ALKALINE PHOSPHATASE (ALP)

Continuous-spectrophotometric
DEA BUFFER

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the

phosphate group from 4-nitrophenylphosphate to diethanolamine (DEA),
liberating 4-nitrophenol. The catalytic concentration is determined from the rate
of 4-nitrophenol formation, measured at 405 nm.

Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the

Reagent A bottle (if 10x20 mL size) or dissolve the contents of a Reagent B
vial with the entire volume of a Reagent A bottle (if 5x100 mL size).
Stable for 2 months at 2-8C.

Samples

Performance characteristics

Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8C.
Heparin may be used as anticoagulant

Linearity: up to 690 U/L.

Interferences: Fluoride, oxalate, citrate and EDTA as anticoagulants
interfere. Hemolysis interferes due to the alkaline phosphatase content in
red cells.

Instrument settings

Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

Fosfatasa alcalina
FAL
Cintica 10 %
51 seg.
405 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
0.000 Abs.
0.600 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

2764.00
90 U/l
280 U/l
90
U/l
280 U/l
-

37 C

ALANINE AMINOTRANSFERASE (ALT)

Continuous-spectrophotometric
IFCC

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino Working Reagent: Pour the contents of the Reagent B into the Reagent A
group from alanine to 2-oxoglutarate, forming pyruvate and glutamate. The bottle. Mix gently.
catalytic concentration is determined from the rate of decrease of NADH, Stable for 2 months at 2-8C.
measured at 340 nm, by means of the lactate dehydrogenase (LDH) coupled
reaction.

Samples

Performance characteristics

Serum.
Alanine aminotransferase in serum is stable for 7 days at 2-8C.

Interferences: High pyruvate in the sample will consume NADH during the
delay time before measurements, reducing the linearity of the method.
Linearity: Up to 500 U/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

ALT/GPT
ALT
Cintica 10 %
70 seg.
340 nm
U/l
0
23
ul
5
ul
230 ul
248 ul
0.600 Abs.
3.000 Abs.
3.000 Abs.
1.000 Abs.
3.000 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

-1746.0
2 U/l
41 U/l
2 U/l
41 U/l
-

37 C

-AMYLASE

Continuous-spectrophotometric
DIRECT SUBSTRATE

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Samples

Performance characteristics

Serum, plasma, urine.

-Amylase in serum, plasma or urine is stable for 5 days at 2-8C.
Heparin may be used as anticoagulant

-Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-maltotrioside Reagent is ready to be used.

(CNP-G3) to 2-chloro-4-nitrophenol (CNP). The catalytic concentration is
determined from the rate of 2-chloro-4-nitrophenol formation, measured at 405
nm.

Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).

Interferences: Fluoride, oxalate, citrate and EDTA as anticoagulants
interfere.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

AMILASA
AMY
Cintica 10 %
32 seg.
405 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
-0.100 Abs.
0.500 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

3292.00
0
U/l
60 U/l
0
U/l
60 U/l
-

37 C

CALCIUM
Spectrophotometric
METHYLTHYMOL BLUE

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Calcium in the sample reacts with methylthymol blue in alkaline medium Working Reagent: Mix equal volumes of Reagent A and Reagent B. Mix
forming a coloured complex that can be measured by spectrophotometry. thoroughly.
Hydroxyquinoline is included in the reagent to remove magnesium interference. Stable for 2 days at 2-8C.

Samples

Performance characteristics

Serum, heparinized plasma, urine.

Calcium in serum or plasma is stable for 10 days at 2-8 C.
Anticoagulants other than heparin should not be used.

Linearity: Up to 15 mg/dL.
Interferences: Hemoglobin (1.5 g/L), bilirubin (20 mg/dL), magnesium (10
mg/dL) and phosphate (20 mg/dL) do not interfere.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

CALCIO
CAL
Punto final
11.5 min
620 nm
mg/dl
1
3
ul
2
ul
300 ul
301 ul
4.000 mg/dl.
15.000 mg/dl.
-0.100 Abs.
0.600 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin

CALCIO
3
1
*
*
*
**
9.0 mg/dl
10.7 mg/dl
9.0 mg/dl
10.7 mg/dl
-

37 C

CHOLESTEROL
Enzymatic-spectrophotometric
CHOLESTEROL OXIDASE/PEROXIDASE

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Free and esterified cholesterol in the sample originates, by means of some Reagent is ready to be used
coupled reactions, a coloured complex that can be measured by
spectrophotometry.

Samples

Performance characteristics

Serum or plasma.
Stable for 7 days at 2-8C.
Heparin, EDTA, oxalate and fluoride may be used as anticoagulants.

Interferences: Hemoglobin (3 g/L), ascorbic acid (0.3 mmol/L) and

bilirubin (0.25 mmol/L) interfere. Lipemia does not affect results.
Linearity: Up to 1000 mg/dL.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. Infer.
Conc. Super.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

COLESTEROL
COL
Punto final
11.5 min
505 nm
mg/dl
0
3
ul
2
ul
250 ul
251 ul
0.000 mg/dl.
500.00 mg/dl.
-0.100 Abs.
0.300 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

COLESTEROL
3
1
*
*
*
**
123 mg/dl
270 mg/dl
123 mg/dl
243 mg/dl
-

37 C

CREATINE KINASE (CK)

Continuous-spectrophotometric
IFCC

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Creatine kinase (CK) catalyzes the phosphorylation of ADP, in the presence of Working Reagent: Empty the contents of a Reagent B vial into a Reagent A
creatine phosphate, to form ATP and creatine. The catalytic concentration is bottle. Swirl gently.
determined from the rate of NADPH formation, measured at 340 nm, by means Stable for 15 days at 2-8C.
of the hexokinase (HK) and glucose-6-phosphate coupled reactions.

Samples

Performance characteristics

Serum.
Creatine kinase in serum is stable for 7 days at 2-8C.

Linearity: up to 900 U/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

CK-NAC
CK
Cintica 10 %
129 seg.
340 nm
U/l
0
10
ul
2
ul
250 ul
258 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
-0.100 Abs.
0.800 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

4127.00
24 U/l
195 U/l
24 U/l
170 U/l
-

37 C

CREATININE
Kinetic-spectrophotometric
ALKALINE PICRATE

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Creatinine in the sample reacts with picrate in alkaline medium forming a Working Reagent: Mix equal volumes of Reagent A and Reagent B. Mix
coloured complex. The complex formation rate is measured in a short period to thoroughly.
avoid interferences.
Stable for 2 months at 2-8C.

Samples

Performance characteristics

Serum, plasma, urine.

Creatinine in serum or plasma is stable for 24 hours at 2-8C.
Heparin, EDTA, oxalate and fluoride may be used as anticoagulants.

Interferences: Hemoglobin (0.1 g/L), bilirubin (10 mg/dL), protein and

ketonic bodies do not interfere.
Linearity: Up to 20 mg/dL (serum or plasma).

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
1er punto
2do punto
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Desv. Abs. React
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

CREATININA
CRE
Dos puntos
32 seg
90 seg
505 nm
mg/dl
2
25
ul
5
ul
250 ul
270 ul
0.300 mg/dl.
20.000 mg/dl.
3.000 Abs
-0.100 Abs.
0.500 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin

CREATININA
3
1
*
*
*
**
0.60 mg/dl
1.10 mg/dl
0.60 mg/dl
1.10 mg/dl
-

37 C

PHOSPHORUS
Spectrophotometric
PHOSPHOMOLYBDATE/UV

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Inorganic phosphorus in the sample reacts with molybdate in acid medium Working Reagent: Mix 35 mL Reagent A + 15 mL Reagent B. Mix thoroughly.
forming a phosphomolybdate complex that can be measured by Stable for 12 months at 15-30C.
spectrophotometry.

Samples

Performance characteristics

Serum, plasma, urine.

Phosphorus in serum or plasma is stable for 7 days at 2-8C. EDTA and fluoride may be used as anticoagulants.

Interferences: Do not use hemolyzed sera.

Linearity: Up to 20 mg/dL.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. Infer.
Conc. Super.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

FSFORO
FOS
Punto final
11.5 min
340 nm
mg/dl
1
3
ul
2
ul
300 ul
301 ul
0.500 mg/dl.
20.000 mg/dl.
-0.100 Abs.
0.500 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin

FOSFORO
3
1
*
*
*
**
2.7 mg/dl
4.5 mg/dl
2.7 mg/dl
4.5 mg/dl
-

37 C

FRUCTOSAMINE
Kinetic-spectrophotometric
NBT

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Serum glycated proteins reduce nitroblue tetrazolium (NBT) salts in alakaline Reagent is ready to be used.
medium. The rate of formazan at a given temperature is proportional to the
serum glycated proteins

Samples

Performance characteristics

Serum.
Stable for 1 week at 2-8C and for 2 months at 20C.
Hemolysed samples are not suitable for testing.

Interferences: Hemoglobin (up to 100 mg/dL), bilirubin (up to 2 mg/dL),

uric acid (up to 15 mg/dL) and lipidemia do not interfere with the test.
Linearity: Up to 7 mmol/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
1er punto
2do punto
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Desv. Abs. React
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

FRUCTOSAMINA
FRUC
Dos Puntos
285 seg
422 seg
546 nm
mol/l
0
30 ul
15 ul
250 ul
265 ul
0.000 mol/l
7.000 mol/l
3.000 Abs
-0.100 Abs
0.800 Abs
SI (-0.000)
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin

FRUCT BIO
3
1
*
*
*
**
0 mol/l
285 mol/l
0 mol/l
285 mol/l
0
0

37 C

GAMMA-GLUTAMYLTRANSFERASE (-GT)

Continuous-spectrophotometric
IFCC

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Samples

Performance characteristics

Serum.
Gamma-glutamyltransferase in serum is stable for 5 days at 2-8C.

Gamma-glutamyltransferase (-GT) catalyzes the transfer of the -glutamyl Working Reagent: Reconstitute the contents of a Reagent B vial with 15 mL (if
group from -glutamyl-3-carboxy-4-nitroanilide to glycylglycine, liberating 3- 10x15 mL size) or 50 mL (if 4x50 mL size) of Reagent A. Swirl gently.
carboxy-4-nitroaniline. The catalytic concentration is determined from the rate Stable for 2 months at 2-8C.
of 3-carboxy-4-nitroaniline formation.
Linearity: up to 300 U/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

GGTP
GGT
Cintica 10 %
32 seg.
405 nm
U/l
0
25
ul
5
ul
250 ul
270 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
-0.100 Abs.
1.000 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

1111.00
15 U/l
60 U/l
10 U/l
40 U/l
-

37 C

GLUCOSE
Enzymatic-spectrophotometric
GLUCOSE OXIDASE/PEROXIDASE

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Glucose in the sample originates, by means of some coupled reactions, a Reagent is ready to be used
coloured complex that can be measured by spectrophotometry.

Samples

Performance characteristics

Serum or plasma.
Stable for 7 days at 2-8C.
Heparin, EDTA, oxalate and fluoride may be used as anticoagulants.

Interferences: Hemoglobin (0.3 g/L), ascorbic acid (10 mg/dL) and

bilirubin (15 mg/dL) interfere. Moderate lipemia does not affect the
results.
Linearity: Up to 500 mg/dL.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra

GLUCOSA
GLU
Punto final
11.5 min
505 nm
mg/dl
0

Vol. muest. rerun

Vol. reactivo
Vol. react. rerun
Conc. Infer.
Conc. Super.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

2 L
300 L
301 L
10.000 mg/dl.
500.000 mg/dl.
-0.100 Abs.
0.300 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.

*
*
*
*
*
*
**
70 mg/dl

Ref. Hombre sup.

Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

110 mg/dl
70 mg/dl
110 mg/dl

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin.

37 C

ASPARTATE AMINOTRANSFERASE (AST)

Continuous-spectrophotometric
IFCC

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino Working Reagent: Pour the contents of the Reagent B into the Reagent A
group from aspartate to 2-oxoglutarate, forming oxalacetate and glutamate. bottle. Mix gently.
The catalytic concentration is determined from the rate of decrease of NADH, Stable for 2 months at 2-8C.
measured at 340 nm, by means of the malate dehydrogenase (MDH) coupled
reaction.

Samples

Performance characteristics

Serum.
Aspartate aminotransferase in serum is stable for 7 days at 2-8C.

Interferences: High pyruvate in the sample will consume NADH during the
delay time before measurements, reducing the linearity of the method.
Linearity: Up to 500 U/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

AST/GOT
AST
Cintica 10 %
70 seg.
340 nm
U/l
0
23
ul
5
ul
230 ul
248 ul
0.600 Abs.
3.000 Abs.
3.000 Abs.
1.000 Abs.
3.000 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

-1746.0
2 U/l
42 U/l
2 U/l
42 U/l
-

37 C

IRON
Spectrophotometric
FERROZINE

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Transferrin-bound ferric ions in the sample are released by guanidinium and Working Reagent: Transfer the contents of one Reagent B vial into a Reagent
reduced to ferrous by means of hydroxylamine. Ferrous ions react with A bottle. Mix thoroughly.
ferrozine forming a coloured complex that can be measured by Stable for 6 months at 2-8C.
spectrophotometry.

Samples

Performance characteristics

Serum or heparinized plasma.

Stable for 7 days at 2-8C.

Interferences: Do not use hemolyzed sera.

Linearity: Up to 1000 g/dL.

Instrument settings

Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Absorb. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

HIERRO
FE
Punto final
11.5 min
578 nm
ug/dl
0
30
ul
15
ul
250 ul
265 ul
0.000 ug/dl.
1.000 ug/dl.
-0.100 Abs.
0.300 Abs.
Si
SI

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin.

HIERRO
3
1
*
*
*
**
70 U/l
155 U/l
55 U/l
140 U/l
-

37 C

LACTATE DEHYDROGENASE (LD/LDH)

Continuous-spectrophotometric
PYRUVATE

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Lactate dehydrognase (LD or LDH) catalyzes the reduction of pyruvate by Working Reagent: Reconstitute the contents of a Reagent B vial with 3 mL (if
NADH, to form lactate and NAD+. The catalytic concentration is determined 20x3 mL size) or 15 mL (if 10x15 mL size)of Reagent A. Swirl gently.
from the rate of decrease of NADH, measured at 340 nm.
Stable for 2 months at 2-8C.

Samples

Performance characteristics

Serum or plasma.
Lactate dehydrogenase in serum or plasma is stable for 24 hours at 2-8C.
Heparin may be used as anticoagulant.

Interferences: Hemolysis interferes due

dehydrogenase concentration in red cells.
Linearity: Up to 1500 U/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

LDH
LDH
Cintica 10 %
32 seg
340 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.400 Abs
3.000 Abs.
3.000 Abs.
1.000 Abs.
2.000 Abs.
Si
No

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
0
TEMPERATURA 37 C

-8095.00
207 U/l
414 U/l
207 U/l
414 U/l
-

37 C

to

the

high

lactate

MAGNESIUM
Spectrophotometric
CALMAGITE

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Magnesium in the sample reacts with calmagite in alkaline medium forming a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry. EGTA is
included in the reagent to remove calcium interference.

Samples

Performance characteristics

Serum, heparinized plasma.

Magnesium in serum or plasma is stable for 10 days at 2-8C.
Anticoagulants other than heparin should not be used.

Interferences: Hemoglobin (1.5 g/L), calcium (20 mg/dL) and bilirubin (20
mg/dL) do not interfere.
Linearity: Up to 4 mg/dL.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

MAGNESIO
MAG
Punto final
11.5 min
546 nm
mg/dl
1
3
ul
2
ul
300 ul
301 ul
0.500 mg/dl.
4.000 mg/dl.
-0.100 Abs.
0.900 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin

MAGNESIO
3
1
*
*
*
**
1.8 mg/dl
2.1 mg/dl
1.8 mg/dl
2.1 mg/dl
-

37 C

PROTEIN
Spectrophotometric
BIURET

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Protein in the sample reacts with copper (II) ion in alkaline medium forming a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry.

Samples

Performance characteristics

Serum, heparinized plasma.

Stable for 8 days at 2-8C.
Anticoagulants other than heparin should not be used.

Interferences: Hemoglobin (0.2 g/L) and bilirubin (15 mg/dL) interfere.

Moderate lipemia does not affect the results.
Linearity: Up to 150 g/L.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. Rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

PROTEINAS
PRO
Punto Final
11.5 min
546 nm
g/dl
1
5 L
3 L
250 L
252 L
0.000 g/dl
10.000 g/dl
-0.100 Abs
0.500 Abs
SI
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin

*
*
*
*
*
*
**
6.0 g/dl
8.0 g/dl
6.0 g/dl
8.0 g/dl

37 C

UREA/BUN
Enzymatic-spectrophotometric
ULTRAVIOLET

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Urea in the sample consumes, by means of some coupled reactions, NADH Working Reagent: Transfer the contents of one Reagent B vial into a Reagent
that can be measured by spectrophotometry.
A bottle. Mix thoroughly.
Stable for 2 months at 2-8C.

Samples

Performance characteristics

Serum, plasma, urine.

Stable for 7 days at 2-8C.
Heparin is recommended as anticoagulant.

Interferences: Ammonium salts of the anticoagulants interfere.

Linearity: Up to 300 mg/dL.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
1er punto
2o punto
Long. Onda
Unidades
Decimales
Vol. Muestra
Vol. Muest. rerun
Vol. Reactivo
Vol. React. rerun
Conc. Infer.
Conc. Super.
Desv.abs.react.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra

UREA
URE
Dos puntos
51 seg
110 seg
340 nm
mg/dl
0
3
ul
2
ul
399 ul
400 ul
5.000 mg/dl.
250.00 mg/dl.
3.000 Abs
1.250 Abs.
3.000 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin.

UREA
3
1
*
*
*
**
10 mg/dl
50 mg/dl
10 mg/dl
50 mg/dl
-

37 C

URIC ACID
Enzymatic-spectrophotometric
URICASE/PEROXIDASE

Instrument:
Principle of the method

SELECTRA I
Reagent preparation

Uric acid in the sample originates, by means of some coupled reactions, a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry.

Samples

Performance characteristics

Serum, heparinized plasma.

Magnesium in serum or plasma is stable for 10 days at 2-8C.
Anticoagulants other than heparin should not be used.

Interferences: Hemoglobin (1 g/L), ascorbic acid (0.3 mmol/L) and

bilirubin (15 mg/dL) do not interfere. Lipemia may affect the results
Linearity: Up to 25 mg/dL.

Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra

ACIDO URICO
URI
Punto final
11.5 min
546 nm
mg/dl
1
5
ul
3
ul
250 ul
252 ul
0.000 mg/dl.
25.000 mg/dl.
-0.100 Abs.
0.300 Abs.
Si
NO

Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

*A introducir por el operador.

**Se introduce automticamente luego de la primer calibracin.

AC.URICO
3
1
*
*
*
**
3.4 mg/dl
7.0 mg/dl
2.4 mg/dl
5.7 mg/dl
-

37 C