Bidirection
Replication
Rolling Circle
Replication
T4 Lytic cycle
T4 injects its DNA into the host, the DNA then circularises. The DNA is replicated
via bidirection replication, the phage hijacks and uses host machinery to
replicate its DNA and ultimately reproduce.
Unlike in who uses rolling over, T4 forms concatamers via recombination of the
copies of the genome. The recombination of the various circular copies of the
phage DNA results in a concatamer. T4 doesnt make use of COS sites to package
DNA, instead DNA is packaged into phage by the headful method. DNA attaches
to the head and is packaged into T4 until it is full, enzymes then cut the DNA
regardless of the DNA sequence unlike COS enzymes.
However, the space that the T4 head has allows for a little more than just one
complete T4 genome, as a result the terminals of the DNA are redundant.
T4 genome is 166kb
There are always 2 copies of the terminal genes. In addition each particle starts a
little later in the genome sequence, the differences in the redundant terminals
lead to circular permutation, which is all due to the packaging of more than a
genomes equivalent of DNA.
T4 rapid lysis mutant
T4 usually makes small fuzzy plaques but a mutant strain causes large plaques
which are more lethal. There have been studies in which certain T4 plaques
exhibit a mixture of morphologies thus suggesting that they probably contain 2
alleles terminal redundancy.
The event of terminal redundancy also allows the possibility a phage may be
heterozygous in the region of the genome that is diploid. This then leads to the
possibility of a variety of phenotypes being observed.
Phage statistics
T4
Genome is 166kb
Head contains 171kb of DNA
Genome is terminally redundant
Genome is circularly permuted
Genome is 48kb
Head contains 48kb
No genome redundancy
No circular permutation due to
specific cleavage at COS sites
there is only 1 copy of each gene
because one Cos site in each
genome equivalent
Phage diversity
Single Phages
X-174
The single stranded DNA after being injected into host cell uses the host
machinery which in turn leads to the synthesis of the minus
complementary strand. The resulting is a double stranded replicative form
(RF).
X-174 rolling replication the 5 strand is peeled off but not replicated,
this strand is instead bound by singly stranded DNA binding proteins. The
coating of these proteins stops DNA polymerase from binding and
replicating the DNA. The 3 end is extended and it rolls out lengthening the
5 sing strand.
The single stranded end attaches to the head and is chopped off after one
genomes equivalent of the ssDNA is packaged. The linear DNA molecule
can be circularised.