DOI 10.1007/s11947-007-0014-1
Received: 31 May 2007 / Accepted: 1 August 2007 / Published online: 14 September 2007
# Springer Science + Business Media, LLC 2007
Introduction
An edible film is a continuous thin layer of edible polymer,
which can be applied as a coating or used as a standalone
membrane for improving food quality. It is often used to
preclude unwanted mass transports (e.g., moisture, oxygen,
aroma, and flavor), enhance visual attributes (e.g., gloss and
color), and function as a carrier to deliver active materials
(e.g., antimicrobial agents and nutraceuticals). Over the past
decade, edible films have been subjected to intensive
research because of their potential for reducing the use of
synthetic thermoplastics in food packaging.
Edible films can be derived from proteins, polysaccharides, lipids, and resins. By and large, proteins have received
the most attention in edible film research because of their
abundance as agricultural by-products and food processing
residuals. The presence of reactive amino acid residuals
also enable protein to be modified and crosslinked through
physical and chemical treatments to produce novel polymeric structures (Gennadios 2002; Lundblad 2005). Whey
proteins from bovine milk have been studied to a great
extent because of their ability to form transparent and
flexible films, which exhibit good barrier and mechanical
properties (Krochta 2002). Whey proteins are globular
proteins, which remain soluble after precipitation of casein
at pH 4.6 during cheese making. In bovine milk, these
thermal-labile proteins consist of mainly -lactalbumin, lactoglobulin, and other proteins present in smaller fractions
(e.g., bovine serum albumin, immunoglobulins, proteasepeptones). Whey proteins are commercially available as
315
316
Flaking
Cracking
Blistering
None
No visual
evidence of
flaking
One spot is
affected with an
area less than
10% of the
plum surface
area
More than one
spot where
flaking
occurred but
affects less than
20% of the
plum surface
area
More than one
spot where
flaking
occurred that
affects more
than 20% of the
plum surface
area
No visual
evidence of
cracking
Presence of a
small crack
No visual
evidence of
blistering
Presence of a
small blister
(<1 mm)
Small cracks
that affect less
than 20% of
the plum
surface area
Presence of
blisters that
affect less than
20% of the
plum surface
area
Cracks affect
more than
20% of the
plum surface
area
Presence of
blisters that
affect more
than 20% of the
plum surface
area
Slight
Coating Procedure
Plums were previously washed using tap water and then
allowed to dry at room temperature. Coating was applied by
dipping each plum into the solution for 5 min and drained.
After the coating process, plums were dried using a fan
(Airworks Model FFH2, Markham, ON, Canada) at an air
velocity of 1.5 m/s for 30 min at 212C. Coated plums
were stored at 5C for 15 days.
Moderate
High
Film Casting
Film-forming solutions were cast on Plexiglas plates of
1730 cm. The total solids content per area was maintained
at 0.028 g/cm2 to minimize thickness variations. Cast
solutions were allowed to dry on a leveled granite surface
for 48 h at 233C. Dried films were then peeled intact.
Tests on Coated Plums
Firmness
Coating Stability
WPI
Protein
GLY
FS
Emulsifier (Tween 60)
BW
WPI (%)
10
9.1
9
0.0
0.20
0.00
WPI-5FS
(%)
WPI-10FS
(%)
9.2
8.6
9
5.0
0.20
0.50
8.7
8.1
8
10.0
0.20
1.00
Mass Loss
Sample sizes of 18 fruits per treatment, including the
control samples, were used for mass loss evaluation. The
plums were weighed at 0, 2, 6, 9, 12, and 15 days during
storage. The mass loss was expressed as percentage loss of
initial mass. The precision balance used for weight
measurement had an accuracy of 0.1 mg.
Sensory Analysis
Sensory evaluation of the coated and control samples was
conducted on the 15th day of storage using a sensory panel
consisted of 29 individuals with age ranging from 20 to
45 years old. Panel members were previously screened for
allergic reactions to the ingredients used in the coatings.
Samples were removed from refrigeration 1 h before the
sensory evaluation to allow them to equilibrate to room
temperature. Coded samples were presented to the panelist
who was asked to score appearance, overall impression,
flavor, sweetness, and firmness using a hedonic scale of 0
10 (0dislike; 10like). The panel was also asked if the
use of edible films and the incorporation of nutraceuticals
in coating would influence their purchase decision.
317
OTR
:t
p
Tests on Films
Tensile Properties
Statistical Analysis
318
319
320
Force (N)
Uncoated plums
WPI-Pa
WPI
WPI-5FS
WPI-10FS
6.50.8b
6.01.4b
8.52.0a
6.51.1b
6.00.9b
Tensile strength
(MPa)
Elongation at
break (%)
WPI
WPI-5FS
WPI-10FS
0.229
0.280
0.266
6.100.18a
1.840.24b
1.830.51b
19.491.2c
12.042.8d
7.501.2e
Means with the same letters in a column are not significantly different.
Film
4.5
4.0
Control
WPI
3.5
WPI-5FS
WPI-10FS
3.0
2.5
2.0
1.5
1.0
0.5
0.0
0
8
Time [days]
10
12
14
16
4
WPI
3.5
WPI-5FS
321
WPI-10FS
3
2.5
2
1.5
1
0.5
0
25
35
45
55
65
75
Table 5 Comparison of water vapor permeability values of WPI and synthetic films
Film
Test conditions
Permeability (g mm/m2
h kPa)
Source
WPI:GLY (1:1)
WPI:GLY:FS:BW: (1:1:0.6:0.06)
WPI:GLY:FS:BW: (1:1:1.24:0.124)
WPI:GLY 1:1
WPI:BW:GLY 1:1:1
WPI:BW:GLY 3:3:1
Beeswax
PVC
LDPE
23C,
23C,
23C,
23C,
23C,
23C,
26C,
26C,
26C,
1.9600.024a
1.2100.094b
0.5700.056c
2.5700.34
1.5100.27
0.1300.09
0.004
0.026
0.001
Current study
43% RH
43% RH
43% RH
4020%
4020%
4020%
100% RH
100% RH
100% RH
Current study values are means of three replicates. Means with the different letters are significantly different at 5% level.
WPI Whey protein isolate, GLY glycerol, FS flaxseed oil, BW beeswax
322
c
Fig. 8 Appearance of films after water vapor transmission test. a WPI
films, b WPI-5FS films, and c WPI-10FS films. Samples on the left
and right were exposed to 75 and 30% RH for 86 h, respectively.
Refer to Table 1 for formulation codes
Film
Test conditions
Permeability coefficient
(cm3 m/m2 d kPa]
Source
WPI:GLY(1:1)
WPI:GLY:FS:BW: (1:1:0.6:0.06)
WPI:GLY:FS:BW: (1:1:1.24:0.124)
WPI:Gly (2.3:1)
LDPE
HDPE
EVOH (70% VOH)
PVDC-Based films
23C;
23C;
23C;
23C;
23C;
23C;
23C;
23C;
120.010.0a
278.410.1b
525.740.3c
76.1
1870.0
427.0
12.0
0.45.1
Current study
50%
50%
50%
50%
50%
50%
50%
50%
RH
RH
RH
RH
RH
RH
RH
RH
323
Fig. 9 Results of sensory evaluation of plums coated with WPI and WPI-FS formulations after 15 days of storage at 5C. Average scores are
presented in the box located in each figure. Refer to Table 1 for formulation codes
Often
(15%)
Occasionally
(27%)
Yes,
increase
(73%)
Yes,
increase
(31%)
Yes,
increase
(77%)
No (12%)
Would it affect your buying decision to know that the plums were added
with a nutritional substance?
Would it affect your purchasing decision if you knew in advance that the
plums were coated with natural ingredients to extend shelf-life?
No (38%)
No (12%)
Only in
season
(27%)
Do not
care
(15%)
Do not
care
(27%)
Do not
care
(12%)
Once in a
while
(31%)
Never
(0%)
324
Conclusions
The addition of flaxseed oil and beeswax in WPI film
reduced mass loss from plums by providing a better water
vapor barrier. Shelf life of plums can be extended because
phenomena associated with mass loss such as shriveling
and firmness loss were delayed. Coating of plums with WPI
films enhanced appearance of plums by creating glossy
surfaces. Although the presence of beeswax decreased
glossiness, a better appearance was observed in comparison
with the uncoated plums. Overall, coating of plum did not
impact the perceived flavor and firmness of the plums.
Plums coated with WPI showed least significant coating
defects over a storage period of 15 days at 5C. WPI-10FS
formulation also exhibited a low incidence of defects,
making this coating stable as well. The addition of a lipid
phase consisting of 5% flaxseed oil and 0.5% beeswax to
the WPI matrix compromised the film physical stability by
the formation of defects that weakens its barrier properties.
This study confirmed the feasibility of applying edible
films containing flaxseed oil to enhance the nutritional
value of plums; however, flaxseed oil migration to the
surface pose important stability issues that need to be
addressed. Future studies should take this into consideration
to determine the ability of the WPI films to preserve the
stability of the added nutraceutical.
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