Wear proper attire when using autoclave safety glasses, lab coat,
closed toe shoes, and insulated gloves for protection.
Power to autoclave should be ON all the time if panel is not lit check
that POWER switch and control switch on panel are ON. If they do not
operate contact Caroline Golt in 112 Worrilow for service. Do not
attempt to use autoclave if there is a problem.
Place all of your items in an autoclavable tub with sides at least 5 high
which can contain twice the volume of liquid in case there is boilover or
breakage. Dry items should also be placed on trays or tubs.
Make sure that the plastic tub is polypropylene designed for autoclave
use.
Strat-Line autoclave tape will indicate when items have been
autoclaved.
TIME for length of cycle is set by turning the thumb wheel on the front
panel to the selected setting. Check to see that the digital display
corresponds to the time set.
Close door making sure that upper left side is pushed in as far as
possible.
Tighten door clockwise until DOOR UNLOCKED light is no longer lit. If
light does not go out after door is reasonably tight loosen door and try
again door switch may need repair.
WAIT 1 minute to make sure that no steam is escaping from door area
and that pressure is increasing (upper right of control panel).
Write your NAME and ROOM NUMBER legibly on the paper tape for your
cycle.
At end of cycle LED panel will indicate CYCLE COMPLETE wait until
pressure reading (upper right of panel) is less than 1 lb.
BEEP will sound at end of cycle. Press RESET button.
The autoclave produces steam which can burn you when the door is
opened. Stand behind autoclave door (to the left side) as you open it to
use door as a shield. Loosen door slowly counterclockwise just 1 inch
and wait for steam to be released. Open door fully to remove tray
making sure that your hands and arms are protected from burns.
Use caution when removing liquids. Sudden full opening of the door
can cause liquids to boil over or bottles to break. Let them cool slightly
in the autoclave so that bumping does not occur. Do not remove
anything from the autoclave that is still boiling. Wait until load has
cooled.
CYCLE CHOICES
Sterilization conditions differ with the type of materials to be sterilized.
GRAVITY cycle is for all dry items tips, flasks, tubes, etc. Time for
cycle will depend on volume of material. There are extra wire shelves
on the side of the autoclave so that several layers of trays may be
loaded. A dry time may be added to cycle for dry loads.
If you make a mess you are responsible for cleaning it up. If you
need help please see Caroline Golt in Room 112 Worrilow x2627 or
Brian Ladman in 315 Worrilow phone x8734.