IN S T R UC T ION GUIDE

CULTIVATING SUCCESS
THROUGH SCIENCE
For more than 20 years, VICAM has been the global
provider of choice for next-generation food safety
technology and rapid mycotoxin test solutions.
The goal of securing the worlds food supply requires
knowledge, cooperation, and collaboration. To advance
that objective, VICAM works closely with government
regulatory agencies, international standards bodies,
major food industry laboratories, and leading research
institutions around the world. With a global scientific
and distribution presence, VICAM has access to the latest
research advances, industry expertise, and regulatory
information needed to design solutions that maximize
food safety and quality at every stage of the global food
supply chain.
As the trusted partner of the agricultural industry, VICAM
is proud to consistently deliver the superior products,
responsive service, and individualized support for your
industry-specific goals.

AflaM1-V Kit (25 Tests)..................................... 176003404

AFLA M1-V KIT CONTENTS

Afla M1-V Strip Test.......................................... 100000310
Afla M1-V Diluent Vials, 6 mL.......................... 100000308
Strip Test Pipettes............................................. 600000812
Strip Test Vials, 1.5 mL.................................... 600000813
Afla M1-V Barcode Set...................................... 715004500
Syringe Disk Filters 5 m................................. 600001331
5 mL Syringe..................................................... 600001333
Afla M1-V Instruction Guide.............................. 715004503
Vertu Afla M1-V Basic Equipment Package
with Printer, 110V..................................... 176003429
Vertu Afla M1-V Basic Equipment Package
with Printer, 220V..................................... 176003430

EQUIPMENT PACKAGE CONTENTS

Vertu Reader with Barcode Scanner,
110V/220V....................................................... 725000574
Afla M1-V Drawer for Vertu Reader................... 600001332
Vortex Genie II, 110V........................................ 600001219
Vortex Genie II, 220V....................................... 600001220
Vertu Printer, 110V/220V................................ 725000577
Strip Test Vial Rack........................................... 600001107
Digital Timer..................................................... G4036
Strip Test Vials (25/pk).................................... 600000813
Complete Mini Incubator 110V......................... 176003201
Complete Mini Incubator 220V........................ 176003202
Syringe Disk Filters, 5 m................................ 600001331
5 mL Syringe..................................................... 600001333

PR E C I S E QU A N T I TAT I V E FA S T

Dairy products such as milk, yoghurt and cheese provide essential nutrition to the
human diet. The protein, fat, vitamins and minerals found in dairy products support
healthy growth and development in children while also supporting the nutritional
needs of adults and supporting heart health, bone strength and healthy weight
maintenance. Milk is also believed to help fight cardiovascular disease, type 2
diabetes and osteoporosis while helping to meet daily requirements for human
nourishment.

Dairy feed is a critical requirement for dairy producers and has a direct impact
on milk production and quality. If grain, feed or forage containing aflatoxin B1 is
consumed by dairy cattle, is it then converted to aflatoxin M1 through normal metabolic
processes and is expressed in the milk at a rate of 1-2%. The International Agency
for Research on Cancer classifies aflatoxin M1 as a Group 2B (or probable) carcinogen.
Global regulatory limits for aflatoxin in milk and other dairy products range from
0.5 parts per billion (ppb) in the US to as low as 0.05 ppb for the EU, with even
lower limits for food intended for children. VICAM offers rapid, on-site test kits
and laboratory solutions that deliver the precise data required to ensure that dairy
products are both nutritious and safe to consume.

A F L A M 1 -V PR OC E DUR E F OR R AW MIL K
Set Up

Reading the Developed Strip

1.

Turn on the Vertu reader by pressing the

center key hard. Using the arrow keys,
move the curser to measure on the
display, and then gently press the center
key. You will then hear 3 beeps.

1.

After 12 minute incubation, insert the

developed Afla M1-V strip into the Vertu
reader drawer (test lines facing up with
logo to the right) and close drawer.

2.

2.

Calibrate the Vertu reader daily by

scanning in the bar code for the lot of
strips being used. You will hear a beep
when the bar code is accepted. Make sure
the lot number displayed on the reader is
the same lot number as the strips.

Press the center key on the reader to take

a reading.

3.

To print the result, move the cursor to

P in the upper left hand corner of the
display using the arrow keys then press
the center key.

4.

To run the next sample, move the cursor to

NT using the arrow keys and press the
center key. If you are using the same lot
of strips, you are now ready to insert the
next sample. If you are using a different
lot of strips, you will need to scan in the
new bar code.

3.

Turn on the power for mini-incubator and

pre-set temperature at 48 C. Let warm up
for about 10 min until 48 C is on
the display.

Sample Procedure
1.

Draw up 3-5 mL of cold (approximately

7 C) raw milk with syringe.

2.

Attach the filter to the end of the syringe.

Push sample through syringe filter and
collect enough milk to run the test in a
strip test vial. Not all the milk may go
through the filter.

3.

Add 250 L of filtered milk sample to

the Afla M1-V diluent vial using a strip
test pipettor* and mix well by vortexing 3
times for about 5 seconds each time.

4.

Place the Afla M1-V strip into the vial and

place vial in mini-incubator for
12 minutes.

* Strip Test Pipettor Use

1.

Squeeze the top bulb of the pipettor.

2.

Draw up enough liquid to fill the barrel of

the pipettor and have one or two drops of
liquid spill over into the lower bulb.

3.

At this point the barrel of the pipettor

should be completely full of liquid.

4.

To ensure accurate measurement, be

careful not to let any liquid drip out of the
pipettor before dispensing.

5.

Squeeze the upper bulb to dispense

the liquid.

6.

Squeeze all of the liquid out of the barrel

when dispensing.

Strip tests must be refrigerated. Store at 4 - 8 C (39 - 46 F).

Strip test should be at room temperature before use.
4

Use in accordance with VICAMs testing procedure. To be used for testing food products only.
6 Not to be ingested or applied to the skin.

Notes
1. When scanning in the bar code using the
bar code scanner, make sure the red + in the
center of the bar code scanner is in the center
of the bar code. You may need to move bar
code scanner up and down to get to the correct
position for scanning.
2. Press the center key gently and quickly.

Technical Assistance
For assistance please contact your local
distributor or Vicam Technical Services by phone
at 800-338-4381 in the United States or e-mail
to techservice@vicam.com.

BENEFITS
Results in 12 minutes*
Simple No special
training required
Precise Limits of
detection as low as 25 ppt
Convenient Easily performed
onsite or in the lab
Accurate Real-time data
which can be printed or
downloaded to a computer
Wide Range - 0 to 750 ppt
*after filtration

KEY LOCATIONS
Headquarters:
34 Maple Street
Milford, MA 01757
USA
Tel.: +1 800 338 4381
+1 508 482 4935
Fax: +1 508 482 4972

Orders:
1848 N. Deffer Drive
Nixa, MO 65714
USA
Tel.: +1 877 228 4244
+1 417 725 6588
Fax: +1 417 725 6102

www.vicam.com

Subject to change without notice.

2014 Waters Corporation. Waters, The Science of Whats Possible and VICAM are registered trademarks of Waters Corporation.
Cultivating Success Through Science and Afla M1-V are trademarks of Waters Corporation.
The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with Afla-M1 products. These analytical methods
have been validated in the VICAM laboratories to perform to the specifications indicated in the Afla M1-V procedures. The user assumes all risk in using Afla M1-V analytical procedures
and products. VICAM makes no warranty of any kind, expressed or implied, other than that Afla M1-V products conform to VICAMs printed specification and quality control
standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAMs undertaking to repair or
service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness
for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to
person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or Afla M1-V products.

715004503 June 2014 SC-PDF