LYMPHATICSYSTEM&MICROBIOLOGYLAB
Thislaboratoryexerciseoffersyoutheopportunitytoexplorestructureandfunctionofthelymphaticsystem
andtheworldofmicroorganisms.
A. Lymphaticsystem
Inclass:
a. Chosetwomicroscopeslidesoflymphaticorgansortissuesprovidedtoday.
b. Viewat400Xmagnification.
c. Sketchandlabelusinglabresources.
Online:
a. Viewamicroscopeslideofalymphnode.Sketchorcopyandpasteimageintoyourlabexerciseand
labelfourstructuresatthe100Xmagnification.Usethisresource:
http://141.214.65.171/Histology/Basic%20Tissues/Epithelium%20and%20CT/027_HISTO_40X.svs/vi
ew.apml.OthersitesareonthebottomoftheSymbiosisHomePage.
b. Citeyoursource(s)(title,author,URL,dateaccessed)
Lymphaticorganortissuesketches:
B. NonspecificBodyDefenses
1. Nameandexplainthreewaystheintegumentarysystemprovidesthefirstlineofdefense.
2. DrawlinesconnectingthephrasesinColumnAwiththetermsorphrasesinColumnB.Sometermsfrom
ColumnAwillbeusedmorethanonce.
ColumnA
ColumnB
Havemicrobialactivity
Acids
Providemechanicalbarriers
Lysozyme
Entrapsmicroorganismsenteringthe
respiratorypassages
Partofthefirstlineofdefense
Mucosae
Providechemicalbarriers
Proteindigestingenzymes
Mucus
Sebum
Skin
3.
Describetheprotectiveroleofciliainthefirstlineofdefense.
4.
Phagocytosis
a. Defineandsketchtheprocessofphagocytosis.
b. Nameandsketchtwocelltypesthatperformphagocytosis.
5. Circethelostsheep(thetermthatdoesnotrelatetotheothers)ineachrow:
a. redness
pain
swelling
itching
heat
b. neutrophils
macrophages phagocytes
naturalkillercells
c. Inflammatorychemicals histamine
kinins
complement interferon
6. Listthreecomponentsoftheinflammatoryresponse.
C. SpecificBodyDefenses
Self&Nonself
a. Drawasimplesketchofanantigenandexplainhowitoperatedinimmunity.Howdoesitrelatesto
selfandnonselfmarkers?Whatcelltypesynthesizesantibodiesandinwhatorganelle?
b. Whatisthemajorhistocompatibilitycomplex?Howdoesitcontributetohomeostasis?
c. Theimmunesystemissupposedtodefendyoufromharmfulmicroorganisms.Whydoesntitalways
work?Inotherwords,whydosomegetsickanddiefrompathogens?
d. Describehowaparticularbloodantigencompositionwouldrespondtoreceivinganoncompatible
bloodtype.
D. Microbiology
1. Bacteria
Inclassinstructions:
a. Choosethreebacterialtypesfromtheonesprovidedtoday.
b. Viewwithoilimmersion(refertoMicroscopeLab).
c. Sketchacolonyofeachandlabelwithitsname.
d. Characterizeyourbacteriatypeusingthechart.
Characterizationexample:Pseudomonasaeroginosa:Agramnegativeaerobicrodshaped
bacteriumwhichdoesnotformendospores,itperformscatalase,benzidineandoxidase
reactionsanddoesnotmetabolizeglucose.
Thisdescriptioncomesfromfindingthebacterialtypeonthechart,findingthecolumnwiththe
XthenfollowingtheXup.Thedescriptionwrittenin+,,orwordstellsyouthe
characteristicsofthebacterialtype.Plusmeanitdoesorhasthatquality,minusmeansthat
actionorqualityisabsent.
Onlineinstructions:
a. Chooseatypeofbacteriathatyouareinterestedin.Youcanstarthere:
http://tinyurl.com/bmf7fjmorfindanothersiteorbookyoulike.
b. Sketchacellorcolonybelow.
c. Writeitsname.
d. NowseeifyourbacterialtypeisincludedintheBacterialClassificationChart(seelab
instructions).Youwillseethemlistedbygenus,thefirstwordinthescientificname.
e. Characterizeyourbacteriatypeusingthechart.
Characterizationexample:Pseudomonasaeroginosa:Agramnegativeaerobicrodshaped
bacteriumwhichdoesnotformendospores,itperformscatalase,benzidineandoxidase
reactionsanddoesnotmetabolizeglucose.
Thisdescriptioncomesfromfindingthebacterialtypeonthechart,findingthecolumnwiththe
XthenfollowingtheXup.Thedescriptionwrittenin+,,orwordstellsyouthe
characteristicsofthebacterialtype.Plusmeanitdoesorhasthatquality,minusmeansthat
actionorqualityisabsent.
Bacterialcharacterizationandsketches:
2. Protozoa
Inclassinstructions:
a. Chooseaprotozoanfromthoseavailabletoday.
b. Viewwithoilimmersion(refertoMicroscopeLab).
c. Sketchandlabelandstructuresyourecognizeorusingavailablelabresources.
d. Writeafewsentencesdescribingthemodeoftransmissionandthesymptomsofthedisease.
Onlineinstructions:
a. Usingthislink,http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103878/table/T1/,chooseone
protozoandiseasefromthelist.
b. Performaninternetorlibrarysearchforanimageyoucansketchorcopyintothislabexercise.
c. Citeyoursource(title,author,URL,dateaccessedorMLAformat,ifprintresource)
d. Labelthreestructures.
e. Writeafewsentencesdescribinghowthediseaseistransmitted.
Protozoansketchesanddescription:
E. ConceptMap
Drawlinesandexplanationsbetweenthesefourorgansystems.Findonewaytheimmunesystemrelatesto
eachoftheothers.
SkeletalSystem
CardiovascularSystem
ImmuneSystem
MuscularSystem