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Name________________________________________

LYMPHATICSYSTEM&MICROBIOLOGYLAB

Thislaboratoryexerciseoffersyoutheopportunitytoexplorestructureandfunctionofthelymphaticsystem
andtheworldofmicroorganisms.

A. Lymphaticsystem

Inclass:
a. Chosetwomicroscopeslidesoflymphaticorgansortissuesprovidedtoday.
b. Viewat400Xmagnification.
c. Sketchandlabelusinglabresources.

Online:

a. Viewamicroscopeslideofalymphnode.Sketchorcopyandpasteimageintoyourlabexerciseand
labelfourstructuresatthe100Xmagnification.Usethisresource:
http://141.214.65.171/Histology/Basic%20Tissues/Epithelium%20and%20CT/027_HISTO_40X.svs/vi
ew.apml.OthersitesareonthebottomoftheSymbiosisHomePage.
b. Citeyoursource(s)(title,author,URL,dateaccessed)

Lymphaticorganortissuesketches:

B. NonspecificBodyDefenses

1. Nameandexplainthreewaystheintegumentarysystemprovidesthefirstlineofdefense.

2. DrawlinesconnectingthephrasesinColumnAwiththetermsorphrasesinColumnB.Sometermsfrom
ColumnAwillbeusedmorethanonce.

ColumnA
ColumnB

Havemicrobialactivity
Acids

Providemechanicalbarriers
Lysozyme

Entrapsmicroorganismsenteringthe
respiratorypassages

Partofthefirstlineofdefense

Mucosae

Providechemicalbarriers

Proteindigestingenzymes

Mucus

Sebum

Skin

3.

Describetheprotectiveroleofciliainthefirstlineofdefense.

4.

Phagocytosis
a. Defineandsketchtheprocessofphagocytosis.

b. Nameandsketchtwocelltypesthatperformphagocytosis.

5. Circethelostsheep(thetermthatdoesnotrelatetotheothers)ineachrow:

a. redness

pain
swelling
itching
heat

b. neutrophils

macrophages phagocytes
naturalkillercells

c. Inflammatorychemicals histamine
kinins
complement interferon

6. Listthreecomponentsoftheinflammatoryresponse.

C. SpecificBodyDefenses
Self&Nonself
a. Drawasimplesketchofanantigenandexplainhowitoperatedinimmunity.Howdoesitrelatesto
selfandnonselfmarkers?Whatcelltypesynthesizesantibodiesandinwhatorganelle?

b. Whatisthemajorhistocompatibilitycomplex?Howdoesitcontributetohomeostasis?

c. Theimmunesystemissupposedtodefendyoufromharmfulmicroorganisms.Whydoesntitalways
work?Inotherwords,whydosomegetsickanddiefrompathogens?

d. Describehowaparticularbloodantigencompositionwouldrespondtoreceivinganoncompatible
bloodtype.

D. Microbiology

1. Bacteria

Inclassinstructions:

a. Choosethreebacterialtypesfromtheonesprovidedtoday.
b. Viewwithoilimmersion(refertoMicroscopeLab).
c. Sketchacolonyofeachandlabelwithitsname.
d. Characterizeyourbacteriatypeusingthechart.

Characterizationexample:Pseudomonasaeroginosa:Agramnegativeaerobicrodshaped
bacteriumwhichdoesnotformendospores,itperformscatalase,benzidineandoxidase
reactionsanddoesnotmetabolizeglucose.
Thisdescriptioncomesfromfindingthebacterialtypeonthechart,findingthecolumnwiththe
XthenfollowingtheXup.Thedescriptionwrittenin+,,orwordstellsyouthe
characteristicsofthebacterialtype.Plusmeanitdoesorhasthatquality,minusmeansthat
actionorqualityisabsent.
Onlineinstructions:

a. Chooseatypeofbacteriathatyouareinterestedin.Youcanstarthere:
http://tinyurl.com/bmf7fjmorfindanothersiteorbookyoulike.
b. Sketchacellorcolonybelow.
c. Writeitsname.
d. NowseeifyourbacterialtypeisincludedintheBacterialClassificationChart(seelab
instructions).Youwillseethemlistedbygenus,thefirstwordinthescientificname.
e. Characterizeyourbacteriatypeusingthechart.

Characterizationexample:Pseudomonasaeroginosa:Agramnegativeaerobicrodshaped
bacteriumwhichdoesnotformendospores,itperformscatalase,benzidineandoxidase
reactionsanddoesnotmetabolizeglucose.
Thisdescriptioncomesfromfindingthebacterialtypeonthechart,findingthecolumnwiththe
XthenfollowingtheXup.Thedescriptionwrittenin+,,orwordstellsyouthe
characteristicsofthebacterialtype.Plusmeanitdoesorhasthatquality,minusmeansthat
actionorqualityisabsent.

Bacterialcharacterizationandsketches:

2. Protozoa

Inclassinstructions:

a. Chooseaprotozoanfromthoseavailabletoday.
b. Viewwithoilimmersion(refertoMicroscopeLab).
c. Sketchandlabelandstructuresyourecognizeorusingavailablelabresources.
d. Writeafewsentencesdescribingthemodeoftransmissionandthesymptomsofthedisease.

Onlineinstructions:

a. Usingthislink,http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103878/table/T1/,chooseone
protozoandiseasefromthelist.
b. Performaninternetorlibrarysearchforanimageyoucansketchorcopyintothislabexercise.
c. Citeyoursource(title,author,URL,dateaccessedorMLAformat,ifprintresource)
d. Labelthreestructures.
e. Writeafewsentencesdescribinghowthediseaseistransmitted.

Protozoansketchesanddescription:

E. ConceptMap
Drawlinesandexplanationsbetweenthesefourorgansystems.Findonewaytheimmunesystemrelatesto
eachoftheothers.

SkeletalSystem

CardiovascularSystem

ImmuneSystem
MuscularSystem

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