9.
SAICAR
Adenylosuccinase
10.
AICAR
Formyl transferase v
11.
FAICAR
IMP DH (inhib by
mycophenolic acid,
tiazofurin)
carboxamide
ribosyl-5-phosphate
(SAICAR, IX)
Aminoimidazole
carboxamide ribosyl
5-phosphate
(AICAR, X)
Formimidoimidazole
carboxamide ribsyl
5-phosphate
(FAICAR, XI)
Inosine
monophosphate
1 HEB
C atoms of Asp are released as fumarate
N1 from Asp joins with the imidazole ring
Final purine C2 removed
N10-formyl FH4FH4
Inhibited by folic acid analogs (methotrexate: inhibits reduction
of FH2FH4, trimethoprim: selectively inhibits dihydrofolate
reductase)
2nd ring closure
H2O removed from formyl, carboxamide grp
IMP rapidly converted to AMP & GMP
Joins base hypoxathine joined by a glycosidic bond N9-C1
5-PAMP)
Adenylosuccinate
Adenosine monophosphate
(AMP)
5-PGMP)
Xanthine monophosphate
(NAD dependent) (XMP)
Guanosine monophosphate
(GMP)
ATP + AMP
ATP + GMP
ATP + G/CDP
Adenylate kinase
Guanylate kinase
Nucleoside diphosphate kinase
Purine bases
(xanthine,
Hypoxanthine-guanine
phosphoribosyl transferase (HGPRT)
2 ADP
GDP + ADP
GTP or CTP +
ADP
IMP, GMP
guanine)
Adenine
Adenosine +
ATP
Adenosine deaminase
Guanine
Hypoxanthine
Guanase/guanine deaminase
Xanthine oxidase (inhib by
allopurinoloxypurinol)
Xanthine oxidase
Xanthine
AMP
AMP
IMP
Adenosine/guanosine/inosin
e
Inosine
Xanthine
Xanthine
Uric acid
Carbomoyl aspartic
acid (CAA)
3. CAA
Dihydroorolase
Dihydroorotic acid
(DHOA)
ATPADP
NH3 (ammonia) from Glu side
chain
ATP
*********REGULARE STEP
CO2 provides C2
Glu N3
2 HEB
-amino Asp condenses donates
C4, C5, C6, N1
*******COMMITTED STEP
no E input
Closed hydrolytically
H2oO released btw middle amide
grp of carbomoyl & -carboxyl
Asp
4. DHOA
6. OMP
7. UMP
Uridine
monophosphate
(UMP) (RNA
precursor)
Uridine diphosphate
(UDP)
Uridine triphosphate
(UTP)
Cytidine
triphosphate (CTP)
5. OA
8. UDP
9. UTP
10. UDP
11. dUDP
12. dUMP
Orotidine 5monophosphate
(OMP)
Deoxyuridine
diphosphate (dUDP)
(can be
phosphorylated to
triphosphate to
make dUTPs for
DNA synth)
dUMP
dTMP
Dephosphorylation
dUMP methylated @C5
methyl grp source: N5-N10methylene FH4 (1C grp transfer)
13. dTMP
14. dTDP
dTDP
dTTP (precursor dor
DNA synth,
regulates
ribonucleotide
reductase)
3b. -aminoisobutyrate
(BAIB) (from thymine)
NAD Synthesis
1. Nicotinamide (derive of
niacin-Vit B3)
Transamincase (+activation)
Nicotinamide phsophoribosyl
transferase
2. nicotinamide
NAD-pyrophosphorylase
mononucleotide
3. NAD
Phosphorylating enz
FMN/FAD Synthesis (for reversible redox rxns)
1. Riboflavin (from Vit B2)
Riboflavin kinase
2. FMN
FAD-pyrophosphorylase
A/carnosine
Methylmalonyl CoAsuccinyl
CoA to Krebs
Nicotinamide mononucleotide Nicotinamide condenses
with PRPP (ribose 5-P
donor)
PPi released
Nicotinamide adenine
P of ATP of nicotinamide
dinucleotide (NAD)
mononucleotide
NADP
P by ATP
Riboflavin phosphate (flavin
mononucleotide, FMN)
Flavin adenine dinucleotide
(FAD)
ATP
4-phosphopanthothenate
4phosphopanthenoylcysteine
ATP
ATP
4-phosphopantotheine
decarboxylation
Dephosphoconezyme A
CoEnz A
ATP