Electrochemical Studies on Interaction between ApoCopCBSA and

Cu()Cu()Cd()Ag()
Author :ZhangHui
Tutor:TianYanNi
School :Shanxi University
CLC :O646
TYPE :Masters thesis
Download the PDF Full Text:http://www.topresearch.org/showinfo-91-450973-0.html
Year:2011
Abstract:
Copper is an essential micronutrient in all living organisms. However, its excess present is toxic.
CopC is a small soluble copper protein which plays an important role in regulating copper
homeostasis. The protein is known to confirm two separated binding sites with high affinities for
Cu2+ and Cu+, which locate at the two opposite sides of the hydrophobic barrel. The role of CopC in
copper trafficking is an open question.In this paper, The interaction of Cu(), Cu(), Cd(), Ag()
with apoCopC and BSA was studied by cyclic voltammetry and electrochemical impedance
spectroscopy based on apoCopC and BSA immobilised on gold electrode and glassy carbon
electrode.Firstly, An electrode modified with protein monolayer was prepared by the self-assembly of
apoCopC at a gold electrode. The interaction of apoCopC with Cu() had been investigated by cyclic
voltammetry at apoCopC-modified gold electrode. The oxidation peak and reduction peak of Cu2+ at
apoCopC-modified gold electrode had been found. The peak potential s of Cu2+ at apoCopC-modified
electrode shifted evidently comparing with that at the bare gold electrode, indicated that apoCopC
could adsorb on the electrode firmly and Cu2+ bound to apoCopC to form a complex. The
voltammetric behavior of Cu2+ on the apoCopC/Au electrode was studied by scan rate, accumulation
time, various concentration and presence of TNS. The electron transfer coefficient() was 0.5, electron
transfer rate constant (ks) was 0.75 s-1. The surface concentration of Cu2+-CopC () was 2.710-10
molcm-2 which showed it was monolayer modified. The oxidation peak and reduction peak of Cd2+
at apoCopC-modified gold electrode had been found. The peak potentials of Cd2+ at apoCopCmodified electrode shifted evidently comparing with that at the bare gold electrode, indicated that
Cd2+ bound to apoCopC to form a complex. The oxidation peak and reduction peak of Cd2+ at
apoCopC-modified gold electrode was controlled by diffusion and adsorpti on. The surface
concentration of Cd2+-CopC () was 1.310-10 molcm-2 which showed it was sub-monolayer
modified.Secondly, the glassy carbon electrode modified with apoCopC was prepared and the
adsorption of apoCopC on electrode was studied. The electrochemical behavior of Cu( ), Cu(),
Ag() on apoCopC-modified electrode was studied. It was found that the reduction peak potential of
Cu2+ and the formal potential of Cu2+/Cu+ redox couples shifted negatively, at apoCopC-modified
glassy carbon electrode, comparing with that at the bare glassy carbon electrode. These indicated that
Cu2+ could bind apoCopC via non-hydrophobic (electrostatic and/or covalent) interaction. The
reduction peak potential of Cu() and the formal potential of Cu+/Cu redox couples shifted
negatively comparing with that at the bare glassy carbon electrode, indicated that Cu() could bind
apoCopC via non-hydrophobic (electrostatic and/or covalent) interaction. The reduction peak potential
of Ag+ and the formal potential of Ag+/Ag redox couples shifted negatively comparing with that at
the bare glassy carbon electrode, indicated that Ag() could bind apoCopC via non-hydrophobic
(electrostatic and/or covalent) interaction.Finally, BSA-modified gold electrode/glassy carbon
electrode was prepared. The interaction of Cu(), Cu(), Cd(), Ag() with BSA was studied by
cyclic voltammetry and electrochemical impedance spectroscopy. The electrochemical behavior of
Cu(),Cd() at BSA-modified gold electrode was studied. Cu(), Cu(), Ag() could bind BSA
via non-hydroph obic (electrostatic and/or covalent) interaction.