Authors:
David, James Martin Guanzon
Del Rio, Lourdes Antonina
Dela Pedra, Jewel
Dela Rosa, Clarisse
Fontanilla , Rafael
Granil, Hazel
Group 4, 2B-PH, Faculty of Pharmacy, UST
Abstract
This experiment is to allow students in our field of practice to prepare different buffer
solutions, determine the pH of the buffers and samples colorimetrically using different liquid
indicators and electro metrically using the pH meter, and to calculate the buffer capacity of the
prepared buffer solutions. We created buffers of the same kind with different pH levels by using
calculations like the Henderson-Hasselbalch equation. We checked the pH of each buffer by
using two methods; the Electrometric Determination of pH and the Colorimetric Determination
of pH.. The resulting colors for the different indicators showed that the buffer solution has a pH
that ranges from 3-8. Based on our obtained data, it was concluded that the electrometric method
of determining the pH provided a more precise reading compared to the colorimetric method of
determination.
I.
Introduction
II.
Methodology
III.
Samples
Distilled Water
Assigned Samples
(Smart C Juice)
Buffer
HPO4- 0.1
M
Secondary
Phosphate Buffer
pH
8.15
Actual
pH
7.5
[H+]
7.1 x 10
-9
0.00001
[H+]
III.16
x 10
-8
Table.1Electrometric pH Readings
Acid-Base
Indicator
2.0
3.0
7.0
7.5
8.0
12.0
Distilled
Water
Secondar
y
Phosphat
e Buffer
Yellow
Thymol
Red
Yellow
Yellow
Yellow
Yellow
Blue
Red
Bromophenol
Blue
Bromophenol
Green
Bromocresol
Purple
Phenol Red
Yellow
Yellow
Blue
Blue
Blue
Blue
Light
Yellow
Light
Yellow
Yellow
Yellow
Blue
Blue
Blue
Blue
Light
Yellow
Yellow
Violet
Violet
Violet
Violet
Light
Violet
Light
Blue
Yellow
Red
Red
Red
Red
Yellow
Methyl Red
Light
Red
Light
Red
Colorles
s
Light
Red
Red
Yellow
Yellow
Yellow
Red
Yellow
Yellow
Yellow
Orange
Orange
Colorles
s
Colorles
s
Colorles
s
Colorles
s
Yello
w
Yello
w
Red
Light
Blue
Light
Orange
Light
Orange
Yellow
Colorles
s
3-7
Colorless
Methyl Orange
Phenolphthalei
n
pH
Blue
3-8
Table.2Colorimetric pH Determination
IV.
References
[1]
http://chemwiki.ucdavis.edu/Physical_Chemistry/Acids_and_Bases/Buffers/Hende
rson-Hasselbalch_Approximation
[2]
Ferenc Szabadvry (2013-10-22). History of Analytical Chemistry: International
Series of Monographs in Analytical Chemistry. Elsevier Science[3]
[3]
http://wwwchem.csustan.edu/chem1112/1112bufferKS.htm
[4]
Malhotra (2003). Practical Biochemistry for Students. Jaypee Brothers Publishers.
[5]
http://wwwchem.csustan.edu/chem1112/1112bufferKS.htm
[6]
Dr. B. K. Sharma. Analytical Chemistry: (Comprehensively Covering the UGC
Syllabus). Krishna Prakashan Media