEXMAP 3D System
(Cat No. 40-012- MILLIPLEX Analyzer)
(Cat. No. 40-014 - FLEXMAP 3D)
Flexible Run both magnetic and

non-magnetic bead assays
Highest multiplexing - Each bead contains different

concentrations of a combination of dyes. Monitoring the three
signals enables discrimination of up to 500 different bead sets
Multiplex Up to 100 analytes per well

of a 96-well plate in as little as 25 L of
sample
Easy to use User-friendly programming
96- and 384-well capability - Greater sample volume flexibility

and increased throughput
INSTRUMENTS
Scalable Bar code reader included
Ultra-fast - Quantify 48,000 analytes in under one hour. Dual

sample fluidics paths and increased syringe injection speed
facilitate faster sample injection
Automation/LIS compatibility - New xPONENT 4.0 Software

controls the system and offers interfacing options for Laboratory
Information Systems (LIS) and automation
For complete information on MILLIPLEX Analyst 5.1 software, BioTek Plate washing solutions, and Advanced
Customer Training (ACT) please see our Luminex xMAP Technology Brochure (Literature Code DS0012ENEU).
21
MILLIPLEX
Analyst 5.1 Software
MI LLI PLEX A NA LYS T 5.1 SO F T WA RE
MILLIPLEX Analyst 5.1

(Cat. No. 40-086 MILLIPLEX Analyst 5.1 1 seat license)
(Cat. No. 40-087 MILLIPLEX Analyst 5.1 5 seat license)
MILLPLEX Analyst software offers

simple integration with the Luminex
xPONENT system
Seamless loading of data via the Quick Start "Wizard"
Automatic importing of data from Luminex
instruments using our novel "Watchdog" feature
Our superior algorithms yield better data from the low
and high ends of the standard curve
Comprehensive, detailed reports and enhanced
visualization
New features and improvements of MILLIPLEX Analyst 5.1 software

Complete with CD and Dongle USB/Thumb drive for quick and easy installation
Auto-detect feature for standards, quality controls, and samples
Easily analyze data from multiple sources including Luminex 1.7 file formats, Microsoft Excel,
Bio-Plex, and other Luminex data analysis-related software programs
SOFTWARE
The best curve fit possible using our unique algorithms

Qualitative analysis allows for relative comparisons between samples and analytes
Curve Potency feature (comparing two or more standard curves)
Create custom formats for importing data
Export compatible: PDF and Microsoft Excel formats
Reports values for CHI, Recovery, and CVs
Even very large data sets are calculated in seconds
Full support of Microsoft Windows 7, 32-bit and 64-bit

Complete support from our world-class technical service team
22
ELIS A / RIA
Single Protein Analysis
Quantify critical targets and interpret cell-based assays using Merck Millipore's highly validated, preconfigured ELISA and
RIA assays and reagents. Our gold standard immunoassays will provide your research team with the sensitivity and
reliability you have come to know and trust. We advance your research in many research areas including metabolic disease
(diabetes, obesity, cardiovascular), inflammation, immunology, neuroscience, oncology and cell signaling. Plus, some of
our most popular ELISAs are available in a 10-pack format.
Cytokine ELISAs
Kit
IL-1
Species
Human
Catalogue No.
EZHIL1B
IL-2
Human
EZHIL2
IL-2
Mouse
EZMIL2
IL-4
Human
EZHIL4
IL-4
Mouse
EZMIL4
Kit
Species
Nerve Growth Factor

(NGF)
Rat/Mouse
Catalogue No.
Neuropeptide Y (NPY)
Human
Neuropeptide Y (NPY)
Rat/Mouse
Phosphorylated
Neurofilament (pNF-H)
Multispecies
NS170
CYT304
EZHNPY-25K
EZRMNPY-27K
IL-6
Human
EZHIL6
IL-6
Mouse
EZMIL6
Pigment Epithelium
Derived Factor (PEDF)
Human
CYT420
IL-6
Rat
EZRIL6
S100B
Human
EZHS100B-33K
IL-8/CXCL8
Human
EZHIL8
IL-10
Human
EZHIL10
IL-10
Mouse
EZMIL10
IL-12 (p70)
Human
EZHIL12P70
IL-13
Human
EZHIL13
IL-15
Human
EZHIL15
IL-17A
Rat
EZRIL17A
ELISA Circulating Biomarkers

Kit
Species
Adiponectin
10 - Pack
Human
Catalogue No.
EZHADP-61K
EZHADP-61BK
Adiponectin
10 - Pack
Mouse
EZMADP-60K
EZMADP-60BK
IL-17F
Mouse
EZMIL17F
Adiponectin
Rat
TNF
Human
EZHTNFA
Adiponectin
Canine
EZCADP63K
TNF
Mouse
EZMTNFA
Human
EZHMWA-64K
TNF
Rat
RZRTNFA
Adiponectin High
Molecular Weight
10 - Pack
Amylin (active)
10 - Pack
Human
EZHA-52K
EZHA-52BK
Apo AIV
Human
EZHAPOA4-73K
BDNF
Human/Rat
Chemerin
Human
EZHCMRN-57K
C-Peptide
10 - Pack
Human
EZHCP-20K
EZHCP-20BK
C-Peptide 2
Rat/Mouse
CRP
Human
CYT298
CRP
Rat
CYT294
Neuroscience ELISAs
Kit
Species
a-Synuclein
Human/Rat/
Mouse
Amyloid Beta, 1-40
Human
Catalogue No.
NS400
EZHS40
EZHS42
EZHS-SET
Amyloid Beta (Brain),

1-40
Human
EZBRAIN40

1-42
Human
EZBRAIN42

SET
Human
EZBRAIN-SET
Brain Derived
Neurotrophic Factor
(BDNF)
Human/Rat
Glial fibrillary acidic

protein (GFAP)
Human/Rat/
Mouse
CYT306
NS830
CYT306
EZRMCP2-21K
ELISA / RIA
Human
Human
EZHMWA-64BK
Download our featured white papers!

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Lit. No. AN4993ENEU
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Amyloid Beta, SET
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Measure Cytokine Levels

Lit. No. AN4323ENEU
Reference the "Lit. No. "when searching online at:
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23
ELIS A / RIA
ELISA Circulating Biomarkers, continued

Kit
Species
CRP
Canine
CYT296
FABP-3
10 - Pack
Human
EZHFABP3
EZHFABP3-BK
Species
Procollagen Type IIA N-Propeptide (PIIANP)
Human
Catalogue No.
EZPIIANP-53K
Proinsulin (Intact)
Human
EZHIPI-17K
Proinsulin (total)
10 - Pack
Human
EZHPI-15K
EZHPI-15BK
PYY (total)
10 - Pack
Human
EZHPYYT66K
EZHPYYT-66BK
RBP4
Human
EZHRBP4-18K
Resistin
10 - Pack
Human
EZHR-95K
EZHR-95BK
SAA-3
Mouse
EZMSAA3-12K
Human
FGF-21
Rat/Mouse
EZRMFGF21-26K
FGF-23
10 - Pack
Human
EZHFGF23-32K
EZHFGF23-32BK
FGF-23
10 - Pack
Rat
EZRFGF23-42K
EZRFGF23-42BK
FGF-23
10 - Pack
Mouse
EZMFGF23-43K
EZMFGF23-43BK
Ghrelin (active)
10 - Pack
Human
EZGRA-88K
EZGRA-88BK
Ghrelin (active)
Rat/Mouse
EZRGRA-90K
Ghrelin (total)
10 - Pack
Human
EZGRT-89K
EZGRT-89BK
Kit
Species
Adiponectin
Human
Ghrelin (total)
Rat
EZRGRT-91K
C-Peptide
Human
HCP-20K
GIP (total)
10 - Pack
Human
EZHGIP-54K
EZHGIP-54BK
C-Peptide
Canine
CCP-24HK
GIP (total)
10 - Pack
Rat/Mouse
Ghrelin (active)
Human
GHRA-88HK
Ghrelin (total)
Human
GLP-1 (active)
Multi-species
GLP1A-35HK
GLP-1 (total)
Multi-species
GLP1T-36HK
Glucagon
Multi-species
GL-32K
Insulin
Rat
RI-13K
Insulin
Porcine
EZRMGIP-55K
EZRMGIP-55BK
GLP-1 (active)
10 - Pack
Multi-species
EGLP-35K
EGLP-35BK
GLP-1 High Sensitivity

(active)
10 - Pack
Multi-species
EZGLPHS-35K
GLP-1 (total)
10 - Pack
Multi-species
GLP-2 (total)
10 - Pack
Human/Rat
Glucagon n
Human/Rat/
Mouse
EZGLPHS-35BK
EZGLP1T-36K
EZGLP1T-36BK
Insulin Lispro
Catalogue No.
HADP-61HK
GHRT-89HK
PI-12K
LPI-16K
Human
Insulin
Rat
EZGLU-30K
Leptin
Human
EZGLU-30BK
Leptin
Multi-species
EZRMGH-45K
EZRMGH-45BK
Proinsulin
Human
HPI-15K
PYY (3-36) Specific
Human
PYY-67HK
PYY (total)
Human
PYYT-66HK
Rat/Mouse
IFN
Human
Cyt271
Insulin
10 - Pack
Human
EZHI-14K
EZHI-14BK
Insulin
10 - Pack
Rat/Mouse
EZRMI-13K
EZRMI-13BK
EZHIASF-14K
Laminin
Human
ECM310
Leptin Dual Range

10 - Pack
Human
EZHL-80SK
EZHL-80BK
Leptin
10 - Pack
Mouse
EZML-82K
EZML-82BK
Leptin
10 - Pack
Rat
EZRL-83K
EZRL-83BK
Leptin
Canine
EZCL-31K
Lipocalin-2/NGAL
Mouse
EZMNGAL
Omentin-1
Human
EZH0MNTN1-29K
Pancreatic Polypeptide
10 - Pack
Human
EZHPP40K
EZHPP-40BK
RIA
Insulin Specific
EZGLP2-37K
EZGLP2-37BK
Growth Hormone
10 - Pack
Insulin (Animal Serum Free) Human
ELISA / RIA
EZHFGF21-19K
Kit
FGF-21
10 - Pack
24
Catalogue No.
Solid Plate Format

n Requires Sample Extraction
HI-14K
SRI-13K
HL-81K
XL-85K
Goodbye,
Bradford Assays!
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W
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IR spectrometry measures amide bond absorbance for
better accuracy across all proteins and peptides
C
an detect other biomolecules, such as lipids, for
analyzing complex mixtures
Direct Detect Spectrometer

and Starter Kit
R
equires minimal sample volume (2 L), preserving
precious samples
(Cat. No. DDHW00010-WW)
Ordering Information
Description
Direct Detect Spectrometer and Starter Kit
Qty
Catalogue No.
DDHW00010-WW
Includes
Direct Detect Spectrometer
Universal Power Adapter
Dell Latitude 2120 Netbook and power adapter
Direct Detect Software
Netbook Stand
Spotting Tray
Ethernet Cable
Direct Detect Assay-free Cards (50/pk)
1
1
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DDAC00010-4P
DDAC00010-8P
Quit Assays ForeverQuantitate Directly.

Visit: merckmillipore.com/directdetect
25
DIRECT DETECT
To Place an Order or Receive

Technical Assistance
In Europe, please call Customer Service:
France: 0825 045 645

Germany: 069 86798021
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For other countries across Europe,
please call: +44 (0) 115 943 0840
Or visit: www.merckmillipore.com/offices
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Get Connected!
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Join Merck Millipore bioscience on your favorite social

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Merck Millipore, the M mark, Direct Detect and MILLIPLEX are registered trademarks of Merck KGaA, Darmstadt, Germany.
MAPmate is a trademark of Merck KGaA, Darmstadt, Germany.
All other trademarks belonging to third parties are the properties of their respective owners.
Lit. No. PF1089ENEU Rev. V 06/2014 MIL-180 BS GEN-14-10262-EX
2014 EMD Millipore Corporation, Billerica, MA, USA. All Rights Reserved.

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Analyte Quarterly, Vol. 2 2014

Discover the Latest in Innovative and Unique Multiplex

For Research Use Only.

Kidney Toxicity Panel 2

Soluble Cytokine Receptor

Soluble Cytokine Receptor

STAT3 Phospho/Total 2 Plex

Vascular Injury Panel 1

Vascular Injury Panel 2

Vascular Injury Panel 3

Kidney Injury Panel 1

Kidney Injury Panel 2

Kidney Injury Panel 3

Kidney Injury Panel 4

Kidney Injury Panel 5

Kidney Injury Panel 6

Kidney Injury Panel 1

Kidney Injury Panel 2

Kidney Toxicity Expanded

Online Kit Ordering Is Faster

Order your MILLIPEX map panels and kits online.

MILLIPLEX MAP New Product

Compared to the traditionally used rats, using a mouse

Here, we show a subset of the multiplex assay

Figure 1. Protein Biomarkers in Cisplatin-Induced Kidney Injury Mouse Model.

For Research Use Only.

Bring your biomarkers to life.

We build custom kits to meet your

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Advantages of using Merck Millipore

High correlation to ELISA data.

Merck Millipore has over 25 years experience in

GyroMark HT Kitseverything you need in one, easy-to-use format:

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Biomarker Wish List

Human Circulating Cancer

(Cat. No. HBNMAG-51K)

(Cat. No. HCCBP3MAG-58K)

(Cat. No. HCVD1MAG-67K)

Fibroblast activation protein (FAP)

Melanoma inhibitory activity (MIA)

Human Single Plex Bone Kits

Sex hormone binding globulin (SHBG)

Human Cancer / Metastasis

Human Circulating Cancer

(Cat. No. HCMBMAG-22K)

Human Angiogenesis / Growth

Human CVD Panel 3 (Acute

Human CVD Panel 4

Tissue Factor (TF)

Complement factor H (CFH)

(Cat. No. HCVD2MAG-67K)

Human CVD Panel 2

(Cat. No. HCCBP2MAG-58K)

Vitamin D binding protein

Human CVD Panel 1

For Research Use Only.

Human Cytokine / Chemokine

Human Cytokine / Chemokine

(Cat. No. HCYTOMAG-60K)

(Cat. No. HCYP3MAG-63K)

Fully qualified, robust and reproducible assays

Merck Millipore has over 25 years experience in

% Requires a protease inhibitor during