College of Life Sciences, Qingdao University, Qingdao 266071, PR China; 2Yellow Sea
Experimental
Materials
Porphyra haitanensis sample collected from the Jinjiang coastline (Fujian, China) in February
2013 was authenticated by Institute of Oceanology, Chinese Academy of Sciences.
The voucher specimen (QD-SW-20130068) was deposited in the Biochemistry Lab of
Qingdao University, Qingdao, China. 1, 8-dihydroxy- anthraquinone (Dan) was
isolated from the sample by the reference method (Feng et al., 2013). Staphylococcus
aureus (CAU0519) was supplied by Institute of Microbiology, Chinese Academy of
Sciences. Bacterial cells were cultured and activated in fresh tryptone soybean broth
(TSB) at 28 for 12 h.
Effect of Dan on the Growth Curve of S. aureus
The experiment was carried out according to reference (Ai et al. 2007) with some
modifications. Briefly S. aureus culture was inoculated in nutrient broth medium with an
inoculation amount of 106 CFU/mL and cultured at 37 with the air bath and homothermal
vibrator at 150 rpm. Optical density (OD) at 550 nm was monitored with spectrophotometer
every hour during a course of 13 hours as control group. The procedure for experimental
group was the same as above except that bacterial suspension contained 80 g/mL Dan.
mg/mL CCl3COOH and 0.6 mg/mL FeSO4) and layed up for 10 min. After each
centrifugation, the OD value of the supernatant was determined by ammonium molybdate
spectrophotometry at 630 nm as control group. The procedure for experimental group was the
same as above except that bacterial suspension contained 80 g/mL of Dan.
A-3 h
A-7 h
A-5 h
B-3 h
B-5 h
B-7 h
Fig.S7 The morphology of S. aureus after incubation with Dan for 3, 5 and 7 h
A-3 h
A-5 h
A-7 h
B-3 h
B-5 h
B-7 h
Fig.S9 The ultrastructure of S. aureus after incubation with Dan for 3, 5 and 7 h
Statistical analysis
All data were analysed by ANOVA method and expressed as mean standard deviation.
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