Original Article
Faculty of Agro-Industry,
Abstract
Respiration rate at 4oC and minimal processing of rambutan cv. Rong-Rien were investigated. Rambutan was
harvested from Amphur Ban Na San, Surat Thani Province, at the stage when its skin was turning into a combination of red,
green and yellow. After harvesting, the fruits were size-graded to 27-30 fruits/kg, hydrocooled to 14oC, packed with ice in
Styrofoam boxes and transported to the laboratory at Prince of Songkla University within 6 h. The respiration rate of fresh
rambutan fruits was monitored. For minimal processing, the fruits were soaked in warm solution (55oC) of 100 ppm sodium
hypochlorite for one min and immediately cooled in cold water until their internal temperature reached 14oC. The minimal
process included peeling, with and without coring. The peeled and peeled and cored rambutan samples were immersed in a
solution of 0.5% citric acid + 0.5% CaCl2 at 4oC for 2 min. The average respiration rates (within 6 h) at 4oC of whole fruit,
peeled, and peeled and cored rambutan samples were measured and found to be 122, 134 and 143 mg CO2/kg/h, respectively. These findings indicated that a preparation style as peeled rambutan without coring, nylon/LLDPE bag, storage
temperature of 4.01oC, were suitably applied for processed rambutans. To obtain a longer extended shelf life (>12 days) of
minimally processed peeled rambutans, further study on food additives, including acidulants and preservative used and gas
composition in modified atmosphere packaging (MAP) is needed.
Keywords: respiration rate, rambutan, rambutan cv.Rong-Rien, minimally processed rambutan,
modified atmosphere packaging
1. Introduction
Rambutan (Nephelium lappaeum Linn.) cv. RongRien is one of Thailands economic crops with a sizeable
export market. It has been classified as nonclimacteric fruit
(OHare, 1995). The fruit will not ripen if removed from the
tree before full maturity, the state of which can be determined
principally by appearance (OHare, 1995). Rambutan shelf
*Corresponding author.
Email address: anchalee.s@psu.ac.th
58
59
Additives used,
%(w/w)
Immersion
time, min
PET/LLDPE1 (PET)
CPP/LLDPE2 (CPP)
Nylon/LLDPE3 (nylon)
Thickness
(m)
52
120
80
128.18
932.80
36.60
Weight loss with respect to initial fresh weight in minimally processed rambutan during storage was determined.
Color change was monitored using a colorimeter (Hunterlab,
Model Colorflex, USA). pH was measured according to
A.O.A.C. (2000) with a Sartorius pH meter (model PB-20,
Germany). Total soluble solids of the samples were determined using a hand refractometer (Atago, model N1, Japan).
Microbiological quality of the samples was evaluated
by taking duplicate 25 g samples from a sealed plastic bag
and homogenizing them in sterile Stomacher bags with 225
ml of sterile buffer peptone water for 1 min at 200 beats per
min. Appropriate dilutions of 0.1 ml homogenate were plated
out in triplicate on total plate count agar and incubated at
30.01.0oC for 48 h (BAM, 2001).
2.3) Statistical analyses
Solution code /
immersion time
(min)
A/2
B/5
C/5
D/3
E/2
Type of
plastic bag
Packaging condition
nylon
Air
PET nylonCPP
Air
nylon
Air
nylon
Air
nylon
5% CO2,5% O2, 90% N2
Storage temperature
(oC)
Storage time
(days)
14
14
7
10
12
60
Peeled rambutan
146.9054.36
110.1624.61
110.1631.07
128.5245.32
119.3526.58
114.7534.71
156.0541.43
156.0641.43
123.9513.12
142.2935.20
123.9341.93
105.5717.82
174.4059.78
165.2553.61
142.2950.53
169.8567.75
110.1518.05
96.4012.20
35
(a)
30
25
20
o
4 C
o
8 C
15
10
5
0
10
12
14
Days
40
35
40
(b)
30
25
20
PET
Nylon
CPP
15
10
5
0
10
12
14
Days
61
45
40
(a)
35
30
25
non-soaking
soaking
20
15
10
5
0
0
9 10 11
Days
45
40
35
(b)
30
25
non-soaking
soaking
20
15
10
5
0
0
9 10 11
Days
Figure 2. Weight loss (%) of peeled and cored rambutan with and
without soaking in solution C packed in nylon bags and
stored at 8oC (a), of peeled rambutan with and without
soaking in solution D packed in nylon bags and stored at
8oC (b). (Solution C = 0.5% citric acid + 0.5% ascorbic
acid + 2.0% sorbital + 0.2% CaCl2 + 1.5% Ca-lactate +
0.1% pectin for 5 min, Solution D = 0.5% citric acid +
0.5% ascorbic acid + 1.0% CaCl2 for 3 min)
Table 5. Quality changes of minimally processed peeled rambutan soaking in solution E1, packed in
nylon bags with and without modified atmosphere and stored at 4oC.
Treatment
(4oC)
Control
5% CO2,
5% O2 ,
90% N2
Note:
Quality changes2
Storage time
(days)
0
3
6
9
12
0
3
6
9
12
Brix
pH
L*
0.650.06d
3.830.11bc
4.920.50abc
5.900.89ab
6.950.11a
16.671.20a
15.530.23a
14.530.50a
15.600.28a
15.400.28a
5.400.40a
4.940.05c
4.990.17bc
4.730.02d
4.800.16d
47.082.16ab
45.351.19c
44.481.94c
46.770.55bc
46.320.33c
0.600.08d
2.850.88cd
5.962.72a
5.950.46ab
7.540.43a
16.101.01a
15.800.53a
15.470.14a
14.700.14a
15.200.85a
5.400.48a
5.080.02bc
5.070.19b
4.990.02c
4.960.06c
48.202.59a
46.964.15a
45.492.41bc
45.201.41c
45.252.12c
Total viable
count (CFU/g)
< 250
4.5x106
< 250
6.4x104
62
Acknowledgments
Financial support from the National Center for
Genetic Engineering and Biotechnology (BIOTEC) Thailand
is gratefully acknowledged. Kindly support the corresponding author from the Center of Excellent on Postharvest Technology Innovation Thailand for gaining experience from
fresh-cut products three-day workshop at UC Davis in
2007 contributed to this publication is also appreciated.
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