Purifine PLC

Team up with DSM for smart fruit and vegetable

processing

Significantly
increase oil yields

and improve operational efficiency

Enzyme innovations have the power to change the world. In the quest to make the most of our
natural resources, industry must be one of the guardians of the planet. Demand for seed oils,
such as soya, canola and sunflower have never been higher. For the most sustainable and
profitable production, its vital to extract the maximum oil from the raw materials. Purifine PLC
phospholipase C (PLC) from DSM provides a solution which increases yield, quality and value
without using more natural resources.

For producers of bio-oils, using Purifine PLC smart

enzyme technology means increased output and
reduced waste. And for the planet, smart enzymes in
bio-oil production mean less reliance on chemicals,
and lower water and energy consumption.
Purifine PLC is an enzymatic degumming solution
that can be integrated into most plant designs, and
has proven yield gain benefits when compared to
water degumming and other degumming enzyme
alternatives.

Purifine PLC
Increase yield of degummed and refined seed oils
Developed specifically for processing high
phosphorus oils
Oil treated with PLC can be easily refined to exceed
standard quality specifications
Reduces oil losses and waste streams
Implementation costs are quickly paid back through
yield gains
Process is compatible with most current refining
operations

How does Purifine PLC

degumming works
How does Purifine PLC work?

oil soluble

water soluble

emulsifier

Purifine PLC converts phospholipids to oil

Purifine PLC
Separates the oil component
from the water soluble
component (diacylglycerol)

Phospholipids in oil reduce

the separation efficiency
of the oil and water (gum)
phases. Phospholipids
comprise an oil-soluble
backbone, and a watersoluble head group.

The addition of water to crude

vegetable oil results in the
hydration of the phospholipids.
The molecules move to the oilwater interface, holding the two
phases together as an emulsion
or gum.

Purifine PLC

Dramatically reduces the

volume and emulsifying
properties of gum

Allows cleaner separation of

oil and water phases

Significantly reduces oil lost

in the gum fraction
Following a centrifuge process
to separate the gum fraction
from the oil, neutral oil
(triacylglycerol) is entrained with
the intact phospholipids. The
entrained neutral oil represents
a yield loss during degumming.

Further increases oil yield by

capture of 1,2-diacylglycerol
in the oil phase

key

phospholipid

triacylglycerol (TAG)

diacylglycerol (DAG)

phosphate ester

Modified water degumming

with Purifine PLC
c
c

key

crude oil

purifine PLC

water

steam

Optimized process performance means maximum yield. To optimize

the Purifine PLC degumming process, the enzyme is given the right
environment and equipment to do its job. Performance can be monitored
at control points installed at critical stages in the process.

gums
c

pump

control point

The key considerations are

Oil temperature
Enzyme and water dose matched to oil flow rate
Creation of a fine emulsion with a high shear mixer
Sufficient residence time for the enzyme to complete its work in breaking
down the emulsion

Measuring process efficiency

Visual inspection of heavy phase confirms PLC reaction is occurring,
through a low volume of fluid and elastic gums
Residual phosphorus in oil confirms process efficiency
Mass flow and moisture content can be used to calculate yield
improvements
Fatty matter balance using inventories can consolidate average plant
performance

Purifine PLC maximizes

yield gain
Water degumming losses increase with increase in P in oil
Degumming oil loss is proportional to the phospholipid content of the oil
Yield gain is proportional to P in crude oil
Increased operating margins

4
3.5

water
plc

yield losses (%)

3
2.5
2

oil

more oil

gum

less gum
*water

*water

1.5
1
0.5
0
200 400

600

p in crude oil (ppm)

800 1000

traditional
process:

improved
process:

no enzyme

Purifine PLC
enzyme

*excess water used for demonstration only

For further information, please visit www.dsm.com or contact:

DSM Food Specialties
P.O. Box 1
2600 MA Delft
The Netherlands
Tel: +31 (0) 15 279 3474
Email: info.food@dsm.com

For DSM, quality is a way of life. This is the core of Quality for Life.
Quality for Life is the mark of quality, reliability and traceability. It means that DSM customers
are getting the best nutrition & health ingredients, knowing the source on which they depend.
Quality for Life means sustainability. It symbolizes our commitment to our environment, consumers,
our business partners, our people and the regulatory framework that governs our operations.
With the Quality for Life seal, we guarantee peace of mind for you and for your customers.

Although diligent care has been used to ensure that the information provided herein is accurate, nothing contained herein can be construed to imply any
representation or warranty for which we assume legal responsibility, including without limitation any warranties as to the accuracy, currency or completeness of
this information or of non-infringement of third party intellectual property rights. The content of this document is subject to change without further notice. Please
contact us for the latest version of this document or for further information. Since the users product formulations, specific use applications and conditions of use
are beyond our control, we make no warranty or representation regarding the results which may be obtained by the user. It shall be the responsibility of the user to
determine the suitability of our products for the users specific purposes and the legal status for the users intended use of our products.
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The applicability of any other terms and conditions is explicitly rejected and superseded by our General Terms and Conditions of Sale. The current version of our
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