126
66.5
11
126
66.5
6-10
29
BX51WI
6-10
40
74
.1
9.5
483
50*
62
150.4
1 503.8
45
164
8.2 17.8
Focus stroke
29.1 41 80 43
201
50
375.5
483
Focus stroke
Objective
mounting
position
43
102
430
40
187
172.8
430
172.8
368
9.5
11
12
9.
5
12
4.3
12
4.3
11.5
18
11.5
18
1 503.8
45
164
482
407.9
205
199
186
185
168.5
149
120
90
73
150
200
224
240
250
317.5
40
74
90
73
15
14
7.5
14
7.5
29
(unit: mm)
15
11
482
408
205
199
186
185
168.5
149
120
BX61WI dimensions
150
200
224
240
250
317.5
BX51WI dimensions
567
408
21.5
90
190.5
317.5
408
567
* shows the stage's up and bottom position
Weight: approx. 19 kg
Weight: approx. 21 kg
BX51+WI-DPMC dimensions
45.5
21.5
76.2
90.8
219.5
50*
Objective
mounting
position
150.4
1 498.5
75
133.9
7.2
18.8
Focus Stroke
29.1 90.9 43
12 78.9
229.7
(unit: mm)
96.4
367.9
482.9
50 shows the stage's available up and
bottom position.
40
96
201
90
190.5
317.5
408
567
Weight: approx. 25 kg
BX61WI
A dual commitment:
Preventing vibration and
protecting living cell specimens
One design theme was central to the development of the new fixed stage microscopes from Olympus
achieve an evenhigher standard of stability and reliability in electro-physiological applications.
The result is a wide range of advanced new features to avoid and prevent vibration. These innovations
include the introduction of a new observation method along with detailed analysis of operability and
further refinements in image clarity. These improvementswork together to make patch clamp
operations smoother and more efficient than ever before.
Combined with the traditional excellence of UIS2 optics, the new Olympus fixed stage microscopes
define new levels of quality in both performance and ease of use.
New
concept
Full-system physiological
confocal microscope,
BX61WI with Z-axis motor
is LSM ready
0
.
1
.
N.A LN20xW
Variable Click-stops
All click-stops, as when selecting between camera and observation
modes, can be adjusted to the point of no click and thus no
vibration.
Excitation light
Fluorescence light
PLF
M
U
L
X
Dual port
WI-DPMC
Video port
FL/DIC
Observation port
FL/DIC
Dichroic mirror
Intermediate
magnification
change lever
IR-DIC
IR-DIC port
IR-DIC
Transmitted light
Analyzer
C mount
4x lens
0.35x(0.25x) lens
Light path exchange lever
Tube lens
Mirror unit exchange turret
Mirror unit
DIC Prism
80 Intermediate magnification 4 x
XLUMPLFLN20xW
N.A. 1.0
Condenser
DIC element
Examples using
intermediate
magnification
changer WI-DPMC
IR-DIC observation, trigeminal motoneuron, Tomio Inoue Ph.D, Department of Oral Physiology, Showa University
3
44
q Vibration-free shutter
The fluorescence shutter slides horizontally
with no detents and no vibration.
u
t
w Mirror unit turret with
adjustable click release
The click-stop on the 6 position turret can
be released with a precision screwdriver.
y A waterproofing sheet
A waterproofing sheet, attached by the
supplied magnets, provides protection
against liquid overflow and spills.
The sheet is large enough to protect
the frame, condenser and focusing
mechanisms .
BX51WI+IX-SVL2
Microinjection
W.D.
WI-ARMAD
W.D.
W.D.
18 mm
18 mm
40 mm
40 mm
BX51WI+WI-STAD+U-SVRB-4
18 mm
50 mm
Normal configuration
Photoactivation
A variety of convenient units to
add light sources and control the light
Removable AS/FS
(photo shown is BX-RFA)
BX-RFSPOT
XLFLUOR2x/340 XLFLUOR4x/340
Nucleus of solitary tract from slice of rat medulla oblongata (thickness: 400 m)
Prof. Fusao Kato
School of Medicine Physiology Dept.,
Jikei University
Kato & Shigetomi, J. Physiol.(2001), 530: 469-486
Analyzer
DIC prism
Objective
DIC prism
U-UCD8
WI-DICT
WI-TP137
Rotatable
Measuring changes in
membrane electric potential by
using the XLUMPLFLN20xW
objective with N.A. 1.0
The XLUMPLFLN20xW objective, with
its high N.A., and 2.0 mm of working
distance allows the measurement of cell
membrane electric potential (as seen
right). Also, the 4x macro objective
(XLFLUOR4x/340) can be used to
measure membrane potential
at the tissue level. A water immersion
cap (XL-CAP) can be attached to the
macro 2x or 4x objectives to eliminate
disturbances caused by water ripples.
Takashi Tominaga Ph.D, Brain-Operative Device Lab., Brainway Group, Brain Science Institute, Riken
Adjustable
*The above data were obtained using a forerunner to the XLUMPLFLN20xW, but the XLUMPLFLN20xW can be used to
perform the same operations.
10
Accessories
BX51WI/BX61WI specifications
Optics
Material
Illumination
U-DPTS
BX51WI
BX61WI
Light source
BX-UCB
Focus
BX51WI
BX61WI
U-ECA
U-CMDPTS
U-CA
Camera for IR
Dichromatic mirror
U-DPTS
Camera control unit
General camera
Monitor
Microscope
body
Light source
TH4
Microscope
body
Nosepiece
WI-SRE3
U-SLRE
WI-SNPXLU2
WI-SSNP
Fluorescent BX-RFA
illuminator
BX-URA2
Observation U-TR30-2
tube
U-ETR-4
WI-DPMC
Stage
Tube lens
IX-SVL2
Analyzer
U-TVCAC
Mirror unit
Long
working
distance
condenser
Excitation light
DIC prism
Water immersion objective for IR
Depth
100 150 m
WI-DICD
WI-OBCD
WI-XYM dimensions
(unit: mm)
7 hole
10
10
11.3
8
Transmitted light
105 60
275
355.5(14in)
380
M6 depth 6
3 steps BI:
100%
Front port 100%
Simultaneous use of front (visible light) and back (near IR light) ports
Clicker ability adjustable
Binocular
F.N. 22, inclination angle 24,
observation tube
Interpupillary distance: 50 76 mm
Front port
For fluorescent observation,
F.N. 22,
Simultaneous adjustment function with backport
Back port
For IR observation (c mount adapter)
Magnification
Magnification changer
Total
N.A.
Projection
change
lens magnification
magnification
magnification
* custom made
0.25x
5x
0.15
1/2 inch
product
0.35x
7x
0.22
2/3 inch
(when combining with
0.5x*
10x
0.4
2/3 inch
XLUMPLFLN20xW)
1x*
20x
0.95
2/3 inch
2x*
40x
0.95
2/3 inch
4x
80x
0.95
2/3 inch
IR-DIC observation Insert 775 nm analyzer or 900 nm analyzer into WI-DPMC
55
77
40
58
11
10
56
38
40
70
78
11.3
Objectives
Optical
light path
exchange
9 hole
216
200
33
150
108
Condenser
7 hole
WI-DPMC specifications
28
Brain slice
300 400 m
WI-UCD
68
100
28
68 30 160
Monitor
WI-XYS
U-SVL(R)B-4
(unit: mm)
11
775 nm
900 nm
200
11xP25=275
400
110
20.7
105
100
50
12.5
WI-XYS
50 12
Visible light
40
58
209
271
W.D. (mm)
20.0
3.5
3.5
3.3
2.0
1.5
21.0 (include 5 mm water)
29.5 (include 5 mm water)
2.0
1.8
N.A.
0.10
0.30
0.50
0.80
1.00
1.10
0.14
0.28
1.00
20
MPLN5x
UMPLFLN10xW
UMPLFLN20xW
LUMPLFLN40xW
LUMPLFLN60xW
LUMFLN60xW
XLFLUOR2x/340
XLFLUOR4x/340
XLUMPLFLN20xW
50
300
Stage height
Manipulator setting
Material
Weight
Applicable
anti-vibration table
50
Objectives
No cover objective
Long working distance no cover water immersion
objective
50
7 hole
WI-XYM Microscope
X-Y stroke
Maximum loading
Weight
Applicable
anti-vibration table
100
180
12xP25=300
380
50
WI-XYS dimensions
50
12
BX51WI, BX61WI
25 mm (minimum graduation 10 m)
294 N (30 kgf)
Approx. 9.1 kg
25 mm or 50 mm pitch with M5, M6 screw holes
1inch or 2 inch pitch with 1/4-20UNC screw holes
Fixable
271 mm or 221 mm
Fixable to 7 mm or 25 mm pitch holes with
M5 or M6 bolts and nuts
Iron (magnet can be used)
Approx. 22.1 kg
25 mm or 50 mm pitch with M5, M6 screw holes
1inch or 2 inch pitch with 1/4-20UNC screw holes
U-CMDPTS
C-mount adapter for
U-DPTS
U-PMTVC2XIR*7
U-PMTVC4XIR*7
C-mount camera port
U-DPCAD
Dual port tube with
C-mounts
U-TVCAC*7
C-mount camera U-TV0.35XC-2
C-mount camera
magnification
port tube with
change unit
0.35x lens
U-SPT
Single port
tube
U-DPTS
Multi double port tube
* WHN10X
WHN10X-H
CROSSWHN10X
Eyepiece
U-CT30
Centering eyepiece
U-SMAD
Sony
mount adapter
U-TMAD
T-mount adapter
U-TV0.5XC-3
C-mount camera
port tube with
0.5x lens
U-BMAD
Bayonet
mount adapter
U-TV0.63XC
C-mount camera
port tube with
0.63x lens
U-TV1X-2
Direct image camera port
C-mount camera
*
U-ETR-4*1
Erect image trinocular tube
U-TR30IR
Trinocular tube for IR
WI-DPMC*6
Double port
magnification
change unit
(DPMC4X included)
U-TR30-2
Trinocular tube
DPMC0.25X
0.25X adapter lens for WI-DPMC
DPMC0.35X
0.35X adapter lens for WI-DPMC
U-ANT
Analyzer for transmitted light
U-ECA*3
Magnification
changer 2X
U-FWO
Observation
filter wheel
(32 filter)
WI-ANTIR
IR analyzer for transmitted light
C-mount camera
(2/3" or less)
U-DP*2
Dual port
U-CA*3
Magnification
changer
U-CMAD3
C-mount adapter
U-KPA
Intermediate attachment for
simple polarizing observation
U-TRU*2*4
Trinocular
intermediate attachment
U-DP1XC
Dual port 1X
DPMC-AN
Analyzer for WI-DPMC
DPMC-ANIR
IR analyzer for WI-DPMC
U-AN
Analyzer for reflected light
WI-RSH
Reflected light shutter
WI-ANIR
IR analyzer for reflected light
U-RLGAD
Light guide
adapter
FV5-FIR
Fiber
LG-ULHAD
Lamphouse adapter
U-RFSS
Rectangular
field stop
BX-ARM
Arm for transmitted light
Mirror unit
BX-RFA*5
BX fluorescence illuminator
BX-URA2*5
Universal reflected light
illuminator
IX-SUSP
Stage plate
BX-RFAA*5
Motorized
fluorescence
illuminator
BX-RFSPOT
Pinhole unit
U-LHAD
Lamphouse adapter
U-RFL-T
Power supply unit for
mercury lamp
U-DP*2
Dual port
U-RMT
Extension cord
WI-FSH
Fixed stage adapter
45SCF
Heat reflection filter
WI-DICTHRA2
High resolution
DIC prism
WI-DICT2
DIC prism for
transmitted light
U-SLRE
Slide nosepiece
WI-SRE3
Swing nosepiece
WI-SNPXLU2
Single positioin nosepiece
U-TLD
Dry top lens
WI-RMSAD
RMS adapter
XLFLUOR2X/340
XLFLUOR4X/340
Fluorescence objectives for
low magnification
XL-CAP
Water immersion lens
for XL objective
MPLN5X
UMPLFLN10XW
UMPLFLN20XW
LUMPLFLN40XW
LUMPLFLN60XW
LUMFLN60XW
Objectives
U-UCD8*8
8 position
universal
condenser
XLUMPLFLN20XW
20X super high N.A.
objective
WI-TP137
Quarter wave
tint plate
WI-OBCD
Long
working distance
oblique condenser
U-DIC10
U-DIC10S
U-DIC20
U-DIC40
U-DIC60
Optical element
WI-DICD
Long
working distance
DIC condenser
WI-UCD
WI-UCD
Long working
Swing-out
distance universal condenser
condenser
WI-DIC10HR
WI-DIC20HR
WI-DIC40HR
WI-DIC60HR
WI-DICXLU20HR
Optical element
*1 Slight vignetting may occur in the periphery of the field of view in combination with an additional intermediate attachment. *2 Slight vignetting may occur in the periphery of the field of view in combination with
*5 F.N. is 22 when fluorescence observation. *6 Use with fluorescent illuminator. *7 Acceptable camera adapters are U-TV1X-2, U-TVACA, U-PMTVC2XIR and U-PMTVC4X. *8 U-UCD8 cannot be used with WI-STAD.
13
U-LH100HGAPO
100 W mercury apo
lamphousing
U-LH100-3
100 W halogen
lamphousing
BX51WIF
Fixed stage upright
microscope main body
BX61WIF
Motorized frame
WI-SSNP
Swing-slide nosepiece
WI-NPA
Nosepiece arm
U-LH100HG
100 W mercury
lamphousing
For frame
WI-ARMAD
Arm height
gaining Kit
U-RX-T
Power supply unit for
xenon lamp
U-FWR
Reflected filter wheel
U-SVL(R)B-4
Mechanical stage
WI-STAD*8
Stage adapter
U-LH100XEAPO
100 W xenon apo
lamphousing
32BP775
775 nm
band pass filter
32IR900
900 nm filter
32PO
32 filter
Polarizer
32POIR
IR polarizer
TH4-HS
Hand switch
TH4
External
light source
U-LH100IR
100 W halogen
lamphousing for IR
WI-XYS
Bridge stage
BX61WIF
U-IFFH
Focus handle
interface
WI-XYM
Microscope mover
BX-UCB
Control box
U-FH
Focus handle
Motorized unit
Manipulator
fluorescence illuminator. *3 Can be used with U-ETR-4 and U-TR30IR. Field of view is limited when using more than two intermediate tubes. *4 Sub port can accept U-TV1X-2, and 2/3" or less CCD cameras.
14