Antibacterial Activity of Litsea Glutinosa Bark

Fitoterapia 71 2000.
439441
Short report
Antibacterial activity of Litsea glutinosa

bark
Subhash C. Mandal a,U , C.K. Ashok Kumar a ,
Avijit Majumder b, Rupa Majumder b, B.C. Maity c
a
Di ision of Pharmacognosy, Department of Pharmaceutical Technology, Faculty of Engineering

and Technology, Jada pur Uni ersity, Calcutta 700 032, India
b
Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra-835 215, Ranchi,
India
c
Indian Institute of Chemical Biology, 4, Raja S.C. Mallick Road, Jada pur, Calcutta 700 032,
India
Received 13 October 1999; accepted in revised form 29 December 1999
Abstract
The methanol extract of the bark of Litsea glutinosa showed antibacterial activity,
comparable to chloramphenicol, against all 16 tested microorganisms. 2000 Elsevier
Science B.V. All rights reserved.
Keywords: Litsea glutinosa; Antibacterial activity
Plant. Litsea glutinosa Lour. Lauraceae ., bark collected from Hetyasole, Bankura,
West Bengal, India, in August 1997 and identified Ref. No. BSIrCNHrTech.II.
by Dr G.S. Giri of Botanical Survey of India, Shibpur, Howrah. A voucher
specimen has been deposited in the Pharmacognosy Division of the Department of
Pharmaceutical Technology, Jadavpur University, Calcutta.
Uses in traditional medicine. The bark of L. glutinosa is widely used as a
demulcent. Ground and pasted material is used as an emollient. Mucilaginous bark
U
Corresponding author. Fax: q91-334720964.

E-mail address: madhabi@giasc101.vsnl.net.in S.C. Mandal..
0367-326Xr00r$ - see front matter 2000 Elsevier Science B.V. All rights reserved.
PII: S 0 3 6 7 - 3 2 6 X 0 0 . 0 0 1 3 2 - 5
S.C. Mandal et al. r Fitoterapia 71 (2000) 439441
440
Table 1
Antibacterial activity of Litsea glutinosa bark methanol extract a
Microorganisms
Extract grml.
50
Staphylococcus aureus
ML 267
100
Chloramphenicol grml.
150
200
50
100
150
200
6.50
8.00
8.50
9.00
8.50
9.00
9.50
10.00
7.00
8.00
9.00
10.50
9.00
9.50
10.00
12.00
Bacillus subtilis
CDr99r1
7.00
8.00
9.50
10.00
9.00
9.50
9.50
11.00
Lactobacillus arabinosus
CDr99r1
7.00
8.00
9.00
10.00
8.50
9.50
10.00
11.00
Bacillus cereus
V. mycoides
7.00
8.50
10.00
13.50
6.50
8.00
8.50
9.50
Klebsiella pneumoniae
RM8r98
7.00
8.00
8.50
9.50
7.00
8.00
8.50
9.00
Streptococcus pneumoniae
7465
7.00
7.50
8.50
9.00
7.00
8.00
9.00
10.00
Sarcina lutea
CDr99r1
7.00
8.00
8.50
9.00
6.50
8.00
8.50
8.75
Shigella dysenteriae
1
7.50
8.50
9.50
10.00
7.00
8.00
9.00
10.00
Shigella sonnei
2
7.00
9.00
11.00
12.00
7.00
9.50
13.00
16.00
Escherichia coli
Row 7r12
7.50
8.00
9.50
10.00
8.00
8.50
9.50
10.50
E. coli
AM8r98
8.00
8.50
9.50
11.00
8.00
9.00
9.50
10.50
E. coli
VC Sona wave 3: 37C
9.00
10.50
11.50
12.50
8.50
9.00
9.50
10.00
Salmonella typhimurium
2
8.00
8.50
9.00
9.50
8.00
8.50
9.00
9.50
Proteus mirabilis
AM8r98
7.50
9.00
9.50
10.50
7.00
8.00
8.50
9.00
Vibrio cholerae
865
9.50
10.00
10.50
11.00
7.50
8.00
9.00
10.00
Bacillus pumilus
8241
Values are zone of inhibition mm.; tests were done in triplicate.
S.C. Mandal et al. r Fitoterapia 71 (2000) 439441
441
is used in diarrhoea and dysentery treatment, and also for sprains, bruises and
rheumatic gouty joints w1,2x.
Previously isolated constituents. No report.
Tested material. Methanol extract yield: 7.5%, on air-dried bark.. Phytochemical
screening w35x gave positive tests for alkaloids, steroids, triterpenoids, saponins,
and tannins.
Studied activity. Antibacterial activity by an agar diffusion method w6x.
Used microorganisms. Staphylococcus aureus ML 267., Bacillus pumilus 8241.,
Streptococcus pneumoniae 7465., Escherichia coli Row 7r12. obtained from the
Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur,
Calcutta, India. Bacillus subtilis CDr99r1., Lactobacillus arabinosus CDr99r1.,
Bacillus cereus V. mycoides., Sarcina lutea CDr99r1., Shigella dysenteriae 1.,
Shigella sonnei 2., Salmonella typhimurium 2., Vibrio cholerae 865. obtained from
the Central Drug Laboratory, Calcutta, India. Klebsiella pneumoniae RM8r98., E.
coli AM8r98., E. coli VC Sona wave 3: 37C., Proteus mirabilis AM8r98.
obtained from Institute of Microbial Technology, Chandigarh, India.
Results. Reported in Table 1.
Conclusions. The methanol extract of Litsea glutinosa bark effectively inhibited
both gram-positive and gram-negative bacteria. The results justify the reported
uses in diarrhoea and dysentery in traditional medicine.
Acknowledgements
The authors are grateful to Mr Manoranjan Mandal of Hetyasole, Bankura,
West Bengal, India for collection of plant material.
References
w1x
w2x
w3x
w4x
w5x
w6x
The wealth of India. New Delhi: CSIR, 1962;6:153.

The useful plants of India. New Delhi: CSIR, 1986:334.
Trease GE, Evans WC. Pharmacognosy. 12th ed. ELBS Publication, 1985:539.
Libermann C. Ber 1885;18:1803.
Noller CR, Smith RA, Harris GR, Walker JW. J Am Chem Soc 1942;64:3047.
Mutreja HC, Saharia GS, Sharma HS. Ind J Pharm Sci 1981;43:6.

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Fitoterapia 71 2000.

Antibacterial activity of Litsea glutinosa

Di ision of Pharmacognosy, Department of Pharmaceutical Technology, Faculty of Engineering

Received 13 October 1999; accepted in revised form 29 December 1999

Corresponding author. Fax: q91-334720964.

S.C. Mandal et al. r Fitoterapia 71 (2000) 439441

Values are zone of inhibition mm.; tests were done in triplicate.

S.C. Mandal et al. r Fitoterapia 71 (2000) 439441

The wealth of India. New Delhi: CSIR, 1962;6:153.

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