Types of culture:
1.Pure culture
- has only one species
or one strain of
microorganism
2.Mixed culture
- has two or more
species or strain of
microorganism
3. Contaminated
- unwanted microorganisms are accidentally
grown
ex. fungi in bacterial medium
4.Stock
- for research and schools
Classification of Culture
Media
A. Liquid Media
Ex. Nutrient broth (1% peptone, 0.5%NaCl
and water)
Peptone water(peptone water and 1%
meat extract)
B. Solid Media
Ex. Nutrient agar (nutrient broth and 2% agar)
2. Complex Media
chemical composition is not specifically defined
or difficult to estimate
with added ingredients
provide special nutrients
Ex. BAP, CAP
B. According to Physical
State/Consistency
1. Solid
1.5 to3% agar (seaweeds)
Colony visible growth of microorganism on solid
media
2.Liquid
No agar
evidenced by turbidity, pellicle formation and
precipitation
Peptone water, NB
3.Semi- solid
with 0.5% agar
used to demonstrate swarming growth of Proteus
and other motile organisms
motility
transport media
SIM (Sulfide Indole Motility Medium)
2.Plated
solid media in Petri dishes
MSA, EMB, Mac Conkey, SSA.
D.According to Function or
Application
1. Basal or Base
basic medium from which other culture
media are prepared
NB, NA, Trypticase Soy Agar
2. Enriched
with additional requirements or additives for
fastidious organisms to grow
blood, serum, ascitic fluid
best source of blood is sheep because only
desired organisms will produce hemolysis
horse, rabbit
BAP, CAP, LSS (Loefflers Serum Slant)
Medium
Inhibitor
Phenyl ethy
Alcohol
Agar(PEA)
selective medium
used to cultivate
Gram positive
organisms
Inhibits E. coli,
Salmonella sp.,
Shigella.
Enterobacter,
Pseudomonas
Allows Gram
positive
organisms
Staphylococcus,
Streptococcus,
Bacillus,
Clostridium
phenylethyl
alcohol,
inhibits or
reduces
growth of
Gram
negative
organisms by
interfering
with DNA
synthesis
Fermentable
Carbohydrate
Indicator
Color of Colony
Medium
Inhibitor
Vibrio parahemolyticus
Fermentable
Carbohydrate
Sucrose
Indicator
pH indicator
Thymol blue
Bromothymol
blue
H2S indicator
Sodium
thiosulfate
Ferric citrate
Vibrio cholera
Color of Colony
SFYellow
Ex. V. cholera
NSFGreen
ex. V. parahemolyticus
Medium
EMB (Eosin
Methylene Blue)
used to isolate
fecal coliforms
inhibits Gram
positive
organisms
Inhibitor
Eosin Y
Methylene
Blue
Fermentable
Carbohydrate
Lactose
Indicator
Color of
Colony
pH indicator
Eosin Y
Methylene
blue
Medium
MacConkey
selective
medium for
Gram
negative
organisms
Inhibitor
Bile Salt
Crystal
violet
Fermentable
Carbohydrate
Lactose
Indicator
pH indicator
Neutral red
(red at a pH
below 6.8
colourless at
pH greater
than 6.8)
Color of
Colony
LF- pink to red
(E.coli,
Klebsiella,
Enterobacter)
NLF
colorless
(Salmonella,
Shigella,
Proteus)
Medium
SSA
(Salmonella
Shigella Agar)
selective and
differential
media for
isolation of
pathogenic
enteric bacilli
Salmonella
and Shigella
Inhibitor
Brilliant green
Bile Salts
Citrate
Fermentable
Carbohydrate
Lactose
Indicator
Color of
Colony
Medium
MSA
(Mannitol Salt
Agar)
selective
isolation of
Staphylococci
Inhibitor
Fermentable
Carbohydrate
High
Mannitol
concentration
of salt (7.5%)
inhibits
bacteria
Indicator
Color of
Colony
Phenol
red
Medium
Bismuth
Sulfite Agar
(BSA)
isolation and
cultivation of
Salmonella
species
Salmonella
typhi
Inhibitor
Brilliant green
Fermentable
Carbohydrate
Glucose
Indicator
Bismuth
sulfite
H2S
indicator
Ferric
Sulfate
Color of
Colony
Metallic
colonies
with black
ring
Medium
Inhibitor
Hektoen
Bile salts
Enteric Agar
(HEA)
used to
isolate
species of
Salmonella,
Shigella
Fermentable
Carbohydrates
Sucrose
Lactose
Salicin
Indicator
Color of
Colony
pH
indicator
Bromothymol
blue
LF- carrot
orange/salmon
colored
colonies with
(E.coli)
H2S
indicator
Ferric
ammonium
citrate
Sodium
thiosulfate
NF- green
colonies
(Shigella)
colonies with
black center
Salmonella
typhimiurium
E. Coli
Enterococci
fecalis
Medium
Xylose Lysine
Deoxycholate
Bacteriostatic
Agent
Fermentable
Carbohydrates
Bile Salt
Xylose
Lactose
Sucrose
Indicator
pH indicator
Phenol red
H2S indicator
Ferric ammonium
citrate
Sodium
Thiosulfate
Color of
Colony
LF- yellow
colonies(E,c
oli)
NLFRed(Shigella
red colonies
with black
center(Salm
onella)
NLF yellow
(E.coli)
4. Differential Media
dyes or indicators are added to differentiate 2
groups of organisms growing together
MSA
phenol red
differentiates Staphylococcus sp. based on
Mannitol fermentation
MacConkey
neutral red
differentiates lactose from non-lactose fermenters
SSA
neutral red
differentiates Salmonella from Shigella sp.
EMB
Eosin Y and Methylene blue
differentiates E. coli from other enterics
5. Enrichment Medium
liquid medium to which certain substances are
added to enhance the growth of pathogenic
organisms and suppress the unwanted
Selenite broth
enrichment medium for Salmonella typhi
APW (Alkaline Peptone Water)
solution enrichment medium for Vibrio cholerae
6. Transport
used when there is
anticipated delay in bringing
the specimen from source
to laboratory
Cary-Blair
Vibrio sp.
Stuart medium
V. cholerae
7. Assay Media
for research
used for assay of vitamins, a. acids and antibiotics
8. Special Media
A. Special Media for Biochemical Test
1. Tryptone Broth
- identifying Gram- based on Indole
production
2. MR-VP or Peptone Glucose Broth