NAME
CLASS
: 1 HIJAU
TITLE
SCIENCE
TEACHER : MR. CHUA
Bil
Contents
Page
Acknowledgement
2. Don't view slides which are wet; wipe te slides with tissue paper.
3. Don't use the high power objective lens without covering the specimen with a cover
slip.
4. Don't squint down the microscope; view the specimen with both eyes open.
Technique in Mounting a Slide on a Microscope for Viewing.
1. Place the microscope in a well-lit place such as in front of a window.
2. Fit in the eyepiece for the microscope.
3. Fit in the low power objective lens and turn it to position.
4. Look down the eyepiece and adjust the mirror so that a bright view is obtained.
(Make sure the diaphragm is open if there is one.)
5. Place the prepared slide on the stage so that the specimen to be viewed is in the
middle of the hole on the stage.
6. Placing your eye level with the stage, slowly lower the objective lens until it is very
close
to the slide.
7. Looking down the eyepiece, turn the coarse adjustment knob towards you (raising
the
objective lens) until the specimen is in focus.
8. Change the objective lens to a medium of high power if necessary. Use the find
adjustment for the high power objective lens.
Technique in Mounting a Specimen on a Slide.
1. Place the specimen in the middle of a clean slide.
2. Place a drop of water on the specimen.
3. With the help of your fingers and a long needle, gently lower a cover slip on the
specimen.
Do not trap any air bubbles!
4. Use a piece of filter paper to wipe away any water around the cover slip. The slide is
now
ready for viewing.
5. If you wish to stain the specimen:
a) place a drop of iodine solution or ethylene blue solution at one end of the cover
slip.
b) use a strip of filter paper at the other end of the cover slip to draw the solution
across the specimen, using the irrigation technique.