Quest Animal Health and Biosecurity, 15609 Peace Valley Rd., Brookwood, AL 35444; and Department of
Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, 240 Wise
Center Dr., P.O. Box 6100, Mississippi State 39762
INTRODUCTION
Performance standards conducted at poultry processing plants for the detection of Salmonella were developed to reduce the health risk to consumers posed by
Salmonella contamination of broiler carcasses. The efficacy of the performance standards relies on accurately
measuring the Salmonella status of the broiler carcasses
being sampled. The whole carcass rinse (WCR) procedure is the most common method used to determine Salmonella status in broiler carcasses and has
been widely used in studies of Salmonella prevalence in
broiler carcasses (Cason et al., 1997; Cox et al., 1983;
C 2015 Poultry Science Association Inc.
Received March 18, 2014.
Accepted November 5, 2014.
1
Corresponding author: wills@cvm.msstate.edu
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Salmonella Isolation
Sample Size
For each of the carcass rinse method trials, 150 pairs
of broiler carcasses or split-carcass halves were collected. Power analysis using Cantors sample size equation (Cantor, 1996) estimated that for each trial, the
sample size of 150 pairs would allow the discrimination
between kappa values for 2 methods of 0.4 and 0.6 assuming an level of 0.05, power of 0.80, and prevalence
estimates of 0.50 by either method.
Statistical Analysis
Assessing agreement and differences in prevalence for
the different methods was performed using the FREQ
procedure of SAS for Windows version 9.3 (SAS Institute, Inc., Cary, NC). The Tables/Agree statement generated the kappa coefficient of agreement between adjacent and rinse samples for the adjacent pair method,
between rinse and rerinse samples for the repeat rinse
method, and between half A and half B for the split
carcass method within each of the appropriate trials.
Table 1. Results of kappa agreement, prevalence, and McNemars test for Salmonella status of split carcass, repeat rinse (rinse
and rerinse), and adjacent pair (rinse and adjacent) rinse sampling methods.
kappa
Split carcass
Trial
1
2
3
4
5
150
150
150
150
150
Repeat rinse
Prevalence
Adjacent
0.58
0.45
0.64
0.47
0.41
0.46
0.55
0.46
Split carcass
Half A
Half B
81.3
63.3
78.7
70.7
McNemars test
Repeat rinse
Rinse
Adjacent
Rerinse
P-value
Split carcass
Repeat rinse
Adjacent
0.108
0.398
0.002
1.000
0.853
0.117
0.371
0.071
24.7
69.3
22.7
20.0
66.0
12.0
The test of the null hypothesis that kappa = 0 was < 0.0001 for all comparisons.
24.7
68.7
17.3
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GALARNEAU ET AL.
Overall Comparisons
Although prevalence can affect agreement as measured by kappa, wherein paradoxically, extremely high
and extremely low prevalence would result in lower
kappa coefficient, this was not observed in this study.
Even though there was substantial variation in prevalence among trials, there were no extremely high or
low prevalence estimates. Considering the results of the
analyses, the highest and lowest kappa agreement was
observed among repeat rinses of the same carcass. The
highest, or substantial, agreement (kappa = 0.64) was
observed in the repeat rinse method in trial 3; the least,
or moderate, agreement (kappa = 0.41) was found also
in the repeat rinse method in trial 5. On the other hand,
the adjacent pairs rinse method showed a consistently
moderate kappa agreement without any significant differences in prevalence.
The repeat rinses (rinse and rerinse) of the same
carcass showed inconsistent agreement among 3 trials
ACKNOWLEDGMENTS
This research was supported by USDANational Integrated Food Safety Initiatives (USDA-NIFSI) award
number 2006-51110-03645. Karen Galarneau is grateful
to the Fulbright Philippines Fellowship Program and
the International Institute of Education.
The authors are grateful to Terry Doler, Food Safety
Laboratory, College of Veterinary Medicine, Mississippi
State University, for leading the laboratory testing.
They are also grateful to Mary Ann Ballard, Adam
Pochocki, Kelly Hataway, Kevin Howe, and Sanaz
Salehi for their efforts in sample collection.
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