Abstract
Microsporum canis has been frequently isolated from
human cases of tinea capitis and tinea corporis. The
infection may be acquired from infected animals with
cutaneous lesions but also from asymptomatic carriers or from the environment. As asymptomatic M.
canis carriers are considered to be a critical factor in
the epidemiology of dermatophytosis in humans, this
study investigated the relationship between the presence of dermatophytes on the hair coats of dogs and
cats without cutaneous lesions and the occurrence of
the disease in their respective owners. A total of 136
dogs and 248 cats were sampled from January 1999 to
Introduction
Dermatophytosis is a common infection of keratinized
tissues (e.g. skin, nails and hair) characterized by multifocal alopecia, scaling and circular lesions. Microsporum and
Trichophyton species are responsible for infections in
animals and Microsporum canis is the most commonly
isolated dermatophyte from dogs and cats with or without
cutaneous lesions.14 Microsporum gypseum, Trichophyton terrestre and Trichophyton ajelloi are geophilic dermatophytes mainly isolated from dogs and cats without
lesions;2,5 however, the pathogenic role of these geophilic
species is still a matter of controversy. The transmission
of M. canis occurs via infective arthrospores present on
the hair coats of dogs and cats or in the environment.1
Humans may be infected, and M. canis has become the
most frequently encountered zoonotic dermatophyte in
urban areas.6,7
The prevalence of M. canis infections in humans differs
from one country to another.8 In Italy, it is the most common dermatophyte isolated from tinea capitis and tinea
corporis cases.9,10 This may be explained by the great
number of dogs and cats kept as pets in this country.911
Asymptomatic animal carriers of M. canis are considered
to be a critical factor in the epidemiology of the disease as
50% of humans exposed to infected asymptomatic animals, especially cats, may become infected.12 The preva-
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Cafarchia et al.
Dogs
(n = 136)
Cats
(n = 248)
Total
Microsporum canis
Microsporum gypseum
Trichophyton terrestre
Trichophyton ajelloi
Total
8 (5.9%)
10 (7.4%)
12 (8.8%)
0
28* (20.5%)
58 (23.4%)
4 (1.6%)
6 (2.4%)
2 (0.8%)
70 (28.2%)
66 (17.2%)
14 (3.6%)
18 (4.7%)
2 (0.5%)
98* (25.5%)
*Two dogs were positive for two dermatophyte species (M. canis and
M. gypseum).
Group B
8/22 (36.4%)
30/56 (53.6%)
38/78 (49%)
0/114 (0%)
28/192 (14.6%)
28/306 (9%)
Statistical analysis
Differences of M. canis prevalence in groups A and B were tested by
Fisher exact test. Epidemiological data from dogs and cats were analysed separately using multivariate logistic regression models19 to
evaluate risk factors associated with the presence of dermatophytes.
Significant risk factors were presented as odds ratio (OR) and 95%
confidence interval (CI). For statistical purposes, only prevalences
higher than 10% were considered. Consequently, in logistic regression the dependent variables were positive cultures (yes/no) for M.
canis in cats and positive cultures for geophilic dermatophytes in
dogs. Independent variables were the data listed in Tables 3 and 4.
Co-linearity among independent variables was controlled using the
Pearson correlation coefficient. None of the variables presented a
high correlation (correlation coefficients less than 0.38) so all epidemiological data were introduced into the model. All data were analysed
using SPSS for Windows (version 12.01, SPSS, Inc., Chicago, IL,
USA). A P < 0.05 was considered significant.
Results
Sampling procedures
Hair samples were collected using the brush technique.16 Each animal
was entirely brushed using an 8 cm diameter plastic brush for at least
3 min starting from the head followed by the neck, dorsum, trunk,
ventrum, limbs and tail. After specimen collection, the brush was
placed in its original package and transported as soon as possible to
the laboratory for dermatophyte culture.
Fungal culture
Fungal cultures were performed by pressing the bristles of the brush
onto 90 mm Petri dishes containing Sabouraud agar with chloramphenicol (0.5%) and actidione (0.4%) (Liofilchem Diagnostici,
Roseto degli Abruzzi, Italy) and incubated at 25 C for 15 days. Colonies grown in the medium were identified to species based on their
morphology and microscopic characteristic of the hyphae, macroconidia and microconidia as described by Rebell and Taplin17 and de
Hoog.18
328
Dogs
Cats
Total
Group A
2006 The Authors. Journal compilation 2006 European Society of Veterinary Dermatology.
Cats
4/80 (5.0%)
4/56 (7.1%)
32/128 (25.0%)
26/120 (21.7%)
0/44 (0%)
4/48 (8.3%)
4/44 (9.1%)
26/82 (31.6%)
18/92 (19.5%)
14/74 (18.9%)
2/68 (2.9%)
6/68 (8.8%)
26/94 (27.7%)
32/154 (20.8%)
4/36 (11.1%)
4/100 (4.0%)
4/98 (4.1%)
54/150 (36.0%)
2/30 (6.6%)
4/28 (14.3%)
0/68 (0%)
2/10 (20.0%)
18/116 (15.5%)
4/52 (7.7%)
21/53 (39.6%)
15/27 (55.6%)
Dogs
Sex
Female
Male
Age*
< 1 years
15 years
> 5 years
Breed
Cross-breed
Pure breed
Habitat
Urban
Rural
Season
Spring
Summer
Fall
Winter
Cats
14/80 (17.5%)
8/56 (14.2%)
0/128 (0%)
10/120 (8.3%)
14/44 (31.8%)
4/48 (8.3%)
2/44 (4.5%)
4/82 (4.9%)
6/92 (6.5%)
0/74 (0%)
2/68 (2.9%)
20/68 (29.4%)
6/94 (6.4%)
4/154 (2.6%)
2/36 (5.5%)
20/100 (20.0%)
2/150 (1.3%)
8/98 (8.2%)
4/30 (13.3%)
6/28 (21.4%)
12/68 (17.6%)
0/10 (0%)
6/116 (5.2%)
2/52 (3.8%)
2/53 (3.8%)
0/27 (0%)
*Age has not been reported for two dogs and for two cats.
(OR = 4.233; CI = 1.956 9.160) proved to act as predisposing factors for M. canis carriage in cats (P < 0.001),
whereas age (OR = 2.503; CI = 1.204 5.202) and breed
(OR = 3.208; CI = 1.496 6.878) were risk factors for
geophilic dermatophyte infections in dogs (P < 0.001). In
other words, cats living in a rural environment and those
< 1 year of age sampled in the winter months were more
likely to carry M. canis, whereas no differences were
observed according to sex or breed. Dogs < 1 year of age
and pure breed dogs showed a higher prevalence of
geophilic species carriage than other dogs (Table 4). No
geophilic dermatophytes were isolated from female cats
or from cats older than 5 years nor were they isolated from
dogs or cats in the winter months.
Discussion
The role of cats as asymptomatic carriers of M. canis has
been extensively described by many investigatiors8,20 and
cats are now recognized as major sources of infection
to other animals and their owners. The role of cats in
human infection may be related to the greater number of
spores shed in the environment by infected cats than that
shed by dogs.12 The present study clearly indicated that
asymptomatic dogs could also carry pathogenic dermatophytes (M. canis and geophilic species) on their hair coats.
Microsporum canis was more frequently isolated from the
hair coats of dogs and cats cohabiting with owners diagnosed with M. canis tinea corporis. However, it was also
present on the hair coat of some cats cohabiting with
healthy owners but they were never isolated from dogs
living with healthy owners. These results seem to indicate
that many dogs examined in the present study were not
responsible for the transmission of M. canis to humans,
and suggest that cats were the source of transmission
of M. canis to humans. When M. canis was detected
on the coat of asymptomatic dogs, their owners consistently had M. canis tinea corporis. However, when dog
owners had dermatophytosis, the dogs were more
frequently culture negative (14 dogs) than culture positive
2006 The Authors. Journal compilation 2006 European Society of Veterinary Dermatology.
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Cafarchia et al.
References
1. Sparkes AH, Werrett G, Stokes CR et al. Microsporum canis: inapparent carriage by cats and the viability of arthrospores. Journal
of Small Animal Practice 1994; 35: 397401.
2. Cabanes FJ, Abarca ML, Bragulat MR et al. Seasonal study of
fungal biota of the fur of dogs. Mycopathologia 1996; 133: 17.
3. Cafarchia C, Romito D, Sasanelli M et al. The epidemiology of
canine and feline dermatophytoses in southern Italy. Mycoses
2004; 47: 50813.
4. Patel A, Lloyd DH, Lamport AI. Survey of dermatophytes on clinically normal cats in the southeast of England. The Journal of
Small Animal Practice 2005; 46: 4369.
5. Moriello KA, DeBoer DJ. Fungal flora of the coat of pet cats.
American Journal of Veterinary Research 1991; 52: 6026.
6. Lunder M, Lunder M. Is Microsporum canis about to become a
serious dermatological problem? Dermatology 1992; 184: 879.
Rsum Microsporum canis a frquemment t isol des cas tinea capitis et de tinea corporis chez
lhomme. Linfection peut tre acquise partir dun animal infect avec des lsions cutanes, mais galement partir dun porteur asymptomatique ou de lenvironnement. Comme les porteurs asymptomatiques
sont considrs critiques dans lpidmiologie des dermatophytoses humaines, cette tude sest
intresse la relation entre la prsence de dermatophytes sur le pelage de chiens et de chats sans lsion
cutane et lapparition de lsions chez leurs propritaires. Au total, 136 chiens et 248 chats ont t prlevs
entre janvier 1999 et janvier 2005. Soixante dix huit animaux (22 chiens et 56 chats) appartenaient des
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2006 The Authors. Journal compilation 2006 European Society of Veterinary Dermatology.
propritaires souffrant de tinea corporis due M. canis et 306 (114 chiens et 192 chats) des propritaires
sans dermatophytose. Lge, le sexe, la race, lhabitat et la saison ont t tudies pour chaque animal et
examins comme risque potentiel. Des dermatophytes ont t isols de 20.5% des chiens et 28.2% des
chats. M. canis a t isol de 36.4% des chiens cohabitant avec un propritaire prsentant une tinea corporis
mais jamais chez les chiens dont les propritaires ne prsentaient pas de lsion. A loppos, M. canis a
t isol de 53.6% des chats cohabitant avec un propritaire souffrant de tinea corporis et de 14.6% des
chats dont le propritaire ne prsentait pas de lsion. Ces rsultats indiquent clairement que chiens et chats
doivent tre considrs comme des sources majeures de dermatophytes pathognes pour lhumain, mme
sans signe clinique de dermatophytose.
Resumen Microsporum canis se ha aislado con frecuencia en casos humanos de tinea capitis y tinea
corporis. La infeccin puede adquirirse de animales infectados y con lesiones cutneas, pero tambin de
portadores asintomticos o del ambiente. Ya que los portadores asintomticos de M. canis se consideran
un factor crtico en la epidemiologa de la dermatofitosis humana, en este estudio investigamos la relacin
entre la presencia de dermatofitos en el pelo de perros y gatos sin lesiones cutneas, y la presencia de la
enfermedad en los respectivos dueos. Se tomaron muestras de un total de 136 perros y 248 gatos entre
Enero de 1999 y Enero del 2005. Setenta y ocho animales (22 perros y 56 gatos) pertenecieron a dueos
afectados con tinea corporis causada por M. canis y 306 (114 perros y 192 gatos) a individuos sin dermatofitosis. Edad, gnero, raza, habitat y estacin se anotaron para cada animal y se examinaron como potenciales
factores de riesgo. Se aislaron dermatofitos de un 20.5% de los perros y de un 28.2% de los gatos. M. canis
se aisl de un 36.4% de los perros conviviendo con dueos diagnosticados con tinea corporis, pero nunca
se aisl en perros cuyos dueos no tenan lesiones. En contraste, M. canis se aisl de un 53.6% de gatos
conviviendo con propietarios diagnosticados con tinea corporis y de un 14.6% de gatos cuyos propietarios
no presentaron enfermedad. Estos resultados claramente demuestran que tanto perros como gatos deben
ser considerados una fuente importante de dermatofitos patgenos para humanos, incluso si no presentan
signos clnicos de dermatofitosis.
Zusammenfassung Microsporum canis wurde hufig bei humanen Fllen von Tinea capitis und Tinea
corporis isoliert. Die Infektion kann von einem infizierten Tier mit Hautlsionen stammen, kann aber
auch von asymptomatischen Trgern oder aus der Umgebung kommen. Da asymptomatische M. canis
Trger als kritische Faktoren bei der Epidemiologie von Dermatophytosen beim Menschen betrachtet
werden, wurde in dieser Studie der Zusammenhang zwischen dem Vorhandensein von Dermatophyten im
Haarkleid von Hunden und Katzen ohne kutane Vernderungen und dem Auftreten der Erkrankung bei
den jeweiligen Besitzern untersucht. Insgesamt wurden von Jnner 1999 bis Jnner 2005 Proben von 136
Hunden und 248 Katzen genommen. Siebenundachtzig Tiere (22 Hunde und 56 Katzen) gehrten Personen, die
an Tinea corporis verursacht durch M. canis, litten und 306 (114 Hunde und 192 Katzen) gehrten Individuen
ohne Dermatophytose. Alter, Geschlecht, Rasse, Haltung und Jahreszeit wurden fr jedes Tier festgehalten
und als mgliche Risikofaktoren untersucht. Dermatophyten wurden bei 20.5% der Hunde und 28.2% der
Katzen isoliert. M. canis wurde bei 36.4% der Hunde isoliert, die mit Besitzern lebten, bei denen Tinea
corporis diagnostiziert worden war. M. canis wurde aber nie bei Hunden isoliert, deren Besitzer keine
Hautlsionen zeigten. Im Gegensatz dazu wurde M. canis bei 53.6% der Katzen isoliert, die bei Besitzern
mit diagnostizierter Tinea corporis lebten und bei 14.6% der Katzen, deren Besitzer keine Anzeichen der
Krankheit aufwiesen. Diese Ergebnisse zeigen deutlich, dass sowohl Katzen als auch Hunde eine bedeutende Quelle fr pathogene Dermatophyten fr den Menschen darstellen, auch wenn sie keine klinischen
Anzeichen von Dermatophytose aufweisen.
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