ABSTRACT

Maruf, Fajaruddin. 2014. Antimicrobial Effect of Date Fruit Extract (Phoenix dactylifera)
toward Salmonella typhi in In Vitro Way. A Thesis, Medical Faculty of Muhammadiyah
University of Malang. Advisors: (1) M. Lutfi Manfalutfi (2) Indah Serinurani Effendi
Background of Study: Typhoid fever is one endemic disease of Indonesia. Increasing trend of
the incidence of multiple drug resistant (MDR) in the area of India and Southeastern Asia
including Indonesia increases. Date (Phoenix dactylifera) contains some chemical substances
such as flavonoid, saponin, alkaloid, and tannin as antimicrobial substances.
Research Objective: To prove the effectiveness of antimicrobial of date fruit extract (Phoenix
dactylifera) toward Salmonella typhi in in vitro way.
Research Method: A true experimental research design with post-test only control group design.
The research method was tube dilution test with a concentration of 100%-0% to determine
Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The
data analysis used ANOVA, correlation and regression.
Result: MIC and MBC were found at concentration of 25%. The result of ANOVA test showed that
there was a significant difference in the two treatments (p=0.000<0.05). The value of correlation
test was -0.839 meaning that the increasing of date fruit extract concentration decreased the
number of Salmonella typhi bacteria colony. The addition of date fruit extract (Phoenix dactylifera)
had a significant impact toward the decreasing of the number of Salmonella typhi bacteria colony in
amount of 70.3%. The antimicrobial effect was caused by the date active substance.
Conclusion : Date fruit extract (Phoenix dactylifera) had antimicrobial effect toward growth of
Salmonella typhi colony.
Keyword : Date fruit extract (Phoenix dactylifera), Salmonella typhi, Minimum Inhibitory
Concentration (MIC), Minimum Bactericidal Concentration (MBC)
NB: PAST TENSE

ABSTRACT

Maruf, Fajaruddin. 2014. Antimicrobial Effect of Date Fruit Extract (Phoenix dactylifera)
toward Salmonella typhi in In Vitro Way. A Thesis, Medical Faculty of Muhammadiyah
University of Malang. Advisors: (1) M. Lutfi Manfalutfi (2) Indah Serinurani Effendi
Background of Study: Typhoid fever is one endemic disease of Indonesia. Increasing trend of
the incidence of multiple drug resistant (MDR) in the area of India and Southeastern Asia
including Indonesia increases. Date (Phoenix dactylifera) contains some chemical substances
such as flavonoid, saponin, alkaloid, and tannin as antimicrobial substances.
Research Objective: To prove the effectiveness of antimicrobial of date fruit extract (Phoenix
dactylifera) toward Salmonella typhi in in vitro way.
Research Method: A true experimental research design with post-test only control group design.
The research method is tube dilution test with a concentration of 100%-0% to determine Minimum
Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The data
analysis uses ANOVA, correlation and regression.
Result: MIC and MBC are found at concentration of 25%. The result of ANOVA test shows that
there is a significant difference in the two treatments (p=0.000<0.05). The value of correlation test
is -0.839 meaning that the increasing of date fruit extract concentration decrease the number of
Salmonella typhi bacteria colony. The addition of date fruit extract (Phoenix dactylifera) has a
significant impact toward the decreasing of the number of Salmonella typhi bacteria colony in
amount of 70.3%. The antimicrobial effect is caused by the date active substance.
Conclusion : Date fruit extract (Phoenix dactylifera) has antimicrobial effect toward growth of
Salmonella typhi colony.
Keyword : Date fruit extract (Phoenix dactylifera), Salmonella typhi, Minimum Inhibitory
Concentration (MIC), Minimum Bactericidal Concentration (MBC)

NB: PRESENT TENSE