RESEARCH PAPER
The
effect
of
foliar
application
of
microelements
on
Abstract
In order to investigate on the effect of foliar application of microelements on phenological and physiological
characteristics of Mung bean under drought stress, factorial experiment based on completely randomized design
with three replications was conducted in the Agriculture Faculty greenhouse of Islamic Azad University of
Varamin-Pishva branch in2013-2014. The first factor was drought stress at four levels include a control (without
stress), applying of drought stress at flowering stage, pod and dough stages and the second factor was foliar
application of microelements at three levels include a control (spraying of distilled water), 3 and 6 pm. The
results showed that the drought stress decreases plant height, leaf area index, thousand grain weight, chlorophyll
a, chlorophyll b and total chlorophyll and increases grain protein percentage and pro line accumulation. The
effect of drought stress at flowering stage was higher than other stages. Also, the foliar application of
microelements was increased the plant height, leaf area index, thousand grain weight, chlorophyll a, chlorophyll b
and total chlorophyll, grain protein percentage and pro line accumulation in with and without stress conditions.
Therefore, the most effective concentration of microelements was 6 pm.
* Corresponding
molybdenum,
planting
was
microelements
boron
of
also
seeds
reported
manganese
were
obtained
that
increases
and
the
the
chlorine
from
foliar
application
yield
and
of
yield
plant
height
decreases
under
drought
stress
with
three
replications
in
Agriculture
Faculty
three
(foliar
concentrations
in
clued
control
respectively.
For planting of Mung bean in green house, 36 plastic
pots with 30 cm height and25 cm diameter were
Statistical analysis
levels of 0.05.
Results
Plant height
stress
and
foliar
application
of
Table 1. Physical and chemical characteristics of the used soilin the pots for Mung bean. Cultivation.
Tissue
pH
Silt-Loam 7
EC
OC
Clay
Silt
Sand N
(ds/m) (%)
(%)
(%)
(%)
0.35
20
20
60
3.3
0.5
6.4
155
0.83
Zn
Se
0.58
12.2
the
control
by
pm
foliar
application
of
and
foliar
application
of
The
drought
stress
Table 2. The variance analysis of microelements on phenological and physiological characteristics of Mung bean
plant under drought stress.
S.O.V
df
Plant height
seed weight
Seed protein
Chlorophyll a
Chlorophyll b
Total
Proline
chlorophyll
Application (a)
13.66**
3.08**
69.75**
7.83**
0.02**
0.18**
0.35**
427.86**
12.72**
0.14**
54.91**
15.92**
0.008**
0.05**
0.1**
5.78**
a*b
1.2ns
0.041*
5.13*
0.94*
0.001**
0.005*
0.01**
0.48*
Error
24
2.47
0.015
2.08
0.35
0.0003
0.001
0.003
0.17
5.23
4.41
12.89
3.33
2.41
2.43
2.2
4.07
CV%
Fig.
4.
The
effect
of
foliar
application
of
2.
The
effect
of
foliar
application
of
Fig.
Fig.
3.
The
effect
of
foliar
application
of
5.
The
effect
of
foliar
application
of
concentration
is
increases
in
response
to
environmental stress.
Discussion
During the water stress, photosynthesis is limited by
stomata closure. Studies showed that water deficiency
enzyme
activity
transmission
and
lipid
ultimately
reported
The
soil.
by
capacity
bilayermembrane
Costa
Therefore,
the
that
and
Morel
plant
has
(1994).
microelements
Fig.
8.
The
effect
of
foliar
application
of
6.
The
effect
of
foliar
application
of
the
photo-assimilates
production
Fig.
7.
The
effect
of
foliar
application
of
Fig.
9.
The
effect
of
foliar
application
of
showed
that
the
drought
stressed
creases
transgenic
Arabidopsis
caused
changes
in
the
128, 1255-1263.
Drought stress increases the oxygen free radicals in
the chloroplast and causes to destruction of the
active
chlorophyll
separating of
oxygen
destruction
species.
Also,
was performed
the
with
of
(Benedetti
chlorophyll
durability
chlorophylls
and
preservation
in
drought
enzymes
Arruda,
and
stress
genes
2002).
are
Leaf
photosynthesis
are
physiological
using
and
of
nutrient
containing
with
iron
570.
PS,
Dinesk
K,
Badole
WP,
48, 541-543.
Bahrani
AH,
Abad
HHS,
Savestani
ZT,
Azerbaijan.
M.Sc.
Theses,
Tarbiat
Modares
Proceeding of
6th
University, Iran. 28 p
Australian
Journal
Agriculture
J.
2003.
seed
Efficient
fertilizer
use
125-139.
431.
Umar S. 2006. Alleviating adverse effects of water
stress on yield of sorghum, mustard and groundnut
by potassium application. Pakistan Journal of Botany
38, 1373-1380.
2002.
RFLP-facilitated
investigation
of
the
Physiology.
(Nilaparvatalugens).
Second Edition,
Theoretical
and
Applied