o When the ATP levels are high, than the Electron Transport Chain is inhibited. This will
reduce amount of NADH is used and causes an increase in NADH.
o Both high levels of NADH and ATP stop the Critic Acid Cycle, which stop using AcetylCoA. This allows an abundance of Acetyl-CoA build up in the mitochondria.
Palmitate synthesis
o High insulin levels causes an increase of Acetyl-CoA Carboxylase and Fatty Acid
Synthase (FAS).
o This reaction is an endogenic.
o The decarboxylation of Malonyl-CoA repeats until the desires number of fatty acid is
reach.
o Malonyl-CoA is being decarboxylation and the Acetyl group is begin reduced, from this
process these two molecules is combined together.
Palmitate Oxidation
o 14-20 fatty acid are hard to get into the Mitochondrial Matrix.
o This process depends on the concentration in the blood and will not go under low
concentration.
o Also, Malonyl-CoA can inhibition the Carnitine Transporter, which prevents the Betaoxidation.
Ketolysis
o Acetoacetate picked up form the blood is activated in the mitochondria by succinyl-CoA
acetoacetyl-CoA transferase (Thiophorase), an enzyme present only in tissue outside the
liver. During this reaction 3-hydroxybutyrate us oxidized to acetoacetate. The liver lacks
this enzyme, so it can't catabolize the ketone bodies that is produces.
Protein Absorption
o Absorption in Small Intestine ( the lumen)
The amino acids cross the intestine lumen through active transport (Na+).
sodium are pump into the cell and protons are pump out.
At the basal membrane amino acids undergoes simple diffusion into the
blood stream. However, dipeptides and tripeptides undergoes facilitated
diffusion (H+)
Protein As Energy
In Fasting State
o When other energy sources are low, furthermore, protein can be used for energy.
o De-amination and transamination involves the process of removing amino group from the
carbon backbone.
Glucogenic vs. Ketogenic
Glucogenic (Glucogenic amino acids---> glucose---> glycolysis)
o serine
o glycine
o alanine
o arginine
o histidine
o glutamine
o methionine
o asparagine
o glutamate
o aspartate
o cysteine
o proline
o valine
Ketogenic (Ketogenic amino acids ---> acetyl-CoA---> Citric acid cycle)
o Leucine
o Lysine
Both
o
o
o
o
o
Threonine
isoleucine
phenylalanine
tryptophan
tryosine