ProductSheet
Bifidobacteriumlongum
subsp.longum(ATCC
15707)
Designation:E194b(Varianta)
DepositedName:BifidobacteriumlongumReuter
ProductDescription:
Typestrain.FormerlyBifidobacteriumlongum.ProducesrestrictionendonucleaseBloI.
Propagation
Medium
ATCCMedium2107:ModifiedReinforcedClostridialAgar/BrothMedium(prereduced)
ATCCMedium260:Trypticasesoyagar/brothwithdefibrinatedsheepblood
PleasereadthisFIRST
StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section
BiosafetyLevel
1
IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bifidobacteriumlongumsubsp.longum
(ATCC15707)
GrowthConditions
Temperature:37C
Atmosphere:Anaerobicgasmixture,80%N210%CO210%H2
PropagationProcedure
1. Letvialthawatroomtemperatureunderastreamofanaerobicgas.
2. Oncethaweddrawthecellsintoananaerobicsyringe(seebelow)andinoculateonetubeof#2107
broth.
3. Asepticallytransfer0.5mLofthisculturetoasecondtubeof#2107.Aslantof#2107mayalsobe
inoculatedwith0.2mLoftheculture.Streakseveralbloodplatestocheckforcolonialmorphologyand
purity.
4. Incubatetubesunderananaerobicatmosphereat37C.IncubatetheBrucellabloodagarplate
anaerobicallyforcolonyformation,andthebloodagarplateaerobicallyforaerobiccontamination
check.
5. Within48hours,growthshouldbeevidentbyturbidityandasmoothsedimentinthebroth,sedimentat
thebaseoftheslant,andcoloniesonanaerobicagarsurfaces.ColoniesonBrucellabloodagarare
circular,entire,glistening,smooth,andraised.Theaerobicplatemayhaveathinfilmintheareaof
heaviestinoculation,howeveritshouldshownoothersignsofaerobicgrowth.Bestgrowthon
anaerobicagarplatesisachievedbysubculturinganactivebrothculture.
ANAEROBICCONDITIONS:
Anaerobicconditionsfortransfermaybeobtainedbyeitherofthefollowing:
Useofananaerobicgaschamber,or
Placementoftesttubesunderagassingcannulasystemhookedtoanaerobicgas.
Anaerobicconditionsforincubationmaybeobtainedbyanyofthefollowing:
Loosescrewcapsontesttubesinanaerobicchamber,
Loosescrewcapsontesttubesinanactivatedanaerobicgaspackjar,or
Useofsterilebutylrubberstoppersontesttubessothatananaerobicgasheadspaceisretained.
Syringescanbemadeanaerobicbyoneoftwomethods:a)displacethedeadspaceinthesyringewith
asterileoxygenfreegas,orb)displacethedeadspaceinthesyringewithareducingagent.
Notes
Alwaysusefreshlypreparedprereducedmediaorprereducedmediathathasbeenpreviouslypreparedbut
storedunderanaerobicconditions.Resazurininthemediaisacolorindicatorforanaerobicconditions.
Observanceofpinkcolorinmediumbeforeuseorduringincubationshowsanaerobicconditionshavenot
beenmetandoxidationhasoccurred.Mediumshouldbediscarded.
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
References
AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
BiosafetyLevel:1
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org
Orcontactyourlocaldistributor
Page1of2
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.
ATCCWarranty
ProductSheet
Bifidobacteriumlongum
subsp.longum(ATCC
15707)
Disclaimers
PleasereadthisFIRST
StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section
BiosafetyLevel
1
IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bifidobacteriumlongumsubsp.longum
(ATCC15707)
AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org
Orcontactyourlocaldistributor
Page2of2
TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas
beenconfirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
reasonableeffortismadetoinsureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2013.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[07/31]