ISSN 0976-8157
Research Article
PHARMACIE GLOBALE
INTERNATIONAL JOURNAL OF COMPREHENSIVE PHARMACY
Received: 9 June 2011; Revised: 20 July 2011; Accepted: 30 July 2011; Available online: 7 August 2011
ABSTRACT
INTRODUCTION
Statistical analysis
The results were expressed as mean SEM and were
analysed using student t test (SPSS-14) and p value <0.05
was considered significant.
Day 1
Day 21
%
reduction
Gp-I
(N. control)
15817.86
148.8019.02
---
Gp-II
(Diab. control)
265.1712.42a
25813.01a
---
Gp-III
26013.62a
185.2512.86b 28%
(Diab.+ Aloe vera)
Gp-IV
a
162.9813.26b 37%
(Diab.+Glibenclamide) 25814.8
Statistical Comparison values are expressed as Mean SD. (n=6)
a; p<0.01 as compared to their corr. Levels in Group I.
b; P<0.01 as compare to their corr. Levels on Day 1
Sample collection
Blood samples were collected from the marginal vein of
Table 2. Showing values of HbA1c and change in body
pinna of overnight fasted rabbits. Fasting blood glucose
weights (kg) in various groups.
(FBG) was estimated before the treatment (day-1) and
Group& treatment
HbA1c%
Initial weight Final weight
after treatment (day-21). Glycosylated Hemoglobin HbA1c
Gp-I (N. control)
3.10 0.1
1.890.04
1.950.05
and Lipid profile parameters were determined at the end
Gp-II (Diab. control) 6,5 0.15
1.680.03+
1.520.04+
of the study. Initial and final body weight of all rabbits was
Gp-III (Diab. + AV)
5.25 0.18
1.630.05+
1.750.05++
recorded. The serum was separated immediately and
Gp-IV (Diab. +
5.9 0.21
1.640.07+
1.860.06++
assayed for the following parameters.
Glibenclamide)
1) Blood Glucose: By Glucose oxidase peroxidase
+ p<0.01 as compared to normal control (Group-I).
method.11
++ p<0.01 as compared to diabetes (Group-II).
2) HbA1c assay was done on auto analyzer using kits by
The levels of TG, TC and LDL-C were significantly
Randox.12
increased, where as HDL-C was significantly decreased in
3) Total Cholesterol (TC) and HDL-C and Triglycerides
diabetic rabbits (Group II), compared with the normal
(TG) were estimated on auto analyzer (Kone Lab 30i,
(Group I) (Table 3). Supplementation with Aloe vera
Trivitron) by enzymatic methods using kits by
extract led to significant reversal of these changes in
Randox.13 The concentrations of low density
diabetic animals. In Glibenclamide treated rabbits the
lipoprotein cholesterol (LDL-C) and very low density
effect was comparable to Aloe vera treated animals but
lipoprotein cholesterol (VLDL-C) were calculated as
HDL-C was not significantly increased by Glibenclamide.
described by the formula of Friedewald.14 The
Table 3. Effect of Aloe vera on lipid profile of diabetic rabbits.
Group& treatment
Gp-I (N. control)
Gp-II(Diab. control)
Gp-III(Diab.+ AV)
Gp-V(Diab.+ glibenclamide)
* p<0.05 (significant)
TG (mg/dl)
91.624.7
1104.5*
1044.3*
953.8*
S. Cholesterol (mg/dl)
60.235.9
754.86*
68.164.34*
63.484.64*
HDL-C (mg/dl)
21.222.16
15.931.8*
18.54 1.38*
16.181.26*
LDL-C (mg/dl)
20.261.66
24.181.3*
19.241.6*
18.941.6*
Atherogenic index
2.85
5.0
3.77
Protection rate %
43%
0%
26.6%
3.9
38.2%
REFERENCES
CONCLUSION