Bio 1111, Lab 1: Schedule and list of exercises.

Spring 2015
(use this sheet to guide your reading in the lab manual)
Lab 1, Week of : January 19th.
Part 1: Introduction to the lab, administration and safety procedures.
1. Review of student guidelines and lecture on safety procedures using CST Student Guidelines manual.
You must have the Safety manual with you to sign a safety training sheet to be in the labs.
Part 2: Introduction to the Microscope; Lab 1 in Symbiosis lab manual
Review the objectives on page 1 on the lab chapter. You should be able to:
1. Identify and be knowledgeable about usage for the compound and stereomicroscopes.
2. Distinguish between prokaryotic and eukaryotic cells
3. Distinguish between the major structures of plant and animal cells
4. Recognize in cells the increasing complexity of organization and understand the evolutionary significance.
1. The 1st, 2nd & 3rd exercises introduce the student to the use of, and care for, our compound and stereo
microscopes. The goal of this part is to learn how to use the microscopes properly for good results and to avoid
breakage problems
Prepared slides used for this part are:
a.) Letter e; used to demonstrate basic scope techniques
b.) Colored threads; used to demonstrate more advanced aspects of the microscope.
2. The 4th exercise on the electron microscope is omitted.
3. The 5th exercise starts the investigation of cell structure and organization.
This exercise follows the same basic procedures learned in part 1, but has different specimens for
observations, both prepared slides and live material. This part introduces the student to the material for the next
series of labs, Bacteria, Protists and Plants. What students learn here with the prepared and live material will
help them in the following labs, particularly the protist lab covering singled cell organism.
a. Lab study A; Unicellular Organisms.
1. mixed bacterial species (bacilli, cocci, spirilla)
2. cyanobacteria example. slide of mixed cyanobacteria.
3. prepared slides of amoeba and paramecia, or mixed protists slide
b. Lab study B; Aggregate and Colonial organisms.
Prepared slides, some live material.
c. Lab study C; Multicellular organisms
Students will make wet mounts here of animal and plant tissues.
d. Lab Study D; Unknown culture.
Prepared slides and pond water used for samples.

Part 3: Introduction to Bacteria; Lab 2 in Symbiosis lab manual

The major objective for this part is to learn and practice aseptic techniques necessary for
microbiological studies.
This is part 1 of the two part Bacteriology lab. This week is set up, sampling environments and innoculatino
of nutrient agar in petri. The results will be viewed at the second lab where you see the results of the
inoculations from week 1 are examined.
1. Exercise 3: Bacteria in the environment.
Students study diversity of bacterial species using different environments and isolating bacteria from the
environments using basic microbiological techniques.
2. Exercise 4: Controlling bacterial growth using antibiotics, antiseptics and disinfectants.
Students study the effect of natural and synthetic chemicals on the growth patterns of bacteria.
Part 4: Microscope Rodeo and lab clean up; Procedures announced in lab.

Part 5: Additional exercise not in Lab manual

There is a pdf file with the material from the first lab titled Additional Exercise. It has pictures scanned
from the lab Photographic Atlas of a couple of Protists and some plant structures with both diagrams and
photomicrographs. The exercise is to come up with five criteria to describe and define a particular structure or
organism to identify it and differentiate it from a related organism.
Look at the picture of the amoeba and paramecium (use the photomicrographs of the live organism) to
practice this. You should be able to come up with five criteria or characteristics to contrast and compare the two
organisms. Repeat this with the plant structures on the final page. Make a line drawing of the animals with
sufficient detail so you can ID the organism and describe the difference between two related organisms. There
are files posted on Blackboard for help in drawing specimens.
This exercise does not have to be turned in. It is only to begin to develop the skills you will use for the
remainder of the labs this semester, how to observe specimens and record observations. Not only should you
practice your observational skills, but draw the organisms and structures as well for practice. Drawing has a
two-fold function. One is to help recognize the organism when you see them, in lab or on an exam. Two, as will
be explained in lab, your drawings become an assignment eventually turned in for points.