I

GOAL
The goal in this trial is to establish the levels of vitamin C (ascorbic acid) is
iodimetri.
II
THEORETICAL BASIS
Quantitative analysis is an analysis with regard to the determination of how much a
particular substance contained in a sample. The specified substances, referred to as
a constituent or analyte, construct either a small portion or most of the samples
analyzed. If the substance is analyzed make up more than 1% of the sample, then
the analyte is considered as the main constituent. A substance is considered as
minor constituents if the amount between 0.01% to 1% of the sample (Underwood,
2002).
Volumetric analysis is one method of quantitative analysis, which is a very
important use in determining the concentration of a substance present in
larutan.keberhasilan volumetric analysis is largely determined by the existence of
appropriate indicators so as to indicate the appropriate endpoint (Harjanti, 2008).
Iodimetri is titrimetric analysis of substances reducing agents such as sodium
thiosulfate, arsenic using standard iodine solution directly. Iodometri is titrimetri
analysis for substances reductant with the addition of standard iodine solution with
the addition of redundant and excess titrated with standard sodium thiosulfate
solution. Iodimetri titration titration using a solution iodum its reduction oxidation.
That is iodometric titration oxidant is added to a solution of excess potassium iodide
and iodine are released (equivalent to the amount of oxidant) ditirasi with a
standard solution of sodium thiosulfate (Irjawati, 2012).
Vitamin C or L-ascorbic acid is an acidic compound with the empirical formula of
molecules = 176.13. Vitamin C is used as an anti-oxidant to form collagen and helps
maintain capillaries, bones and teeth. Levels of vitamin C in solution using redox
titration iodimetri dengaqn indicator solution using starch (starch) by adding
piecemeal solution of iodine (I2) are known molarity to achieve a balance point
which is marked by changes in the color of the solution became dark blue (Pratt et
al, 2011) ,
Vitamin C (acid oskorbat) is one of the vitamins needed by the human body. Lack of
vitamin C has been known as sprue disease with symptoms such as bleeding gums,
sore tongue, muscle and joint pain, weight loss, lethargy and others. Vitamin C has
an important role for the human body as in the synthesis of collagen, camitime
formation, involved in the metabolism of cholesterol to bile acids and also play a
role in the formation of the neurotransmitter norepinephrine. Vitamin C has
antioxidant properties that can protect the molecules that are needed by the body,
such as proteins, lipids, carbohydrates dam nucleic acids from damage by free

radicals and reactive oxygen species (Arifin et al, 2007).

Vitamin C is easily oxidized when exposed to air and this process is accelerated by
heat, light, alkali, enzymes, oksaidator, as well as a catalyst of copper (Cu) and iron
(Fe). These things cause problems if there is the effect of temperature and storage
time for vitamin C in guava. For that we need evidenced by a study on the
determination of levels of vitamin C in guava stored in the guava fruit is stored in a
certain period of time and at different temperatures (Masfufatun et al, 2009).
L-ascorbic acid in the presence of ascorbic acid oxidase enzyme oxidized to Ldehydroascorbic acid. This acid is also chemically very unstable and undergo further
changes to acid L-diketogulonat who no longer have a liveliness as vitamin C.
Atmosphere bases cause Ldiketogulonat acid is oxidized to oxalic acid and L-treonat
(Safaryani et al, 2007).
III
TOOLS AND MATERIALS
1. Tool
Tools that are used in this experiment are:
Trunk mixer
50 ml Buret
100 ml glass chemistry
100 ml glass chemistry
Hot plate
250 ml Erlenmeyer flask
Drop pipette
the stand and clamp
analytical Scales
Filler
2. Materials
Materials used in this experiment are:
distilled water
dilute sulfuric acid (H2SO4)
Solution Iodine (I2) 0.1 N
Kanji solution 0.5%
Vitacimin
IV.
WORK PROCEDURES
Vitacimin
The crushed until smooth
Weighed as much as 0.4 grams
Diluted with distilled water

 Added 5 drops of dilute sulfuric acid

Vitacimin solution
Taken 5 ml
Entered into a 250 ml Erlenmeyer
Added 5 drops of starch indicator
titrated with iodine solution to change color

N
O
1
2

TREATMENT
RESULTS
Sample dilute
H2SO4 + water
Titrated
premises KI 0.1N

Titrated
premises
Clear solution

.V.
OBSERVATION RESULT
1. Observation Data

Blue solution

2. Data calculation
Levels vitacimin
Dik: Vi2 = 9 ml
BE = 8.806 mg
Mg of sample: 400 mg
Ni2 = 0.1 N
Dit: Levels of Vitamin C = ... ..?
Solution:
Levels of Vitamin C = VI2xNI2xBE
mg sample
= X 100%
= X 100
= 1.98%

VI.
DISCUSSION
Iodimetri a titration with I2 as peniter. In redox reactions should always be oxidizer
(which experienced a reduction) and reducing agents (which undergoes oxidation).
Because if an element increasing oxidation number (releasing e) there must be an
element of reduced oxidation number (capturing e). So it may not only be an
oxidizing or reducing agent only.

In this experiment, is the assay of Vitamin C with iodimetri method that uses a
starch indicator. Indicator starch is more commonly used, because of the deep blue
color of starch-iodine complex serves as a test of sensitivity to iodine. Kanji reacts
with iodine, in the presence of iodide to form a blue complex strong, which will look
at iodine concentrations were very low.
Kanji can not be used in highly acidic medium because it will occur on starch
hydrolysis itself. Excellence in the use of this kanji is that it's cheap, but there are
weaknesses that are not soluble in cold water, the instability of the suspension in
the water and with the iodine to give a compound that is not soluble in water, so
that the starch should not be added too early in titration (therefore, in titrasiiod
starch solution should not be added until just before the end point, when the color
begins to fade).
Iodine is an oxidizing agent that is not too strong (weak), so that only substances
which are strong reducing agent that can be titrated. The indicator used is starch
which will provide a blue color at the endpoint. I2 solution made by dissolving pure
I2 further standardized with Na-thiosulfate. dissolved in sodium hydroxide and then
neutralized by the addition of acid. Due to the solubility of iodine in water of little
value then the solution prepared by dissolving I2 I2 in KI solution, thus the actual
state is used to titrate solution I3. At the time of the oxidation reaction, iodine is
reduced to iodide will be in accordance with the reaction.
In the treatment we did was the assay of ascorbic acid compound in preparation
Vitacimin. 0.2 gram sample added with distilled water and 5 drops of dilute sulfuric
acid. Dilute sulfuric acid that serves as a catalyst to accelerate the reaction without
reacting participate in the sample resulting in a pure reaction products between
ascorbic acid and iodine as well as indicator. Once added as much as 5 drops of
starch indicator and titrated with a standard solution I2 standard 0.1 N dropwise.
Results obtained at the end point of the titration is the original sample solution is
yellow after adding 0.1 N I2 changes to dark blue. With the reaction between
ascorbic acid and I2 are:
From the calculation result data levels of ascorbic acid compounds in samples
Vitacimin C result. Ascorbic acid levels in preparation Vitacimin C by 43.14%.

VII.
CONCLUSION
Based on the experiments that have been carried out it can be concluded that:
Assay of Vitamin C by the method of preparation iodimetri on Vitacimin C is 1.98%

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