http://www.caspianapiaries.

com

Three generations of bee keeping

Over 150 years of experience in the bee industry

Over 50 by products for human consumption,

cosmetics, and animals
Commercial Royal Jelly Production and
distribution

 Biological AFB disease Control

N Chromosome Royal Jelly
A new method of commercial
venom collection

Commercial Beekeeping in
Canada, USA, and Iran
Commercial producer of
Honey Bee By-products
Genetic Programs and Queen
artificial inseminations.

N Chromosome Royal Jelly & Pheromones

has been used for AFB

controls with a 100% success rate. These same active ingredients

have been used in human health.

Bee Venom Collector device: VC-6F

Created by Michael Simics

Bee Venom Collector

Device: VC-6F

Created by Michael Simics

Venom collection is one of the most difficult

part of bee keeping process.

The following factors make it

difficult to successfully maintain
bees; even beekeepers are
unaware about the true value of
bee venom.

Creating the venom electric device

Health and size of the colony populations
Number of bee hives
Nutrition of the hives
Risk of bees attacking any livestock around the
beehives
Temperature and humidity during the collection
process
The difficulty of the large scale productions
processing venom production & collection
Marketing: producing by products by bee venom
Government regulation regarding the products.

Apitoxin, or honeybee venom, is a bitter colorless liquid. The active portion of apitoxin is a complex
mixture of proteins, which causes local inflammation and acts as an anticoagulant. The venom is
produced in the abdomen of worker bees from a mixture of acidic and basic secretions. A honeybee can
inject 0.1 mg of venom via its stinger.
Bee venom has many commercial, medicinal and therapeutic applications. Among the growing list of
uses, Bee Venom Therapy (BVT) has been used to treat arthritis, rheumatism, skin diseases, Lyme
disease and chronic fatigue syndrome.

Historically, collecting bee venom was a laborious and technical procedure, requiring skillful of
handling of each individual bee. Recently, developments in electro-stimulation of worker bees have
lead to a revolution in bee venom collection, allowing for large-scale commercial bee venom collection
operations.

Caspian Apiaries continues to build on these advances with new techniques applied the electrostimulation process developed by Mihaly Simics (Simics, 1999). The key to these new
developments is the use Caspian Solution, a proprietary mixture of pheromones and bee
pollen. In combination with electro-stimulation techniques, Caspian Apiaries is able to
maximize the yield of bee venom in three ways: First, the techniques allow Caspian Apiaries to
collect bee venom from up to 500 hives a day. Second, the use of Caspian Solution has been
shown to increase the per colony yield of venom. Finally, the use of Caspian Solution in these
techniques has also been shown to shorten the recovery period needed before a colonys venom
can be harvested again.

By stimulating bees to release Nasamov pheromone, Caspian Solution mitigates the effects of
the alarm pheromone released as a byproduct of the electro-stimulation. The pacifying effect of
Caspian Solution makes the environment around the collecting device safer, as well as allows
for the rapid re-deployment of electro-stimulation equipment to other colonies.
This presentation will include a description of the techniques, including donor hive selection,
proper parameters for the electro-stimulation, a working time-frame for efficient and scalable
collection and the hygienic, collection, transportation, desiccation and storage of bee venom.

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MATERIALS:
VENOM
COLECTORS

 12 volt batteries (6-8 amps)

4000 STRONG HIVES

OVER 200 GLASS SHEETS

ELECTRIC WIRES
BATTERY CHARGERS
ALARMS TO PREVENT INTRUDERS

PROPER CARRIERS FOR GLASS

SHEETS (designed to prevent sunlight)

MACHINERY (USED TO TRANSPORT

GLASS SHEETS)
Proper protection (bee suit / coveralls)
HYGENIC ROOMS AND FACILITIES
DISTILLED WATER
SPONGE TO CLEAN THE GLASS AND
WIRES
CASMAN SOLUTIONS AND
PHERMONES TO REDUCE ALARM
PHERMONES
LATEX
VENOM DEVICE METERS

METHODOLOGY
A new method of venom collection has been developed. We have
created a system to collect venom from 400-600 hives each day. This
amount can be increased to a maximum of 1200 hives per day.
The new method uses three Impulse generators and Collector
frames, (each device contains 1 battery, 1 generator, and 10 collector
frames. )

These devices will be placed in an area of 100 strong

beehives.

Each colony will experience between 30-40 minutes of

electric shock before we move the device to the next set of
30 hives.

Within 10 hours of work, with two

beekeepers, we are able to collect
venom from 500 hives per day

Within 2 hours, we have the ability to

collect venom from 100 hives.

These pollen patties are fed to the bees after venom collection has occurred.
This enables the bees to rapidly produce venom (1 week)

We recommend having the bee

operations close to each other
(with in 5-15 km from each
other) with source of pollen and
honey, and a strong hive
population.

During this method

of operating each
collector will placed
on top of the hives
after the inner
cover of the hives
has been removed.
This process prevents any
residue or contamination to
the wire and glass.

 . We operate the new set of 30 hives as

quickly as possible. At this time, we also
administer the Caspian solution of
pheromones to the first group to reduce
the alarm pheromones.

Before removing the venom

collectors, a new group of hives is
opened, and the battery is
temporarily disconnected from
each collector.

 After operating on 100 hives, the glass

should be changed for the new operation.
The glass sheet will be transported in a
van, to a dark, room temperature area

Minimum size of
bee population to
collect venom is a
single box
population, but we
recommend the
double box
population.

There is some
controversy regarding
the quality of the
venom collected at
night time, versus the
venom collection
during the day.

During the night,

there is more of the
bee population
available to collect
venom.

The disadvantages of
night time collections :
More difficult to operate
Temperature
(low temperature = less venom
collection)

In our practice, venom that has been

collected either day or night time will
produce the same amount venom and
color.
It is consistently an average of 1.6
gram for four double box beehives
in July and august.

We will repeat the collection process ten

days after the first round. During the
collections, when we change the
collecting t device to another group
hives we have only a 20 minute window.

LARVA PHERMONES CREAM TO

PREVENT BEE STINGS DURING
COLLECTION

To prevent bee stings on hands during the device changing process

Larva phermones cream is used.

This is over 90% effective in preventing bee stings

To reduce alarm phermones in bees,

either liquid Caspian Solution or Pollen
Patty with Phermones can be used.
This can reduce bee aggression by 90 %
and it becomes effective in less than 5
seconds.
Consumption of Caspian Solutions increase Nasanov
Phermones and Larva Phermones reduce alarm
phermones and aggressive behaviour in 2-5 seconds.