reactions
Changes of the HMS under different
cellular conditions
Tissue specific activity of the HMS
Carbohydrate Metabolism
Function
NADPH
Reducing power
carrier
Synthetic pathways
Role as cellular
antioxidants
Ribose
production
synthesis
Oxidative phases
Reactions
producing
NADPH
Irreversible
Non-oxidative phases
Produces
ribose-5-P
Reversible reactions
feed to glycolysis
pathways
Production of superoxide
Hb-Fe2+-O2
Antioxidant enzymes
Superoxide
dismutase
Glutathione peroxidase
Glutathione reductase
The pentose
pathway can be
divided into two
phases.
Non-oxidative
interconversion of sugars
NADPH + H+ is formed
from two separate
reactions.
Regulatory enzyme
5 carbon atoms
Regulatory enzyme
Transketolase
Ingested ribose
can enter the
glycolytic pathway
through the
pentose pathway.
More NADPH is needed than ribose 5phosphate; Fatty acid synthesis in adipose cells.
What is glutathione?
Why is it important?
NADPH is a
phosphorylated
form of NADH.
In general, with some
exceptions, NADH is
used to drive the
phosphorylation of ADP
to ATP. NADPH is used
where reducing potential
is required for synthetic
reactions.
Glutathione is a tripeptide
composed of glutamate,
cysteine, glycine.
Reduced glutathione
(GSH) maintains the
normal reduced state
of the cell.
Reduced
glutathione (GSH)
Glutathione Functions -1
It serves as a reductant.
Conjugates to drugs making them
water soluble.
Involved in amino acid transport
across cell membranes.
Cofactor in some enzymatic
reactions.
rearrangement
bonds.
of protein disulfide
Glutathione Functions -2
The enzyme
glutathione reductase
uses NADPH as a
cofactor to reduce
GS-SG back to two
moles of GSH. Thus,
the pentose pathway
is linked to the supply
of adequate amounts
of GSH.
- pamaquine
purine glycoside from fava beans.