|01 |

T h e U CLA Center for Society and Genetics and A r t | S c i p r e s e n t

t h e 8 th Annual Symposium
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Outla w B i o l o g y ?
Publ i c P a r t i c i p a t i o n i n t h e Age of Big Bio
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J a n 2 9 -30, 2010 at C(n)SI-UCLA

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Citizen Science | DIY Biology | Nano Hacking | At-Home Clinical Research |

Recreational Genetics | Synthetic Biology | Open Source Science | Ars Synthetica |
Genetic Art
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Outlawbiology.net

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Outlaw Biology is funded and organized by the UCLA Center for Society and Genetics,
a center for interdisciplinary instruction and research on issues that intersect
the social and the biological, and that bring scholars together across disciplines
and approaches. Additional support from Art|Sci Center and the California Nano-
Systems Institute.
 |02 |
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Friday January 29th Saturday, January 30th

4pm-8pm 10am-3pm
Symposium, C(n)SI Auditorium Workshop, BioFaire and Exhibition,
------------------------------------------- C(n)SI Building
i n t r o d u c tion by: -------------------------------------------

------------------------------------------- Detailed schedule = page 07

Anne Andrews (Psychiatry and Biobehavioral -------------------------------------------

S c i e n c e s Senior Research Scientist, Hatos Workshop descriptions = pages 08 - 11

C e n t e r f or Neuropharmacolog y, Semel Institute -------------------------------------------

(NPI) Member, California NanoSystems Institute)

-------------------------------------------

with:

-------------------------------------------

Gaymon Bennett
( T h e B i oF ab, SynBERC , A rs-Synthetica.net,

a n d B i o s -Technika.net)

Jason Bobe
( DIY b i o . org, Personal Genome Project,

B i o w e a t h ermap.org)

Roger Brent
( F r e d H u tchison Cancer Center, Seattle)

Philip S. Lukeman
( C h e m i s t ry, Cal Poly Pomona)

Hugh Rienhoff
( M y D a u g h tersDNA .org, Berkeley)

Meredith L. Patterson
( C T O , O s ogato Inc., Belgium)

Victoria Vesna
( A r t |S c i Center, UCLA D esign M edia A rts,

L o s A n g e les)

-------------------------------------------

m o d e r a t e d by:

-------------------------------------------

Christopher Kelty
( UCLA C e nter for Society and Genetics,

L o s A n g e les)

-------------------------------------------
 |0 3|
- - - - - - - - -----------------------------------
Gaymon Bennett
T h e B i o F ab, S ynBERC , Ars- Synthetica.net,
a n d B i o s -Technika.net
-------------------------------------------
Gaymon Bennett is the Director of Human
Practices at the BioFab (UCB) and Asso-
ciate Director of the Berkeley Human
Practices Lab (SynBERC). The Human
Practices experiment concerns the rela-
tions among and between post-genomic
science, thought, and care, as well as
the challenge of inventing forms and
venues within which these relations might
be brought together and assembled. Co-
author of: Human Practices: An Experi-
ment with Synthetic Biology and Sacred
Cells? Why Christians Should Support
Stem Cell Research.
-------------------------------------------
Jason Bobe
C o - f o u n d er, DIY bio.org
D i r e c t o r , Personal Genome Project
D i r e c t o r , Bioweathermap.org
D i r e c t o r , PersonalGenomes.org
-------------------------------------------
Jason Bobe is co-founder of DIYbio.org,
an organization that aims to help make
biology a worthwhile pursuit for citi-
zen scientists and amateur biologists.
DIYbio.org is fast becoming the orga-
nizational hub for amateur biologists
worldwide. Jason is a Director of the
Personal Genome Project (PGP) based
out of George Church’s lab at Harvard
Medical School. The PGP is the most
ambitious attempt yet to elucidate how
“genes + environments = traits” in hu-
mans by amassing comprehensive datasets
of 100k participants. Jason is also
a director of the non-profit Personal-
Genomes.org, an organization that sup-
ports the thoughtful development of
personal genomics research, technology
and practices worldwide. He also directs
the massively distributed environmental
sensing project, BioWeatherMap.org.
Jason has worked as a business develop-
ment consultant for OpenWetWare.org, as
Director of Business Development at DNA
Direct, and as an independent consultant.
He focuses primarily in the area of low-
cost biotechnologies and their impacts.
-------------------------------------------
Roger Brent
Fred Hutchison C a n c e r C e n t e r
-------------------------------------------
Roger Brent is a Member in the Division
of Basic Sciences at the Hutchinson
Center in Seattle. He is known for his
contributions to the understanding of
gene regulation and to functional ge-
nomics. His work se eks to understand
how cells sense external information,
 |0 4|
represent and transmit that information
internally, and operate on it to make
decisions. Since 1987, he has helped
write Current Protocols in Molecular
Biology, a continually updated how-to-
clone-it manual. Since the late 1990s,
he and coworkers have encouraged the
democratization of biological knowledge
and capability. At the same time, they
try to think through ways this develop-
ment is impacting human events. This
work includes security issues arising
from the now-well-established and high-
ly-disseminated ability to make self-
replicating organisms including modified
bacteria and recombinant virus.
-------------------------------------------
Philip S. Lukeman
A s s i s t a nt Professor, Chemistry,
C a l P o l y Pomona
-------------------------------------------
biokleptics - nanoscale
Phil Lukeman was born in the city of
Liverpool, England. With a chemist for
a father and a series of excellent
chemistry teachers in school, the twin
demons of nature and nurture sat on his
shoulder and whispered, “Molecules”.
Through training at Leicester Univer-
sity (Undergraduate), Cambridge Uni-
versity (Ph.D. with Jeremy Sanders)
and NYU (Postdoc with Ned Seeman), he
learned to make Interesting Supramo-
lecular Assemblies. He also discovered
that he liked explaining the synthesis
and properties of these assemblies and
the implications of their manufacture.
At Cal Poly Pomona, he uses organic
chemistry on biomolecules to make nano-
technological constructs. He is most
definitely an ‘Outlaw Biologist’, as his
work shamelessly steals both lessons
and materials from biology to achieve
these ends. He hopes you’ll find his
parable of plunder propitious.
-------------------------------------------
Meredith L. Patterson
CTO Osogato, In c .
-------------------------------------------
Born in Houston, Texas to a chemical
engineer and an English teacher,
Meredith L. Patterson was taking apart
radios at the age of five, programming
at eight, and dissecting frogs at ten.
After a three-year internship in the
bioinformatics department of Integrated
DNA Technologies during her PhD research
at the University of Iowa, biohacking
seemed like an obvious hobby. She main-
tains a basement laboratory in Leuven,
Belgium, where she is working on low-
cost, off-the-shelf techniques for mo-
difying Lactobacillus acidophilus and
other lactic acid bacteria. The overall
goal of her current research is to make
it possible to use yogurt as a carrier
 |05 |
for beneficial synthetic lactobacilli
aimed at a variety of tasks, such as
detecting harmful food contaminants
(e.g., melamine) and symbiotically re-
storing the ascorbate synthesis pathway
in humans to prevent scurvy. She is
also involved with the development of
open-source, low-cost networked labora-
tory tools, including a thermocycler
and a spectrophotometer. She is active-
ly involved with the DIYbio group and
advocates strongly against any form of
restriction on citizen science.
-------------------------------------------
Dr. Hugh Rienhoff , MD
F o u n d e r , MyDaughtersDNA .org
-------------------------------------------
Hugh Rienhoff is a San Francisco Bay
area physician and entrepreneur. He is
the founder of MyDaughtersDNA.org, an
organization dedicated to greater under-
standing of genetic conditions. He is
the CEO of a privately-held biotechnol-
ogy company and a member of the board
of directors of several private com-
panies in Boston and San Francisco.
During much of the 90’s Dr. Rienhoff
was a partner directing biotechnology
investments at the venture firm New Enter-
prise Associates. He was a founding
director of such companies as Healtheon/
WebMD (HLTH) and Aurora Biosciences
(ABSC). In 1998, he founded DNA Sciences
(originally Kiva Genetics), a diagnostic
company focused on genetic discoveries
and served as its Chairman and CEO for
4 years. Dr. Rienhoff has served as
a director on the boards of many public
and private companies. Dr. Rienhoff is
an Adjunct Scientist at the Children’s
Hospital Oakland Research Institute and
is a Visiting Scholar at the University
of California at Berkeley. He trained
in mathematics, medicine, and genetics
at Harvard University, Johns Hopkins
University, and the Fred Hutchinson
Cancer Research Center in Seattle,
Washington. He received a Doctor of
Medicine degree from Johns Hopkins Uni-
versity School of Medicine and a Bach-
elor of Arts degree in Biology and Eng-
lish Literature from Williams College.
-------------------------------------------
Victoria Vesna
Professor, Depa r t m e n t o f D e s i g n | M e d i a A r t s
at the UCLA Sch o o l o f t h e A r t s .
-------------------------------------------
Victoria Vesna is a media artist and
director of the UCLA Art|Sci center and
recently became a Visiting Professor and
Director of Research at Parsons Art,
Media + Technology program. Her work
can be defined as experimental creative
research that resides between disciplines
and technologies. With her installations
she explores how communication technol-
 |0 6|

ogies affect collective behavior and how

perceptions of identity shift in rela-
tion to scientific innovation. Victoria
has exhibited her work in 20+ solo ex-
hibitions, 70+ group shows, published
20+ papers and given over 100+ invited
talks in the last decade. She is the
North American editor of AI & Society
and in 2007 published an edited volume,
Database Aesthetics: Art in the age of
Information Overflow, Minnesota Press.
In press is Context Providers: Conditions
of Meaning in Media Arts co-edited with
Margot Lovejoy and Christiane Paul.
 |0 7|
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Saturday January 30th: Workshops at a glance

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10am 10: 30 11am 11: 30 12pm 12: 30 1 pm 1: 3 0 2p m 2: 3 0 0 0

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Welcome Wagon and Strawberry DNA Extraction
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1. Bioweathermaps
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2. DNA 2. DNA
Nano Nano
Design Design
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3. SKDB 3. SKDB
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4. Paint 4. Paint
by by
microbe microbe
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5. Acinetobacter
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6. Ars 6. Ars
Synthetica Synthetica
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7. Plasmid Recovery and Visualization
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8. Syndrome
to Diagnosis
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9. DIY
Microscopy
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10. Velolab
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11. FBI
Outreach
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12. LavaAmp 12. Lava Amp
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13. HOX Project
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14. SoCal
DIY Bio Meeting
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15. Molecular
Gastronomy
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16. Poster Exhibit
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A l l w o r kshops take place in the C(n) SI B uildiing, either in th e l o b b y o r i n t h e 5 t h f l o o r

w o r k s p a c e and Art|Sci Gallery. Ask a volunteer for details.

 |08 |
Biofaire
workshops and exhibitions
-------------------------------------------
W e lcome Wagon and
S t rawberry DNA Extrac tion
-------------------------------------------
Welcome to the Outlaw Biology Biofaire
and Exhibition. At the front table we will
have strawberries and yogurt. If you
want breakfast, help yourself. But if
you want to extract DNA from your straw-
berries, we’ll have a simple protocol
and materials set out for you to do it.
O n g o i n g throughout the day in the lobby of CNSI
-------------------------------------------
1. B i oweathermaps
with Jason Bobe
h t t p://bioweathermap.org/
-------------------------------------------
The BioWeatherMap initiative is a global,
grassroots, distributed environmental
sensing effort aimed at answering some
very basic questions about the geo-
graphic and temporal distribution pat-
terns of microbial life. Utilizing the
power of high-throughput, low cost DNA
sequencing and harnessing the drive of
an enlightened public, we propose a new
collaborative research approach aimed
at generating a steady stream of environ-
mental samples from many geographic
locations to produce high quality data
for ongoing discovery and surveillance.
Our approach will provide a unique op-
portunity to engage the public in the
scientific research process while we
address fundamental questions such as
“How diverse is the microbial life
around us?” and “How do microbial com-
munities in different habitats change
over time?” and “How can advanced se-
quencing technologies best be utilized
to address issues in biodiversity, pub-
lic health, and bio surveillance?”
RSV P required a t o u t l a w b i o l o g y . n e t
Project starts a t 1 0 a m w i t h “ f l a s h s w a b s ”
occurring at Th e UCLA A r b o r e t u m a n d t h e
UCLA Hammer Mus e u m
-------------------------------------------
2. Ou tla w DN A Na n o D e s i g n
Workshop with Philip Lukeman
-------------------------------------------
Design your own DNA nanostructures using
open-source software! Nanostructures
made of DNA follow certain rules of
construction. Philip Lukeman will ex-
plain how to do it, and explain how
these designs can be synthesized using
actual DNA and visualized using an
Atomic Force Microscope.
RSV P (at outlaw b i o l o g y . n e t ) o r b r i n g y o u r
own laptop with t h i s s o f t w a r e i n s t a l l e d :
http://caDNAno. o r g
Two introductor y s e s s i o n s a t 1 1 a m a n d 1 2 : 3 0 p m
Let’s Make it a C o n t e s t : S u b m i t y o u r d e s i g n s
by February 1, 2 0 1 0 t o c k e l t y @ u c l a . e d u
along with one p a r a g r a p h d e s c r i b i n g w h a t i t
is or what it d o e s . E n t r i e s t o b e j u d g e d b y
Art|Sci and Dr. L u k e m a n w i t h t h e w i n n i n g
entry to be syn t h e s i z e d , v i s u a l i z e d a n d d i s -
played at The Ce n t e r f o r S o c i e t y a n d G e n e t i c s .
-------------------------------------------
3. Learn SKD B with B r y a n B i s h o p
and Ben L i p k o w i t z
-------------------------------------------
Software tools for open source manu-
facturing and bioengineering. Personal
manufacturing technologies such as 3D
printing and molecular nanotechnology
promise unprecedented individual free-
dom and autonomy. But how will we actu-
ally know what to do with these machines
 |09 |
once we have them? The Social Engineer-
ing Knowledge Database (SKDB) fosters
a distributed network of software, people,
designs, and data about how technology
works. Within this structure one can
share plans for automating physical build
processes, explore the possibilites of
existing tools and infrastructure, and
bring convenience to using the produc-
tive machinery that we have today.
h t t p : / / a dl.serveftp.org/dokuwiki/skdb
T w o s e s s ions: 10:30am and 1:30pm
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4. P a int-by-Microbe!
with Mac Cowell
-------------------------------------------
Paint colorful microbes - luminescent,
fluorescent, and pigmented - on do-it-
yourself solid media. With a little time
and luck, we’ll preserve the painted
results in epoxy, like microbiological
paintings in amber.
O n g o i n g with two intro sessions,
1 1 : 3 0 a m -12 and 2:30-3pm
-------------------------------------------
5. U s e of Acinetobacter
calcoaceticus strain ADP1
as a DIY
b i oengineering platform
w i t h David Metzgar , PhD
- - - - - - - - -----------------------------------
Learn how to use this exceptionally
easy protocol for genetic modification
of ADP1. Drop-in, say hi.
Ongoing
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6. Ars -Syn t h e t ica
with Gaym o n B e n n e t t
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Ars Synthetica is a web-based multi-
media forum for engaging specialists
and non-specialists in an informed,
ethical, and open dialogue on the
emerging field of synthetic biology. It
offers multiple participatory channels
for exploring questions about ethics,
security, and how cutting-edge research
in the biosciences is organized, govern-
ed, and funded. How will synthetic
biology shape and be shaped by medicine,
energy, and environmental needs? Whose
business is ethics? What are the limits
to what we can design? These are the
kinds of questions that Ars Synthetica
will pose to expert and lay communities
alike. Our goal is to actively resist
the polemics that often characterize
public discourse about new science and
technology. We will seek the fertile
grounds for discourse between the hype
of biotechnological revolution and
fears of “playing God.” The outcome
will not provide absolute or final answers,
but it will enable a diverse range of
participant responses, perspectives,
and concerns. We want to educate and
critically engage various publics about
the challenges and opportunities of
bringing new biological entities, prac-
tices, and relations into the world.
Created by Gaymon Bennett, Paul Rabinow,
Adrian Van Allen, Marlee Tichenor,
Kevin Costa.
http://ars-synt h e t i c a . n e t /
Two demonstrati o n s : 1 0 - 1 1 a m a n d 2 - 3 p m
 |10|
-------------------------------------------
7. L actobacillus Plasmid
R e covery and Visualization
w i t h Meredith L. Patterson
-------------------------------------------
Plasmids – supercoiled extrachromosomal
DNA molecules – are the workhorses of
synthetic biology, used to introduce
novel DNA sequences into bacteria. Ob-
taining appropriate plasmids can be
difficult for biohackers due to a catch-22:
E. Coli plasmids are easy to acquire,
but acquiring and maintaining E. Coli
strains can be difficult without access
to expensive refrigeration equipment.
High-quality strains of bacteria used
in the food industry, such as Lacto-
bacillus acidophilus and Lactobacillus
bulgaricus, are easy to find in the
yogurt section of any supermarket, but
plasmids for these Gram-positive bacteria
are uncommon. In this workshop, we will
use lysozyme, an enzyme in egg whites
that is needed for isolating plasmids
from Gram-positive bacteria, and a liquid-
liquid extraction technique to isolate
plasmids from an overnight culture of
lactobacilli, and then visualize our
recovered plasmids using agarose gel
electrophoresis.
RSV P r e q uired to participate
a t o u t l awbiology.net
S t a r t s a t 10am on Saturday
-------------------------------------------
8. F r om Syndrome to Diag nosis
with Hugh Rienhoff
- - - - - - - - -----------------------------------
A walk through the phases of genetic
discovery, from someone who knows the
inside story.
P r e s e n t a tion at 11:45am-12:30pm
-------------------------------------------
9. DIY Micr o sc o p y w i t h P e o pl e
All Over T h e Wo rl d
(UCLA, Bos t o n , B a n g a l o r e , A u s t r a l i a
and more)
--------------- - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Join us for a worldwide webcam hacking
event and make your own 100x USB micro-
scope for less than $10. We’ll provide
the webcams and a live internet feed
from other workshop locations across
the world, from Bangalore to Australia.
Find out more at diybio.org/ucam
Organizational m e e t i n g + w e b c a m - h a c k i n g , 1 - 2 p m
-------------------------------------------
10. Get M ovi ng w i t h t h e V elo Lab!
with Sam S t a r r
-------------------------------------------
The VeloLab is a self-sufficient, min-
iaturized biology laboratory that can
be mobilized by bicycle. In addition to
storage space for supplies and tools,
the ultralight trailer will house com-
mon lab equipment like a small laminar
flow hood for doing culture work, an
autoclave, and an incubation chamber.
An auxiliary propane generator will
provide power to the hood and to de-
vices such as a PCR machine that could
be added to the Velolab. There is al-
ternately the possibility of equipping
the trailer with solar power.
Various locatio n s t h r o u g h o u t t h e e v e n t ,
look for it!
-------------------------------------------
11. FBI Ou tr e ac h : P r o m o t i n g
Re spons ible R e se ar ch
& Career Opp o r t u n i t i e s
with Spec i al A g e n t E d w a r d Y o u
-------------------------------------------
Discuss responsible research and the
role of the FBI with Special Agent You
 |11|
and the members of the Los Angeles
field office.
O n g o i n g discussions with a presentation
a t 1 2 : 3 0 -1:15pm
-------------------------------------------
12. L ava-Amp: The Personal
T h ermal Cycler
w i t h Guido Núñez-Mujica
a n d Joseph P. Jackson III
-------------------------------------------
The LavaAmp is a portable, inexpensive,
battery-powered thermal cycler, intended
to be used by DIY biologists, people in
developing countries, bio-enthusiasts,
farmers, and others who need specific DNA
amplification at a low cost and in a por-
table way. LavaAmp is based on new
technology, and allows a quick amplifi-
cation on the go.
T w o D e m o s: 10:30-11:30am and 1-2pm
-------------------------------------------
13. T h e HOX Pro j ect
with Victoria Vesna
a n d Collaborators
- - - - - - - - -----------------------------------
This interactive project is inspired
by the properties of the Homeobox genes
which define body regions in all animals
including humans. We seek to create an
experiential space that relates the
idea that we are all interconnected and
share the same gene. The Hox Zodiac
is a work in progress and has had two
world premieres in Asia: at the Shang-
hai International Science and Art
Exhibition 2009 and at the Incheon
International Digital Art Festival in
Korea in August, 2009. The Hox Zodiac
continues to evolve. The goal is to
entice the audience with these fasci-
nating genes that are responsible for
many different variations of legs, arms
and other parts of the body exist in
the animal kingdom. The hox gene also
plays a role in many dynamic changes in
human development and continues to be
an important subject for scientific ex-
ploration in the areas of brain devel-
opment, body patterning, and variation
across species.
Ongoing in the A r t | S c i G a l l e r y S p a c e o f
the C(N )SI
-------------------------------------------
14. Southern C al i f o r n i a DIY
Bio Group M e e t i n g
with Romi e L i t t r e ll
-------------------------------------------
Get together with other southlanders to
discuss ongoing DIY Bio meetings in the
area and potential local projects.
Meeting, 2-3pm i n t h e 5 t h f l o o r w o r k s p a c e
-------------------------------------------
15. Molecula r G as t r o n o m y
with chef / r e s e a r c h e r
Richard D e l e r i n s
-------------------------------------------
Learn a basic technique in molecular
gastronomy with researcher and Cordon
Bleu-trained chef Richard Delerins.
Using syringes and sodium alginate,
create vegetarian caviar. Menu includes
optional Lactobacillus bulgaris and
Saccharomyces cerevisiae.
Lunchtime exper i m e n t s a t 1 2 - 1 p m
-------------------------------------------
16. I RE AD THE NEW S TOD A Y . . . OH B OY
A poster e x h i b i t i o n b y H e n r i L u c a s
-------------------------------------------
Since 2005, Lucas has been involved in
designing material for the UCLA Art|Sci
 |12|
-------------------------------------------

center and the UCLA Art|Global Health Thanks

center. This exhibition presents an over- -------------------------------------------

view of works by Lucas and his student organize:

assistants. He is Professor and Chair -------------------------------------------

of the Department of Design Media Arts. Ana Wevill

Roland McFarland
O n g o i n g on the 5th floor workspace Don Ponturo
Nikki Lin
Erica Stanley
Sara Scotti
Jessica Lynch-Alfaro
Mike Alfaro
diybio.org and the diybio mailing lists
-------------------------------------------

volunteer:

-------------------------------------------

Matt Abad-Santos
Rasha Ahmed
Liyna Anwar
Joan Patrice Gumasing
Jonathan Kemnitz
Talia Lester
Jessica Martinez
Rachel Mundstock
Marilyn Ponce
Marshall Louis Reaves
Jillian Theil
Jennifer Truong
Stephanie Vow
-------------------------------------------

beautify:

-------------------------------------------

Henri Lucas
-------------------------------------------

support:

-------------------------------------------

The Center for Society and Genetics

Art|Sci Center
C(n)SI