11 May 2015
Introduction
The genus Salvia, with about 900 species throughout the
world, as one of the most widespread members of the
Lamiaceae family. Although Salvia species are abundant
in the Turkish flora, as being represented by 88 species
and 93 taxa and 45 of which are endemic [1], only
biological activities and bioactive properties of a few are
available in the literature while most have yet to be tapped
into. Among them, the species evaluated here are aromatic
plants widely distributed in Anatolia and Mediterranean
region where they are commonly known as adacayi,
salba and/or dadirak by the locals. They are
commonly used in local folk medical practices [2]. Plants
evaluated here together with the other members of the
genus Salvia used in diarrhea, gonorrhea and hemorrhoids,
eye diseases and as an antiseptic, antispasmodic and
stomachic[3]. Additionally, many Salvia species are used
in herbal tea and for food flavouring, as well as in
cosmetics, perfumery and the pharmaceutical industries
throughout the world [4].
24 | P a g e
were done at 25 C.
Plant samples
Antioxidant activity
Kahramanmara
Turkey.
Preparation of extracts
acetone (pi: 5.1), ethanol (pi: 5.2) and methanol (pi: 5.1)
Filter-sterilized
Sutcu
and
Imam
University,
concentrated
extracts
were
to 734 nm. 10, 20, 40, 80, and 200 l extracts were added
to 4.0 ml ABTS.+ solution and absorbances were measured
at 734 nm after 2 h. The percentage of free radical
scavenging
effect
was
calculated
as
follows:
25 | P a g e
Statistical analysis
different extracts.
Antimicrobial activity
The antimicrobial activities of extracts were determinated
Results
Plant
Locality
Coordinate
samples
Salvia
Ardahan
N41
verticillata
District/
07.13
L.
Around Kuru
E042
River
41.55
for
Candida
albicans
ATCC
10231
and
Altitude
time
except
Harvest
var.
amasiaca
23.06.2011
1900
25.06.2011
1960
(Turkey)
Salvia
Ardahan
N41
microstegia
District
13,49
Boiss
(Turkey)
E042
&
43.01
Bal.
Rutin
(g/ml)
Myricetin
(g/ml)
Quercetin
(g/ml)
Kaempferol
(g/ml)
Katesin
(g/ml)
Naringin
(g/ml)
Naringenin
(g/ml)
Resveratrol
(g/ml)
0.5
75.6
122.95
350.45
292.3
0.7
36.25
3.65
Salvia microstegia
0.5
11.9
43.6
0.45
315.5
0.5
227.2
0.5
26 | P a g e
Table 3. DPPH and ABTS free radical scavenging activi of Salvia species
Plant samles
DPPH (%)
ABTS (%)
10 l
20 l
40 l
80 l
200 l
10 l
20 l
40 l
80 l
200 l
90.49
86.50
78.71
69.39
27.38
98.37
98.57
99.18
99.38
84.37
Salvia microstegia
99.81
99.81
91.64
57.41
14.18
98.78
98.57
98.78
99.39
97.55
Antioxidant activity
Stalk
Leaf
Flower
A
P
Microorganisms
Antimicrobial activity (mm)
C H A
B. subtilis
ATCC
6633
E. aerogenes
ATCC
13048
E. cloacae
ATCC
13047D
M. luteus
NRLL B4375
S. aureus
ATCC
25923
E. coli
ATCC
11229
C. albicans
ATCC
10231
S. cerevisiae
C H A E
172
0.
33
15
0.
33
33
0.
57
29
0.
57
23
0.
57
23
0.
0
24
0.
0
25
1.
73
18
0.
88
15
0.
57
16
0.
33
16
0.
57
39
0.
57
38
0.
33
22
0.
57
26
0.
33
15
0.
33
-
24
0.
0
21
0.
0
18
0.
33
19
0.
57
32
0.
0
22
0.
88
41
0.
57
44
0.
33
21
0.
33
-
24
0.
88
14
0.
57
-
15
0.
00
-
17
0.
33
-
22
2.
88
-
14
0.
33
18
0.
57
16
0.
57
-
13
0.
33
31
0.
57
18
0.
33
-
14
0.
33
38
0.
88
18
0.
33
-
: No inhibtition zone, 2: nhibition zone (mm), AP: Aerial part, C: Chlorophorm, H: Hexane, A: Acetone, E: Ethanol, M: Methan
27 | P a g e
Stalk
Microorganisms
B. subtilis
ATCC 6633
E. aerogenes
ATCC
13048
E. cloacae
ATCC
13047D
M. luteus
NRLL B4375
S. aureus
ATCC
25923
E. coli
ATCC
11229
C. albicans
ATCC
10231
S. cerevisiae
Leaf
Flower
AP
H
-1
A
15
0.
33
-
E
-
19
0.
00
17
0.
57
16
0.
33
18
0.
00
16
0.
00
-
15
0
.0
-
16
0.
33
15
0.
0
M
26
1.
15
21
0.
88
C H A E
- - - -
M
16
1.
15
-
16
0.
00
25
0.
33
20
0.
00
C H A E
- - - -
M
14
0
.0
-
12
0.
00
C H A E
- - - -
M
18
1.
15
20
3.
46
M
24
0.
88
19
0.
57
12
0
.0
17
0.
00
14
0.
00
22
0.
57
-
: No inhibtition zone, 2: nhibition zone (mm), AP: Aerial part, C: Chlorophorm, H: Hexane, A: Acetone, E: Ethanol, M: Methanol
Salvia
radical
verticillata
microstegia
L.
var.
exhibited
amasiaca
similar
(97.55
free
and
84.37
microorganisms.
Antimicrobial activity
Additionally,
chloroform,
hexane,
28 | P a g e
Discussion
secondary
microorganisms
Major
verticillata
Plant-derived
compounds
(167.1
are
mgGAE/g
mostly
DW)
than
Salvia
at
become
in
an
this
study.
antimicrobial
21, 22, 23, 24]. The results of this study showed that
effects
Candida
has
rates
on
agents
different
increasingly
albicans
and
Saccharomyces
and
Conflict of Interest
References
1
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10
11
12
13
14
15
16