Research Note
Autoxidation of corn and soybean oils was prevented in the presence of 0.1 g/kg rosemary (Rosemarinus officinalis L.) extract
and butylated hydroxy toluene (BHT). At the end of a 4-d period, the peroxide value in corn oil reached 13.0 and 8.2 in the
presence of rosemary extract and BHT, respectively. For soybean oil, the peroxide value was found to be 10.0 and 6.6 in the
presence of rosemary extract and BHT, respectively. Rosemary extract and BHT, when added as mixtures of 75:25, 50:50 and
25:75 had a synergistic effect on preventing oxidation of soybean oil. Superoxide radicals (O2) were generated by phenazine
methosulphate-NADH system and rosemary extract was found to scavenge O2 in a concentration-dependent manner. The
antioxidative effect observed is believed to be partly due to this radical scavenger activity.
Introduction
Lipid oxidation may reduce the flavor and nutritive
value of fats, oils and lipid-containing products. Unsaturated fatty acids are sensitive to oxidation because of
their chemical structure (1). Protein cross-linking,
denaturation, polypeptide chain scission, enzyme inactivation and amino acid destruction in the presence of
oxidizing lipids have been reported (2). Nutritional
quality losses are related to losses in essential fatty
acids, amino acids and vitamins (3). To overcome this
problem, synthetic antioxidants such as butylated
hydroxy toluene (BHT) and butylated hydroxy anisole
(BHA) are incorporated into fats and oils.
It has been known for some time that addition of
certain aromatic herbs or spices to lipid-containing
materials will delay the oxidation process (4, 5).
Therefore, in recent years a lot of interest has been
devoted to preparing antioxidants from natural sources
by extraction, purification and fractionation (6, 7). The
mechanism of protection given by an antioxidant was
postulated to occur at the initial stage and more
effectively during the propagation stage of oxidation by
peroxy radical (ROO ) scavengers such as phenolic
compounds (8).
Eqn [1]
0023-6438/97/010105 + 04$25.00/0/fs960127
105
POV (meq/kg)
14
12
10
8
6
4
2
0
2
Days
18
16
12
*
10
8
6
4
2
0
2
Days
106
18
16
POV (meq/kg)
14
12
10
8
6
*
*
2
0
2
Days
significantly enhanced by rosemary extract in preventing oxidation (Figs 2 and 3). Extract: BHT (25:75)
mixture reached a POV of 3.5, while BHT alone
reached a POV of 6.6 within 4 d of storage. BHT and
rosemary extract treatment had a stronger antioxidant
effect than either BHT or extract alone when total
additive concentration was the same for all treatments.
Similar effects were observed when sardine oil was
treated with RE and -tocopherol (16, 17). This was
probably due to the lipid antioxidant activity of BHT
and the ability of the extract to regenerate BHT.
Reducing equivalents supplied by rosemary extract
could have been responsible for this effect by regenerating and maintaining the antioxidative effect of
BHT.
Decomposition of hydroperoxides generates aldehydic
compounds that can be quantified by the TBA method
and this method is generally accepted as an effective
method for following lipid oxidation (18). The extract
was found to be as effective as BHT in preventing the
formation of TBA reactive substances (Table 1) and the
results are significantly different from the control
(P < 0.05). Absorbance values for the two oils studied
did not differ in the control samples and rosemary
extract was as effective as BHT when added at 0.1 g/kg
concentrations.
A common test of antioxidant ability is a compounds
effectiveness in reacting with radicals generated in
chemical model systems. For this purpose generation of
Table 1 TBA values at 532 nm of soybean and corn oils after 4 d at 30 C in the presence of antioxidants added as 0.1 g/kg.
Mean values (x) and standard deviations (sx) for n=46
Control
Sample
Soybean oil
Corn oil
RE
BHT
sx
sx
% inhibition
sx
% inhibition
1.0
1.0
0.028
0.042
0.8
0.7
0.042*
0.014*
20
30
0.6
0.5
0.014*
0.010*
40
50
*Significantly different from the control, P<0.05, one tailed student t-test.
107
A560
x
0.411
0.091
0.369
0.252
0.160
0.120
0.116
sx
0.006
0.036*
0.017*
0.040*
0.012*
0.012*
0.050*
% inhibition
77.8
10.2
38.7
61.1
70.8
71.8
Acknowledgment
This work was supported by Turkish Scientific and
Technical Research Council grant no TBGAG-95.
References
1 ADDIS, P. B. AND WARNER, G. J. The potential health
aspects of lipid oxidation products in foods. In: ARUOMA,
O. I. AND HALLIWELL, B. (Eds), Free Radical and Food
Additives. London: Taylor and Francis, pp. 77113 (1990)
2 ERIKKSON, C. F. Lipid oxidation catalysts and inhibitors in
raw materials and processed foods. Food Chemistry, 9,
311 (1982)
3 POKORNY, P. Stabilisation of fats by phenolic antioxidants.
Journal of Canadian Institute of Food Technology, 4, 6873
(1981)
4 CHIPAULT, J. R., MIZUNO, G. R., HAWKINS, J. M. AND
LUNDBERG, W. P. The antioxidant properties of natural
spices. Food Research, 17, 4651 (1952)
5 CHIPAULT, J. R., MIZUNO, G. R. AND LUNDBERG, W. P. The
antioxidant properties of spices in foods. Food Technology,
10, 209214 (1956)
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