Short report
de Provence,
Laboratoire Dynamique et Ressources du Vegetal,
E.A. 2202-Biodiversite-Universite
UFR DENTES et SVTE, case 17, 3 place Victor Hugo, F-13331 Marseille cedex 3, France
b
Abstract
The essential oil of Artemisia annua aerial parts, consisting of camphor (44%), germacrene
D (16%), trans-pinocarveol (11%), b-selinene (9%), b-caryophyllene (9%) and artemisia
ketone (3%), was screened for its antimicrobial activity. The essential oil remarkably inhibited
the growth of tested Gram-positive bacteria Enterococcus hirae and both tested fungi. This
oil has shown an antioxidant activity equivalent to 18% of the reference compound (atocopherol).
2002 Elsevier Science B.V. All rights reserved.
Keywords: Artemisia annua; Essential oil; Antimicrobial activity; Antioxidant activity
Plant. Artemisia annua L. (Asteraceae) w1x, aerial parts collected in June 2000 on
the banks of Huveaune river (alt. 120 m), near Marseilles (France).
Identification of the species w1x is guaranteed by a confirmed botanist. A voucher
specimen is deposited in the herbarium of the University of Provence (MARS
2000.2).
*Corresponding author. Tel: q33-4-91-10-62-61; fax: q33-4-91-10-63-66.
E-mail address: vmasotti@newsup.univ-mrs.fr (V. Masotti).
0367-326X/02/$ - see front matter 2002 Elsevier Science B.V. All rights reserved.
PII: S 0 3 6 7 - 3 2 6 X 0 2 . 0 0 1 7 5 - 2
533
Table 1
Chemical composition of Artemisia annua essential oil (GC and GC-MS analysis)
Compound
RIa
1,8-cineole
artemisia ketone
artemisia alcohol
trans-pinocarveol
camphor
pinocarvone
chrysanthenol
borneol
terpinene-4-ol
myrtenal
a-terpineol
myrtenol
pinocarveyl acetate
a-copaene
benzyl isovalerate
b-cubebene
b-caryophyllene
(E)-b-farnesene
germacrene D
b-selinene
bicyclogermacrene
(E,E)-a-farnesene
germacrene A
g-cadinene
epi-a-cadinol
Total identified
1026
1058
1082
1146
1146
1161
1165
1166
1177
1192
1192
1197
1297
1379
1383
1391
1423
1457
1484
1489
1495
1503
1507
1518
1619
1,2
2,8
0,2
10,9
43,5
2,1
0,3
0,2
0,3
0,1
0,1
0,2
0,1
1,8
tr
0,1
8,9
0,1
15,6
9,4
0,3
tr
0,7
0,1
0,2
99,1
Uses in traditional medicine. Plant is known to be antimalarial w2x, anti-inflammatory w3x, antitumor w4x and allelopathic w5,6x.
Studied activity. Antibacterial and antifungal activity from essential oil by liquid
diffusion method w9x. Antioxydant activity measured by chemiluminescence w10x.
534
Table 2
Antibacterial activity of Artemisia annua essential oil
Microorganisms
Essential oil
Penicilline G
Nystatine
Escherichia coli
NT
Staphylococcus aureus
Enterococcus hirae
GIC50s0.05
CIs0.1
GIC50s0.1
CIs0.2
GIC50s0.1
CIs0.2
GIC50s0.03
CIs0.05
GIC50s3=10y4
CIs5=10y4
GIC50s3=10y4
CIs8=10y4
NT
Candida albicans
Saccharomyces cerevisiae
NT
NT
NT
GIC50s3=10y3
CIs6=10y3
GIC50s3=10y3
CIs6=10y3
Conclusions. The essential oil of Artemisia annua aerial parts remarkably inhibited
the growth of all tested fungi and Enterococcus hirae. The essential oil composition
wcamphor (44%), germacrene D (16%), trans-pinocarveol (11%), b-selinene (9%),
b-caryophyllene (9%) and artemisia ketone (3%)x and the observed antibacterial
properties show that the oil has a good potential for use in aromatherapy, despite
its weak antioxidant activity.
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