Tayyub Ali
Muhammad Fahad Khan
Kacper Rusiak
Jaleed Ahmed
YOUTUBE VIDEO
http://www.youtube.com/watch?v=78RB
pWSOl08#t=58
0.58s - 1.56s
IMPORTANCE OF OSTEOBLASTS
OSTEOBLASTS FUNCTION
APPLICATIONS
nanotubes
APPLICATIONS
DISTRIBUTORS
BONE CELLS
Mesenchymal
Cells
WNT
Signalling
Skeletal
Precursor
RUNX2
Osteoprogenitor
Preostioblast
RUNX2 &
Osterix
Transcription
Factors
Initiate OBG
Osteoblasts
Wash the
Cells
Fixation of
the Cells
Stain the
Cells
Wash the
Cells
OSTEOBLAST MINERALIZATION
Seed
Osteoblast
Grow
Osteoblast
Induce
Osteoblast
Differentiation
Culture of
Induced
Osteoblasts
FERMENTATION OF OSTEOBLASTS
Osteogenic differentiation
OSTEOBLAST DETECTION
1. Prepare solution
2. Washing Cells
3. Fixation of Cells
4. Stain the cell
5. Wash and analyze
NON-FERMENTATION
-> Use osteoblasts to derive a new product
-> Mineralization of osteoblasts
-> Osteoblasts synthesize collagen, produce calcium and
phosphate
-> hydroxyapatite mineral (Ca10[PO4]6[OH]2
-> Osteoblasts are used to create bone matrix
-> Mineral content in bone is hard to pinpoint
CANADIAN REGULATIONS
-Osteoblasts research done mostly on rat and dog osteoblast cells.
-Potential tumorigenic hazards associated with heavy metal
internalization in humans
-Testing of the bioengineered bones and cartilages as well as
osteoblasts injections still not allowed in humans.
The End